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Pilot evaluation of in vitro culture methods for ScSMV reduction in sugarcane PS 864 confirmed by RT-PCR ARIE HAPSANI HASAN BASRI; SITI RASMINAH CHAILANI; BAMBANG TRI RAHARDJO; WIWIT BUDI WIDYASARI
Asian Journal of Agriculture Vol. 10 No. 2 (2026)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g100217

Abstract

Abstract. Basri AHH, Chailani SR, Rahardjo BT, Widyasari WB. 2026. Pilot evaluation of in vitro culture methods for ScSMV reduction in sugarcane PS 864 confirmed by RT-PCR. Asian J Agric 10 (2): g100217. https://doi.org/10.13057/asianjagric/g100217. Sugarcane Streak Mosaic Virus (ScSMV) is transmitted mainly through infected vegetative planting material, yet the feasibility of in vitro culture for producing RT-PCR-negative PS 864 planting material has not been well established. This study aimed to compare three in vitro regeneration pathways (callus, shoot, and meristem culture) in PS 864, with RT-PCR indexing of ScSMV in the regenerated plantlets. Visually symptomless and infected PS 864 plants were grown for 4-5 months as explant sources. Callus culture achieved the highest regeneration rate (~95%), whereas shoot culture was severely limited by contamination (90%), and meristem culture produced no plantlets during the study period. Thirteen samples (six conventional controls and seven in vitro plantlets) were indexed by RT-PCR using SM1/SM2 primer pair. Of the six callus-derived plantlets, four were ScSMV-positive, and two were negative; the single shoot-derived plantlet was also negative. Because no internal amplification control was included, the negative results are provisional and cannot confirm the true absence of the virus. The dataset was small and unequal across pathways (callus, n = 6; shoot, n = 1; meristem, n = 0), precluding robust comparative inference. These findings indicate that callus culture is the most reproducible route for regeneration of PS 864 and that molecular indexing is essential, as ScSMV was detected even in visually symptomless plants. Confirmatory studies with larger, balanced samples and an internal control are needed before these pathways can support virus-free seed-cane programs.
IDENTIFIKASI DAN INVENTARISASI KERAGAMAN SERANGGA PADA BERBAGAI DESAIN PERANGKAP DI TANAMAN BELUM MENGHASILKAN KELAPA SAWIT: IDENTIFICATION AND INVENTORY OF INSECT DIVERSITY IN VARIOUS TRAP DESIGNS IN IMMATURE OIL PALM PLANTS Prayoga, Alfin; Basri, Arie Hapsani Hasan; Ramadhani, Elrisa
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 14 No. 3 (2026)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jurnalhpt.2026.014.3.4

Abstract

Insect diversity in oil palm plantations is influenced by various factors, including the trap design used during inventory activities. This study aimed to identify and inventory insect diversity using various trap designs in immature oil palm plantations and determine the most effective trap design. The study was conducted from February to March 2026 in Central Tapanuli Regency, North Sumatra, using a non-factorial Randomized Complete Block Design (RCBD) with four treatments: Vane Trap, Bucket Trap, Multi-Funnel Trap, and Frame Net Trap, each replicated six times. Observations were conducted for 21 days using a natural attractant consisting of pineapple extract and fermented banana stem solution. The observed variables included the number of insect individuals, taxonomic identification to the species level, ecological roles, Shannon–Wiener diversity index, and evenness index. The results showed that the Multi-Funnel Trap was the most effective trap, producing the highest number of captured insects (248 individuals). A total of 562 individual insects were identified, comprising 4 orders, 10 families, 18 genera, and 19 species, with the order Coleoptera dominating at 86.12%. Based on their ecological roles, insects were predominantly decomposers (52.31%), followed by predators (25.09%) and pests (22.60%). The diversity index values ​​obtained from the Vane Trap, Bucket Trap, and Multi-Funnel Trap were categorized as moderate, whereas the Frame Net Trap showed low diversity. Therefore, the Multi-Funnel Trap was the most effective trap design for insect diversity inventory in oil palm plantations.
Tingkat Efektivitas Rancangan Penyuluhan dalam Pengendalian Hama Penggerek Buah Kopi menggunakan Atraktan Trap pada Kopi Robusta di Kecamatan Marancar Silvi Hafni Sunardi; Linda Tri Wira Astuti; Arie Hapsani Hasan Basri
Journal of Sustainable Agriculture Extension Vol 4 No 2 (2026): Journal of Sustainable Agriculture Extension
Publisher : Politeknik Pembangunan Pertanian Manokwari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47687/josae.v4i2.2129

Abstract

Latar belakang : Hama Penggerek Buah Kopi (PBKo) (Hypothenemus hampei) merupakan hama utama yang menurunkan kualitas dan kuantitas produksi kopi robusta di Kecamatan Marancar, Kabupaten Tapanuli Selatan. Tingkat serangan PBKo mencapai 25%, sedangkan sebagian besar petani masih mengandalkan pestisida kimia dan belum menerapkan teknologi Atraktan Trap sebagai alternatif pengendalian yang ramah lingkungan. Metode: Penelitian ini bertujuan menyusun rancangan penyuluhan pengendalian Hama Penggerek Buah Kopi menggunakan Atraktan Trap, mengkaji tingkat penerimaan sasaran terhadap rancangan penyuluhan. Penelitian dilaksanakan pada bulan Maret hingga Mei 2026 di Kecamatan Marancar, Kabupaten Tapanuli Selatan menggunakan metode deskriptif dengan pendekatan kuantitatif. Tingkat penerimaan sasaran sebagai indikator terhadap efektivitas rancangan penyuluhan berdasarkan aspek materi, metode, media, volume, waktu, lokasi, dan biaya penyuluhan menggunakan skala Likert. Responden penelitian berjumlah 50 petani kopi robusta. Hasil: Hasil penelitian menunjukkan bahwa rancangan penyuluhan memperoleh tingkat penerimaan sebesar 88,44% dengan kategori sangat efektif. Evaluasi dilakukan terhadap tujuh aspek penyuluhan, meliputi materi, metode, media, volume, waktu, lokasi, dan biaya. Hasil penilaian menunjukkan bahwa aspek lokasi memperoleh persentase tertinggi sebesar 90,72%, disusul oleh biaya sebesar 90,50%, volume 89,90%, metode 89,80%, materi 89,68%, waktu 88,80%, serta media sebesar 82,76%. Secara keseluruhan, seluruh aspek berada dalam kategori efektif hingga sangat efektif. Hal ini menunjukkan bahwa rancangan penyuluhan yang disusun telah sesuai dengan kebutuhan, karakteristik, dan kondisi petani kopi robusta di Kecamatan Marancar. Kesimpulan: Rancangan penyuluhan pengendalian Hama Penggerek Buah Kopi menggunakan Atraktan Trap dinilai sangat efektif. Penyuluhan ini berpotensi mendorong penerapan teknologi pengendalian hama yang ramah lingkungan secara berkelanjutan sehingga dapat mendukung peningkatan produktivitas dan kualitas kopi robusta di Kecamatan Marancar, Kabupaten Tapanuli Selatan.
EFFECTIVENESS OF USING ARBUSCULAR MYCORRHIZAL FUNGI AND COMPOST TO INCREASE GOGO RICE PRODUCTION IN SUBOPTIMAL LANDS Arie Hapsani Hasan Basri; Aisar Novita; Taufiq Hidayatullah; Windy Manullang; Silvia Nora; Tience Elizabeth Pakpahan
Jurnal Agrotek Tropika Vol. 14 No. 3 (2026): JURNAL AGROTEK TROPIKA VOL 14, AGUSTUS 2026
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v14i3.11276

Abstract

The expansion of upland rice cultivation into suboptimal lands, particularly Ultisols, represents a strategic approach to increasing national rice production. However, low soil fertility, acidic pH, and limited nutrient availability constrain productivity in such environments. This study aimed to evaluate the effectiveness of Arbuscular Mycorrhizal Fungi (AMF) and compost application in improving the growth and yield of upland rice (Inpago 12) cultivated on Ultisol soils in Deli Serdang and Langkat, North Sumatra. A factorial randomized block design with three factors, AMF dosage (0–600 spores), compost rate (0–5 t ha⁻¹), and location that was implemented with three replications (150 experimental units). Soil analysis confirmed acidic conditions with low nutrient status typical of Ultisols. Five AMF taxa were identified with root colonization ranging from moderate to very high (46.67–100%). ANOVA results revealed that location significantly affected most growth and yield parameters, whereas AMF and compost treatments showed limited statistical effects. The most effective treatment combination was 450 spores with 2 t ha⁻¹ compost in Deli Serdang, which improved tiller number, biomass, and panicle production. These findings indicate that the effectiveness of AMF and compost application in Ultisols is highly site-specific and influenced by local soil characteristics.