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All Journal Klinikal Sains (Jurnal Analis Kesehatan) JURNAL KESEHATAN PERINTIS INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Medika Cendikia (e-Journal) Jurnal Ilmiah Farmako Bahari Science Midwifery Jurnal Sains dan Teknologi Laboratorium Medik Jurnal Kesehatan Jurnal Abdi Masyarakat Kita Jurnal Kesehatan Amanah Ekalaya: Jurnal Pengabdian Kepada Masyarakat Indonesia Mukhtabar: Journal of Medical Laboratory Technology Jurnal Riset Ilmu Kesehatan Umum dan Farmasi Jurnal Pengabdian Masyarakat dan Riset Pendidikan Journal of Innovative and Creativity Compromise Journal : Community Proffesional Jurnal Ilmiah Ilmu Kesehatan
Mamay
Department Of Medical Laboratory Technology, STIKes Karsa Husada, Garut, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Differences in Urine Protein Levels of Hemodialysis Patients With Acetic Acid and Lemon Juice Examination Nurani Ai Erlinawati; Mamay Mamay; Nita Wahyuni
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 2 (2023): Mukhtabar: Journal of Medical Laboratory Technology (October 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i2.384

Abstract

Background and Objectives: Urine protein examination is a routine examination performed to determine kidney function. The principle of urine protein examination is that the protein in urine is denatured by heating and adding acid. Lemon fruit has a high acid content and lemon has similar properties to acetic acid, namely properties as a weak acid with a pH of 3.0. The direction of this research is to understand the comparison of urine protein using lemon juice and acetic acid solution reagents. Methods: This research uses experimental techniques because urine is treated directly by testing using a 6% acetic acid solution (control) and lemon juice solution. Results: the examination results using lemon juice solution are the same as the examination results using 6% acetic acid solution (control), using the Wilcoxon test, the Asymp.sig (2-tailed) value is 1.000.  With the provisions of the test >0.05, Ho is accepted and Ha is rejected, so testing the difference in urine protein levels of hemodialysis patients with acetic acid and lemon juice examination can be carried out. Conclusion: The results of urine protein examination with acetic acid (control) and lemon juice as an alternative reagent. There is no difference in the results of urine protein examination using 6% acetic acid reagent and lemon juice.
PATOGENITAS BAKTERI HIDROLITIK YANG DI ISOLASI DARI LIMBAH CAIR BIOMEDIS DI RSUD DR. SLAMET Mamay Mamay; N. Ai Erlinawati; Zahara Farhan; Rina Herlina; Ranti Nurwantika; Elsa Zalfa Agustin
Jurnal Ilmiah Farmako Bahari Vol 14, No 2 (2023): Jurnal Ilmiah Farmako Bahari
Publisher : Faculty of Mathematic and Natural Science, Garut University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52434/jifb.v14i2.2433

Abstract

Bakteri patogen rendah sampai non patogen merupakan kriteria bakteri sebagai agen bioremediasi limbah cair biomedis. Bakteri indigen menghasilkan enzim pendegradasi komponen yang terkandung limbah biomedis cair seperti karbohidrat, protein dan lemak. Bakteri yang memetabolisme zat-zat organik dapat mengurangi berkembang biaknya mikroorganisme patogen. Selain itu, bioremediasi dengan bakteri indigen dengan bakteri patogenitas rendah dapat mengurangi bahaya kontaminasi dan infeksi dari mikroorganisme patogen. Tujuan penelitian ini mengetahui patogenitas bakteri hidrolitik yang diisolasi dari limbah cair biomedis di RSUD dr. Slamet Garut. Penelitian bersifat deskriptif menggunakan sampel limbah cair biomedis dari RSUD dr. Slamet Garut. Pengambilan sampel dilakukan dengan teknik Cluster Random Sampling pada penampungan, inlet dan outlet.  Isolasi bakteri protease dan lipase dengan media skrining susu skim agar dan rhodamin agar. Pengujian  patogenitas bakteri dengan menumbuhkan bakteri pada agar mac conkey, agar darah dan agar coklat. Pada media skrining  terisolasi bakteri penghasil protease sebanyak 89 koloni dan lipase sebanyak 76 koloni. Uji patogenitas menunjukan bakteri penghasil protease dengan patogenitas rendah 21,3%, sedang 27%, tinggi 36%, sangat tinggi 4,5%, ekstra tinggi 11,2%  dan bakteri penghasil lipase dengan patogenitas rendah 20%, sedang 45%, tinggi 30%, sangat tinggi 4% dan ekstra tinggi 1%. Dari penelitian diperoleh bakteri penghasil protease dan lipase dengan sebagian kecil memiliki patogenitas rendah sebagai kandidat agen bioremediasi.
Bekam Basah Berpengaruh terhadap Penurunan Kadar Kolesterol Total Muhammad Hadi Sulhan; Tantri Puspita Tantri Puspita; Mamay Mamay; Gina Nafsa Mutmainna Gina Nafsa Mutmainna
PrimA: Jurnal Ilmiah Ilmu Kesehatan Vol. 10 No. 1 (2024): PrimA: Jurnal Ilmiah Ilmu Kesehatan
Publisher : LPPM STIKES Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47506/zxyq5959

Abstract

Abstract Introduction: Wet cupping is known to significantly lower total cholesterol, low-density lipoprotein (LDL)/high-density lipoprotein (HDL) ratio. Objective: To determine the effect of wet cupping therapy on reducing total cholesterol levels. Method: The research method used is a quasiexperiment with purposive sampling. Results: The results showed a significant effect of wet cupping therapy on total cholesterol levels.. Conclusion: Wet cupping therapy affects the decrease in total cholesterol levels.
Gambaran Kadar C-Reactive Protein (CRP) Pada Mencit (Mus Musculus) Yang Diberi Minuman Kemasan Tinggi Gula Meti Rizki Utari; Astari Nurisani; Sugiah; Iwan Wahyudi; Muhammad Hadi Sulhan; Gina Nafsa Mutmaina; Mamay; Lia Mar’atiningsih; N. Ai Erlinawati
Journal of Innovative and Creativity Vol. 6 No. 1 (2026)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v6i1.7118

Abstract

Minuman ringan merupakan minuman olahan nonalkohol yang umumnya mengandung gula sederhana dan diduga berhubungan dengan peningkatan kadar C-Reactive Protein (CRP) sebagai penanda inflamasi. Penelitian ini bertujuan untuk mengetahui gambaran kadar CRP pada mencit (Mus musculus) yang diberi minuman kemasan tinggi gula. Penelitian ini merupakan penelitian eksperimental dengan desain posttest only control group yang menggunakan 24 ekor mencit berumur 3–4 bulan dengan berat badan 20–30 gram. Hewan uji dibagi ke dalam empat kelompok, terdiri atas satu kelompok kontrol yang diberikan aquadest dan tiga kelompok perlakuan yang masing-masing diberikan minuman kemasan tinggi gula dengan dosis 0,13 ml, 0,26 ml, dan 0,39 ml per hari selama 10 hari. Pada hari ke-11 dilakukan pengambilan serum dan pemeriksaan kadar CRP secara kualitatif menggunakan reagen kit CRP dengan pengamatan adanya atau tidak adanya aglutinasi. Hasil penelitian menunjukkan bahwa seluruh sampel pada keempat kelompok memiliki kadar CRP dalam batas normal, yang ditandai dengan tidak ditemukannya aglutinasi, sehingga seluruh mencit (100%) dikategorikan normal. Tidak ditemukan perbedaan gambaran kadar CRP antara kelompok kontrol dan kelompok perlakuan. Disimpulkan bahwa pemberian minuman kemasan tinggi gula dengan dosis 0,13–0,39 ml per hari selama 10 hari pada mencit Mus musculus tidak menyebabkan peningkatan kadar CRP yang dapat terdeteksi secara kualitatif.
Penanganan Nilai Kritis pada Pemeriksaan Ureum Astari Nurisani; Lia Mar’atiningsih; Muuhammad Hadi Sulhan; Gina Nafsa Mutmaina; Sugiah Sugiah; Mamay Mamay; Meti Rizki Utari; Arsylia Intan Zori Ichsani
Jurnal Kesehatan Amanah Vol. 9 No. 1 (2025): Jurnal Kesehatan Amanah
Publisher : Universitas Muhammadiyah Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57214/jka.v9i1.808

Abstract

Critical values ​​in an examination indicate a disorder that requires special and rapid action to improve patient safety. Urea examination is one of the laboratory parameters that functions as an indicator in assessing the level of kidney function. Urea levels are considered critical if the results exceed 100 mg/dl. This study aims to understand how to handle critical values ​​in urea examinations. This study describes a case in the field of clinical chemistry related to critical results obtained from urea examinations. The object of the case study used in this study was a serum sample, with data showing a critical urea level of 185 mg/dl. Based on the confirmation results, this figure is in accordance with the condition of patients who have kidney failure and will undergo hemodialysis therapy. The conclusion of this study shows that handling critical values ​​in urea examinations is carried out by identifying patient identity and ensuring good sample quality. In addition, the sample testing process must be carried out carefully and precisely, control results must be optimal, the reagents used must have the appropriate temperature and not expired, and the equipment must be properly maintained and calibrated. Finally, confirmation and reporting of results must be carried out accurately and on time.
Penyuluhan Kesehatan dan Pemeriksaan Kadar Asam Urat pada Masyarakat di Jungsereh Garut Lia Mar’atiningsih; Sugiah Sugiah; Muhammad Hadi Sulhan; Gina Nafsa Mutmaina; Mamay Mamay; Astari Nurisani; Meti Rizki Utari; N.Ai Erlinawati
Compromise Journal Community Proffesional Service Journal Vol. 2 No. 3 (2024): Compromise Journal: Community Professional Service Journal
Publisher : LPPM STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/compromisejournal.v2i3.307

