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All Journal Jurnal Fitopatologi Indonesia Jurnal Agro Teknologi Tropika JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Yenny Sariasih
Staf Pengajar Fakultas Pertanian, Universitas Bengkulu
Agriculture, Biological Sciences & Forestry Medicine & Pharmacology

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Pengaruh Fungi Mikoriza Arbuskular Dalam Medium Zeolit Terhadap Pertumbuhan dan Intensitas Penyakit Bercak Daun Pada Bibit Kakao Sariasih, Yenny; Hadisutrisno, Bambang; Widada, Jaka
Jurnal Agro Teknologi Tropika Vol 1, No 1 (2012)
Publisher : Jurnal Agro Teknologi Tropika

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Abstract

In Indonesia, cocoa (Theobromae cacao L.) is one of the nations third-largest contributor of foreign exchange, but the problem that arises on cocoa plantations in Indonesia is the difficulty of obtaining a healthy cacao seedlings in large numbers for the rejuvenation of cocoa cropping. One of methods to obtained healthy cacao seedlings with good performance in large numbers is application of arbuscular mycorrhizal fungi (AMF). This researches aims to observe the role of AMF mass production in the zeolite medium on the growth and leaf spot intensity of cocoa seedlings. The researches were conducted at the field condition in Sleman, Yogyakarta and Mycological Laboratory of the Faculty of Agriculture and the Laboratory of Phytochemistry, Faculty of Pharmacy, University of Gadjah Mada, Yogyakarta. The main ingredient of the study is AMF mass production in the zeolite medium. The observed variables were the growth of cocoa seedlings, the number of spores from each location, the cocoa seedlings height, leaf numbers, wet weight, dry weight and root length of seedlings of cocoa, symptom and intensity of leaf spot disease, detection of salicylic acid (SA) manually and TLC methods, and environmental factors which include: temperature, humidity and light intensity. The results indicated that the only real difference in the height and number of leaves, whereas other variables were not significantly different. This is because AMF spores from all locations are derived from the similarity of the two genera, namely Glomus sp., and Gigaspora sp., and was almost the same amount. Leaf spot disease symptoms appeared only a few of the cocoa seedlings, but more due to unfavorable environmental factors and conditions are weak pathogens. Plant defense responses had not been established because the salicylic acid content in leaves of cocoa seedlings at 12 weeks has not been detected.
FIRST REPORT OF Begomovirus INFECTION ON PAPAYA IN BENGKULU, INDONESIA Sutrawati, Mimi; Parwito, Parwito; Priyatiningsih; Zarkani, Agustin; Sipriyadi; Sariasih, Yenny; Ganefianti, Dwi Wahyuni
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 1 (2021): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12149-55

Abstract

First report of Begomovirus infection on papaya in Bengkulu, Indonesia. A field survey was conducted during 2019, wefound a severe systemic yellow mosaic, striped green mosaic on leaves petiole, green spots on the fruit of papaya, leafmalformation, and stunting symptoms on three papaya cultivation area in Rejang Lebong, Kepahiang, Bengkulu Tengah, andSeluma, Bengkulu Province, Indonesia. A begomo-like virus was inferred to be the possible cause of the virus-disease-likesymptoms. The study aimed to identify the causal of those typical symptoms on papaya. PCR using universal primer fortranscriptional activator protein (TrAp) and replication-associated protein (Rep) gene of Begomovirus successfully amplifiedthe DNA fragments of 900 bp in all 10 detected samples, except for samples with leaf malformation and stunting symptoms. Itis indicating that those typical symptoms on papaya is associated with Begomovirus infection, while the causal of leafmalformation and stunting is unknown yet. This work is the first report of Begomovirus infected papaya in Indonesia. Severedisease incidence caused by this pathogen was observed on papaya plants in Bengkulu Province that was in the range of 42–100%. This finding is a precious information to be used for identification, and characterization the species of the virus,determining control strategies against the disease.
Comparison of Two Huanglongbing Detection Methods in Samples with Different Symptom Severity: Perbandingan Dua Metode Deteksi Huanglongbing pada Sampel dengan Tingkat Keparahan Gejala yang Berbeda Sariasih, Yenny; Subandiyah, Siti; Widyaningsih, Sri; Khurshid, Tahir; Mo, Jianhua; Donovan, Nerida
Jurnal Fitopatologi Indonesia Vol 20 No 4 (2024): Juli 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.4.174-186

Abstract

Huanglongbing or citrus greening in Asia caused by the pathogen Candidatus Liberibacter asiaticus (CLas) is one of the most devastating citrus diseases worldwide. This disease is one of the causes of decreased citrus production in Indonesia. Symptoms of huanglongbing in citrus plants in the field and greenhouses have different levels of severity. This study was conducted to detect CLas in several types of samples based on leaf symptoms using conventional and real-time PCR (qPCR). Three pairs of primers were used in this study, a pair of Las606/LSS for conventional PCR and two pairs for qPCR, namely Las931/LSS and Lj900F/Lj900R. The results showed that blotchy mottle is the most easily detected symptom of huanglongbing and found in fields and greenhouses. The Lj900F/Lj900R primer pair is more suitable for detecting CLas pathogens using qPCR than Las606/LSS based on the melting curve and Ct value that appear. qPCR detection is more accurate and sensitive even with lower DNA concentrations. The lower limit of Ct value of healthy leaf samples is 34.08. Citrus leaves are considered positive if the Ct value is less than 34.08. Ct value based on severity or scoring between HLB symptomatic leaves from the field and greenhouse showed a significant difference, i.e. the Ct value of symptomatic samples from the field was lower than that of greenhouse samples.
Co-Authors Agustin Zarkani Bambang HADISUTRISNO Donovan, Nerida Dwi Wahyuni Ganefianti, Dwi Wahyuni JAKA WIDADA Khurshid, Tahir Mimi Sutrawati Mo, Jianhua Parwito Parwito, Parwito Priyatiningsih Sipriyadi Siti Subandiyah Sri Widyaningsih