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All Journal Jurnal Sain Veteriner Media Kedokteran Hewan Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Jurnal Kedokteran Hewan

Bambang Sektiari Lukiswanto

Departemen Klinik Veteriner Fakultas Kedokteran Hewan Universitas Airlangga, Surabaya

Author-ID : 2896040

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Encoding Gene Identification in Staphylococcus aureus enterotoxin B from Nasal Mucosa of the Dog Arifah, Sitti; Effendi, Mustofa Helmi; Sektiari, Bambang
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 4 No. 1, JANUARY 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v4i1.9241

This research aimed to know the enterotoxin B in Staphylococcus aureus isolated from the nasal mucosa of the dog. Six of fifteen samples have characteristics like cocci shaped, Gram-positive, mannitol fermented, produce catalase, coagulase and acetyl methyl carbinol. Then to identify enterotoxin B in 6 isolates of Staphylococcus aureus using PCR techniques. Result of the identification of gene that are 50% positive samples had enterotoxin B. Further research is needed on high sensitivity antibiotic for treatment of the caused by Staphylococcus aureus infections, as well as the need cautious to the spread of enterotoxin B in Staphylococcus aureus.Â

Ekspresi IL – 1β pada Proses Kesembuhan Fraktur Os Femur Tikus Putih (Rattus norvegicus) Ovariektomi yang Diterapi dengan Ekstrak Cikal Tulang (Cissus quadrangularis) Ira Sari Yudaniayanti; Bambang Sektiari Lukiswanto; M. Zainal Arifin
Jurnal Sain Veteriner Vol 35, No 1 (2017): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study aims to determine the potential of the Cissus quadrangularis extract to decreased the expression of IL-1 β so that it can to inhibit bone resorption and osteoporosis fracture healing is not delayed union. Forty rats adapted for 1 week, and then divided into four groups with 10 replications. P0 (sham-operated + Osteotomy + CMC Na); P1 (ovariectomy + Osteotomy + CMC Na); P2 (ovariectomy + Osteotomy + Raloxifene 5.4 mg /kg); P3 (ovariectomy + Osteotomy + CQ 750 mg / kg). Osteoporosis induced is made by bilateral ovariectomy in rats, then to make sure that osteoporosis is already happened, 8 weeks post-ovariectomy performed radiology examinations on the femur, then the osteotomy action. IL1-β expression observations performed in at the 2nd week and 6th week after osteotomy through the immunohistochemistry examination. The results of immunohistochemistry examination in the 2nd week showed an increased expression of IL1-β in all groups of rat that did ovariectomy (P1, P2 and P3) were significantly different (p <0.05) with a rat group that didn’t ovariectomy, as well P2 dan P3 were significantly different with P1. The Results of immunohistochemistry examination in the 6th week showed the samepattern with the 2nd week and there is increased IL1-β expression in all groups of rat compared with the results of the examination in the 2nd week. Based on these results it can be concluded that Cissus quadrangularis extract andRaloxifen can decreased the expression of IL-1 β, and the results that don’t significantly different, so that these both materials can to inhibit bone resorption and osteoporosis fracture healing don’t get delayed union.

Pengaruh Ekstrak Buah Delima Terstandar 40% Ellagic Acid terhadap Profil Darah Tikus Putih Yang Mengalami Nefrotoksisitas akibat Induksi Gentamisin Bambang Sektiari Lukiswanto; Wiwik Misaco Yuniarti
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The purpose of this study was to determine the effect of pomegranate extract of 40% ellagic acid on blood profile of albino rats that had nephrotoxicity after included with Gentamicin. a total of 32 white rats were divided into four treatment groups, each consisting of eight rats. the four treatment groups were healthy rats (P0), Gentamicin induced rats (P1), Gentamicin induced rats and ellagic acid treatment (P2) and Gentamicin induced rats and standardized Pomegranate extract therapy 40% ellagic acid treatment (P3). After seven days of treatment, blood was taken for blood profile tests. The results showed that in the Gentamicin-induced group (P0), a decrease in blood profile allegedly caused by Gentamicin that could cause damage and impaired renal tubular function. Givibg ellagic acid and pomegranate extracts of 40% ellagic acid proved to improve the blood profile of Gentamicin induced rats. This is due to inhibition of renal damage and improved renal function due to antioxidant activity possessed by ellagic acid and pomegranate extract of standardized 40% ellagic acid.

