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Indeks Formula Eritrosit Untuk Uji Skrining Talasemia Beta Minor Doni Setiawan; Hendri Setiawan; Ary Nurmalasari
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 2 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i2.247

Erythrocyte indices have been used as the first indicator in screening for beta-thalassemia minor. However, iron deficiency in hemoglobinopathies can reduce the sensitivity of screening, and the spectrum of mutations of different hemoglobinopathies between populations will result in discontinuation of the results of different erythrocyte indices. The research objective in this study was to determine the description of the erythrocyte indices used to screen for beta-thalassemia trait in Ciamis. The research method used in this study was a quantitative descriptive, using a cross-sectional study, data collection had been done during March-May 2021. The subjects of this study were 104 parents of a beta-thalassemia trait who have children with a beta-thalassemia major in Ciamis. The examination parameters used were complete blood count, with processing twenty-six erythrocyte formula index values. Ninety-two respondents were included in the inclusion criteria. The results of the erythrocyte indices in beta-thalassemia trait are England and Fraser, RBC, Mentzer, Srivastava, Shine and Lal, Ricerca, Green and King, Das Gupta, Jayabose, Telmissani-MCHD, Telmissani-MDH, Huber-Herklotz, Kerman I, Kerman II, Sirdah, Ehsani, Keikhaei, Nishad, Wongprachum, Sehgal, Pornprasert, Sirachainan, Bordbar, Matos and Carvalho and CRUISE has cut-off value in that range according to previous studies. Only the Bessman erythrocyte indices has a value of 17, exceeding the cut-off. Twenty-five erythrocyte indices with cut-off value according to previous studies can be used for beta-thalassemia screening in Ciamis, except for the Bessman erythrocyte indices.

Skrining Talasemia Beta Minor pada Pelajar SMK di Kecamatan Ciamis Doni Setiawan; Atun Farihatun; Ary Nurmalasari
Jurnal Kesehatan Vokasional Vol 5, No 3 (2020): Agustus
Publisher : Sekolah Vokasi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jkesvo.57621

Latar Belakang: Frekuensi talasemia beta minor di Indonesia mencapai 6–10%. Talasemia beta minor bersifat asimtomatik sehingga peran deteksi dini perlu dilakukan untuk mencegah kelahiran talasemia mayor. Pemeriksaan hematologi telah diketahui dan teruji dapat digunakan untuk skrining talasemia beta minor pada populasi beresiko. Meningkatnya penderita talasemia beta mayor di Kabupaten Ciamis dikarenakan belum maksimalnya skrining dilakukan. Tujuan: Mengetahui frekuensi talasemia beta minor pada pelajar SMK di Kecamatan Ciamis. Metode: Penelitian ini menggunakan metode deskriptif kuantitatif, dengan teknik cross‑sectional, yang telah dilakukan sejak bulan Maret‑Juni 2020. Subjek penelitian adalah pelajar SMK di Kecamatan Ciamis. Parameter pemeriksaan yang digunakan untuk skrining talasemia beta minor adalah MCV, Indeks Men3er, Indeks RDW, dan OTOFT. Hasil: Dari seluruh pelajar yang mengisi kuesioner sebanyak 238 pelajar, hanya 60 pelajar yang bersedia dan memenuhi kriteria penelitian. Hasil pemeriksaan parameter skrining talasemia beta minor didapatkan 5 (8,3%) pelajar MCV < 80 fL, 3 (5%) pelajar Indeks Men8er <13, Indeks RDW < 220 6 (10%) pelajar dan 9 (15%) pelajar dengan OTOFT positif. Kesimpulan: Pada penelitian ini ditemukan 3 (5%) pelajar sebagai talasemia beta minor.

