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Journal : Jurnal Farmasi Sains dan Praktis

PROFIL KLT-BIOAUTOGRAFI DAN DENSITOMETRI SENYAWA TERAKTIF (ISOLAT KP13) DARI RIZOSFER KAYU PUTIH Alfian Syarifuddin; Nanik Sulistyani; Kintoko -
Jurnal Farmasi Sains dan Praktis Vol 5 No 1 (2019)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v5i1.2291

Abstract

The rate of infection, especially in Indonesia is still high. Treatment of infections by using antibiotics is constrained by cases of resistance to antibiotics so that it is necessary to explore microorganisms that can produce antibiotics. Antibiotics produced by KP13 bacterial isolates have the potential as antibiotics against Escherichia coli. The purpose of this study was to determine the TLC-Bioautography profile and Densitometry analysis of the most active fraction of KP13 bacterial isolates which have the potential as antibiotics against Escherichia coli bacteria. TLC using the mobile phase Chloroform: Ethylacetate: Methanol (4: 1: 0.5). Active compounds were analyzed using GC-MS. The results showed active spots during TLC-Biaoautography with a Rf value of 0.78. Compounds that are thought to be responsible for antibiotic activity detected by GC-MS at a Rf value of 0.78 have similarities with the compounds of cycloheptatriene and tetrahydrophyran..
OPTIMIZATION ANTIBACTERIAL PRODUCTION TIME OF ACTINOMYCETES ISOLATES (TE 235 ISOLATES) AGAINS ANTIBACTERIAL ACTIVITY ON ESCHERICHIA COLI AND STAPHYLOCOCCUS AUREUS Sutiara Prihatining Tyas; Alfian Syarifuddin; Ni Made Ayu Nila Septianingrum
Jurnal Farmasi Sains dan Praktis Vol 7 No 1 (2021)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v7i1.3547

Abstract

The search for new Antibiotics is needed in the hospital because of the multi-resistance Antibiotics that result in an increase in deaths due to Antibiotic abuse in dealing with infectious diseases, so it is necessary to explore to get potential new antibiotics. Actinomycetes are microorganisms that can produce is antibiotics. Actinomycetes isolate (isolate TE 235) has been isolated from rizosphere soil of sugarcane root crops. This study aimed to determine the optimal time of TE 235 isolates in producing secondary metabolites in the form of antibiotics that can test bacteria Escherichia coli and Staphylococcus aureus and the growth profile of TE 235 isolates were reviewed based on the production of cell biomass. The activity test method used in this study is a suitable test method. The results showed that the liquid culture of Actinomycetes isolates (isolate TE 235) had an optimal time to produce secondary metabolites (Antibiotics) against Staphylococcus aureus bacteria after incubation for ten days with inhibition zone diameter of 6.67 ± 0.94 mm, whereas against Escherichia coli bacteria on the seventh day with a diameter of inhibitory zone of 10.00 ± 0.82 mm. The results of research on the growth of Actinomycetes bacteria isolate TE 235 based on the weight of cell biomass that underwent the stationary phase on the 9th day. The incubation time of the culture of Isolate TE 235 to obtain optimal approval of antibiotics after ten days of incubation in terms of antibiotic activity on Staphylococcus aureus and Escherichia coli and the growth phase of TE235 isolates as the stationary phase.
SINERGISTIC COMBINATION OF ALOE VERA AND KERSEN LEAF EXTRACTS FOR INHIBITION OF THE GROWTH OF Staphylococcus aureus 25923 Nanik Sulistyani; Zikri Almahdi; Zainab -; Alfian Syarifuddin
Jurnal Farmasi Sains dan Praktis Vol 8 No 3 (September-December 2022)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v8i3.6268

Abstract

Aloe vera leaf (Aloe vera L.) and cherry leaf (Muntingia calabura L.) contain bioactive compounds that serve as the potential antibacterial agent. The combination treatment of multiple extracts can produce a stronger effect than a single compound. This study aims to determine the synergistic combination of aloe vera leaf extract (EDLB) and cherry leaf extract (EDK) as an antibacterial agent against Staphylococcus aureus ATCC 25923. The extract was obtained by maceration using 96% ethanol. Chemical content in the extract was analyzed by tube test and thin layer chromatography (TLC). Antibacterial activity against S. aureus ATCC 25923 was measured by the checkerboard microdilution method on a 96 well microplate. The effect of the combination of extracts on antibacterial activity was based on the fractional inhibitory concentration index (FICI) value. EDLB and EDK contain flavonoids, tannins, alkaloids, and saponins. The second combination showed a synergistic effect with a FICI value of 0.5 in inhibiting the growth of S. aureus with minimal inhibitory levels in a mixture of 0.625 mg/mL EDLB and 2.5 mg/mL EDK.