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All Journal BIOMA : Jurnal Biologi Makassar Stannum : Jurnal Sains dan Terapan Kimia Jurnal Ilmiah Kesehatan Rustida Jurnal Pengmas Kestra (JPK)
Visensius Krisdianilo
Departement Of Medical Laboratory Technology, Faculty Of Pharmacy, Institut Kesehatan Medistra Lubuk Pakam, Sudirman Street No. 38, Deli Serdang, North Sumatera, 20512. Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Education Health Professions Medicine & Pharmacology Nursing Public Health

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Analysis of Total Terpenoids from Maniltoa Grandiflora (A. Gray) Scheff Leaves Using TLC and HPLC Methods Jhon Patar Sinurat; Visensius Krisdianilo; Reh Malem br Karo; Rinaldo Berutu
Stannum : Jurnal Sains dan Terapan Kimia Vol 2 No 2 (2020): Oktober 2020
Publisher : Department of Chemistry - Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jstk.v2i2.1976

Abstract

Terpenoids screening were carried out using Liebermann Burchard and Salkowski reagent on the extract of Saputangan leaves. It showed that the leaves contained terpenoid compounds with appeared of a reddish brown ring in the extract and a reddish brown stain appeared on the TLC plate tested with 1% CeSO4 reagent in 10% H2SO4. The macerate of saputangan leaves processed separation using the partition method (Liquid-liquid Extraction). Extracts dissolved with methanol were partitioned with n-Hexane and then partitioned between aquadest and ethyl acetate in a ratio of 1: 1 to obtain 50 g of total terpenoids. Furthermore, TLC analysis was performed on total terpenoids using n-hexane: ethyl acetate (80:20 v/v) solvent to obtain 11 separate stains on the TLC plate with different Rf each. Analysis was enhanced in HPLC using 100% acetonitrile and 0.1% phosphoric acid at a wavelength of 210 nm, a flow rate of 0.500 mL/min and eluted for 30 minutes. Based on the HPLC results, there were 25 peaks which indicated the presence of total terpenoid compounds with the highest peak being peak no. 8 (ret.time's 6.234, area's 8503532 and height's 276032), peak no. 9 (ret.time's 6.674, area's 3322572 and height's 141859) and peak no. 10 (ret.time's 7.288, area's 2758231 and height's 103927)
Identifikasi Bakteri Coliform pada Susu Kedelai Menggunakan Metode MPN (Most Probable Number) Vincentia Ade Rizky; Saadah Siregar; Asvia Rahayu; Visensius Krisdianilo; Suci Mustika Mawardani; Dian Pratiwi
Jurnal Ilmiah Kesehatan Rustida Vol 10 No 1 (2023): Januari
Publisher : Akademi Kesehatan Rustida

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55500/jikr.v10i1.191

Abstract

Escherichia coli yaitu bakteri yang hidup diusus manusia, sebagai flora normal. Akan tetapi bakteri ini juga sering mengkontaminasi dan dapat menyebabkan penyakit pada manusia. Pengolahan pangan yang tidak hygienis dapat menyebabkan berkembangnya bakteri ini. Penelitian ini bertujuan untuk mengidentifikasi adanya bakteri Coliform pada susu kedelai yang disimpan disuhu kulkas dan suhu ruangan metode MPN (Most Probable Number). penelitian ini bersifat deskriptif analitik dengan populasi susu kedelai yang disimpan disuhu kulkas dan suhu ruangan pada penyimpanan ½, 1, 1 ½, dan 2 hari. Berdasarkan hasil pengujian dengan metode presumptive test, confirmative test dan complete test didapatkan hasil pada penyimpanan disuhu kulkas penyimpanan ½ hari dan 1 hari sampel 1,2,3,4,5 yaitu <3 CFU/mL, penyimpanan 1 ½ hari sampel 1, 2 dan 3 didapatkan 15 CFU/mL, sampel 4 dan 5 yaitu 11 CFU/mL. Penyimpanan 2 hari didapatkan 20 CFU/mL. Sedangkan pada penyimpanan disuhu ruangan ½ hari sebesar <3 CFU/mL, penyimpanan 1 hari pada sampel 1,2 dan 3 yaitu 11 CFU/mL, sampel 4 dan 5 yaitu 7 CFU/mL. Penyimpanan 1 ½ hari didapatkan sampel 1,2 dan 3 yaitu 21 CFU/mL, sampel 4 dan 5 yaitu 20 CFU/mL, penyimpanan 2 hari yaitu 28 CFU/mL. Pada penyimpanan ½ hari dan 1 hari pada penyimpanan disuhu kulkas serta penyimpanan pada suhu ruangan ½ hari dinyatakan tidak ada pengaruh dan layak dikonsumsi serta memenuhi syarat mutu, Sedangkan pada penyimpanan 1 ½ hari dan 2 hari pada suhu kulkas dan 1, 1 ½, dan 2 hari pada suhu ruangan terdapat pengaruh penyimpanan dan tidak memenuhi syarat mutu konsumsi.
SEMINAR PEMERIKSAAN DENGAN METODE REAL TIME POLYMERASE CHAIN REACTION ASSAY (RT-PCR) SEBAGAI TES CEPAT MYCOBACTERIUM TUBERCULOSIS DARI SAMPEL DAHAK PASIEN TUBERCULOSIS DI PUSKESMAS LUBUK PAKAM Suventi Syafrina Ginting; Visensius Krisdianilo
JURNAL PENGMAS KESTRA (JPK) Vol 1 No 2 (2021): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (402.558 KB) | DOI: 10.35451/jpk.v1i2.633

