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KARAKTERISASI AWAL BAKTERIOSIN PRODUKSI BAKTERI ASAM LAKTAT YANG DIISOLASI DART PLIEK U = THE PRELIMINARY CHARACTERIZATION OF BAC 1ERIOCIN PRODUCED BY LACTIC ACID BACTERIA ISOLATED FROM PLIEK U Nurliana .; Suzanna Rabfiani; M. Hanafiah
Jurnal Sain Veteriner Vol 20, No 1 (2002): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5028.642 KB) | DOI: 10.22146/jsv.390

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Penelitian ini bertujuan untuk mengamati ciri-ciri senyawa aktif yang terkandung dalam supernatan bakteri asam laktat yang diisolasi dari pliek u akibat penambahan enzim proteolitik dan pemanasan. Pengujian ulang aktivitas hambatan dan uji sensitivitas supernatan isolat PBI (bakteri asam laktat) yang diduga mengandung bakteriosin menggunakan metode pour plate (overlay dan metoda sumur). Media untuk menumbuhkan isolat PBI digunakan MRS broth dimodifikasi dengan penambahan tween 80 dan yeast extract, dan juga menggunakan media agar MRS . Media untuk bakteri indikator (Listeria monocylogenes) menggunakan agar Bill 0.75 %. Pengaruh enzim pepsin dan tripsin kosentrasi 1 mg/ml setelah dicampur dengan supernatan isolat PBI (v/v) diuji terhadap sensitivitas bakteriosin. Uji sensitivitas terhadap pemanasan dilakukan pada suhu 60,80,100 dan 120°C selama 10 dan 12 menit, Data dianalisi secara deskriptif. Hasil penelitian menunjukkan bahwa supernatan isolat BAL PBI diduga mengeluarkan senyawa bioalctif (bakteriosin), karena mampu menghambat L. Monocytogenes dengan aktivitas hambatan sebesar 3 mm, dan juga sensitif terhadap pepsin dan tripsin, serta tidak sensitif pada pemanasan 60,80°C selama 10 dan 15 menit, tetapi sensitif pada pemanasan 100°C dan 120°C.
Pengaruh Infeksi Toxoplasma gondii Isolat Lokal terhadap Gambaran Darah pada MEncit (Mus musculus) Wisnu Nurcahyo; Dwi Priyo Widodo; M. Hanafiah
Jurnal Sain Veteriner Vol 22, No 1 (2004): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1694.608 KB) | DOI: 10.22146/jsv.469

Abstract

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VIABILITAS LARVA PADA NYAMUK Aedes aegypti, Aedes albopictus DAN Culex quinquefasciatus DENGAN BERBAGAI TINGKAT INFEKSI MIKROFILARIA (LARVA VIABILITY IN MOSQUITO Aedes aegypti, Aedes albopictus AND Culex quinquefasciatus WITH VARIOUS INFECTIONS OF MICROFILARIA) Auliya rahmi Ritonga; T. Fadrial Karmil; T. Zahrial Helmi; Winaruddin Winaruddin; M. Hanafiah; Razali Daud; M Daud AK
JURNAL ILMIAH MAHASISWA VETERINER Vol 5, No 1 (2020): NOVEMBER-JANUARI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v5i1.8578

