Introduction: The prevalence of electronic cigarette use is increasing; however, public understanding of their ingredients and associated health risks remains inconsistent. Persistent misconceptions contribute to inaccurate perceptions of product safety, especially regarding oral health. This study aimed to analyze oral health knowledge and associated factors among office workers who use electronic cigarettes. Methods: An analytical cross-sectional study was conducted among 100 office workers who used electronic cigarettes at a state-owned enterprise in Jakarta, Indonesia, between September and October 2025. Participants were recruited using purposive sampling. Data were collected using a structured questionnaire assessing respondents’ demographic characteristics and knowledge of electronic cigarette contents and their oral health effects. Data were analyzed using descriptive statistics, cross-tabulation, and the chi-square test. Results: Overall, 58% of respondents demonstrated good knowledge of oral health, whereas 42% had poor knowledge. High levels of awareness were observed regarding nicotine content (86%) and the addictive potential of electronic cigarettes (65%), while misconceptions persisted regarding carbon monoxide, tooth discoloration, and halitosis. Knowledge level was significantly associated with gender, with female respondents demonstrating higher knowledge than male respondents (p=0.014). No significant associations were observed between knowledge level and education level, smoking status, motivation for electronic cigarette use, or duration of use (all p>0.05). Conclusion: Although most electronic cigarette users demonstrated good overall oral health knowledge, important misconceptions remained regarding carbon monoxide production, tooth discoloration, and halitosis. These findings highlight the need for targeted oral health education focusing on specific misconceptions related to the oral health effects of electronic cigarettes.