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All Journal Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Agro Teknologi Tropika JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal AGRISEP: Kajian Masalah Sosial Ekonomi Pertanian dan Agribisnis

Yenny Sariasih

Staf Pengajar Fakultas Pertanian, Universitas Bengkulu

Author-ID : 373491

Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Medicine & Pharmacology

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Articles

Pengaruh Fungi Mikoriza Arbuskular Dalam Medium Zeolit Terhadap Pertumbuhan dan Intensitas Penyakit Bercak Daun Pada Bibit Kakao Sariasih, Yenny; Hadisutrisno, Bambang; Widada, Jaka
Jurnal Agro Teknologi Tropika Vol 1, No 1 (2012)
Publisher : Jurnal Agro Teknologi Tropika

Show Abstract | Download Original | Original Source | Check in Google Scholar

In Indonesia, cocoa (Theobromae cacao L.) is one of the nations third-largest contributor of foreign exchange, but the problem that arises on cocoa plantations in Indonesia is the difficulty of obtaining a healthy cacao seedlings in large numbers for the rejuvenation of cocoa cropping. One of methods to obtained healthy cacao seedlings with good performance in large numbers is application of arbuscular mycorrhizal fungi (AMF). This researches aims to observe the role of AMF mass production in the zeolite medium on the growth and leaf spot intensity of cocoa seedlings. The researches were conducted at the field condition in Sleman, Yogyakarta and Mycological Laboratory of the Faculty of Agriculture and the Laboratory of Phytochemistry, Faculty of Pharmacy, University of Gadjah Mada, Yogyakarta. The main ingredient of the study is AMF mass production in the zeolite medium. The observed variables were the growth of cocoa seedlings, the number of spores from each location, the cocoa seedlings height, leaf numbers, wet weight, dry weight and root length of seedlings of cocoa, symptom and intensity of leaf spot disease, detection of salicylic acid (SA) manually and TLC methods, and environmental factors which include: temperature, humidity and light intensity. The results indicated that the only real difference in the height and number of leaves, whereas other variables were not significantly different. This is because AMF spores from all locations are derived from the similarity of the two genera, namely Glomus sp., and Gigaspora sp., and was almost the same amount. Leaf spot disease symptoms appeared only a few of the cocoa seedlings, but more due to unfavorable environmental factors and conditions are weak pathogens. Plant defense responses had not been established because the salicylic acid content in leaves of cocoa seedlings at 12 weeks has not been detected.

FIRST REPORT OF Begomovirus INFECTION ON PAPAYA IN BENGKULU, INDONESIA Sutrawati, Mimi; Parwito, Parwito; Priyatiningsih; Zarkani, Agustin; Sipriyadi; Sariasih, Yenny; Ganefianti, Dwi Wahyuni
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 1 (2021): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12149-55

First report of Begomovirus infection on papaya in Bengkulu, Indonesia. A field survey was conducted during 2019, wefound a severe systemic yellow mosaic, striped green mosaic on leaves petiole, green spots on the fruit of papaya, leafmalformation, and stunting symptoms on three papaya cultivation area in Rejang Lebong, Kepahiang, Bengkulu Tengah, andSeluma, Bengkulu Province, Indonesia. A begomo-like virus was inferred to be the possible cause of the virus-disease-likesymptoms. The study aimed to identify the causal of those typical symptoms on papaya. PCR using universal primer fortranscriptional activator protein (TrAp) and replication-associated protein (Rep) gene of Begomovirus successfully amplifiedthe DNA fragments of 900 bp in all 10 detected samples, except for samples with leaf malformation and stunting symptoms. Itis indicating that those typical symptoms on papaya is associated with Begomovirus infection, while the causal of leafmalformation and stunting is unknown yet. This work is the first report of Begomovirus infected papaya in Indonesia. Severedisease incidence caused by this pathogen was observed on papaya plants in Bengkulu Province that was in the range of 42â€“100%. This finding is a precious information to be used for identification, and characterization the species of the virus,determining control strategies against the disease.