Abstract

                Penelitian ini bertujuan untuk mengetahui viabilitas larva yang berperan aktif pada nyamuk Aedes aegypti, Aedes albopyctus dan Culex quinquefasciatus mulai dari larva satu hingga mencapai larva tiga atau larva infektif. Sampel yang digunakan dalam penelitian ini adalah anjing reservoir yang berjumlah 3 ekor dengan tingkat infeksi mikrofilaria 330 mf/ml darah, 1.430 mf/ml darah dan 10.395 mf/ml darah. Nyamuk yang diinfeksikan dengan berbagai tingkat infeksi mikrofilaremik tersebut diamati dan dilakukan pembedahan pada hari 1,3,6,9 dan 12. Hasil penelitian tersebut menunjukkan bahwa viabilitas larva pada nyamuk Ae. aegypti, Ae. albopyctus dan C. quinquefasciatus yang dilakukan penginfeksian dengan tingkat infeksi 330 mf/ml darah berkisar 80,08 %, 70,26 % dan 78,47 %; tingkat infeksi 1.430 mf/ml darah yaitu 65,72 %, 62,31 % dan 61,93 %; serta pada tingkat infeksi tinggi 10.395 mf/ml darah berkisar 53,92%, 55,79 % dan 54,27 %. Sehingga dapat disimpulkan bahwa ketiga jenis nyamuk tersebut berpotensi untuk menyebarkan penyakit dirofilariasis, hal ini sangat tergantung dari jumlah mikrofilaria yang terkandung dalam host defenitif. Semakin rendah tingkat infeksi maka semakin besar kemampuan nyamuk berpotensi menyebarkan penyakit.This study aims to determine the viability of the larvae that play an active role in Aedes aegypti mosquitoes, Aedes albopyctus and Culex quinquefasciatus from larvae one to reach the larvae of three or infective larvae. The samples used in this study were 3 reservoir dogs with microfilariae infection rate of 330 mf / ml of blood, 1,430 mf / ml of blood and 10,395 mf / ml of blood. Mosquitoes infected with various levels of microfilinemic infection were observed and performed surgery on days 1,3,6,9 and 12. The results showed that the viability of larvae in Ae mosquitoes. aegypti, Ae. albopyctus and C. quinquefasciatus infected with infection rate of 330 mf / ml of blood ranged 80.08%, 70.26% and 78.47%; infection rate 1.430 mf / ml of blood that is 65,72%, 62,31% and 61,93%; as well as at high infection rate 10,395 mf / ml of blood ranged 53.92%, 55.79% and 54.27%. So it can be concluded that the three types of mosquitoes have the potential to spread disease diropfilariasis, it is highly dependent of the number of microfilaria contained in the host defenitif. The lower the infection rate the greater the ability of mosquitoes to spread disease.
PENGARUH PAPARAN TIMBAL (Pb) TERHADAP LAJU PERTUMBUHAN IKAN NILA (Oreochromis nilloticus) Nur Inda Rahayu; Rosmaidar Rosmaidar; M. Hanafiah; T. Fadrial Karmil; T. Zahrial Helmi; Razali Daud
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 4 (2017): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.865 KB) | DOI: 10.21157/jim vet..v1i4.4757