Abstract

Mycobacterium tuberculosis is known as a highly pathogenic bacterium. These bacteria are aerobic in shape like rods and have resistance to acids. These bacteria can cause tuberculosis (TB). TB disease has been widely known as a fairly high cause of death in the world. Tuberculosis causes the death of nearly one million women each year. Currently not a single country in the world has been free of tuberculosis. Data shows that Indonesia is the third largest contributor to tuberculosis cases in the world. Examination of mycobacterium tuberculosis bacteria that are routinely performed in hospitals or health centers is using microscopic diagnosis of acid-resistant basil (BTA). Recently there has been a quick test of Mycobacterium tuberculosis using the semi-quantitative Real Time Polymerase Chain Reaction Assay (RT-PCR) method that targets the rpoB gene in Mycobacterium tuberculosis, which can automatically process preparations by extraction of doxyribo nucleic acid (DNA) in catridge. Rt-PCR test results are very specific in detecting Mycobacterium tuberculosis. There are some results that are not detected with microscopic examination (BTA) can be detected by RT-PCR technique. However, overall there is no significant difference in results between microscopic methods and RT-PCR. As well as seminar participants can follow and understand the materials submitted and also the examination procedures carried out.
EFEKTIVITAS SEDIAAN GEL ANTISEPTIK TANGAN EKSTRAK DAUN SIRSAK Annona muricata Linn SEBAGAI ANTIBAKTERI TERHADAP Staphylococcus epidermidis VINCENTIA ADE RIZKY; SAADAH SIREGAR; VISENSIUS KRISDIANILO; SITI KHADIJAH
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 9 No. 1 (2024): Bioma : Januari - Juni 2024
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Soursop leaf (Annona muricata Linn) has flavonoid, alkaloid, and saponin compounds that can be used as antibacterial. Based on the compounds contained in soursop leaves can be used as a hand antiseptic. Based on this study, an antiseptic gel formulation of soursop leaf (Annona muricata Linn) as antibacterial against Staphylococcus epidermidis bacteria with a carbomer 940 base was made. Antiseptic gel of soursop leaf extract was formulated with unequal extract concentrations, namely in the 1 3% formulation, the 2.6 % formulation. , formulation 3 9%, and formulation 4 12%. The antibacterial test used was the well diffusion method. The antiseptic gel was tested for its physical properties, namely organoleptic test, homogeneity test, dispersion test, and pH test. Antiseptic gel preparations that have antibacterial activity against Staphylococcus epidermidis bacteria are formulations 3 and 4 9% and 12% extracts which have a picture zone of 5 and 7 mm while the positive control has an inhibition zone of 12 mm. The results of the tests carried out on organoleptic tests, dispersion tests, pH tests, homogeneity tests were in accordance with the parameters of the gel preparation. From this study, it showed the ability of soursop leaves to inhibit the growth of Staphylococcus epidermidis bacteria. Soursop (Annona muricata Linn) leaf extract. Has antibacterial activity against Staphyloccus epidermidis bacteria with a concentration of 3% formulation 1, formulation 6% concentration 2, formulation concentration 9%, formulation concentration 12% 4. At a concentration of 3%, 6% has inhibitory power with a diameter of 3mm, and a concentration of 9% had an inhibitory power with an inhibitory diameter of 5mm, while the concentration of 12% had an inhibitory power with an inhibitory diameter of 7mm against the bacterium Staphyloccus epidermidis.
Co-Authors Asvia Rahayu Dian Pratiwi Morischa Deyla Sitanggang Neti Eka Jayanti Reh Malem br Karo Rinaldo Berutu SAADAH SIREGAR Sa’adah Siregar Sinurat, Jhon Patar SITI KHADIJAH Suci Mustika Mawardani Suventi Syafrina Ginting VINCENTIA ADE RIZKY Vincentia Ade Rizky