Comparison of Two Huanglongbing Detection Methods in Samples with Different Symptom Severity: Perbandingan Dua Metode Deteksi Huanglongbing pada Sampel dengan Tingkat Keparahan Gejala yang Berbeda Sariasih, Yenny; Subandiyah, Siti; Widyaningsih, Sri; Khurshid, Tahir; Mo, Jianhua; Donovan, Nerida
Jurnal Fitopatologi Indonesia Vol. 20 No. 4 (2024): Juli 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.4.174-186

Perbandingan Dua Metode Deteksi Huanglongbing pada Sampel dengan Tingkat Keparahan Gejala yang Berbeda Huanglongbing atau citrus greening di Asia disebabkan oleh patogen Candidatus Liberibacter asiaticus (CLas) merupakan salah satu penyakit jeruk yang paling menghancurkan di seluruh dunia. Penyakit ini menjadi salah satu penyebab penurunan produksi jeruk di Indonesia. Gejala huanglongbing pada tanaman jeruk di lapangan dan rumah kaca ternyata memiliki tingkat keparahan yang berbeda. Penelitian ini dilakukan untuk mendeteksi CLas pada beberapa jenis jeruk berdasarkan gejala pada daun menggunakan PCR konvensional dan real-time (qPCR). Tiga pasang primer digunakan dalam penelitian ini, sepasang Las606/LSS untuk PCR konvensional dan dua pasang untuk qPCR, yaitu Las931/LSS dan Lj900F/Lj900R. Hasil penelitian menunjukkan bahwa blotchy mottle adalah gejala huanglongbing yang paling mudah dideteksi dan ditemukan di kebun dan rumah kaca. Pasangan primer Lj900F/Lj900R lebih cocok untuk mendeteksi patogen CLas menggunakan qPCR daripada Las606/LSS berdasarkan kurva leleh dan Ct value yang muncul. Deteksi qPCR lebih akurat dan sensitif bahkan dengan konsentrasi DNA yang lebih sedikit. Batas bawah Ct value sampel daun sehat ialah 34.08. Daun jeruk dinyatakan positif jika Ct value kurang dari 34.08. Ct value berdasarkan tingkat keparahan atau dengan skoring antara HLB daun simtomatik dari lapangan dan rumah kaca menunjukkan perbedaan yang cukup besar, yaitu Ct value sampel simtomatik dari lapangan lebih rendah daripada sampel rumah kaca.

PENGEMBANGAN BUDIDAYA JAMUR TIRAM PUTIH SEBAGAI AGRIBISNIS PROSPEKTIF BAGI GAPOKTAN SEROJA I KANDANG LIMUN BENGKULU Sariasih, Yenny
Jurnal AGRISEP JURNAL AGRISEP VOL 12 NO 01 2013 (MARCH)
Publisher : Badan Penerbitan Fakultas Pertanian, Universitas Bengkulu

This activity aims to foster the farmers who are members of Gapoktan Seroja I Kandang Limun, Bengkulu to initiate oyster mushroom cultivationas one of the prospective agribusinesses in Bengkulu. This business was chosen due to the fungus growing medium are available in huge quantities and being waste around Kandang Limun. Further, Oyster mushroom demand and price are high. This event is a program of Ipteks bagi Masyarakat (IBM) in collaboration with the Lembaga Pengabdian Masyarakat (LPM). Training activities include the cultivation, manufacture kumbung measuring 3x4m which capacity of 2000 baglogs, baglog manufacture, sterilization baglog, inoculation, incubation for 3-6 weeks until the harvesting and marketing. Gapoktan Seroja I have been able to make ± 1500 baglog and have been able to produce white oyster mushroom ± 5 to10 kg per day. Fresh mushrooms sold at Rp. 20,000- 24.000/kg. With good management and sustainable production, the oyster mushroom cultivation can be one of the prospective agribusiness for Gapoktan Seroja I Kandang Limun, Bengkulu.