Encoding Gene Identification in Staphylococcus aureus enterotoxin B from Nasal Mucosa of the Dog Sitti Arifah; Mustofa Helmi Effendi; Bambang Sektiari
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 4 No. 1, JANUARY 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v4i1.9241

Identification of Worm In Digestive Tract of Consumed Cattle Egret (Bubulcus ibis) In Ujung Pangkah, Gresik Nisa’ Rachmaningtyas Putri; E Djoko Poetranto; Nunuk Dyah Retno Lastuti; Setiawan Koesdarto; Bambang Sektiari Lukiswanto; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Ujung Pangkah, Gresik was became the most habitat of fowls such as Cattle egret (Bubulcus ibis). The aim of this research were to find out the type of worm that infected digestive tract of Cattle egret (Bubulcus ibis) in Ujung Pangkah, Gresik. The examination of worm in digestive tract was done by dissection and fecal examination. The identification based on morphological of the eggs and worms. The samples of digestive tract organ collected from restaurant that preserve Cattle egret as a main menu. Whereas the fecal sample collected by swab from the intestine. Cattle egret (Bubulcus ibis) has positive infection by worm parasite if there were worm or egg inside the digestive tract. Fecal samples were examined by native, sedimentation, coconut-sugar floating method and EPG counting with Lucient Brumpt. And the worm morphology from dissection of digestive tract would be identify with source book, journal, and discussed with supervisor. The result showed that 20 of digestive tract dissection and 20 fecal samples, there were three types of worm, Contracaecum sp., Cosmocephalus sp., Capillaria sp. and egg of Contracaecum sp. with 200 EPG

The Potency of Anting Anting (Acalypha indica L.) Leaf Extract as An Acaricide on Boophilus microplus in Larvae and Adult Stages In Vitro Fitri Nurdiana; Rahmi Sugihartuti; Bambang Sektiari Lukiswanto
Journal of Parasite Science (JoPS) Vol. 5 No. 1 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study was to determine the potency of anting-anting leaf extract (Acalypha indica L.) as an acaricide against larval and adult Boophilus microplus in vitro by observing the mortality rate of B. microplus. The present study design was a completely randomized design. This study used five treatments, including: Tween 80 1% and aquadest (K-), Neguvon (K+), anting-anting leaf extract with a concentration of 3.125% (P1), 6.25% (P2), and 12.5% (P3 ). Each treatment used 5 repetitions and each repetition used 6 B. microplus larval stages and 5 B. microplus adults. Observations were made for 5 hours for the larval stage of B. microplus and 24 hours for the adult stage of B. microplus. Boophilus microplus is declared dead if there is no movement at all when touched with a needle. The data obtained were analyzed using factorial ANOVA and continued with Duncan's multiple distance test. The results showed that the 12.5% anting anting leaf extract treatment showed no significant difference with the treatment using Neguvon. (p>0.05) in larval stage B. microplus, but in adult B. microplus showed a significant difference with Neguvon (p<0.05). The results of the statistical test can be concluded that the extract of anting-anting leaf (A. indica L.) has the potential as an acaricide of B. microplus larval stage in vitro. The higher the concentration of anting anting leaf extract, the higher the acaricide activity.

IDENTIFIKASI PROTEIN EPIDERMAL GROWTH FACTOR (EGF) 46 kDa HASIL MATURASI OOSIT SAPI SECARA IN VITRO Widjiati w; Anike Rachmawati; Sri Mumpuni; Bambang Sektiari
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): March
Publisher : Universitas Syiah Kuala

Tujuan dari penelitian ini adalah mengidentifikasi suatu protein dengan berat molekul 46 kDa yang diduga sebagai epidermal growth factor (EGF) yang diisolasi dari oosit sapi yang telah dimaturasi secara in vitro dengan metode elektroforesis. Ovarium sapi yang berasal dari rumah potong hewan, diaspirasi pada folikel dengan diameter permukaan ≤5 mm menggunakan spuit dan jarum. Oosit dimaturasi dalam tissue culture medium (TCM) 199 selama 22 jam pada suhu 38,5° C di dalam inkubator CO2. Preparasi protein dengan berat molekul 46 kDa menggunakan sodium dodecyl sulphate polyacrilamide gel elektroforesis (SDS PAGE). Berdasarkan perhitungan jumlah regresi dari protein marker, didapatkan 12 fraksi protein yaitu BM 172,7; 153,09; 118,24; 102,43; 89,27; 59,75; 46,41; 43,82; 40,2; 36,06; 23,45; dan 18,42 kDa. Protein dengan berat molekul 46,41 kDa yang tampak pada pita protein dapat diidentifikasi sebagai protein yang diduga EGF yang berperan dalam proses maturasi oosit.

The Efficacy of Madeira Vine Leaf Extract Combined with Turmeric Rhizome Extract Gel Topically on Second-Degree Burn Wound in Albino Rats Hisyam, Mirza Atikah Madarina; Yuniarti, Wiwik Misaco; Theresia, Sis Wisnuarti; Yudaniayanti, Ira Sari; Lukiswanto, Bambang Sektiari; Sudjarwo, Sri Agus; Widiyatno, Thomas Valentinus
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.91-98