Abstract

 Penelitian ini bertujuan mengetahui tingkat konsentrasi timbal   (Pb)  yang berpengaruh terhadap laju pertumbuhan ikan nila (Oreochromis nilloticus). Penelitian ini menggunakan ikan nila sebanyak 40 ekor dengan kriteria: sehat; bobot badan 15 – 18 gram; umur ± 2 bulan; jenis kelamin jantan. Penelitian ini menggunakan 4 kelompok perlakuan, P0 sebagai kontrol ikan hanya diberi pakan pelet, P1 diberikan paparan timbal  6,26 mg/L  dan pakan pelet, P2 diberikan paparan timbal 12,53 mg/L  dan pakan pelet dan P3 diberikan paparan timbal 25,06 mg/L  dan pakan pelet,  masing-masing perlakuan terdiri dari 10 ekor ikan nila. Perlakuan dilakukan selama 30 hari dan Pengukuran pertumbuhan ikan dilakukan setiap 10 hari sekali dengan cara menimbang bobot dan mengukur panjang tubuh setiap individu ikan. Hasil penelitian menunjukkan rata-rata panjang tubuh ikan nila P0 (9,45±1,06), P1 (8,89±0,90), P2 (8,86±0,87), dan P3 (8,66±0,85). Rata-rata berat ikan nila P0 (23,38±4,50), P1 (19,75±2,27), P2 (19,15±2,10), dan P3 (18,65±2,00). Laju pertumbuhan spesifik P0 (38,7%), P1 (8,3%), P2 (4,3%), dan P3 (3,3%). Laju pertumbuhan panjang harian individu ikan P0 (0,46 mm/d), P1 (0,27 mm/d), P2 (0,21 mm/d), dan P3 (0,19 mm/d). Kesimpulan dari penelitian bahwa konsentrasi timbal  dan lamanya paparan berpengaruh terhadap laju pertumbuhan ikan.  Konsentrasi timbal  yang sangat berpengaruh adalah 25,06 mg/L. ABSTRACTThe aims of this research was to find out to determine the level of Lead (Pb) concentration that affect the rate of growth tilapia (Oreochromis nilloticus). This study used 40 tilapia with criteria: healthy; body weight 15-18 gram; age ± 2 month; male sex. This study used 4 treatment groups, P0 as control, fish fed only pellets, P1 was given lead exposure 6,26 mg/L and pellet, P2 was given lead exposure 12,53 mg/L and pellet and P3 was given lead exposure 25,06 mg/L and pellet. Each treatment consisted of 10 tilapia fish. Treatment carried out for 30 days and measure every 10 days. The results showed the average length of tilapia fish P0 (9,45±1,06b); P1 (8,89±0,90a); P2 (8,86±0,87a) and P3 (8,66±0,85). Average weight of tilapia fish P0 (23,38±4,50); P1 (19,75±2,27); P2 (19,15±2,10) and P3 (18,65±2,00). Spesific growth rate P0 (38,7%); P1 (8,3%); P2 (4,3%) and P3 (3,3%). The rate of long-term growth of individual fish P0 (0,46 mm/d); P1 (0,27 mm/d); P2 (0,21 mm/d) and P3 (0,19 mm/d). In conclusion, it showed that the concentration of lead (Pb) and the duration of exposure has affect the growth rate of fish. The influential concentration of lead is 25,06 mg/L.
JUMLAH SEL GOBLET PADA USUS HALUS AYAM KAMPUNG (Galludomesticus) YANG TERINFEKSI Ascaridia galli SECARA ALAMI Ummu Balqis; M. Hanafiah; Connie Januari; M. Nur Salim; Siti Aisyah; Yudha Fahrimal
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i1.3001

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Penelitian ini bertujuan menghitung jumlah sel Goblet pada setiap 1000 sel absortif usus halus ayam kampung yang terinfeksi Ascaridia galli secara alami. Penelitian ini menggunakan 10 usus halus ayam kampung yang didapat dari pasar di Banda Aceh. Usus halus ayam kampung diukur kemudian dibagi menjadi tiga bagian (duodenum, jejunum, dan ileum). Kemudian masing-masing bagian usus dibelah dan dihitung jumlah cacing Ascaridia galli. Masing-masing bagian usus tersebut dipotong sepanjang 2 cm, lalu ditempelkan di kertas karton. Kemudian dibuat preparat histopatologis dengan pewarnaan hematoksilin dan eosin. Parameter dalam penelitian ini adalah jumlah sel Goblet pada setiap 1000 sel absortif duodenum, jejenum, dan ileum. Hasil penelitian menunjukkan bahwa jumlah sel Goblet pada setiap 1000 sel absorbtif usus halus yang terinfeksi Ascaridia galli dengan infeksi ringan, sedang, dan berat secara berturut-turut adalah 465, 480, dan 484. Berdasarkan hasil penelitian dapat disimpulkan bahwa semakin banyak jumlah infeksi Ascaridia galli di duodenum, jejenum, dan ileum maka semakin meningkat proliferasi sel Goblet.
Reduction of Religion: Attitude and Religious Behavior of Indonesian College Students M. Hanafiah; Nadiyah; Lutfi, Lutfi
Al-Banjari : Jurnal Ilmiah Ilmu-Ilmu Keislaman Vol. 22 No. 2 (2023)
Publisher : Pascasarjana UIN ANTASARI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18592/albanjari.v22i2.12345

