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All Journal Jurnal Sain Veteriner Jurnal Veteriner Media Kedokteran Hewan Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Jurnal Kedokteran Hewan
Bambang Sektiari Lukiswanto
Departemen Klinik Veteriner Fakultas Kedokteran Hewan Universitas Airlangga, Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Encoding Gene Identification in Staphylococcus aureus enterotoxin B from Nasal Mucosa of the Dog Arifah, Sitti; Effendi, Mustofa Helmi; Sektiari, Bambang
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 4 No. 1, JANUARY 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v4i1.9241

Abstract

This research aimed to know the enterotoxin B in Staphylococcus aureus isolated from the nasal mucosa of the dog. Six of fifteen samples have characteristics like cocci shaped, Gram-positive, mannitol fermented, produce catalase, coagulase and acetyl methyl carbinol. Then to identify enterotoxin B in 6 isolates of Staphylococcus aureus using PCR techniques. Result of the identification of gene that are 50% positive samples had enterotoxin B. Further research is needed on high sensitivity antibiotic for treatment of the caused by Staphylococcus aureus infections, as well as the need cautious to the spread of enterotoxin B in Staphylococcus aureus. 
Ekspresi IL – 1β pada Proses Kesembuhan Fraktur Os Femur Tikus Putih (Rattus norvegicus) Ovariektomi yang Diterapi dengan Ekstrak Cikal Tulang (Cissus quadrangularis) Ira Sari Yudaniayanti; Bambang Sektiari Lukiswanto; M. Zainal Arifin
Jurnal Sain Veteriner Vol 35, No 1 (2017): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2018.61 KB) | DOI: 10.22146/jsv.29296

Abstract

This study aims to determine the potential of the Cissus quadrangularis extract to decreased the expression of IL-1 β so that it can to inhibit bone resorption and osteoporosis fracture healing is not delayed union. Forty rats adapted for 1 week, and then divided into four groups with 10 replications. P0 (sham-operated + Osteotomy + CMC Na); P1 (ovariectomy + Osteotomy + CMC Na); P2 (ovariectomy + Osteotomy + Raloxifene 5.4 mg /kg); P3 (ovariectomy + Osteotomy + CQ 750 mg / kg). Osteoporosis induced is made by bilateral ovariectomy in rats, then to make sure that osteoporosis is already happened, 8 weeks post-ovariectomy performed radiology examinations on the femur, then the osteotomy action. IL1-β expression observations performed in at the 2nd week and 6th week after osteotomy through the immunohistochemistry examination. The results of immunohistochemistry examination in the 2nd week showed an increased expression of IL1-β in all groups of rat that did ovariectomy (P1, P2 and P3) were significantly different (p <0.05) with a rat group that didn’t ovariectomy, as well P2 dan P3 were significantly different with P1. The Results of immunohistochemistry examination in the 6th week showed the samepattern with the 2nd week and there is increased IL1-β expression in all groups of rat compared with the results of the examination in the 2nd week. Based on these results it can be concluded that Cissus quadrangularis extract andRaloxifen can decreased the expression of IL-1 β, and the results that don’t significantly different, so that these both materials can to inhibit bone resorption and osteoporosis fracture healing don’t get delayed union.
Pengaruh Ekstrak Buah Delima Terstandar 40% Ellagic Acid terhadap Profil Darah Tikus Putih Yang Mengalami Nefrotoksisitas akibat Induksi Gentamisin Bambang Sektiari Lukiswanto; Wiwik Misaco Yuniarti
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7994.355 KB) | DOI: 10.22146/jsv.34697

Abstract

The purpose of this study was to determine the effect of pomegranate extract of 40% ellagic acid on blood profile of albino rats that had nephrotoxicity after included with Gentamicin. a total of 32 white rats were divided into four treatment groups, each consisting of eight rats. the four treatment groups were healthy rats (P0), Gentamicin induced rats (P1), Gentamicin induced rats and ellagic acid treatment (P2) and Gentamicin induced rats and standardized Pomegranate extract therapy 40% ellagic acid treatment (P3). After seven days of treatment, blood was taken for blood profile tests. The results showed that in the Gentamicin-induced group (P0), a decrease in blood profile allegedly caused by Gentamicin that could cause damage and impaired renal tubular function. Givibg ellagic acid and pomegranate extracts of 40% ellagic acid proved to improve the blood profile of Gentamicin induced rats. This is due to inhibition of renal damage and improved renal function due to antioxidant activity possessed by ellagic acid and pomegranate extract of standardized 40% ellagic acid.
Aktivitas Antifibrotik Ekstrak Buah Delima Terstandar 40% Ellagic Acid pada Tikus Putih (Rattus norvegicus) sebagai Hewan Model (ANTIFIBROTIC ACTIVITY OF POMEGRANATE FRUIT EXTRACT STANDARDIZED 40% ELLAGIC ACID ON RATS (RATTUS NORVEGICUS) AS ANIMAL MODEL) Wiwik Misaco Yuniarti; Bambang Sektiari Lukiswanto
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.605 KB)

