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Analysis of Voltage Difference Characteristics of Albumin Membranes and Vitelin Membranes of Chicken Eggs through Diazinon Effects Wira Dian Jauharah; Didik Rahadi Santoso; Unggul P. Juswono
Natural B, Journal of Health and Environmental Sciences Vol 4, No 2 (2017)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.209 KB) | DOI: 10.21776/ub.natural-b.2017.004.02.2

Abstract

Cells are the most important structural and functional part of any organism. Cells are protected by cell membranes that envelop cells, and separate cells with their environment. The effectiveness of the work of a cell membrane can be disrupted for various reasons, one of which is pesticide contamination. Pesticides in cells can damage cell metabolism or cell membrane permeability. Therefore, this study aims to determine how big the effect of diazinon insecticides on the characteristics of different potential membrane vitelin and membrane albumin chicken egg cells. Measurements were made using the microelectrode connected with the picoscope 5000 series, then the data appears displayed through the PC. The results of membrane potential measurements showed that the greater the concentration of insecticide given, the potential value of chicken egg membrane increased. Potential Differential Values Membrane of eggs with different concentrations ranged in -145 mV - 249 mV on vitelin membrane, and -142,5 mV - 242,5 mV on albumin membrane 
Analysis of Potential Differential Characteristics of Albumin Membranes and Vitelin Membranes Quail Eggs (Cortunix cortunix) Due to Hydrogen Peroxide Fluid Ummu Hidayati Mardiyah; Didik Rahadi Santoso; Unggul Pundjung Juswono
Natural B, Journal of Health and Environmental Sciences Vol 4, No 1 (2017)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.934 KB) | DOI: 10.21776/ub.natural-b.2017.004.01.10

Abstract

The research of analysis the potential difference characteristic of albumin and vitelline membranes quail egg (Coturnix Coturnix) due to contaminated by hydrogen peroxide (H 2 O 2 ) was conducted to determine the effect of hydrogen peroxide (toxic) against the resistance of a cell. Hydrogen peroxide (H 2 O 2 ) is one group of reactive oxygen species (ROS) that can damage lipids, proteins and nucleic acids because they can diffuseion freely passed by bilayer membrane, so that the hydrogen peroxide concentration needs to be controlled. The resistance of a cell can be determined from changes in the value of the measured membrane potential. The method used in the research applied a microelectrode connected with unity gain differential amplifier and PicoScope. The measurement results show that there is a change the potential difference at the albumin membrane and vitellin membrane of quail egg due to contaminated hydrogen peroxide (H 2 O 2 ). The highest value of the membrane potential difference in the concentration 0 ppm at the albumin membrane is (-144 ± 2 mV) and the vitelline membrane is (-172 ± 3 mV), while the lowest value of the potential difference in the concentration 4000 ppm is (-8 ± 2 mV) at the albumin membrane and (-18 ± 1 mV) at the vitellin membrane.
Design of CO2 Gas Measuring System Arinto Yudi Ponco Wardoyo; Setyawan Purnomo Sakti; Didik Rahadi Santoso
Natural B, Journal of Health and Environmental Sciences Vol 1, No 1 (2011)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (62.105 KB) | DOI: 10.21776/ub.natural-b.2011.001.01.7

Abstract

Carbon dioxide (CO2) has been known well as a contributor gas for the global warming. Regarding the impact, the measurement of the CO2concentration in the air is very important for the assessment. This paper presents the design of a CO2 instrument built by using the components that are available in the market.  The instrument contains a CO2 sensor, an interfacing system, and a computer. The test showed that the instrument worked well. It could be used to measure a CO2 concentration on line and in real time. 
Electrical Impedance Spectroscopic Analysis on Whole Blood Cells to Correlate Severity Level of Ischemic Stroke Patients Ferhiyan Nabila; Chomsin Sulistya Widodo; Didik Rahadi Santoso
Jurnal Penelitian Pendidikan IPA Vol 9 No 11 (2023): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i11.5443

Abstract

Measuring the impedance value of biological materials in the form of blood samples has been widely used to determine a person's health status. Ischemic stroke patients in terms of impedance values and correlates with morphological tests on changes in red blood cells. The Electrical Impedance Spectroscopy (EIS) method is applied by providing a blood sample which is detected by electrodes and read using a BIA tool set so that the results are obtained on a laptop. The results obtained from the EIS test are in the form of Bode graphs, Bode phase graphs, Nyquist graphs, and impedance values. The EIS method uses a frequency of 100 Hz to 100 kHz by injecting a current of 10 μA. In this study, the impedance value obtained for normal people was 1058 Ω to 709 Ω, while for ischemic stroke patients with various levels of condition, it was from 517 Ω to 761 Ω. When counting the number of morphological cells in the patient's blood, changes were found ranging from the most cell changes to the least, namely with a value of 44% to 19%. The severity level of ischemic stroke patients obtained based on impedance tests and morphological tests includes the order P4, P5, P3, P2, P10, P21, P15, P19, P1, P6, P13, P18, P14, P17, P16, P20, P12, P11, P8, P7, P9.