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Angka Kuman Susu Formula Pasca Seduh pada Penyimpanan Suhu Lemari Pendingin dengan Variasi Lama Penyimpanan Mamay Mamay; Iin Patimah; Ernawati Ernawati; Aldi Renaldi Septian
Jurnal Kesehatan Vol. 11 No. 1 (2023): April : Health Journal "Love That Renews"
Publisher : Sekolah Tinggi Ilmu Kesehatan Santo Borromeus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55912/jks.v11i1.135

Abstract

One of the factors that influence the incidence of diarrhea in infants is formula feeding. Statistical results show that there is a relationship between formula feeding and the incidence of diarrhea in infants aged 0-6 months. Formula milk should be consumed immediately after brewing. Storage in the refrigerator can be done if formula milk is not consumed immediately to reduce the risk of microbial growth, one of which is bacteria. This study aims to calculate the number of germs or bacteria in post brewed formula milk in the refrigerator with several storage times, namely 0, 12, 24 and 36 hours. This research is an experimental study. The shelf life of 0 and 12 hours, the total germ count, which is 1.103, is still below the threshold for microbial counts and able for consumption. As for the 24-hour shelf life and 36-hour shelf life, the total has exceeded the microbial count threshold > 1.104 and is not good for consumption. Total microbes increased with the length of storage period at refrigerator temperature. It is expected not to consume post-boiled milk that is stored in the refrigerator for more than 12 hours.
Differences in Urine Protein Levels of Hemodialysis Patients With Acetic Acid and Lemon Juice Examination Nurani Ai Erlinawati; Mamay Mamay; Nita Wahyuni
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 2 (2023): Mukhtabar: Journal of Medical Laboratory Technology (October 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i2.384

Abstract

Background and Objectives: Urine protein examination is a routine examination performed to determine kidney function. The principle of urine protein examination is that the protein in urine is denatured by heating and adding acid. Lemon fruit has a high acid content and lemon has similar properties to acetic acid, namely properties as a weak acid with a pH of 3.0. The direction of this research is to understand the comparison of urine protein using lemon juice and acetic acid solution reagents. Methods: This research uses experimental techniques because urine is treated directly by testing using a 6% acetic acid solution (control) and lemon juice solution. Results: the examination results using lemon juice solution are the same as the examination results using 6% acetic acid solution (control), using the Wilcoxon test, the Asymp.sig (2-tailed) value is 1.000.  With the provisions of the test >0.05, Ho is accepted and Ha is rejected, so testing the difference in urine protein levels of hemodialysis patients with acetic acid and lemon juice examination can be carried out. Conclusion: The results of urine protein examination with acetic acid (control) and lemon juice as an alternative reagent. There is no difference in the results of urine protein examination using 6% acetic acid reagent and lemon juice.
PATOGENITAS BAKTERI HIDROLITIK YANG DI ISOLASI DARI LIMBAH CAIR BIOMEDIS DI RSUD DR. SLAMET Mamay Mamay; N. Ai Erlinawati; Zahara Farhan; Rina Herlina; Ranti Nurwantika; Elsa Zalfa Agustin
Jurnal Ilmiah Farmako Bahari Vol 14, No 2 (2023): Jurnal Ilmiah Farmako Bahari
Publisher : Faculty of Mathematic and Natural Science, Garut University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52434/jifb.v14i2.2433

Abstract

Bakteri patogen rendah sampai non patogen merupakan kriteria bakteri sebagai agen bioremediasi limbah cair biomedis. Bakteri indigen menghasilkan enzim pendegradasi komponen yang terkandung limbah biomedis cair seperti karbohidrat, protein dan lemak. Bakteri yang memetabolisme zat-zat organik dapat mengurangi berkembang biaknya mikroorganisme patogen. Selain itu, bioremediasi dengan bakteri indigen dengan bakteri patogenitas rendah dapat mengurangi bahaya kontaminasi dan infeksi dari mikroorganisme patogen. Tujuan penelitian ini mengetahui patogenitas bakteri hidrolitik yang diisolasi dari limbah cair biomedis di RSUD dr. Slamet Garut. Penelitian bersifat deskriptif menggunakan sampel limbah cair biomedis dari RSUD dr. Slamet Garut. Pengambilan sampel dilakukan dengan teknik Cluster Random Sampling pada penampungan, inlet dan outlet.  Isolasi bakteri protease dan lipase dengan media skrining susu skim agar dan rhodamin agar. Pengujian  patogenitas bakteri dengan menumbuhkan bakteri pada agar mac conkey, agar darah dan agar coklat. Pada media skrining  terisolasi bakteri penghasil protease sebanyak 89 koloni dan lipase sebanyak 76 koloni. Uji patogenitas menunjukan bakteri penghasil protease dengan patogenitas rendah 21,3%, sedang 27%, tinggi 36%, sangat tinggi 4,5%, ekstra tinggi 11,2%  dan bakteri penghasil lipase dengan patogenitas rendah 20%, sedang 45%, tinggi 30%, sangat tinggi 4% dan ekstra tinggi 1%. Dari penelitian diperoleh bakteri penghasil protease dan lipase dengan sebagian kecil memiliki patogenitas rendah sebagai kandidat agen bioremediasi.
Bekam Basah Berpengaruh terhadap Penurunan Kadar Kolesterol Total Muhammad Hadi Sulhan; Tantri Puspita Tantri Puspita; Mamay Mamay; Gina Nafsa Mutmainna Gina Nafsa Mutmainna
PrimA: Jurnal Ilmiah Ilmu Kesehatan Vol. 10 No. 1 (2024): PrimA: Jurnal Ilmiah Ilmu Kesehatan
Publisher : LPPM STIKES Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47506/zxyq5959

Abstract

Abstract Introduction: Wet cupping is known to significantly lower total cholesterol, low-density lipoprotein (LDL)/high-density lipoprotein (HDL) ratio. Objective: To determine the effect of wet cupping therapy on reducing total cholesterol levels. Method: The research method used is a quasiexperiment with purposive sampling. Results: The results showed a significant effect of wet cupping therapy on total cholesterol levels.. Conclusion: Wet cupping therapy affects the decrease in total cholesterol levels.
Gambaran Kadar C-Reactive Protein (CRP) Pada Mencit (Mus Musculus) Yang Diberi Minuman Kemasan Tinggi Gula Meti Rizki Utari; Astari Nurisani; Sugiah; Iwan Wahyudi; Muhammad Hadi Sulhan; Gina Nafsa Mutmaina; Mamay; Lia Mar’atiningsih; N. Ai Erlinawati
Journal of Innovative and Creativity Vol. 6 No. 1 (2026)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v6i1.7118

Abstract

Minuman ringan merupakan minuman olahan nonalkohol yang umumnya mengandung gula sederhana dan diduga berhubungan dengan peningkatan kadar C-Reactive Protein (CRP) sebagai penanda inflamasi. Penelitian ini bertujuan untuk mengetahui gambaran kadar CRP pada mencit (Mus musculus) yang diberi minuman kemasan tinggi gula. Penelitian ini merupakan penelitian eksperimental dengan desain posttest only control group yang menggunakan 24 ekor mencit berumur 3–4 bulan dengan berat badan 20–30 gram. Hewan uji dibagi ke dalam empat kelompok, terdiri atas satu kelompok kontrol yang diberikan aquadest dan tiga kelompok perlakuan yang masing-masing diberikan minuman kemasan tinggi gula dengan dosis 0,13 ml, 0,26 ml, dan 0,39 ml per hari selama 10 hari. Pada hari ke-11 dilakukan pengambilan serum dan pemeriksaan kadar CRP secara kualitatif menggunakan reagen kit CRP dengan pengamatan adanya atau tidak adanya aglutinasi. Hasil penelitian menunjukkan bahwa seluruh sampel pada keempat kelompok memiliki kadar CRP dalam batas normal, yang ditandai dengan tidak ditemukannya aglutinasi, sehingga seluruh mencit (100%) dikategorikan normal. Tidak ditemukan perbedaan gambaran kadar CRP antara kelompok kontrol dan kelompok perlakuan. Disimpulkan bahwa pemberian minuman kemasan tinggi gula dengan dosis 0,13–0,39 ml per hari selama 10 hari pada mencit Mus musculus tidak menyebabkan peningkatan kadar CRP yang dapat terdeteksi secara kualitatif.