Abstract

The number of patients with chronic liver disease (fibrosis or cirrhosis of the liver) is increasing fromtime to time. However, until now there is no therapy that really effective to overcome that disease. Therapyfor liver fibrosis typically use substance that have antioxidant activity, anti-inflammatory or anti fibrotic.Various efforts always done to find alternative therapies for liver fibrosis. One of them is the use ofpotential plants that suspected having such activity. Various parts of pomegranate have been shown tohave various activities that beneficial for health. This study was conducted to determine the effect ofpomegranate fruit extract standardized 40% ellagic acid on the improvement degree of liver fibrosis causedby cholestasis by measuring of serum alkaline phosphatase levels (ALP) and gamma-glutamyl transferase(GGT), as a specific indicator of liver damage becaused of cholestasis. The research was conducted by using32 male rats, wistar strain, 2.5 month old, weighing between 150-200 grams. Animal models of liver fibrosis obtained by using BDL technique. Subjects were divided into a control group (P0 = without BDLand giving of pomegranate extract) and treatment groups (P1 = BDL with administration of CMC, P2 =BDL with ellagic acid 90% and P3 = BDL with pomegranate fruit extract standardized 40% ellagic acid).CMC, extract (150 mg / kg BW / PO) and ellagic acid (60 mg / kg BW / PO) administered for 21 consecutivedays in the same volume. At the end of 21 days periods, biochemical evaluation was performed to measureserum levels of GGT and ALP. The result indicated that administration of pomegranate fruit extract ( P3)significantly reduced GGT ( 10.5±9.2 mg/dl) and ALP level ( 509.0±4.2 mg/dl) close to normal level of GGTand ALP ( P0, GGT : 2.8 ± 1.4; ALP : 449.0±62.3) (p<0.05). The level of GGT and ALP in P3 group were lowercompared to the group ellagic acid (P2, GGT=48.5±4.8 and ALP = 691.0± 29.7) and group which only begiven CMC (P1, GGT 191.0±35.4 and ALP 890 ± 5.7) ( p<0.05). Extract of pomegranate fruit thatstandardized with 40% ellagic acid is potential as a antifibrotic agent.
HYSTOPATHOLOGIC CHANGES ON AORTA OF CIRRHOSIS MALE RATS (RATTUS NORVEGICUS) INDUCTED BY ESCHERICIA COLI O55 : B5 Tony Hartono; Wiwik Misaco Yuniarti; Bambang Sektiari Lukiswanto
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (811.098 KB)

Abstract

The background of this study is to visualize histopathological changes on aorta of cirrhosis rats (Rattus norvegicus) induced by endotoxin E. coli O55 : B5 This study was a laboratory experimental using complete randomized design with five treatments and five repetitions. Twenty five male Wistar rats were used as experimental model of cirrhosis by bile duct ligation (BDL) technique. Three weeks after BDL, all cirrhosis experimental models were induced with a single intra venous injection of Eschericia coli endotoxin (3mg/kg b.b in 1 ml sterile saline), except those of five control rats that induced with sterile saline at the same volume only. Aortas of control rats group were excised at 6 hours after induction with sterile saline, whereas the other four groups were done at 6, 12, 18 and 24 hours after induction with endotoxin. The quantity of endothelial cell, discontinuity increment and the thickness of internal elastic lamina layer were observed to know histopathological changes on aorta. Histopathological changes were observed using a light mycroscope, dyscontinuity and thickness of internal elastic lamina were measured by reticular micrometer. The quantity of endothelial cell on control and observation interval of 6 and 12 hours as significant difference (P<0.05), which are bigger than that of 18 and 24 hours. Rats in the control group have the biggest quantity comparing to the other treatments. Discontinuity and thickness of internal elastic lamina layer had significant difference (P<0.05) on control, observation on 6 and 12 hours compared to observation on 18 and 24 hours after being induced with endotoxine. The highest discontinuity and the thinnest elastic lamina internal were obtained within observation on 24 hours. VCAM-1 expression on control group differ from observation on 6 and 12hours but all of them have significant difference to observation on 18 and 24 hours (P<0,05). The decrease of endothelial cell number is caused by endothelial cell contact with endotoxin. Longer contact interval can make more severe injury and dysfunction. When the aorta had loss of its endothelial cell, internal elastic lamina will exposed with endotoxin directly. In the vessel, endotoxin cause nitric oxide (NO) release, that have antiproliferative characteristic for neointimal formation. This condition presumed to be the cause of the dyscontinuity increment and thickness decrement of internal elastic lamina of cirrhosis rats. VCAM-1 expression was influenced by quantity and condition of endothelial cell. The higher VCAM-1 expression in control group higher than the other group because the quntity and morphofunction of endothelial cell was better than that of 6, 12, 18 and 24 hours.
Encoding Gene Identification in Staphylococcus aureus enterotoxin B from Nasal Mucosa of the Dog Sitti Arifah; Mustofa Helmi Effendi; Bambang Sektiari
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 4 No. 1, JANUARY 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v4i1.9241

