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All Journal Jurnal Penelitian Saintek ASPIRATOR Program Kreativitas Mahasiswa - Gagasan Tertulis Jurnal Berkala Epidemiologi Indonesian Journal of Biotechnology JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI Riau Journal of Empowerment Journal of Biotechnology and Natural Science ASPIRATOR - Journal of Vector-Borne Diseases Studies

Soenarwan Hery Poerwanto

Departemen Biologi Tropika Fakultas Biologi, Universitas Gadjah Mada Bulaksumur, Daerah Istimewa Yogyakarta, Indonesia 55281

Author-ID : 384034

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Cloning and Sequencing cDNA Encoding for Rhoptry-2 Toxoplasma Gondii Tachyzoite Local Isolate Artama, Wayan T.; Sari, Yulia; Subekti, Didik Tulus; Poerwanto, Soenarwan Hery; Subandono, Jarot
Indonesian Journal of Biotechnology Vol 10, No 2 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.765 KB)

Rhoptry protein belongs to an excretory and secretory antigens (ESAs) that play an important role during activepenetration of parasite into the cell target. This protein an able Toxoplasma gondii to actively penetrate targetedcell, meanwhile ESAs protein stimulates intracellular vacuole modification. It is, therefore, after the parasitesuccessfully enter the cell target then Granule (GRA) proteins are responsible for the formation of parasitophorusvacuole, which is protect the fusion with other intracellular compartments such as lysosomal vacuole. Consequently,this parasite is being able to survive and multiply at the cell target. The current study was aimed to clone andsequens cDNA encoding for ROP-2 of local isolated T. gondii tachizoite through DNA recombinant technique.Total ribonucleic acid (RNA) was isolated from tachyzoites of local isolated T. gondii that were grown up in Balb/c mice. Messenger RNA was isolated from total RNA using PolyAtract mRNA Isolation System. Messenger RNA wasused as a template for synthesis cDNA using Riboclone cDNA Synthesis System AMV-RT. EcoRI adaptor fromRiboclone EcoRI Adaptor Ligation System was added to Complementary DNA and than ligated to pUC19. Recombinantplasmid was transformed into E. coli (XL1-Blue). The transformed E. coli XL-1 Blue were plated on LB agarcontaining X-Gal, IPTG and ampicillin. Recombinant clones (white colony) were picked up and grown up in theLB medium at 37oC overnight. Expression of recombinant protein was analysed by immunoblotting in order toidentify cDNA recombinant wich is express ESA of T. gondii local isolate. Recombinant plasmid were isolatedusing alkalilysis method and were elektroforated in 1% agarose gel. The isolated DNA recombinant plasmid wascut using Eco RI and then sequenced through Big Dye Terminator Mix AB1 377A Sequencer using M13 Forward andM13 Reverse primers. The conclusion of this results showed that the recombinant clone was coding for excretoryand secretory protein which has molecular weight of 54 kDa. The DNA alignments of sequence from the clonedgene showed 97% homology with gene encoding for ROP-2 of T. gondii RH isolate.Keywords: Toxoplasma gondii, tachizoite, ESA, complementary DNA, ROP2

NYAMUK SEBAGAI VACCINE: SEBUAH PEMIKIRAN FUTURISTIK Kurniawati, Milla Fajar; Agustia, Fernando Dwi; Khairiskam, Muhammad; Poerwanto, Soenarwan Hery
Program Kreativitas Mahasiswa - Gagasan Tertulis PKM-GT-2014
Publisher : Ditlitabmas, Ditjen DIKTI, Kemdikbud RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.791 KB)

The development of recombinant DNA biotechnology especially lately has opened new expectations. This technology allows to produce a vaccine that has yet to be made. In addition, this technology can also be used to improve existing vaccines, so we get more vaccine is safe and effective. This technological innovation allows the manufacture of vaccines and subunit vaccines idiotipe (therapeutic vaccines). Therefore, we initiated the concept of mosquitoes as Vaccine Delivery Host: A Futuristic Thought as the protection of effective prevention and control as a solution to the crisis facing outbreaks of infectious and parasitic diseases that occur in Indonesia. This concept utilizes a mosquito vector for the production of antigens by recombinant DNA techniques. Where specific antigens of microbial parasites were cloned in bacterial plasmids engineered to increase expression of the inserted gene in the cells of the mosquito vector, so that when the mosquito bites do not transmit disease, but actually increase the immune response to a disease. The aim of this concept is in addition to the protection and control of outbreaks of infectious diseases and parasitic diseases in endemic areas, it is also said in response to the health crisis in contemporary conditions.Keywords: mosquito vector, recombinant DNA, vaccine