EDUKASI PENYAKIT THALASEMIA PADA MAHASISWA STIKES MUHAMMADIYAH CIAMIS Doni Setiawan; Henri Setiawan; Ary Nurmalasari
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 6, No 3 (2022): September
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v6i3.9655

ABSTRAKPrevalensi penyakit thalasemia di Indonesia, seperti di Kabupaten Ciamis mengalami peningkatan dalam kurun waktu lima tahun terakhir. Prinsip utama pencegahan munculnya penderita thalasemia baru adalah dengan mencegah kelahiran bayi thalasemia. Program yang efektif untuk menanggulangi masalah tersebut adalah dengan pendidikan publik penyakit thalasemia. Kegiatan program yang dapat menjadi solusi bagi permasalahan tersebut adalah kegiatan edukasi penyakit thalassemia pada mahasiswa. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pemahaman mahasiswa tentang penyakit thalasemia sehingga dapat mendukung program pemerintah untuk menurunkan angka kejadian kelahiran thalasemia. Metode kegiatan ini dilakukan dengan sosialisasi penyakit thalassemia yang diikuti oleh 82 mahasiswa STIKes Muhammadiyah Ciamis, Jawa Barat yang dilakukan secara daring. Tahapan kegiatan meliputi pembuatan buku digital thalasemia, penyebaran informasi kegiatan, pengerjaan pre test, sesi pemaparan materi, dan sesi evaluasi. Hasil kegiatan pengabdian masyarakat ini memperlihatkan kenaikan nilai rerata dan median post test yaitu 34% dan 50%. Hal ini menunjukkan setelah kegiatan ini terjadi peningkatan pemahaman mahasiswa terhadap penyakit thalasemia. Intervensi berupa edukasi dengan media buku digital dapat meningkatkan pemahaman terhadap penyakit thalasemia. Kata kunci: edukasi; thalasemia; buku digital; pelajar ABSTRACTThe increasing prevalence of thalassemia in Indonesia needs to be alerted. The main principle of preventing the emergence of new thalassemia sufferers is to prevent the birth of thalassemic babies. Effective programs to reduce the prevalence are public education, career screening, counseling, and information about the diagnosis. This community service activity aims to increase student understanding regarding thalassemia disease so that it can support government programs in reducing the incidence of thalassemia births. This community service was attended by 82 students of STIKes Muhammadiyah Ciamis, West Java. This community service was conducted online. There are several stages carried out in this activity, namely the creation of a thalassemia digital book, dissemination of activity information, pre-test work, material presentation sessions, and evaluation sessions. The results of this community service activity raised the average and median scores for the post-test, namely 66.97 and 80. This shows that after these activities there was an increase in students' understanding of thalassemia disease. Interventions in the form of education with ebook media can increase understanding of thalassemia disease. Keywords: education; thalassemia; ebook; student

Almond as Alternative Media for Growth of Staphylococcus aureus and Escherichia coli Ary Nurmalasari; Leni Marlina; Undang Ruhimat; Rahayu Nirmatul Mutmainah
JURNAL KESEHATAN STIKes MUHAMMADIYAH CIAMIS Vol. 9 No. 2 (2022): Jurnal Kesehatan (Oktober 2022)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jurkes.v9i2.344

The high cost of media for microbial culture encourages researchers to create alternative media using local raw materials at low prices. One of the most important media compositions for bacterial growth is protein. This content can be obtained from nuts, one of which is almonds (Prunus dulcis). The purpose of this study is to find natural media that has the potential for the growth of microorganisms at a low price. The research method used is an experimental method to determine the growth of Escherichia coli and Staphylococcus aureus bacteria on natural media of almonds. The results showed the growth of Escherichia coli and Staphylococcus aureus bacteria in this alternative media of almonds. Based on the results of the study, it can be concluded that almond nut media can be applied as an alternative medium in laboratory research, especially in the field of_microbiology. It is suggested that further research should be carried out on natural media which are sources of carbohydrates and proteins as alternative growth media for bacteria.

