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All Journal Jurnal Sain Veteriner Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences JURNAL BIOLOGI INDONESIA Jurnal Kedokteran Hewan
Muharam Saepulloh
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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STATUS INFEKSI VIRUS HENDRA PADA KALONG (PTEROPUS SPP.) DI PONTIANAK, KALIMANTAN BARAT DAN MANADO, SULAWESI UTARA Sendow, Indrawati; Field, Hume; Ratnawati, Atik; Adjid, RM. Abdul; Saepulloh, Muharam; Breed, Andrew; Morrissy, Chris.; Daniels, Peter
JURNAL BIOLOGI INDONESIA Vol 9, No 1 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i1.144

Abstract

Hendra merupakan salah satu penyakit emerging dan zoonosis yang berbahaya, termasuk Genus Henipavirus(Paramyxoviridae). Penyakit ini sangat erat hubungannya dengan Nipah, yang dapat menginfeksi ternak babi danmanusia. Survey serologi dilakukan di dua propinsi, yaitu Kalimantan Barat dan Sulawesi Utara. Hasil menunjukkanbahwa 148 kalong (Pteropus sp.) yang terdiri dari 84 P. vampyrus asal Kalimanatan Barat dan 64 P alecto asalSulawesi Utara telah dikoleksi. Hasil serologis mengindikasikan 22,6% P vampyrus di Kalimantan Barat mempunyaiantibodi terhadap virus Hendra, yang juga merupakan reaksi silang dengan virus Nipah. Di Sulawesi Utara, 25%serum mengandung antibodi terhadap virus Hendra, dimana 7,8% diantaranya hanya mempunyai antibodi terhadapvirus Hendra. Dari data tersebut dapat disimpulkan bahwa antibodi terhadap virus Hendra terdeteksi padaP. alecto di Sulawesi Utara. Hasil ini merupakan laporan pertama tentang infeksi Hendra pada P. alecto di Indonesia.Adanya perbedaan prevalensi tersebut, dapat disebabkan oleh letak geografis atau spesies kalong yang diuji.Kata kunci: P. vampyrus, P. alecto, antibodi, Hendra, Nipah, Serum Netralisasi
Bovine Viral Diarrhea Virus Antigen and Immunoglobulin-G Detection in Unvaccinated Cattle Suryohastari, Raden Rara Bhintarti; Saepulloh, Muharam; Jannah, Ana Roudlotul
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.101993

Abstract

To address the community's nutritional for meat can be supported by importing cattle while maintaining vigilance against Bovine Viral Diarrhea (BVD), an infectious disease caused by the Bovine Viral Diarrhea Virus (BVDV). This pathogen is associated with gastrointestinal, respiratory, and reproductive issues in cattle worldwide. Consequently, controlling BVDV stands as a paramount measure in preventing BVD. One effective strategy in this endeavor is vaccination. The successful implementation of vaccination relies on acquiring comprehensive information about BVDV antigens and antibodies present in the serum of unvaccinated cattle, which can provide insights into the protein profile resulting from natural infection. This study aimed to detect BVDV antigen and immunoglobulin-G (IgG) based on molecular weight in protein profiles from various cattle breeds imported into Indonesia. Utilizing the SDS-PAGE method, the analysis results revealed protein bands at 55.3 kDa and 151.3 kDa were detected in the serum of BVD-positive cows, meanwhile, these specific profiles were absent in BVD-negative cows. It can be concluded that the 55.3 kDa represents a BVDV antigen, while the 151.3 kDa corresponds to an IgG antibody profile. These findings can serve as one of the basic foundations for developing a BVD vaccine for cattle populations.
PEMETAAN GENETIK VIRUS RABIES PADA ANJING SEBAGAI DASAR PENETAPAN PENGENDALIAN PENYAKIT (Genetic Mapping of Rabies Virus in Dogs as a Basis for Disease Control) Saepulloh, Muharam; Adji, R. M. Abdul
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3369

Abstract

The purpose of this study was to determine the genetic characteristics of rabies virus based on phylogenetic relationship among rabies virus in various regions in Indonesia. The amino acid sequence of the nucleoprotein gene of rabies virus isolate from Banten (RV/Banten-01/dog/2007), Makasar (RV/MKS-26/dog/2010), Bukit Tinggi (RV/BKT-52/dog/2009 and RV/BKT-58/dog/2009), Medan (RV/Medan-27/dog/2007) and Bali (RV/Bali-1/dog/2009; RV/Bali-2/dog/2009; RV/Bali-3/dog/2009), Indonesia was determined. These isolates showed a high degree of homology among Indonesian isolates which reached 100%. Meanwhile, the level of homology between rabies virus isolates from cats rabies virus isolates from dogs reached 97%. Results of phylogenetic analysis using the amino acid sequences of the N genes showed that all of Indonesian rabies virus isolates were closely related to rabies viruses from China than those from Thailand, Laos, Burma, and Vietnam which geografically closer to Indonesia. Data obtained from the phylogenetic analysis is expected to trace the source of rabies spread and the possibility to create a vaccines which more suitable with rabies virus that spreads in Indonesia. Based on the phylogenetic relationship analysis using the amino acid sequence of the rabies virus N protein gene showed that all of rabies virus isolated from Indonesian regions share a high homology with others ranging from 97-100%.Key words: sequencing, rabies, nucleoprotein gene (N), homology
PENGEMBANGAN TEKNIK ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) MENGGUNAKAN ANTIBODI MONOKLONAL UNTUK MENDETEKSI ANTIBODI PENYAKIT BOVINE EPHEMERAL FEVER Sendow, Indrawati; Adjid, R.M. Abdul; Ratnawati, Atik; Saepulloh, Muharam
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2775

Abstract

Penelitian ini bertujuan mengembangkan teknik enzyme-linked immunosorbent assay (ELISA) untuk mendeteksi antibodi terhadap virus bovine ephemeral fever (BEF). Pada penelitian ini dikembangkan uji ELISA langsung (direct ELISA) dan tidak langsung (indirect ELISA) dengan menggunakan antibodi monoklonal (blocking ELISA). Hasil penelitian menunjukkan bahwa uji direct ELISA tidak dapat digunakan dengan baik karena terjadi positif palsu. Uji blocking ELISA bereaksi lebih baik dan dapat dikembangkan lebih lanjut untuk mendeteksi antibodi terhadap penyakit BEF. Dapat disimpulkan bahwa pengembangan teknik deteksi dini terhadap BEF dengan mempergunakan antibodi monoklonal dapat diterapkan dalam upaya pengawasan penyakit dan surveilans.
Co-Authors Adjid, RM. Abdul Adjid, RM. Abdul Assadah, Nur Sabiq Atik Ratnawati Breed, Andrew Breed, Andrew D. Sajuthi D. Setiyaningsih Daniels, Peter Daniels, Peter Darminto . Field, Hume Field, Hume Helmy Hamid Hope G Rovira I Wayan T. Wibawan I.W.T. Wibawan Indrawati Sendow Jannah, Ana Roudlotul Morrissy, Chris. Morrissy, Chris. NLP I Dharmayanti NLP Indi Dharmayanti R. M. Abdul Adji, R. M. Abdul R.M Abdul Adjid R.M. Abdul Adjid RISA INDRIANI Sendow, Indrawati Sjamsul Bahri Suryohastari, Raden Rara Bhintarti