Cloning and Sequencing cDNA Encoding for Rhoptry-2 Toxoplasma Gondii Tachyzoite Local Isolate Wayan T. Artama; Yulia Sari; Didik Tulus Subekti; Soenarwan Hery Poerwanto; Jarot Subandono
Indonesian Journal of Biotechnology Vol 10, No 2 (2005)
Publisher : Universitas Gadjah Mada

Rhoptry protein belongs to an excretory and secretory antigens (ESAs) that play an important role during active penetration of parasite into the cell target. This protein an able Toxoplasma gondii to actively penetrate targeted cell, meanwhile ESAs protein stimulates intracellular vacuole modification. It is, therefore, after the parasite successfully enter the cell target then Granule (GRA) proteins are responsible for the formation of parasitophorus vacuole, which is protect the fusion with other intracellular compartments such as lysosomal vacuole. Consequently, this parasite is being able to survive and multiply at the cell target. The current study was aimed to clone and sequens cDNA encoding for ROP-2 of local isolated T. gondii tachizoite through DNA recombinant technique. Total ribonucleic acid (RNA) was isolated from tachyzoites of local isolated T. gondii that were grown up in Balb/ c mice. Messenger RNA was isolated from total RNA using PolyAtract mRNA Isolation System. Messenger RNA was used as a template for synthesis cDNA using Riboclone cDNA Synthesis System AMV-RT. EcoRI adaptor from Riboclone EcoRI Adaptor Ligation System was added to Complementary DNA and than ligated to pUC19. Recombinant plasmid was transformed into E. coli (XL1-Blue). The transformed E. coli XL-1 Blue were plated on LB agar containing X-Gal, IPTG and ampicillin. Recombinant clones (white colony) were picked up and grown up in the LB medium at 37oC overnight. Expression of recombinant protein was analysed by immunoblotting in order to identify cDNA recombinant wich is express ESA of T. gondii local isolate. Recombinant plasmid were isolated using alkalilysis method and were elektroforated in 1% agarose gel. The isolated DNA recombinant plasmid was cut using Eco RI and then sequenced through Big Dye Terminator Mix AB1 377A Sequencer using M13 Forward and M13 Reverse primers. The conclusion of this results showed that the recombinant clone was coding for excretory and secretory protein which has molecular weight of 54 kDa. The DNA alignments of sequence from the cloned gene showed 97% homology with gene encoding for ROP-2 of T. gondii RH isolate.

KEANEKARAGAMAN ACARINA DI PUSAT INOVASI AGRO TEKNOLOGI MANGUNAN Soenarwan Hery Poerwanto; Anggun Handiani; Dila Hening Windyaraini
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Penelitian ini dilakukan untuk mengetahui keanekaragaman Acarina dan faktor lingkungan yang mempengaruhi keberadaan Acarina di Pusat Inovasi Agro Teknologi (PIAT) Mangunan, Yogyakarta. Penelitian ini dilakukan di PIAT Mangunan untuk pengambilan sampel dan Laboratorium Sistematika Hewan bagian Parasitologi Fakultas Biologi UGM untuk preparasi dan identifikasi. Metode yang digunakan adalah ekstraksi sampel menggunakan corong Barlese Tullgren dengan beberapa modifikasi. Sampel diambil pada 3 area vegetasi (sirsak, srikaya, dan sawo) dan setiap area diambil lima titik sampling. Pengukuran lingkungan meliputi kelembaban udara dan suhu udara diukur dengan alat higrometer, pH, dan suhu tanah dengan soil tester digital, serta kelembaban tanah dengan soil tester Takemura DM-5. Acarina yang diperoleh dilihat di bawah mikroskop dan di-mounting dengan larutan hoyer’s. Identifikasi Acarina menggunakan buku identifikasi A Manual of Acarology. Data yang diperoleh dari penelitian dianalisis menggunakan indeks keanekaragaman Shannon-Wiener. Berdasarkan identifikasi Acarina yang telah dilakukan, didapatkan 20 Family dan 28 Genus dan 399 cacah individu. Tingkat keanekaragaman Acarina termasuk dalam kategori sedang. Faktor lingkungan yang mempengaruhi kehadiran Acarina adalah suhu dan kelembaban.THE DIVERSITY OF ACARINA IN AGRO TECHNOLOGY INNOVATION CENTER MANGUNANThis study was aimed at determining the diversity of Acarina and environmental factors that affect the existence Acarina at Agro Technology Innovation Center (ATIC) Mangunan, Yogyakarta. This study was conducting at ATIC Mangunan for sampling and the Animal Systematics Laboratory of the Parasitology, Faculty of Biology, Gajah Mada University for preparation and identification. The method used was sample extraction using Barlese Tullgren funnel with several modifications. Samples were taken at 3 vegetation areas (soursop, sarikaya a.k.a Annona squamosa, and sapodilla) and each area was taken five sampling points. The environmental measurements include air humidity and air temperature measured by means of a hygrometer, pH and soil temperature with digital soil tester, and soil moisture with a soil tester Takemura DM-5. Acarina obtained was seen under a microscope and mounted with Hoyer’s solution. The identification of Acarina using identification book A Manual of Acarology. The data obtained from the study were analyzed using the Shannon-Wiener diversity index. Based on Acarina’s identification, 20 families and 28 genera and 399 individual numbers were obtained. The level of diversity of Acarina is categorized as the medium category. The environmental factors that influence the presence of Acarina are temperature and humidity.