Overview of Anti Streptolysin O Test Results in Postpartum Fever Mothers in the Ciamis Region Ary Nurmalasari; Rohaidah Rohaidah; Doni Setiawan
Nurul Ilmi : Journal of Health Sciences and Midwifery Vol. 1 No. 2 (2023): Nurul Ilmi: Journal of Health Sciences and Midwifery (September 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/nuri.v1i2.366

Introduction: Puerperal fever is a symptom of postpartum infection. Symptoms of fever during puerperium often occur due to infection with group A Streptococcus hemoliticus bacteria. Objective: The purpose of this study was to determine the description of the results of anti-streptolysin o examination in mothers for the diagnosis of fever in the puerperium. Method: This study is a descriptive study conducted from January to May 2023 with 40 respondents who were in the postpartum period in the Ciamis Health Center working area. The instrument used was the slide method ASTO examination. The data collection technique was based on Accidental sampling which was then processed and presented descriptively in tabular form and explained in narrative. Result: The results of the Anti Streptolysin O examination of 40 respondents showed positive results as many as 2 people (5%) and 38 people (95%) with negative results. Conclusion: It can be concluded that mothers who are in the postpartum period in the Ciamis Health Center working area have negative Anti Streptolysin O average results.

Red Dragon Fruit Peel Extract (Hylocereus lemairei) as an Alternative Dye in Worm Egg Staining Ary Nurmalasari; Dinda Ayunastiti; Atun Farihatun; Rahayu Nirmatul M
JURNAL KESEHATAN STIKes MUHAMMADIYAH CIAMIS Vol. 10 No. 2 (2023): Jurnal Kesehatan (Oktober 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jurkes.v10i2.333

Helminthiasis is still a problem in Indonesia, and worm infections often occur without symptoms, so the disease receives less attention. Identification can be done by several methods, one of which is by direct examination using 2% eosin dye. However, 2% eosin dye is included in synthetic dyes that can harm ul to health. The alternative used is to utilize natural ingredients. Red dragon fruit Hylocereus lemairei is a natural material that contains anthocyanin substances where in an acidic atmosphere anthocyanin substances provide a red colour so that it can be used as an alternative dye to replace eosin. The aim of this study was to determine the microscopic picture of worm egg preparations using alternative dyes of red dragon fruit peel extract. The research method used is an experimental method. The results showed that the red dragon fruit peel extract dye with a concentration variation of 2%, 5%, and 10% did not colour the worm eggs. So the red dragon fruit peel extract dye is still not optimal for use as an alternative dye for coloring worm eggs.

Susceptibility Test of Aedes aegypti Larvae to Temefos (Abate) Tuti Maula Fauziah; Firda Yanuar Pradani; Ary Nurmalasari
Ad-Dawaa : Journal of Pharmacy Vol. 1 No. 2 (2023): Ad-Dawaa: Journal of Pharmacy (November 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/dwj.v1i2.370

Dengue fever is an infectious disease transmitted by the Aedes aegypti mosquito. Temefos is a tool used in controlling Aedes aegypti larvae and its use for a long time without based on rules can cause resistance. This study aims to determine the susceptibility status of Aedes aegypti larvae to Temefos in the Ciamis and Cimaragas areas. The method of this research is experimental with samples obtained from residential areas in Ciamis and Cimaragas sub-districts then hatched into instar IV larvae at the Parasitology Laboratory of STIKes Muhamamadiyah Ciamis in May 2019. The results of the test on temefos 0.1 g showed that Aedes aegypti larvae from both regions obtained the results of Ciamis (100%) and Cimaragas (100%) sub-districts were still susceptible to temefos. From these results it can be concluded that the test larvae from both areas are still susceptible to temefos so that the tool can still be used as a larvicide, especially in controlling Aedes aegypti mosquitoes.

