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All Journal HAYATI Journal of Biosciences Prosiding Seminar Nasional MIPA Biotika: Jurnal Ilmiah Biologi BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biogenesis: Jurnal Ilmiah Biologi AGRIVITA, Journal of Agricultural Science JURNAL ISTEK Journal of Tropical Biodiversity and Biotechnology Pro-Life REINWARDTIA BERITA BIOLOGI JURNAL BIOLOGI INDONESIA BIOEDUSCIENCE Jurnal Biologi Tropis Bioma : Jurnal Biologi Indonesia Makara Journal of Science Reinwardtia
Andi Salamah
Department Of Biology, Faculty Of Mathematic And Natural Sciences, Universitas Indonesia, Depok 16424, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Veterinary

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Journal : Makara Journal of Science

 1 
Induction of Callose Deposition in Tobacco (Nicotiana tabacum) by Bacterial Lipopolysaccharide Pseudomonas syringae pv. tabaci and Pseudomonas syringae pv. Glycinea Marianingsih, Pipit; Salamah, Andi; Ichinose, Yuki
Makara Journal of Science Vol. 18, No. 4
Publisher : UI Scholars Hub

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Lipopolysaccharide (LPS) is a major component of outer-membrane gram-negative bacteria, and it can act as a Pathogen-Associated Molecular Pattern (PAMP) for perception of pathogens by plants. LPS can be recognized by plants, triggering certain plant defense-related responses, including callose deposition. This study investigated induction of callose deposition by bacterial LPS in tobacco. Tobacco leaves were infiltrated with 400 µg/mL and 800 µg/mL LPS extracted from Pseudomonas syringae pv. tabaci (Pta) and Pseudomonas syringae pv. glycinea (Pgl) and incubated for 24 h or 48 h. To detect callose deposition, tobacco leaves were cleared in lactophenol solution, stained with aniline blue, and visualized by fluorescence microscopy. Results showed that LPS from Pgl induced more callose deposition in tobacco leaves than did that from Pta. In addition, a Pearson correlation test revealed that incubation period was the most significant factor in callose deposition, followed by the type of LPS bacteria. However, LPS concentration was not significantly corelated to callose deposition in tobacco leaves
Screening and Integration Analysis of OsDREB1A BC4F2 and BC5F1 Generations of Transgenic Ciherang Rice (Oryza sativa L.) for High-Salinity Tolerance Priyono, Dika Migi; Santoso, Tri Joko; Salamah, Andi
Makara Journal of Science Vol. 17, No. 2
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Salinity is one of a number of abiotic stresses that threaten rice production in Indonesia. To support food security programs, BB-Biogen has developed rice lines derived from OsDREB1A transformed Ciherang up to BC4F2 and BC5F1 generations. To verify the salinity tolerance and the stability of transgene integration, the BC4F2 and BC5F1 generations of Ciherang-OsDREB1A transgenic were screened to select high-salinity tolerant lines. Second, molecular analysis using the primers hptII-F/hptII-R and 35S-496-F/OsDREB1A-R was conducted to identify the existence and the stability of the transgene integration in the BC4F2 and BC5F1 generations. Screening 543 BC4F2 and BC5F1 Ciherang-OsDREB1A transgenic lines in a nutrient solution with a final electrical conductivity (EC) of approximately 18 mS/cm for 26 days yielded 134 putative transgenic plants. Integration analysis using the hptII-F/hptII-R primers showed that 73 of the 134 putative transgenic plants had positive PCR products, indicating the presence of the transgene in those plants. All the 73 plants also produced PCR products when tested with the specific primer 35S-496- F/OsDREB1A-R, indicating that transgene integration was maintained during the development of BC4F2 and BC5F1.
Morphological and Molecular Analysis and Flowering Time of T2 Generation Transgenic Rice cv. Nipponbare Carrying CONSTANS (CO) Gene Irshanty, Firdha Meidi; Salamah, Andi; Santoso, Tri Joko
Makara Journal of Science Vol. 8, No. 1
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Flowering is an important process that initiates plant productivity. Rice (Oryza sativa cv. Nipponbare) was genetically engineered by introducing a CONSTANS (CO) gene from Arabidopsis thaliana (AtCO) to improve rice productivity. The CO gene is a gene responsible for inducing early flowering. The aims of the experiment were to use morphological and molecular analysis to study the integration of the AtCO gene and its influence on agronomic traits in the T2 generation of Nipponbare transgenic AtCO rice. Morphological observations showed that agronomic traits tended to be better in the transgenic plants than in the wild type (controls), although the transgenic plants flowered later than the wild type. Polymerase Chain Reaction (PCR) analysis showed that 169 out of 227 transgenic rice plants showed hpt and CO gene integration.
Co-Authors Abinawanto Abinawanto Achmad Dinoto Agus Haryadi Anggita R Hafsari Anna Widyastuti Ariyanti Oetari Asep Sadili Asep Sadili Atria, Mega Az Zahra, Najwa Wafiyah Bayu Adjie Citra Karina Windarti Decky Indrawan Junaedi Decky Indrawan Junaedi Dee Dee Al Farishy Dian Hendrayanti Dika Migi Priyono Dwiranti, Astari Edi Mirmanto Edi Mirmanto Edi Mirmanto Edi Mirtanto Endang Sukara Fadilah, Nurrahmi Firdha Meidi Irshanty Francis Q Brearley Francis Q Brearly Iriani, Nur Annisa Irshanty, Firdha Meidi Isnaenisa Rachma Khoiriyah, Istatik Kusumadewi Sri Yulita KUSWATA KARTAWINATA Kuswata Kartawinata Lulut Dwi Sulistyaningsih Mansur Halik Marlina Ardiyani Maryanto, Andi Eko Mouleidi Dwi Putri Muhammad Mansur Muhammad Mansur Mutia Syadewi Najwa Wafiyah Az Zahra Niarsi Merry Hemelda Niarsi Merry Hemelda Niarsi Merry Hemelda Nur Laili Pipit Marianingsih Pipit Marianingsih Pipit Marianingsih Priyono, Dika Migi Putrika, Afiatry Rahmadina, Amara Ririn Oktarina Sarah Tsabituddinillah Sarjiya Antonius Siska Apriliani Sulistiani Sulistiani, Sulistiani TRI JOKO SANTOSO Tri Joko Santoso Tri Joko Santoso Tri Muji Ermayanti Wellyzar Sjamsuridzal WIBOWO MANGUNWARDOYO Windri Handayani Windri Handayani Windri Handayani Wita Wardani Wita Wardani Yuki Ichinose Yuki Ichinose Yuniati, Ratna