Abstract

This research aimed to know the enterotoxin B in Staphylococcus aureus isolated from the nasal mucosa of the dog. Six of fifteen samples have characteristics like cocci shaped, Gram-positive, mannitol fermented, produce catalase, coagulase and acetyl methyl carbinol. Then to identify enterotoxin B in 6 isolates of Staphylococcus aureus using PCR techniques. Result of the identification of gene that are 50% positive samples had enterotoxin B. Further research is needed on high sensitivity antibiotic for treatment of the caused by Staphylococcus aureus infections, as well as the need cautious to the spread of enterotoxin B in Staphylococcus aureus. 
Identification of Worm In Digestive Tract of Consumed Cattle Egret (Bubulcus ibis) In Ujung Pangkah, Gresik Nisa’ Rachmaningtyas Putri; E Djoko Poetranto; Nunuk Dyah Retno Lastuti; Setiawan Koesdarto; Bambang Sektiari Lukiswanto; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.189 KB) | DOI: 10.20473/jops.v2i1.16382

Abstract

Ujung Pangkah, Gresik was became the most habitat of fowls such as Cattle egret (Bubulcus ibis). The aim of this research were to find out the type of worm that infected digestive tract of Cattle egret (Bubulcus ibis) in Ujung Pangkah, Gresik. The examination of worm in digestive tract was done by dissection and fecal examination. The identification based on morphological of the eggs and worms. The samples of digestive tract organ collected from restaurant that preserve Cattle egret as a main menu. Whereas the fecal sample collected by swab from the intestine. Cattle egret (Bubulcus ibis) has positive infection by worm parasite if there were worm or egg inside the digestive tract. Fecal samples were examined by native, sedimentation, coconut-sugar floating method and EPG counting with Lucient Brumpt. And the worm morphology from dissection of digestive tract would be identify with source book, journal, and discussed with supervisor. The result showed that 20 of digestive tract dissection and 20 fecal samples, there were three types of worm, Contracaecum sp., Cosmocephalus sp., Capillaria sp. and egg of Contracaecum sp. with 200 EPG
The Potency of Anting Anting (Acalypha indica L.) Leaf Extract as An Acaricide on Boophilus microplus in Larvae and Adult Stages In Vitro Fitri Nurdiana; Rahmi Sugihartuti; Bambang Sektiari Lukiswanto
Journal of Parasite Science (JoPS) Vol. 5 No. 1 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (483.982 KB) | DOI: 10.20473/jops.v5i1.29955

Abstract

The purpose of this study was to determine the potency of anting-anting leaf extract (Acalypha indica L.) as an acaricide against larval and adult Boophilus microplus in vitro by observing the mortality rate of B. microplus. The present study design was a completely randomized design. This study used five treatments, including: Tween 80 1% and aquadest (K-), Neguvon (K+), anting-anting leaf extract with a concentration of 3.125% (P1), 6.25% (P2), and 12.5% (P3 ). Each treatment used 5 repetitions and each repetition used 6 B. microplus larval stages and 5 B. microplus adults. Observations were made for 5 hours for the larval stage of B. microplus and 24 hours for the adult stage of B. microplus. Boophilus microplus is declared dead if there is no movement at all when touched with a needle. The data obtained were analyzed using factorial ANOVA and continued with Duncan's multiple distance test. The results showed that the 12.5% anting anting leaf extract treatment showed no significant difference with the treatment using Neguvon. (p>0.05) in larval stage B. microplus, but in adult B. microplus showed a significant difference with Neguvon (p<0.05). The results of the statistical test can be concluded that the extract of anting-anting leaf (A. indica L.) has the potential as an acaricide of B. microplus larval stage in vitro. The higher the concentration of anting anting leaf extract, the higher the acaricide activity.
PENGUKURAN NILAI KONDISI TERNAK (NKT), CONCEPTION RATE (CR), SERVICE PER CONCEPTION (S/C) PADA SAPI CROSSBREED LIMOUSIN DI KECAMATAN BALONGPANGGANG KABUPATEN GRESIK Fajar Septian H; Imam Mustofa; Bambang Sektiari L.
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (468.54 KB) | DOI: 10.20473/ovz.v8i1.2019.76-81