Identification of Culicidae Family Diversity as Vector Control Management and Mosquito-Borne Disease Prevention in Universitas Gadjah Mada, Yogyakarta Dila Hening Windyaraini; Fiola Tiarani Siregar; Asti Vanani; Titi Marsifah; Soenarwan Hery Poerwanto
JURNAL KESEHATAN LINGKUNGAN Vol. 12 No. 1 (2020): JURNAL KESEHATAN LINGKUNGAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v12i1.2020.1-9

Introduction: Family Culicidae was the presence of a mosquito that had a potential vector to cause the spread of dengue fever and some diseases. Mosquito diversity could be different due to human and environmental factors in those regions. This study aims to identify mosquito (family Culicidae) diversity and characteristics of breeding places as vector control management and mosquito-borne disease prevention in the area of Universitas Gadjah Mada, Yogyakarta. Method: The study was conducted from April to September 2018. A sampling of mosquito larvae and observation of mosquitoes breeding places characteristics were carried out inside and outside the Universitas Gadjah Mada campus building which was divided into 5 clusters, there were Science and Engineering cluster, Medica, Agro, Vocational School, and Social Science. Mosquito diversity in the Universitas Gadjah Mada campus area was analyzed used the Shannon-Wienner diversity index. Result and Discussion: Mosquitoes found in the area of Universitas Gadjah Mada consist of two phases with total number 153 larvae and pupae, namely Aedes aegypti, Aedes albopictus, Anopheles spp., and Culex spp. The mosquito with the highest number of larvae and pupae was Aedes albopictus, while Anopheles spp. Only found 1 pupa phase. The number of obtained containers was 50 containers, with 6 positive containers of mosquito larvae. Mostly, the type of container found with mosquito larvae was bucket. For each container observed, the temperature and pH of water in the container were measured and it was found that the water temperature reached 24 – 28°C with pH 6 – 7. Conclusion: Mosquito diversity from all of the areas in Universitas Gadjah Mada was grouped as a medium category, with the greatest number of mosquito larvae were found in Science and Engineering cluster. Containers were located in the open area had more mosquito larvae. Mosquito control is focused on environmental management, biological control, and chemical use.

Habitats Characteristic and the Resistance Status of Aedes sp. Larvae in the Endemic Areas of Dengue Haemorrhagic Fever in Sewon Subdistrict, Bantul Regency, Special Region of Yogyakarta Soenarwan Hery Poerwanto; Defriana Lutfi Chusnaifah; Giyantolin Giyantolin; Dila Hening Windyaraini
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 2 (2020): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.55494

Dengue Hemorrhagic Fever (DHF) is caused by Dengue Virus and transmitted by female Aedes mosquito which spread almost all over the world. Aedes sp. mosquito lives cosmopolitan and breeds in wet environments. Panggungharjo and Bangunharjo villages were categorized as endemic and non-endemic DHF areas, respectively. The aims of this research were to study the characteristics of Aedes sp. mosquito breeding sites, the identity of presence species found in the sites, and the resistance status of Aedes sp. mosquitoes against organophosphate insecticide. The method was using a larval survey which consists of 200 houses as respondents located in Panggungharjo and Bangunharjo villages. The larval resistance was tested by a biochemical method since resistance could be associated with esterase enzyme activity. The characteristics of mosquito breeding sites that found were open containers, filled with clear and calm water, dark and rough wall surfaces, the bottom surface was not directly in contact with the ground, the water temperature was 27-29 °C, pH 6.5-7, and not directly exposed to sunlight. There was only one species of mosquito was found, Aedes aegypti. The resistance test of Aedes sp. larvae showed that Aedes sp. larvae population from Panggungharjo village were susceptible, and Aedes sp. larvae from Bangunharjo village were in moderate resistant against organophosphate insecticide.

