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Uji Kinerja Konsorsium Bakteri (Bacillus coagulans, B. pumilus, B. subtilis, Nitrosomonas sp., B. licheniformis, Pseudomonas sp.) dalam Berbagai Bahan Pembawa (Alginat-Tepung Tapioka, Serbuk Talek-Gelatin, dan Tepung Beras-Susu Skim-Tepung Jagung-Dekstrosa) dan Waktu Kontak Terhadap Bioremediasi Perairan Asal Sungai Cimuka Diana Indah Permatasari; Ratu Safitri; Nia Rossiana; Bambang Priadie
BIOTIKA Jurnal Ilmiah Biologi Vol 12, No 2 (2014): BIOTIKA DESEMBER 2014
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v12i2.10077

Abstract

POTENSI BAKTERI PROBIOTIK ASAL BAYAM (Amaranthus tricolor L.) SEBAGAI ANTI Helicobacter pylori PENYEBAB GASTRITIS Rika Pura Berliyani; Sri Rejeki Rahayuningsih; Ratu Safitri
BIOTIKA Jurnal Ilmiah Biologi Vol 12, No 2 (2014): BIOTIKA DESEMBER 2014
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v12i2.10080

Abstract

Pengaruh Konsorsium Bakteri (Bacillus subtilis, B. pumilus, B. circulans, Nitrosomonas sp. dan Paenibacillus amyloliticus) dalam Berbagai Bahan Pembawa (alginat-susu skim, alginat-gelatin dan serbuk baglog-serbuk talek) Serta Waktu Kontak Terhadap Kemampuan Bioremediasi Perairan Asal Sungai Cimuka R. Ninda Naufal Utami; Ratu Safitri; Nia Rossiana; Bambang Priadie
BIOTIKA Jurnal Ilmiah Biologi Vol 12, No 1 (2014): BIOTIKA JUNI 2014
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v12i1.10072

Abstract

Suplementasi Probiotik dan Temulawak pada Ayam Pedaging terhadap Populasi Salmonella sp dan kolesterol darah (PROBIOTICS AND TEMULAWAK SUPPLEMENTATION ON BROILER CHICKENS AGAINST SALMONELLA SP POPULATION AND BLOOD CHOLESTEROL LEVEL) Kartiawati Alipin; Ratu Safitri; Ruhyat Kartasudjana
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (89.496 KB)

Abstract

Probiotics is a live microbial feed supplements that improve the microbial balance in the digestivetract of cattle host. Temulawak (Curcuma xanthorrhiza Roxb) is one of native Indonesian spices that areuseful for prevention and treatment of human disease but also can be used for broiler chickens supplement.Bioactive contents known acting as an anti-bacterial, anti-inflammatory, and anti-fungal. The study wasconducted to determine the effects of probiotic and temulawak supplementation on population of Salmonellasp and broiler chickens blood cholesterol. This study used 300 DOC final stock. Probiotic concentration 109cell/mL administered through drinking water every day while of temulawak extract dose 0.25 g/L 0.50 g/Lonce a week, for five weeks. The variables measured were Salmonella sp populations and blood cholesterollevels. The results showed that combination administered of probiotics with temulawak dose 0.50 g/Ldecreased of population of Salmonella amount to 87.04% and decreased of blood cholesterol amount21,48% at administered of probiotics with temulawak dose 0,25 g/L. The conclusions of this study showedthat the percentage decrease of Salmonella sp population and blood cholesterol levels in combinationprobiotic and temulawak supplementation so that broiler produced is safe for consumption.
Isolasi dan Karakterisasi Senyawa Kimia dari Kulit Batang Manggis (Garcinia mangostana Linn) Isolation and Characterization of Chemical Compound from Bark of Mangosteen (Garcinia mangostana Linn) Lia Destiarti; Ari Widiyantoro; Elvi Rusmiyanto; Maryati Maryati; Harlia Harlia; Ratu Safitri; Unang Supratman
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 7, No 2 (2009): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.098 KB) | DOI: 10.51850/jitkt.v7i2.231

Abstract

The purpose of this research is to isolate and characterize chemical compound from bark of mangosteen. Bark of mangosteen was macerated with methanol. The extract from that process was fractionated with n-hexane, methylen chloride, and ethyl acetate. Ethyl acetate fraction was separated and purified by vacuum column chromatography, gravitation column chromatography, and preparative thin layer chromatography. The relative pure compound was afforded from ethyl acetate fraction of mangosteen bark (8.5 mg) resulting from 1.5 kg of mangosteen bark. The yellow amorphous powder of compound melts at 114 ~ 116ºC (uncorrected). The purity of compounds was tested by 1 and 2 dimension thin layer chromatography which gave one spot on TLC plate. The ultraviolet-visible (in CH3OH solvent) spectrum showed absorption maximum at 318 nm (sinamoyl group/band I), 258 (shoulder), and 243 nm (benzoyl group/band II). Addition of NaOH caused batochromic shift of band I and band II predicted as two hydroxyl at C-4’ and C-7, respectively. The infrared spectrum displayed absorption bands of OH stretching at  3436 cm-1, C-H stretching at 2920 cm-1, C=O stretching at 1631 cm-1, C-O-C stretching at 1094 cm-1, and C-H aromatics bending at  970-800 cm-1. A molecular ion in the FABMS at m/z 271.36 [M+H] + was consistent for the molecular formula C15H10O5. The 1H NMR spectrum showed characteristic resonances of a flavone. Based on the result of phytochemical test and analysis of the spectrum, it is predicted that the compound belongs to flavone, a kind of flavonoids which has hydroxyl at C-5, C-7, and C-4.
Aplikasi Helicoverpa Armigera Nuclear Polyhedrosis Virus Subkultur (Hanpv1) pada Ectropis Burmitra Mia Miranti; Ratu Safitri; M Melanie; Nurullia Fitriani
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2016: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (477.241 KB)

Abstract

Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV) telah diproduksi pada larva Spodoptera litura. Virus hasil produksi tersebut adalah Helicoverpa armígera Nuclear Polyhedrosis Virus Subkultur atau HaNPV1. Penelitian dilakukan menggunakan metode deskriptif eksplorasi dengan menginfeksikan larva instar dua, tiga dan empat Ectropis burmitra (ulat jengkal daun teh) dengan sediaan HaNPV1 yang dioleskan pada pakan larva dengan konsentrasi virus yang digunakan sebesar 4 x 102, 4 x 104 dan 4 x 106 polihedra/ml. Hasil penelitian menunjukkan bahwa tingkat kematian larva instar dua, tiga dan empat Ectropis burmitra yangdiinfeksi virus dengan konsentrasi 4 x 102, 4 x 104 dan 4 x 106 polihedra/ml antara 80%-100%. Efektivitas infeksi HaNPV1 hingga 100% pada larva E. burmitra instar dua untuk semua konsentrasi infeksi. Akan tetapi semakin tua instar larva, tingkat kematian turun menjadi 80% pada larva instar tiga dan empat yang diinfeksi HaNPV1 dengan konsentrasi sebesar 4 x 102 polihedra/ml. Pada konsentrasi virus sebesar 4 x 106 polihedra/ml seluruh larva instar dua, tiga dan empat mencapai tingkat kematian 100%. Tingkat kematian yang tinggi terjadi pada larva ini karena E. burmitra masih satu ordo dengan H. armigera (sebagai inang utama HaNPV) dan S. litura yaitu Ordo Lepidoptera