Abstract

This research was conducted to find out the Body Condition Score (BCS, in nine scale) of 30 Limousine crossbred cows in Balongpanggang, Gresik regency at the time of  Artificial Insemination,  21 and 60 days later. Questionnaires obtained to identify the knowledge of farmers and inseminators in estrous detection. Conception Rate (CR) and Service per Conception (S/C) counted based on pregnancy diagnosis through rectal palpation 60 days post insemination. The result showed that the average of BCS in pregnant cows at 60 days post insemination was highest compared to the others. Conception rate and service per conception of the cows were 60% and 1.63 respectively, meanwhile the knowledge of estrous detection of farmers was lower compared to those of inseminator. It could be concluded that implementation of AI technique in Limousine crossbred cows at Balongpanggang, Gresik regency was good enough, but the knowledge of farmers about estrous detection needs to be improved.
IDENTIFIKASI PROTEIN EPIDERMAL GROWTH FACTOR (EGF) 46 kDa HASIL MATURASI OOSIT SAPI SECARA IN VITRO Widjiati w; Anike Rachmawati; Sri Mumpuni; Bambang Sektiari
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.059 KB) | DOI: 10.21157/j.ked.hewan.v6i1.734

Abstract

Tujuan dari penelitian ini adalah mengidentifikasi suatu protein dengan berat molekul 46 kDa yang diduga sebagai epidermal growth factor (EGF) yang diisolasi dari oosit sapi yang telah dimaturasi secara in vitro dengan metode elektroforesis. Ovarium sapi yang berasal dari rumah potong hewan, diaspirasi pada folikel dengan diameter permukaan ≤5 mm menggunakan spuit dan jarum. Oosit dimaturasi dalam tissue culture medium (TCM) 199 selama 22 jam pada suhu 38,5° C di dalam inkubator CO2. Preparasi protein dengan berat molekul 46 kDa menggunakan sodium dodecyl sulphate polyacrilamide gel elektroforesis (SDS PAGE). Berdasarkan perhitungan jumlah regresi dari protein marker, didapatkan 12 fraksi protein yaitu BM 172,7; 153,09; 118,24; 102,43; 89,27; 59,75; 46,41; 43,82; 40,2; 36,06; 23,45; dan 18,42 kDa. Protein dengan berat molekul 46,41 kDa yang tampak pada pita protein dapat diidentifikasi sebagai protein yang diduga EGF yang berperan dalam proses maturasi oosit.
Co-Authors A'la, Rofiqul Al-Arif, Moh. Anam Anike Rachmawati Arifah, Sitti Aswin, Ahmad Aulia Rakhman, Muhammad Andika Bagaskara, Ryan Boedi Setiawan BUDI UTOMO Diyantoro Diyantoro, Diyantoro E Djoko Poetranto Eduardus Bimo Aksono Herupradoto Fajar Septian H Fedik Abdul Rantam Fitri Nurdiana Helen Susilowati, Helen Hisyam, Mirza Atikah Madarina Imam Mustofa Ira Sari Yudaniayanti Ira Sari Yudaniayanti Ira Sari Yudaniyanti Kadek Rachmawati Kuncorojakti, Suryo Kusnoto Kusnoto LINA SUSANTI Lucia Tri Suwanti, Lucia Tri M. Zainal Arifin Ma'ruf, Anwar Mufasirin Mustofa Helmi Effendi Niken Hastuti, Apriliwiyani Nisa’ Rachmaningtyas Putri Nove Hidajati Nufus, Faizah Zakiyyatun Nunuk Dyah Retno Lastuti Nusdianto Triakoso Poetranto, Emmanuel Djoko Rahardjo, Adi Prijo Rahmahani, Jola Rahmi Sugihartuti Setiawan Koesdarto Sitti Arifah Soeharsono Sri Mumpuni Sudjono, Budi Santoso Suryaningtyas, Ignasia Friska Amelia Theresia, Sis Wisnuarti Tony Hartono Widiyatno, Thomas Valentinus Widjiati w Wijaya, Andi Yasmin Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti