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Peningkatan Populasi, Pertumbuhan dan Serapan Nitrogen Tanaman Kedelai dengan Pemberian Azotobacter Penghasil Eksopolisakarida Hindersah, Reginawanti; Rostini, Neni; Harsono, Arief; Nuryani, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.151 KB) | DOI: 10.24831/jai.v45i1.13801

Abstract

Nitrogen-fixing Azotobacter is widely used as biofertilizer in sustainable agriculture. The bacteria produce exopolysaccharide which might have a significant role in enhancing soybean nitrogen uptake and growth. The objective of this research was to obtain growth media of Exopolysaccharide–producing Azotobacter; and increase shoot and root growth as well as nitrogen uptake of soybean var. Anjasmoro at early vegetative phase following inoculation of Azotobacter chroococcum liquid. Research consist of two phase, 1) determination of organic-based media for A. chroococcum liquid inoculant production, and 2) pot experiment for application of liquid inoculant on soybean.  The first experiment was performed  in a series of  batch fermenter consisted of several organic media for 72 hours.  The second experiment was set in completely randomized design consisted of three density of liquid inoculant. The results verified that the best media which induced exopolysachharide production of A. chroococcum was 1% molase enriched with 0.1% NH4Cl. Liquid inoculant clearly enhanced population of Azotobacter in soybean rhizosphere, plant height, roots dry weight and N uptake of 21 day old soybean. This research implied that A. chroococcum might be used as biofertilizer at early growth of soybean. Keywords: Azotobacter chroococcum, biofertilizer, liquid inoculat
Peran Eksopolisakarida Azotobacter dan Bahan Organik untuk Meningkatkan Nodulasi dan Biomassa Kedelai pada Dua Ordo Tanah Hindersah, Reginawanti; Rostini, Neni; Kalay, Agustinus Marthin; Harsono, dan Arief
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 2 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.77 KB) | DOI: 10.24831/jai.v47i2.23328

Abstract

Exopolysaccharide (EPS) produced by nitrogen-fixing bacteria Azotobacter protect nitrogenase from oxygen. In legume,EPS plays a role in the immobilization of rhizobia to the roots. The objective of this experiment was to study the effect of EPSAzotobacter and organic matter on increasing number of nodules and biomass of soybeans grown in Inceptisols and Ultisols;as well as nitrogen-fixing bacteria population in soybean rhizosphere. The experiment was set up in a completely randomizedblock design with five replications to test combined treatments of two doses of crude EPS and organic matter. Nodule number,shoot dry weight and nitrogen uptake, as well as Azotobacter and Rhizobium population in soybean grown in Inceptisolsfollowing crude EPS and compost application, were higher than those grown in Ultisols. The application of EPS and compostIn Ultisols did not affect the number of nodule and other traits, but in Inceptisols, adding 6.25 g of compost and 20 mL of EPSto each plant increased the number of nodules and shoot weight at 42 days after planting. However, the highest N uptake wasdemonstrated by soybean received 10 mL and 20 mL EPS along with 12.5 g compost.