LARVA TREMATODA PADA SIPUT AIR TAWAR DI AREAL PERSAWAHAN DAERAH ISTIMEWA YOGYAKARTA Soenarwan Hery Poerwanto; Dian Antika Kusuma Dewi; Giyantolin Giyantolin
BERITA BIOLOGI Vol 19, No 3B (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3B.3939

The life cycle of Digenea subclass trematodes that mostly involves snails as intermediate host in the larval stage in the form of sporocysts, redia and cercariae. This study aims to determine the species of freshwater snails, populations and stages of trematoda larvae that infect freshwater snails in the paddy fields and also the factors that influence the population of trematoda larvae. This research method uses purposive random sampling. Snails were collected from paddy fields in Sleman Regency, Yogyakarta City and Bantul Regency, Special Region of Yogyakarta. Snails and Trematodes larvae were identified based on morphological characters with a microscope. The results showed that the species of freshwater snail as an inang antara of Trematode was Lymnaea sp. (96.9%), Pomacea canaliculata (2.6%), and Brotia sp. (0.5%). The total population of trematoda larvae in freshwater snails was 4329 individuals with the largest population in Sleman Regency (2784 individuals). Trematode larval stages found were sporocysts (4.8%), redia (8.3%), Leptocercous type cercariae (35.2%), and Furcocercous type cercariae (51.7%). The most trematode larvae found in fresh water snail was Furcocercous type cercariae. Factor affecting trematode larvae population are the species and abundance of freshwater snail.

POTENTIAL BREEDING SITES AND ORGANOPHOSPHATE RESISTANCE STATUS OF AEDES SPP. IN YOGYAKARTA Nila Qudsiyati; Fitri Ainun Nazara; Jeremy Tertius Kilima; Soenarwan Hery Poerwanto
Jurnal Berkala Epidemiologi Vol. 10 No. 1 (2022): Jurnal Berkala Epidemiologi (Periodic Epidemiology Journal)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbe.V10I12022.30-39

Background: The discovery of Aedes spp. in the breeding sites is the leading cause of the high incidence of Dengue Hemorrhagic Fever (DHF) in Yogyakarta in 2019. This incidence can be calculated based on the Container Index (CI), House Index (HI), and Breteau Index (BI). Efforts to control the density of Aedes spp. The use of organophosphate insecticides in the long term and with the wrong dose will cause resistance. Purpose: This research was conducted to determine the potential breeding sites, the density, and the organophosphate resistance status of Aedes spp. in Yogyakarta. Methods: A literature review with the criteria of referenced journals from 2011-2020, national journals accredited at least Sinta 5 to Sinta 1 and international journals at least Q4 to Q1. Results: This study shows that the potential breeding sites for Aedes spp. in Yogyakarta, in general, are both indoors or outdoors, stagnant clean water, opened, not exposed to direct sunlight, and the duration of not eradicating the mosquito nests > 1 month. Aedes spp. in Yogyakarta has a high population density, so it is at high risk of transmitting dengue cases. The resistance status of Aedes spp. in various regions of Yogyakarta are categorized as resistant to organophosphate insecticides. Conclusion: Literature review requires field research also, implementation of prevention through the mosquito nests eradication program and 3M (Shutting, Draining, and Burying), and the replacement of insecticides types other than organophosphates.

