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Almond as Alternative Media for Growth of Staphylococcus aureus and Escherichia coli Ary Nurmalasari; Leni Marlina; Undang Ruhimat; Rahayu Nirmatul Mutmainah
JURNAL KESEHATAN STIKes MUHAMMADIYAH CIAMIS Vol. 9 No. 2 (2022): Jurnal Kesehatan (Oktober 2022)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jurkes.v9i2.344

The high cost of media for microbial culture encourages researchers to create alternative media using local raw materials at low prices. One of the most important media compositions for bacterial growth is protein. This content can be obtained from nuts, one of which is almonds (Prunus dulcis). The purpose of this study is to find natural media that has the potential for the growth of microorganisms at a low price. The research method used is an experimental method to determine the growth of Escherichia coli and Staphylococcus aureus bacteria on natural media of almonds. The results showed the growth of Escherichia coli and Staphylococcus aureus bacteria in this alternative media of almonds. Based on the results of the study, it can be concluded that almond nut media can be applied as an alternative medium in laboratory research, especially in the field of_microbiology. It is suggested that further research should be carried out on natural media which are sources of carbohydrates and proteins as alternative growth media for bacteria.

Description of the Behavior of Aedes aegypti Mosquito Laying Eggs in Water Contaminated with Animal Feces and Soil Firda Yanuar Pradani; Rahayu Nirmatul Mutmainah; Sela Marlina
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 1 (2023): Mukhtabar: Journal of Medical Laboratory Technology (April 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i1.216

Background & Objective This study aims to determine the egg-laying behaviour of Aedes aegypti in water contaminated with animal waste and soil, count eggs caught in cages, observe and count the number of healthy and damaged eggs, and compare the number of eggs from each water medium. Method This study is descriptive, the data were analyzed using the SPSS application, presented in tabular form, and explained in a narrative manner. Result The results of observations of egg-laying behaviour indicate that water contaminated with animal waste and soil can be a breeding ground for the Aedes aegypti. Conclusion Based on the results of observations of the behaviour of Aedes aegypti in water media contaminated with animal faeces and soil, it shows a distinctive behaviour, that water contaminated with animal faeces and soil is favoured by Aedes aegypti as a place to lay eggs and breed. The total number of eggs caught was 1,716, with the number of healthy eggs ( 1,219) and the number of damaged eggs (497). Differences in the content contained in the water media affected the laying of Aedes aegypti eggs but did not affect the number of healthy or damaged eggs.

Screening of Pulmonary Tuberculosis in Household Contacts With Acid Fast Bacteria (AFB) Positive Pulmonary Tuberculosis Patients in Ciwangi Village, Balubur Limbangan Subdistrict, Garut District Shinta Nuriah; Undang Ruhimat; Rivana Aryadi; Rahayu Nirmatul Mutmainah
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 1 (2023): Mukhtabar: Journal of Medical Laboratory Technology (April 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i1.218

Background & Objective Tuberculosis is an infectious disease caused by acid-fast bacteria, namely Mycobacterium tuberculosis, which mainly attacks the lungs. Pulmonary tuberculosis is a global health problem with the second deadliest case after HIV/AIDS infection, and ranks third with the most TB cases after India and China. The main source of transmission is patients with active TB pulmonary tuberculosis who can transmit to people around them. This study aims to determine whether pulmonary TB transmission occurs in people who are in household contact with patients with acid-fast bacteria pulmonary TB active. Method This study is descriptive, using the Ziehl neelsen examination method, with a total sampling technique, conducted on 34 people who are in household contact with pulmonary TB patients in Ciwangi Village, Balubur District, Limbangan. Result Based on the examination results in people who are in contact with people with TB, the results are negative, the presence of the bacteria that cause TB. Conclusion The results of Pulmonary Tuberculosis screening conducted on 34 people from 13 heads of families, it can be concluded that the results of TB microscopy with the Ziehl neelsen technique were negative.

Erythrocyte Index and Mentzer Index in Fathers of Thalassemia Patients Rhama Adiguna; Doni Setiawan; Undang Ruhimat; Rahayu Nirmatul Mutmainah
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 1 (2023): Mukhtabar: Journal of Medical Laboratory Technology (April 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i1.219

Background & Objective Thalassemia is a blood disorder caused by genetic factors that cause the globin chain in hemoglobin to not function normally. It is the most common hereditary disease in the world and in Ciamis Regency in 2021 there were 191 patients. Thalassemia prevention is carried out by screening to identify thalassemia carriers. Hematological examination in the form of erythrocyte index (MCV, MCH, MCHC) and mentzer index has been known to determine thalassemia screening. The purpose of this study was to determine the description of erythrocyte index and mentzer index in thalassemia patient's father. Method The method used in this study is descriptive method. The population in this study were members of POPTI (Parents Association of Thalassemia Patients) Ciamis Regency. The sample in this study were 30 samples. Result Based on the results of the study obtained the results of erythrocyte index (MCV, MCH) low (MCHC) normal and Mentzer index obtained the results of 20 samples (66%) suspected as thalassemia carrier, 5 samples (17%) suspected iron deficiency anemia and according to the reference value there were 5 samples (17%). Conclusion The results of a study conducted on 30 fathers of thalassemia patients showed that 83% of fathers of thalassemia patients had MCV and MCH values below the normal threshold and 73% had normal MCHC values. Furthermore, from the results of the Mentzer index examination, it is known that 66% of the fathers of thalassemia patients are suspected of having minor thalassemia, 17% are suspected of having iron deficiency anemia disorders and 17% have a normal Mentzer index.