Parasitic Disease Education in Selacai Village Community Cipaku District Ciamis Regency Ary Nurmalasari; Rahma Anggi Putri Pertiwi; Niva Puspita; Ai Resa Nuraeni; Bilal Ali Faturrahman; M.Fahreza Kencana; Setiofani Utami; Katya Gifanovia; Epi Amalia
Daarul Ilmi: Jurnal Pengabdian Kepada Masyarakat Vol. 1 No. 1 (2023): Daarul Ilmi: Jurnal Pengabdian Kepada Masyarakat (Januari-Juni 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/daipkm.v1i1.230

Introduction: Parasitic diseases are still largely unknown and often overlooked. Objective: The purpose of carrying out community service activities in the form of PPKM (Public Health Development Practices) is to increase public awareness of the importance of health. This activity is also carried out to help by introducing parasitic diseases to the people of Ciheras Hamlet, Selacai Village so that they are more responsive and dangerous of parasitic diseases, and are able to apply a clean and healthy lifestyle. Method: The method is carried out by observation, namely looking at the description of the environment around Ciheras Hamlet, Selacai Village. Result: In the PPKM program carried out in Ciheras Hamlet, Selacai Village, education was carried out using an approach in the form of recitation and provision of material displayed in a power point about parasitic diseases (helminthiasis). Conclusion: All work programs have been successfully carried out with full support by the community.

EKSTRAK DAUN JATI MUDA UNTUK PEWARNA PEMERIKSAAN SEDIMEN URINE Doni Setiawan; Atun Farihatun; Ary Nurmalasari; Euis Tia Istianah; Mujahidah Basarang; Ellies TS Maulidiyanti2
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.20647

Urinalysis examination is needed to find information and determine the condition of the kidneys, urinary tract or other organs such as the liver, pancreas, and bile ducts. A urinary sediment examination aims to see organic and inorganic elements in the urine by preparing using Sternheimer Malbin stain reagents. Sternheimer Malbin stain has the disadvantage that it is a synthetic chemical. Stain from natural ingredients becomes a solution as an alternative to reduce the use of synthetic chemicals. Natural stains with the same characteristics and widely tested for bacterial and fungal stains are natural stains from teak leaf extract. Young leaf extract contains anthocyanins in red colour. This study aimed to determine the ability of ethanol extract from young leaves as a natural stain for urine sediment examination. The research method is an experiment with the Static Group Comparison research design. The population and samples in this study are teak leaves determined at the Galuh University Laboratory of the Biology Study Program with the Latin name (Tectona grandis L.f.). The results of the Fisher Exact test obtained a p (value) <0.050, so there was no significant difference between sediments without stain, Sternheimer Malbin, and ethanol extract of young leaves in colouring urine sediments. The conclusion is that the dye of ethanol extract from young teak leaves effectively colours the components of urine sediment, namely epithelial cells, leukocyte cells, and erythrocyte cells with red colour. Keywords : Anthocyanins, Alternative Dyes, Sternheimer malbin

EKSTRAK DAUN JATI MUDA UNTUK PEWARNA PEMERIKSAAN SEDIMEN URINE Doni Setiawan; Atun Farihatun; Ary Nurmalasari; Euis Tia Istianah; Mujahidah Basarang; Ellies TS Maulidiyanti2
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.20647

Urinalysis examination is needed to find information and determine the condition of the kidneys, urinary tract or other organs such as the liver, pancreas, and bile ducts. A urinary sediment examination aims to see organic and inorganic elements in the urine by preparing using Sternheimer Malbin stain reagents. Sternheimer Malbin stain has the disadvantage that it is a synthetic chemical. Stain from natural ingredients becomes a solution as an alternative to reduce the use of synthetic chemicals. Natural stains with the same characteristics and widely tested for bacterial and fungal stains are natural stains from teak leaf extract. Young leaf extract contains anthocyanins in red colour. This study aimed to determine the ability of ethanol extract from young leaves as a natural stain for urine sediment examination. The research method is an experiment with the Static Group Comparison research design. The population and samples in this study are teak leaves determined at the Galuh University Laboratory of the Biology Study Program with the Latin name (Tectona grandis L.f.). The results of the Fisher Exact test obtained a p (value) <0.050, so there was no significant difference between sediments without stain, Sternheimer Malbin, and ethanol extract of young leaves in colouring urine sediments. The conclusion is that the dye of ethanol extract from young teak leaves effectively colours the components of urine sediment, namely epithelial cells, leukocyte cells, and erythrocyte cells with red colour. Keywords : Anthocyanins, Alternative Dyes, Sternheimer malbin

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