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Journal : BERITA BIOLOGI

VARIASI GENETIK Lactobacillus fermentum Beijerink ASAL SAYUR ASIN BERDASARKAN ANALISIS RFLP 16S-23S rDNA ISR, RAPD-PCR DAN ERIC-PCR [Genetic Variation of Lactobacillus fermentum Beijerink Origin Sayur Asin Based on RFLP 16S-23S rDNA ISR, RAPD-PCR and ERIC-PCR Analysis] Sulistiani, Sulistiani; Mangunwardoyo, Wibowo; Abinawanto, Abinawanto; Sukara, Endang; Dinoto, Achmad; Salamah, Andi
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3242.558 KB) | DOI: 10.14203/beritabiologi.v16i2.2772

Abstract

Molecular analysis of Lactobacillus fermentum isolates is essential to understand their genetic variation in relations to their roles in sayur asin fermentation process. Combination of three molecular techniques which is restriction fragment length polymorphism (RFLP) of 16S23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA (RAPD-PCR) and an enterobacterial repetitive intergenic consensus (ERIC-PCR) analysis were performed to discriminate 19 representative isolates of L. fermentum isolated from sayur asin. The result showed that L. fermentum strain D11 is distantly related to other isolates based on RFLP using HhaI restriction enzyme and RAPDPCR analyses. In addition, both of RAPD-PCR and ERIC-PCR successfully determined the genetic variation among L. fermentum strains by exhibiting distinct 4-8 bands (800-2080 bp) and 4-10 bands (280-3050 bp), respectively. A dendogram generated from UPGMA cluster analysis of both RAPD-PCR and ERIC-PCR data showed two distinct genotypic groups exist among L. fermentum isolated from sayur asin in Indonesia.
DIVERSITY OF NEPENTHES SPECIES IN NORTH SUMATRA PROVINCE Mansur Halik; Andi Salamah; Edi Mirmanto
BERITA BIOLOGI Vol 21, No 3 (2022): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i3.4389

Abstract

Sumatra is the second island after Borneo, which has the highest diversity of Nepenthes species, spread from the lowlands to mountain forests. There are 38 species of Nepenthes recorded on Sumatra Island, and 33 of them are endemic. The study was conducted in 2019 and 2022 to know the diversity of Nepenthes in North Sumatra Province. The results showed that 22 species of Nepenthes were recorded in North Sumatra Province, consisting of 12 highland species, 3 midland species and 7 lowland species. N. tobaica is a highland species with a very wide distribution, spread over eight regencies in North Sumatra with a very abundant population, while N. sumatrana, N. rigidifolia, N. jamban, N. lingulata, and N. naga have limited distribution with a very small population and are potentially extinct.