Abstract

Hyperuricemia is defined as increased levels of uric acid in the blood, this can result in gout which is characterized by pain and redness in the joints. Currently, increased uric acid levels are often caused by excessive consumption of red meat including offal, being overweight, consuming foods high in cholesterol, and rarely exercising. The purpose of this counseling and examination of uric acid levels is to increase public knowledge about gout and gout screening by conducting uric acid examinations using POCT. The implementation method used is direct delivery of material related to gout, symptoms, and prevention including screening of uric acid examinations using the POCT method.
Gambaran Kadar Trigliserida Pada Mahasiswa Penikmat Seblak di Kampus Stikes Karsa Husada Garut Sugiah Sugiah; Aceng Ali Awaludin; Lia Mar’atiningsih; M. Hadi Sulhan; Gina Nafsa Mutmaina; Mamay Mamay; Astari Nurisani; Meti Rizki Utari; Dendi Leona
Jurnal Riset Ilmu Kesehatan Umum dan Farmasi (JRIKUF) Vol. 3 No. 1 (2025): Januari : Jurnal Riset Ilmu Kesehatan Umum dan Farmasi (JRIKUF)
Publisher : LPPM STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/jrikuf.v3i1.496

Abstract

Seblak is a typical food or culinary dish from the city of Bandung, West Java, derived from the words segak and nyegak which mean stinging with a composition consisting of chewy crackers sautéed in onion-spiced sauce, which is sometimes added with sausage, eggs, sliced ​​vegetables, meatballs, shrimp, squid. and others. This study aims to determine the description of triglyceride levels in seblak connoisseurs at the STIKes Karsa Husada Garut Campus. The research design used in this study was descriptive in nature, the GPO-PAP examination method with the measurement results in the form of triglyceride levels in mg/dl. This measurement scale is included in the ordinal measurement scale. The research was conducted at the Karsa Husada Garut STIKes Clinical Chemistry Laboratory in June 2023. Samples were obtained from venous blood of D3 Health Analyst students at STIKes Karsa Husada Garut. The definition of student in the Big Indonesian Dictionary (KBBI) is a student who studies at a university. Students' eating habits are determined by culture, lifestyle, beliefs and the environment in which the community lives. Increased triglyceride levels (hypertriglyceridemia) is one part of dyslipidemia which is the main factor causing coronary heart disease. Hypertriglyceridemia has a role in causing coronary heart disease. Based on the average results obtained, it can be concluded that the research results depict triglyceride levels in students of consuming seblak at STIKes Karsa Husada Garut campus, most of the respondents had normal triglyceride levels.
UTILIZATION OF 1% OF METHYLENE BLUE IN STAINING HISTOPATHOLOGICAL PREPARATIONS AT ANATOMIC PATHOLOGY LABORATORY Tri Rahmawati; Yadi Apriyadi; Mamay
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i2.1563

Abstract

Tissue staining using hematoxylin-eosin (HE) is a standard method of histopathological staining. The tissue staining is hampered when there is no hematoxylin reagent in laboratory. Therefore, other reagents are needed that can replace the use of hematoxylin. Methylene blue is a basic dyes that interact with cell nuclei which has a negative ionic charge of the tissue. It can be used as an alternative nuclei staining. This study aims to evaluate the use of 1% of methylene blue in cell nuclei staining in histopathological preparations. The research sample were 15 pathology preparations which were randomly selected including breast cancer, cervical cancer and ovarian cancer in the bank of sampel at anatomical pathology laboratory of RSUD Dr. Slamet Garut, Indonesia. The experiment showed that the methylene blue dyes yielded “worth” result (40%) and “poorly” result (60%). Further research can be carried out by modifying the pH of 1% of methylene blue reagent so that it can maximize the staining preparations results as good as those using hematoxylin.
Potential of Sappan Wood, Purple Cabbage and Beetroot Extract in Sperm Staining Mamay Mamay; Ernawati Ernawati; Astari Nurisani
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 2 (2023): Combatting Bacterial and Fungal Infections: The Critical Role of Advanced Researc
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i2.3961