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This research is motivated by the phenomenon of strengthening radicalism in the campus involving students and lecturers. The findings of several studies confirm an increasing trend of radicalism among students. The purpose of this research is to answer the main issue of the research which is to find out radicalism among public and religious college students. This research is field research, data and information obtained directly in the field, using quantitative methods. Data collection techniques through a closed questionnaire (closed questionnaire) using a Likert Scale. A number of universities in Java and Kalimantan were sampled in this study. This research found that radicalism among public and religious college students fluctuated with narrative responses at the level of soft radicalism. However, the narrative of radicalism is so strong in understanding, but tends to show a downward trend in attitudes and behavior. The idea of ​​applying Islamic law is still the main choice for most respondents, but it is inversely related to the response to the application of the caliphate. Prohibition of Hizbut Tahrir Indonesia (HTI) organization is effective in reducing the attitude and behavior of radicalism among students.
Revitalisasi Lahan Terlantar: Ihyā' al-Mawāt karya Syekh Muhammad Arshad Al-Banjari sebagai Model Ekonomi Pesantren di Masyarakat Banjar Abad ke-18 Sairazi, Abdul Hafiz; Jamalie, Zulfa; Sukarni, Sukarni; Hanafiah, M.
Syariah: Jurnal Hukum dan Pemikiran Vol 24 No 2 (2024)
Publisher : Universitas Islam Negeri Antasari Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18592/sjhp.v24i2.13135

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This study examines the concept of ihyā’ al-mawāt as implemented by Sheikh Muhammad Arshad al-Banjari in 18th-century Banjarese society, focusing on its socio-economic and legal significance. While ihyā’ al-mawāt—the revitalization of abandoned land—is well-documented in Islamic jurisprudence, its practical application by Sheikh Muhammad Arshad remains underexplored. Using a historical approach and a normative legal framework, the research investigates the methods employed in land cultivation, the establishment of communal agricultural systems, and their integration into pesantren institutions. The findings reveal that Sheikh Muhammad Arshad spearheaded land reclamation through the collective efforts of his community, transforming swampy and neglected areas into fertile agricultural land. This initiative not only enhanced the community’s economic welfare but also laid the groundwork for an agro-economic model within pesantren education. The study underscores the enduring relevance of Islamic legal principles in addressing societal challenges and fostering sustainable community development.
EFFECT OF Nigella sativa AS FEED ADDITIVE ON ORGANOLEPTIC, WATER, PROTEIN, AND CHOLESTEROL CONTENT OF BROILER CHICKEN MEAT WITH HEAT STRESS CONDITION Vanda, Henni; Nurliana, Nurliana; Sugito, Sugito; Ferasyi, Teuku Reza; Sari, Wahyu Eka; Hanafiah, M.; Akram, Saydul; Daulay, Dina Khairani; Paula, Wita
Jurnal Kedokteran Hewan Vol 18, No 2 (2024): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i2.37355

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This study aimed to determine the physical properties, organoleptic, proximate value, and cholesterol level of broiler meat given black cumin (Nigella sativa) as feed supplement and exposed to heat stress. This study used 15-day old chicken (DOC) broilers that were adapted for 14 days and treated on day 15 to day 28. This study used a completely randomised design (CRD) consisting of 5 treatment groups: P0 (commercial feed); P1 (commercial feed + heat stress 34-35 ); P2 (commercial feed + black cumin 4% + heat stress 34-35); P3 (commercial feed + black cumin 6% + heat stress 34-35 ); P4 (commercial feed + black cumin 8% + heat stress 34- 35 ). Feeding was given twice a day at 07.00 WIB and 17.00 WIB ad libitum. Heat stress was given from 10.00 - 15.00 WIB every day for 14 days. The chicken breast and thigh meat were collected on day 29 for analysis. The statistical analysis showed that feed supplement containing black cumin had no effect (P0.05) on the pH value, drip loss, texture, protein content, and structure of broiler meat given exposure to heat stress, but had effect (P0.05) on the organoleptic colour, smell and texture, and cholesterol level of broiler meat. Based on these results, it can be concluded that feeding with 4%, 6% and 8% black cumin did not change the pH, drip loss, texture value as well as protein and water content of broiler meat given heat stress, but produced better colour and aroma. The administration of 4% cumin can reduce cholesterol levels of broiler meat exposed to heat stress.
Study of Tissue Cyst Formation Time of Toxoplasma gondii in Mice Hanafiah, M.; Nurcahyo, Wisnu; S, Sumartono
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i2.3132

