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All Journal HAYATI Journal of Biosciences MEDIA KONSERVASI Jurnal Veteriner Jurnal Ilmiah Pendidikan Pancasila dan Kewarganegaraan Jurnal Penelitian Pendidikan IPA (JPPIPA) AL-HAYAT: Journal of Biology and Applied Biology JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Jurnal Kedokteran Hewan
LUTHFIRALDA SJAHFIRDI
Departemen Biologi Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Indonesia, Jakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Electrical & Electronics Engineering Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Veterinary

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Journal : Jurnal Veteriner

 1 
Physiological Response of Bligon Buck to Transportation: Relation to Level of Thyroid Hormone Pudji Astuti; Sarmin -; Asmarani Kusumawati; Claude Mona Airin; Hera Maheshwari; Luthfiralda Sjahfirdi
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Transportated animals may subject to a variety of physical stimuli including metabolism, crowding,noise, handling, isolation, agitation, and extreme temperature .The aim of this study was to determine thechanges of serum T4 and T3 concentration, during animals transportation. Six adult Bligon buck with bodyweight ranging from 26-30 Kg were used in this study. Two weeks prior to the experiment, the animalswere given anthelmintic Albendazole to eliminate egg worm. All animals were fed standard diet in theirpen at 10% of their body weight per head daily and commercial concentrate also given everyday. Freshwater was provided ad libitum. All animals were transported around village for 16 hours starting from18.00 pm until 10.00 am in open small truck (3 x 2 m); eye contact each others would be possible. Bloodsamples were withdrawn from jugular vein using vacutainer tubes containing heparin into 1.5 mL glasstubes, then centrifuged at 500 g for 15 minutes. Plasma was collected to be stored at –20° C. The bloodwere collected every 4 hours from 8 hours before transportation ( at 10.00 am, 14.00 pm and 18.00 pm)until the time of arriving after transportation at 10.00 am. Plasma was harvested and stored at –20° Cuntil T3 and T4 concentrations were measured using ELISA method (enzyme linked immunosorbentassay) product DRG, Germany. The result showed that transportation of Bligon bucks for 16 hours havean affect on level of T4 only (P<0.05) and not for T3 concentration (P>0.05) due to physical stimuli such ascrowding, heat stress, noise, handling would be discarded so that the metabolic process was stable. Duringtransportation, decreasing of T4 levels indicated conversion of T4 to T3 to form active hormone.
Fourier Transform Infrared Sebagai Metode Alternatif Penetapan Tingkat Stres pada Sapi (FOURIER TRANSFORM INFRARED AS AN ALTERNATIVE TOOL FOR DETERMINING OF STRESS IN COW) Pudji Astuti; Claude Mona Airin; Slamet Widiyanto; Amelia Hana; Hera Maheshwari; Luthfiralda Sjahfirdi
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Stress in animal is a condition of nonspesific discomfort which cause of non-specific immune defects,failure of reproduction, and decreased of meat carcass until the death of  animals. To determine stress ofcattle,  it will be invented stress detector using a non-invasive method based on the spectroscopy FourierTransform Infrared (FTIR). Basically,  FTIR will detect component in compound of cathecolamine andcortisol as ketone (= O) and methyl (= CH 3). Furthermore, each group of components will be detected indifferent of absorbant and wavelength.   The results showed that average level of cortisol in female beefcattle durimg resting eriod was 38,48±21,53 ng/dL, on time of slaughtering were 116,88±112,59 ng/dL. Forbull, which were resting  20,42±9,25 ng/dL; when animal was slaughtered level of cortisol was  67,61±41,62ng/dL. Using FTIR, it was showed that compound of metil was absorbed well. Animals with udder stresscondition have been recorded on the wave lenght of 2777-3456 nm.   It has been concluded level of cortisolon cattle which were resting is significantly different from animal which were slaughtering P(< 0.05),where cortisol would increase drastically. Using Calibration of FTIR indicated resting animals only havefewer value of absorbance than animals which slaughtered.  FTIR is a very prospect method for makingstress indicator.
Pemanfaatan Electronic Nose sebagai Sensor Kimiawi Urin Guna Melacak Birahi Sapi (ELECTRONIC NOSE AS URINARY CHEMICAL SENSOR FOR DETERMINING ESTROUS PHASE IN CATTLE) Pudji Astuti; Claude Mona Airin; Slamet Widiyanto; Luthfiralda Sjahfirdi; Hera Maheshwari
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The timing of artificial insemination relies largely on behavioral observation of estrus. The problemfaced is that not all cattle shows signs of estrus significantly, which affect the accuracy of insemination,and therefore,the success rate of Artificial Insemination is less than 50%. Recently, the determination ofestradiol levels as an indicator of estrus is done by observation of physical signs and doing ELISA test,which is expensive and provide longer time. In order to solve these problems, a tool estrus detector is madenamely Electronic Nose (EN). Determination of estrus with EN is cheaper because it does not need to usecomplexmaterials, just only use the samples. Mechanism of action of EN is using a sensor that is vaporized,while animals estrus will emit pheromones that are vaporized. Theaim of this study was to determinewhether the stage of estrus can be detected by using EN. Urine of female Ongole Crossbred which maintainedin Kuwang, district of Cangkringan, Yogyakarta, with BCS of 3 was used in this research. The sample wascollected shortly before injection of dinoprost as estrus synchronization then it repeated when cattle got estrus phase. The urinary sample of the estrus cattle was sensitive to methane, propane, butane, whereasin non-estrus cattle, besides the three of these component (methane, propane, butane), sensor was alsocaptured hydrogen sulfide. Furthermore, our electronic nose had been able to distinguish estrus phase andnon-estrus based on domain area.Thus, the Electronic Nose is very prospect used as a detector of estrus incattle. Hydrogen disulfide could possibly be used as an indicator comparison between cow estrus and nonestrus.
Co-Authors Ainy, Noer Sarifah Amelia Hana Ananda, Syafira Dwi Andayani, Noviar Anisa Putri Sukmaningrum Asmarani Kusumawati Bambang Purwantara Claude Mona Airin Dondin Sajuthi ERIC HAYES Firmanza, Nasya Aqila Hadi S Alikodra Hadi, Nestiyanto Hana Nabilah Hapsari Indriastuti HERA MAHESHWARI Ilmia Rosame Islahuddin, Islahuddin Mozes R. Tolihere Nauhal Nazri Halim Nikki Aldi Nikki Aldi, Nikki Oriza Savitri Ariantie Pudji Astuti Pudji Astuti Pudji Astuti PUDJI ASTUTI Pudji Astuti Pudjiastuti, Sri Rahayu Putri Krida Gita P. Putri Krida Gita P. Reviany Widjajakusuma Reviany Widjajakusuma Saka, Raisa Tatum Saka, Raisa Tatum Sarmin - Siti Nursheena Mohd Zain Siti Nursheena Mohd Zain Slamet Widiyanto Tatang Mitra Setia Tatang Mitra Setia Tuty Laswardi Yusuf Wendi Prameswari