Overview of Total Cholesterol Levels at Productive Age in the Imbanagara Health Center Working Area Intan Fandini; Undang Ruhimat; Rivana Ariyadi; Rahayu Nirmatul Mutmainah
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 2 (2023): Mukhtabar: Journal of Medical Laboratory Technology (October 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i2.368

Background & Objective: Total cholesterol is the total amount of HDL, LDL, and VLDL cholesterol in each deciliter of blood. Risk factors that can affect total cholesterol levels are age, gender, sleep patterns, genetics, physical activity, and body mass index. This study aims to determine the description of total cholesterol levels in productive age in the Imbanagara Health Center Working Area. Method: This research method is descriptive research with a purposive sampling technique and involves 31 respondents the instrument used is a photometer. This research was conducted in the Imbanagara Health Center working area from May to June 2023. Result: The results showed that the total cholesterol levels of 31 respondents, were 25 people (81%) with normal total cholesterol levels, 5 people (16%) with high threshold total cholesterol levels, and 1 other person (3%) with high total cholesterol levels. Conclusion: It can be concluded that of the 31 respondents in the Imbanagara Health Center work area, the average has normal Total Cholesterol results.

Almond as Alternative Media for Growth of Staphylococcus aureus and Escherichia coli Nurmalasari, Ary; Marlina, Leni; Ruhimat, Undang; Mutmainah, Rahayu Nirmatul
JURNAL KESEHATAN STIKes MUHAMMADIYAH CIAMIS Vol. 9 No. 2 (2022): Jurnal Kesehatan (Oktober 2022)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jurkes.v9i2.344

The high cost of media for microbial culture encourages researchers to create alternative media using local raw materials at low prices. One of the most important media compositions for bacterial growth is protein. This content can be obtained from nuts, one of which is almonds (Prunus dulcis). The purpose of this study is to find natural media that has the potential for the growth of microorganisms at a low price. The research method used is an experimental method to determine the growth of Escherichia coli and Staphylococcus aureus bacteria on natural media of almonds. The results showed the growth of Escherichia coli and Staphylococcus aureus bacteria in this alternative media of almonds. Based on the results of the study, it can be concluded that almond nut media can be applied as an alternative medium in laboratory research, especially in the field of_microbiology. It is suggested that further research should be carried out on natural media which are sources of carbohydrates and proteins as alternative growth media for bacteria.

Utilization of Purple Sweet Potato Juice As an Alternative Violet Crystal in Coloring Streptococcus sp Bacteria and Escherichia coli Ruhimat, Undang; Mutmainah, Rahayu Nirmatul; Ramdan, Siti Rahmah Kurnia; Firdaus, Nabil Ridla
JURNAL KESEHATAN STIKes MUHAMMADIYAH CIAMIS Vol. 11 No. 2 (2024): Jurnal Kesehatan (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jurkes.v11i2.769

Gram staining is a staining method used to distinguish bacterial species into two major groups, namely Gram-positive and Gram-negative, Crystal violet is the primary (main) dye that will colour the target microorganisms. Staining in bacteria can be replaced using alternative substances with low cost, readily available and harmless, one of which uses substances that have anthocyanin content, namely purple sweet potato. The purpose of this study was to determine the results of using purple sweet potato as an alternative substance to replace crystal violet in staining Streptococcus sp and Escherichia coli bacteria. The method used is experimental. Based on the results of research that has been done on the use of purple sweet potato juice as an alternative substance to crystal violet in gram staining of Streptococcus sp and Escherichia coli bacteria, it shows that purple sweet potato material can be used at pH 6.5 and 5.7 while at pH 5.5 and 5 the bacteria are not perfectly coloured. So it can be concluded that purple sweet potato juice can be used as an alternative substance to Crystal violet in gram staining.

Overview of Routine Blood Test Results in Rheumatoid Arthritis Patients in Ciamis Regency Mutmainah, Rahayu Nirmatul; Rabbani, Muhammad Abdul Hadi; Hanif, Rizal
Mukhtabar Journal of Medical Laboratory Technology Vol 3 No 2 (2025): Mukhtabar: Journal of Medical Laboratory Technology (October 2025)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v3i2.787

Background & Objectives: Based on data from the Ciamis District Health Service Profile (2022), rheumatoid arthritis (RA) ranks 6th out of the 10 most common diseases at community health centers, with 16,195 cases. Systemic inflammation in RA can affect blood cell productivity. This has the potential to cause blood abnormalities, which can lead to diseases such as anemia, leukemia, or blood clotting problems. Routine blood tests are performed as a screening test to determine blood values in RA patients. The purpose of this study was to determine the results of routine blood tests in RA patients in Ciamis Regency. Method: This study used a descriptive method with total sampling technique. The examination was conducted on RA patients in Ciamis Regency in May 2024 with a sample size of 20 respondents. The study used secondary data that was processed into diagrams and then explained narratively. The study used a hematology analyzer located in the hematology laboratory of Ciamis Regional General Hospital. Results: This study found that the percentage of normal blood values for erythrocyte parameters was 70%, hemoglobin was 70%, hematocrit was 70%, average erythrocyte index was normal at 90%, leukocytes were 95%, average leukocyte types were normal at 80%, and thrombocytes were 90%. Low values were found in the erythrocyte (30%), hemoglobin (30%), hematocrit (30%), MCV (25%), MCH (10%), RDW SD (10%), and lymphocyte (11%) parameters. Meanwhile, high values were found in the parameters of white blood cells (10%), lymphocytes (8%), monocytes (15%), neutrophils (10%), eosinophils (40%), basophils (5%), and platelets (10%). Conclusion: It can be concluded that the values for erythrocytes, hemoglobin, hematocrit, erythrocyte indices, leukocytes, leukocyte types, and platelets were generally within the normal range.

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