Abstract

Examination of sperm morphology is an essential criterion for evaluating male fertility. This examination can be done by staining sperm cells with various techniques to facilitate the visualization of sperm cells. Several other methods that can be used to evaluate sperm morphology are Eosin-nigrosin, AgNO3 staining, Papanicolaou, Diff Quick and Giemsa. However, using these synthetic dyes can harm the environment and water sources because they prefer to decompose. The natural dye derived from environmentally friendly plants expected to minimize hazardous waste. This study aimed to identify and compare the potency of several natural dyes derived from purple cabbage, sappan wood and beetroot, which have not been studied in sperm studies. Sperm obtained from 30 men in the campus area and underwent 3-5 days of abstinence from intercourse in preparation for sampling. The sperm stained with natural dye, and their quality compared with WHO standard Papanicolaou. Extracts of sappan wood, purple cabbage and beetroot are made with various mordant alum, ethanol and acetic acid compositions. The study's results showed that the presence of mordant increased the staining quality of sperm with sappan wood and purple cabbage extracts. The use of acid improves the quality of sperm staining with beet extract. There was no different between dyeing with beetroot extract in various solvent compositions and dyeing with Papanicolaou to color all sperm components (p> 0.05). The results showed that beetroot extract has a high potential to evaluate sperm morphology.
Sinergi Sekolah dan Orang Tua dalam Pencegahan Dini Diabetes Anak Melalui Skrining Glukosa Dan Edukasi Pemanfaatan Magnesium-Daun Insulin Astari Nurisani; Lia Mar’atiningsih; Meti Rizki Utari; Gina Nafsa Mutmainah; Mamay; Muhammad Hadi Sulhan; Sugiah; N. Ai Erlinawati; Eli Laelawati
Jurnal Pengabdian Masyarakat dan Riset Pendidikan Vol. 4 No. 3 (2026): Jurnal Pengabdian Masyarakat dan Riset Pendidikan Volume 4 Nomor 3 (Januari 202
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jerkin.v4i3.5043

Abstract

Diabetes melitus (DM) kini menjadi tantangan kesehatan yang tidak hanya menyerang orang dewasa, tetapi juga mulai mengancam anak-anak akibat pola hidup tidak sehat dan kurangnya edukasi sejak dini. Kegiatan pengabdian masyarakat ini bertujuan untuk mencegah risiko diabetes pada anak melalui skrining glukosa darah sewaktu (GDS) serta edukasi pemanfaatan terapi herbal berbasis magnesium dan daun insulin (Smallanthus sonchifolius) kepada orang tua siswa. Sasaran kegiatan adalah 18 siswa (usia 4,5–7 tahun) dan 18 orang tua yang tergabung dalam Persatuan Orang Tua Murid dan Guru (POMG) di TK Islam Ar Rayyan Karangpawitan. Metode pelaksanaan mencakup koordinasi dengan pihak sekolah, pelaksanaan skrining glukosa pada anak dan orang tua, serta penyuluhan mengenai manajemen nutrisi preventif. Hasil skrining menunjukkan rata-rata kadar GDS anak sebesar 115 mg/dL (rentang 88–142 mg/dL) dan rata-rata GDS orang tua sebesar 102 mg/dL (rentang 77–141 mg/dL). Meskipun hampir seluruhnya subjek berada dalam kategori normal, temuan nilai ambang atas pada anak (142 mg/dL) menegaskan pentingnya pengawasan pola konsumsi gula. Sinergi antara sekolah dan POMG dalam kegiatan ini terbukti efektif dalam mendukung deteksi dini dan meningkatkan literasi kesehatan keluarga. Model kolaborasi ini berpotensi dikembangkan sebagai program promosi kesehatan berkelanjutan untuk memutus rantai risiko diabetes intergenerasional melalui pemanfaatan bahan alami yang aman dan mudah diterapkan di rumah.
Co-Authors Aceng Ali Awaludin Acep Gunawan Aldi Renaldi Septian Ana Hidayati Mukaromah Arsylia Intan Zori Ichsani Astari Nurisani Dendi Leona Diah Wardani Diah Wardani Eli Laelawati Elsa Zalfa Agustin Fathul Hakim Gina Nafsa Mutmaina Gina Nafsa Mutmaina Gina Nafsa Mutmainah Gina Nafsa Mutmainna Gina Nafsa Mutmainna Iin Patimah Iwan Wahyudi Lia Mar’atiningsih M. Hadi Sulhan Meti Rizki Utari Muhammad Hadi Sulhan Muuhammad Hadi Sulhan N Ai Erlinawati N.Ai Erlinawati Nita Wahyuni Nurani Ai Erlinawati Nurul Nurul Purwanto AP Raihan Nur Aulia Ranti Nurwantika Riki Ananda Rina Herlina Sugiah Sugiah Sugiah Tantri Puspita Tantri Puspita Titi Purwitasari Tri Rahmawati Wisnu Arya Pratama Yadi Apriyadi Zahara Farhan