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The purpose of the research was to study a tissue cyst formation time Toxoplasma gondiiexperimentally. A number of 84 mice were divided randomly into four groups. Each group consisted of 21mice. The mice of the group I were infected with 101, II with 102 and III with 10 tachyzoites respectivelyintraperitoneally, whereas the group IV as a control (not infected with tachyzoites). All infected micewere treated with sulfadiazine, 15 mg/mouse per oral diluted in drinking water, for 5 days. On first untiltwenty first day after treatment one mouse of each group was necropsied. Liver, lymph, kidney, lung,heart, brain, or diaphragm muscle were then taken for histological preparations. Data on tissue cystformation time was analysed descriptively. The research revealed that innoculation with tachyzoites 103cyst could be found on day 14th after infection of liver, 102 cyst was found on the 6 day of liver, in day7th in heart and brain on day 10th of after infection, 103 cyst was found on day 4th inheart and brain in day 7thth in liver, day 6 after infection, while in the control dosage there is no formation similar to cyst found.Keywords: cyst, tissue, T. gondii, mice th1
Pengetahuan Dan Sikap Orang Banjar Mengenai Produk Farmasi Bersertifikasi Halal Akhmad Jamaluddin Fikri; Anwar Hafidzi; M. Hanafiah
Indonesian Journal of Islamic Jurisprudence, Economic and Legal Theory Vol. 1 No. 4 (2023): Islamic Law, Religious Court System, and Judicial Decisions in Indonesia
Publisher : Sharia Journal and Education Center Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62976/ijijel.v1i4.145

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Abstract This study discusses the knowledge and attitudes of the people of Banjarmasin about halal-certified pharmaceutical products. The halal status of pharmaceutical products is in the spotlight because the Product Halal Guarantee Act requires halal certification. In the field, many drugs are unclear about their halal status, perhaps due to a lack of knowledge and public attention to this matter. This research is a field study of the knowledge and attitudes of the Banajarmasin community, with a phenomenological approach that is analyzed qualitatively descriptive. Data collection techniques using interviews. The conclusion of this study is that the level of knowledge of the banjar people regarding halal certification is high but the attitude of the banjar people regarding halal certification is low because they do not pay attention to halal-certified products when buying a medicine. Keywords: Halal certificate, Knowledge, Attitude Abstrak Penelitian ini membahas tentang pengetahuan dan siakap masyarakat Banjarmasin tentang produk farmasi bersertifikasi halal. Status kehalalan produk farmasi menjadi sorotan karena Undang-Undang Jaminan Halal Produk mengharuskan sertifikasi halal. Di lapangan, banyak obat yang belum jelas kehalalannya, mungkin karena kurangnya pengetahuan dan perhatian masyarakat terhadap hal tersebut.. Penelitian ini bersifat kajian lapangan terhadap pengetahuan dan sikap masyarakat Banajarmasin, dengan pendekatan fenomenologi yang dianalisis secara kualitatif deskriptif. Teknik pengambilan data menggunakan wawancara. Kesimpulan penelitian ini adalah tingkat pengetahuan orang banjar mengenai sertifikasi halal terbilang tinggi tetapi sikap orang banjar mengenai sertifikasi halal terbilang rendah karena mereka ketika membeli suatu obat tidak memperhatikan produk yang bersertifikasi halalnya. Kata Kunci: Sertifikat halal, Pengetahuan, Sikap