Burns is now considered one of the most destructive forms of trauma affecting humans and animals. This study aimed to determine the effect of Madeira vine leaf extract combined with 2% Turmeric rhizome extract (MVLTR) gel administered topically on second-degree burn wound in albino rats based on the number of fibroblasts. A total of 25 male rats weighed 200–300 grams were divided into five groups i.e. (C-) was normal skin, (C+) was burn and treated using 1% silver sulfadiazine, (T1) was burn and treated using 1.25% MVLTR gel, (T2) was burn and treated using 2.5% MVLTR gel, and (T3) was burn and treated using 5% MVLTR gel. The burn wound was performed on the right gluteus of rats, and the therapy was applied twice a day topically, starting after the burn wounds were made. Skin samples were collected on day 15. The histopathological examination was performed by counting fibroblast cells and then analyzed using One-way ANOVA, continued with the Duncan test. Based on the results showed that the C- group was significantly different compared to C+, T1, T2, and T3 groups. C+ had a significant difference with the T1 and T3 groups, meanwhile not significantly different from the T2 group. T2 was not significantly different from the C+ and T3 groups. In conclusion, MVLTR gel at 5% concentration has the potential to be an effective treatment for second-degree burns, as indicated by the increased number of fibroblasts.

Detection of Spargana on Ptyas korros in Sidoarjo Bagaskara, Ryan; Lukiswanto, Bambang Sektiari; Rahardjo, Adi Prijo; Poetranto, Emmanuel Djoko; Koesdarto, Setiawan; Mufasirin, Mufasirin
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.122-125

Ptyas korros or Indo-China Rat Snake is one of the snakes that are commonly hunted in Indonesia. This study aimed to identify Spirometra sp. on P. korros in Sidoarjo. A total of 18 snakes were collected within 3 months. Identification of Spirometra sp. was done using a native method, Semichen Acetic Carmine, and HE staining. As a results, 8 (44.4%) of 18 samples showed positive infection with Spirometra sp. The results of this study indicate the presence of spargana in P. korros in Sidoarjo and no adult Spirometra sp. was found.

Strategic Infectious Diseases in Beef Cattle in Balongpanggang during 2023 Yuniarti, Wiwik Misaco; Lukiswanto, Bambang Sektiari; Setiawan, Boedi; Yudaniayanti, Ira Sari; Triakoso, Nusdianto; Hisyam, Mirza Atikah Madarina; Susanti, Lina; Niken Hastuti, Apriliwiyani; Sudjono, Budi Santoso
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.362-369

Strategic infectious animal diseases have a great impact on the national economy as they may cause great economic loss, public anxiety, and high mortality. In Indonesia, among the diseases considered as strategic infectious animal diseases is foot and mouth disease (FMD). This study reported the incidence of strategic infectious animal diseases in Balongpanggang district during 2023. Throughout 2023, FMD only accounted for 4% of the total diseases of the year and was the only strategic infectious animal disease reported that year. Meanwhile, bovine ephemeral fever (BEF) incidence reached 57% (876/1546) of the total diseases in beef cattle in 2023. In addition to BEF, official veterinary services reported a total case of 1546 throughout 2023 that comprises; diarrhea (99), limping (24), helminthiasis (91), ectoparasite (11), hematochezia (3), wound (3), mastitis (4), allergy (1), dystocia (28), vaginal prolapse (11), dermatitis (45), enteritis (27), tympani (11), scabiosis (122), arthritis (67), ophthalmic problem (11), endometritis (1), uterine retention (13), indigestion (2), pneumonia (21), hypoglycemia (5), abscess (21), ovariohysterectomy (1), orchitis (1), intoxication (1), and papilloma (1). In conclusion, the only strategic infectious animal disease reported in 2023 in Balongpanggang was FMD with the incidence as high as 3% as compared to the non-strategic infectious animal diseases, BEF, which reached 54% of the total cases during 2023.

Co-Authors A'la, Rofiqul Aksono HP., Eduardus Bimo Al-Arif, Moh. Anam Anike Rachmawati Anike Rachmawati Arifah, Sitti Aswin, Ahmad Aulia Rakhman, Muhammad Andika Bagaskara, Ryan Boedi Setiawan BUDI UTOMO Diyantoro Diyantoro, Diyantoro E Djoko Poetranto Fedik Abdul Rantam Fitri Nurdiana Helen Susilowati, Helen Hisyam, Mirza Atikah Madarina IMAM MUSTOFA Ira Sari Yudaniayanti Ira Sari Yudaniyanti Kadek Rachmawati Kuncorojakti, Suryo Kusnoto Kusnoto LINA SUSANTI Lucia Tri Suwanti, Lucia Tri M. Zainal Arifin Ma'ruf, Anwar Mufasirin Mustofa Helmi Effendi Niken Hastuti, Apriliwiyani Nisa’ Rachmaningtyas Putri Nove Hidajati Nufus, Faizah Zakiyyatun Nunuk Dyah Retno Lastuti Nusdianto Triakoso Poetranto, Emmanuel Djoko Rahardjo, Adi Prijo Rahmahani, Jola Rahmi Sugihartuti Septian H, Fajar Setiawan Koesdarto Sitti Arifah Soeharsono Sri Mumpuni Sri Mumpuni Sudjono, Budi Santoso Suryaningtyas, Ignasia Friska Amelia Theresia, Sis Wisnuarti Tony Hartono Widiyatno, Thomas Valentinus Widjiati w Wijaya, Andi Yasmin Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti

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