ANALISIS IMPLEMENTASI PP NO. 12 TAHUN 2012 TENTANG INSENTIF PERLINDUNGAN LAHAN PERTANIAN PANGAN BERKELANJUTAN DI KAWASAN DANAU DUSUN BESAR KOTA BENGKULU Alimansyah, Alimansyah; Sariasih, Yenny; Yulianti, Yulianti
Jurnal AGRISEP JURNAL AGRISEP VOL 14 NO 01 2015 (MARCH)
Publisher : Badan Penerbitan Fakultas Pertanian, Universitas Bengkulu

This study aims to describe how the implementation of incentives as stipulated in Government Regulation No. 12 of 2012 concerning the protection of agricultural land incentives sustainable food in the Dusun Besar Lake area of the city of Bengkulu. This research is a descriptive study with a qualitative approach. Informants determination use purposive sampling. Data collection techniques consist of observation, interviews and discussions as well as documentation. Data analysis are conducted by inductively starting from data collection, data reduction, data presentation and data verification. Based on the results of a couple of aspects studied, namely communication, resources, disposition, and bureaucratic structures, further seen from the form of the provision of incentives in the form of : agricultural infrastructure development has not been run, the financing of research and development of improved varieties of seeds and no ease of access to information and existing technology (extension), the provision of production facilities, guarantee certificate issuance of land rights on agricultural land are not sustainable food and / or rewards for high achievers existing farmers, government programs. It was concluded that the implementation of the Regulation no 12tahun 2012 in incentives for farmers land owners in the Dusun Besar Lake area of the city of Bengkulu is not already running. While the constraints faced by the lack of regulations governing the area of land protection sustainable food. Therefore, intensive provision for sustainable food future land owners should be budgeted and city government programs through agencies or institutions concerned.

Estimation of Variance, Number of Controlling Genes Group and Heritability of Tomato Yield at the Low Altitude Eka Saputra, Helfi; Wahyuni Ganefianti, Dwi; Salamah, Umi; Sariasih, Yenny; Dwi Ardiansyah, Nico
Jurnal Hortikultura Indonesia (JHI) Vol. 10 No. 2 (2019): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Persilangan merupakan salah satu teknik untuk memperluas keragaman genetik. Tujuan penelitian adalah untuk mengestimasi keragaman genetik, heritabilitas dan jumlah kelompok gen pengendali karakter hasil dan komponen hasil tomat pada populasi F2 persilangan TMU-1 x TMU-2 di dataran rendah. Penelitian dilaksanakan di Kelurahan Kandang Limun, Kota Bengkulu dengan ketinggian 10 m dpl (dataran rendah) dari bulan April sampai Oktober 2016. Estimasi keragaman genetik, heritabilitas dan jumlah kelompok gen pengendali karakter ditentukan berdasarkan nilai tengah dan ragam pada populasi. Hasil penelitian menunjukkan semua karakter yang diuji memiliki jumlah kelompok gen pengendali sebanyak satu gen. Karakter yang dikendalikan oleh satu gen diharapkan fiksasi gen-gen akan cepat tercapai pada beberapa siklus awal seleksi. Delapan karakter memiliki heritabilitas tinggi. Masing-masing karakter dan heritabilitasnya adalah umur berbunga (52.90%), jumlah buah per tanaman (92.99%), panjang buah (77.10%), diameter buah (71.76%), jumlah rongga buah (85.68%), tebal daging buah (73.05%), padatan total terlarut (89.77%) dan bobot buah per tanaman (65.19%). Kata kunci: dataran rendah, heritabilitas, jumlah kelompok gen, keragaman, komponen hasil

Co-Authors Agustin Zarkani Alimansyah, Alimansyah Bambang HADISUTRISNO Donovan, Nerida Dwi Ardiansyah, Nico Dwi Wahyuni Ganefianti, Dwi Wahyuni Eka Saputra, Helfi JAKA WIDADA Khurshid, Tahir Mimi Sutrawati Mo, Jianhua Parwito Parwito, Parwito Priyatiningsih Sipriyadi Siti Subandiyah Sri Widyaningsih Umi Salamah Wahyuni Ganefianti, Dwi Yulianti Yulianti

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