Pemberdayaan masyarakat hidup sehat bebas vektor nyamuk melalui konsep ecohealth village berbasis education for sustainable development Giyantolin Giyantolin; Soenarwan Hery Poerwanto; Azinuddin Ikram Hakim; Muflihah Abustani; Robi Wibowo
Riau Journal of Empowerment Vol 2 No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

On 2018, the incidence of Dengue Hemorrhagic Fever (DHF) occurred in Sukoharjo District, which reached 42 villages with the highest in Sukoharjo District, one of which was in Kelurahan Kenep. More understanding is needed about the education of the dangers of mosquitoes, the characteristics of breeding and the formation of movements, namely through active, creative, innovative and solutive community empowerment. The establishment of the program carried out was the empowerment of mosquito-free healthy communities through the Ecohealth Village concept based on Education for Sustainable Development, which is tied to social, environmental and economic. This program is also carried out with the prevention of various perspectives, such as the use of used materials as mosquito traps, the spread of anti-mosquito plants, and movement of posters to encourage people to live mosquito-free healthy lives and the establishment of one Jumantik Monitoring Household. The results of this program influence the understanding of creative, innovative education and solutions to mosquitoes. During and post-implementation, there have been no cases of dengue. The program provides a significant influence in realising a mosquito-free healthy village.

Karakteristik Habitat Larva Nyamuk dan Kepadatan Nyamuk Dewasa (Diptera: Culicidae) di Kabupaten Jembrana, Provinsi Bali (Analisis Data Sekunder Rikhus Vektora 2017) Tri Wahono; dionisius widjayanto; Soenarwan Hery Poerwanto
ASPIRATOR - Journal of Vector-borne Disease Studies Vol 14 No 1 (2022): Jurnal Aspirator Volume 14 Nomor 1 2022
Publisher : Loka Litbang Kesehatan Pangandaran

Abstrak. Nyamuk berperan langsung dalam penyebaran berbagai penyakit tular vektor di negara tropis. Tiga genus nyamuk utama penyebaran penyakit di Indonesia adalah Aedes, Culex, dan Anopheles. Kabupaten Jembrana, Provinsi Bali merupakan daerah endemis untuk DBD, malaria maupun filariasis. Kabupaten Jembrana masih melaporkan kasus DBD dan malaria serta dalam tahap pemberian obat pencegahan massal filariasis pada tahun 2020. Pengendalian vektor nyamuk dipengaruhi berbagai hal seperti karakteristik habitat larvanya dan kepadatan nyamuk dewasa. Penelitian menggunakan data Rikhus Vektora 2017 di Kabupaten Jembrana, Provinsi Bali dengan melihat karakteristik habitat larva, pengukuran parameter air, dan kepadatan nyamuk dengan perhitungan Man Hour Density, serta pengukuran parameter lingkungan pada semua tipe ekosistem. Habitat larva Aedes sp. berupa genangan air tawar yang tidak bersentuhan dengan tanah; Culex sp. berupagenangan air tawar bersentuhan dengan tanah; dan Anopheles sp. genangan air tawar-payau yang bersentuhan dengan tanah dengan parameter air yang berbeda, namun masih dalam rentang yang tidak jauh. Kepadatan nyamuk didominasi oleh Culex sp. di semua ekosistem kecuali pada ekosistem pantai dekat dengan pemukiman didominasi oleh Aedes sp. Semua nyamuk dewasa (Aedes sp., Culex sp., dan Anopheles sp.) di Kabupaten Jembrana lebih bersifat bersifat zoofilik. Perilaku semua nyamuk dewasa berbeda di setiap ekosistem, dapat bersifat endofagik maupun eksofagik. Parameter lingkungan di Kabupaten Jembrana memilikipotensi untuk mendukung perkembangbiakan nyamuk.

Co-Authors Ananmaimuna, Jovika Syifa Anggun Handiani Asti Vanani Azinuddin Ikram Hakim Defriana Lutfi Chusnaifah Desak Putu Raka Paramitha Dhea Ginawati Dian Antika Kusuma Dewi Didik Tulus Subekti Didik Tulus Subekti Dila Hening Windyaraini dionisius widjayanto Dwi Umi Siswanti Fernando Dwi Agustia, Fernando Dwi Fiola Tiarani Siregar Fitri Ainun Nazara Giyantolin, Giyantolin Hari Purwanto Jarot Subandono Jarot Subandono Jeremy Tertius Kilima Luqman Rasyid Ridhwan Malika, Pradhika Cikal Milla Fajar Kurniawati, Milla Fajar Muflihah Abustani Muhammad Khairiskam, Muhammad Nila Qudsiyati Nilamsari, Regina Robi Wibowo Tamaulina Br Sembiring Titi Marsifah Tri Wahono Tri Wahono Untari, Ludmilla Fitri Vidhantyka, K.H. Mahadhevy Ryrathna Wayan T. Artama Wayan T. Artama Widjayanto, Dionisius Yulia Sari

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