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All Journal Torani Journal of Fisheries and Marine Science Jurnal Perikanan Universitas Gadjah Mada Asian Journal of Agriculture Bioeksperimen Pedagogical Education and Review of Linguistics BIOTROPIC
Sriwulan Sriwulan
Fakultas Ilmu Kelautan Dan Perikanan, Jurusan Perikanan Universitas Hasanuddin
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Social Sciences

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DETEKSI VIRUS PENYEBAB PENYAKIT KERDIL PADA BENIH UDANG WINDU (Penaeus monodon) DENGAN MULTIPLEKS PCR Sriwulan Sriwulan; Hilal Anshary
Jurnal Perikanan Universitas Gadjah Mada Vol 13, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.995 KB) | DOI: 10.22146/jfs.3032

Abstract

Fenomena penyakit kerdil pada udang monodon yang umum dikenal sebagai monodon slow growth syndrome (MSGS) telah dilaporkan menyebabkan kerugian yang signifikan terhadap produksi udang di banyak negara termasuk Indonesia. Sindrom pertumbuhan yang lambat dari P. monodon disebabkan karena infeksi virus, monodon baculovirus (MBV), infection hypodermal and hematopoietic necrosis virus (IHHNV) dan hepatopancreatic parvovirus (HPV). Tujuan dari penelitian ini adalah untuk menganalisis terjadinya infeksi virus yang dikaitkan dengan gejala pertumbuhan lambat serta untuk mengukur prevalensi infeksi virus benih udang di Sulawesi Selatan, khususnya di Kabupaten Takalar, dengan menggunakan teknik deteksi multipleks PCR (MPCR). Teknik multipleks PCR merupakan teknik yang relatif baru yang digunakan untuk mendeteksi secara bersamaan lebih dari satu agen virus dalam satu tabung reaksi. Dalam penelitian ini, 3 pasang primer spesifik digunakan untuk proses amplifikasi IHHNV, MBV dan HPV. Setelah melakukan optimasi beberapa kali pada suhu annealing, deteksi virus ini dengan teknik MPCR pada benih udang menunjukkan bahwa ketiga virus, sebagai agen penyebab MSGS, ditemukan pada tempat penetasan yang diamati. Produk PCR IHHNV terbaca di 302 bp, MBV pada 261 bp dan HPV pada 595 bp. Prevalensi infeksi IHHNV, MBV dan HPV masing-masing adalah 50%, 62% dan 44%. Prevalensi dari ketiga virus dianggap tinggi dan bisa menjadi sumber penularan virus di tambak udang tanpa seleksi benih yang tepat.
TINGKAT INFEKSI PARASIT Thaparocleidus sp. PADA INSANG IKAN PATIN (Pangasianodon hypopthalmus) Hilal Anshary; Sriwulan Sriwulan; Junianto Talunga
Jurnal Perikanan Universitas Gadjah Mada Vol 15, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9101

Abstract

Monogenean is a parasite group which most often causes serious disease in fish due to its direct life cycle, high reproductive potential, and high pathogenicity. The aim of this study was to investigate infection level of Thaparocleidus sp.onstriped catfi sh which infected catfi sh fingerling. Striped catfi sh fi ngerling with 5–10 cm in total length samples from three diferent ornamental fish supplier in Makasar were  examined. Sampling was conducted weekly for three weeks and 10 fishes from each locations were collected. Samples were transported to laboratorium with aerated plactic bags and transported to Laboratory of Fish Parasite and disease for parasite examination. Organ which examined were gills. Prevalence of Thaparocleidus sp infection in all locations sampled was 100%. Mean intensity of the parasite infection was 98, 58 and 70 in location I, Location II, and Location III, respectively. The high prevalence and mean intensity of Thaparocleidus sp. infection indicated that health management in the aquaculture facilities was not properly implemented and this parasite existance is one of aquaculture problems to develop catfi sh fulfi lling ornamental or consumption demand .
The Utilization of Sweet Potatoes as Prebiotics on The Performance of Lactobacillus sp. in The Vanamei Shrimp Digestion (Litopenaeus vannamei) Theresia, Maria; Aslamyah, Siti; Sriwulan, Sriwulan
Torani Journal of Fisheries and Marine Science VOLUME 3 NOMOR 1, DESEMBER 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35911/torani.v3i1.7241

Abstract

The use of probiotics (Lactobacillus  sp.) in feed is one alternative that is done to improve immunostimulants, growth stimulants, and can be used as a balance of microorganisms in digestion. This study aimed to evaluate the growth performance of vanamei shrimp fed with the addition of Lactobacillus  sp. with sweet potatoes in feed. This study used a completely randomized design (CRD) with 4 treatments and 3 replications. The treatment was by feeding with different sweet potato concentrations: A) The dose of sweet potato in feed was 0% (as control); B) The dose of sweet potato in feed was 10%; C) 1The dose of sweet potato in feed was 15%; and D) The dose of sweet potato in feed was 20%. Data were analyzed using variance analysis (ANOVA) and continued with W-Tuckey's further test. The research parameters were digestive enzyme activity, nutrient digestibility, and growth. The results of the variance analysis showed the addition of various sweet potato concentrations with Lactobacillus sp. has no significant effect (p> 0.05) of the growth rate. The specific growth rate of vannamei shrimp ranges from 3.06 to 3.23% / day. Based on the results of the analysis on the utilization of sweet potatoes as a prebiotic on the performance of Lactobacillus sp. in the vannamei shrimps (Litopenaeus vannamei) digestive tract can be concluded that the growth performance does not have a significant effect after being fed with the addition of Lactobacillus sp. and sweet potatoes in feed. Keywords: Enzymes, Digestion, Growth, Lactobacillus sp., Prebiotic, Probiotic.
Penerapan Game Edukasi Word Wall untuk Meningkatkan Penguasaan Kosakata Bahasa Arab Sriwulan, Sriwulan; Kartini; Said, Rukman AR.; Hasnidar
Pedagogical Education and Review of Linguistics Volume 2 Issue 1, January 2026
Publisher : South Sulawesi Education Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58230/pearl.v2i1.324

Abstract

This study discusses how the application of word wall educational games to improve the mastery of Arabic vocabulary of class VIII students at the palopo city state tsanawiyyah madrasah. This research used pre-experimental method with one group pre-test and post-test design. Data collection was done through observation, test, and documentation. The research instruments were tested using validity and reliability tests to ensure the feasibility of the data. Data were analyzed using normality test, Wilcoxon test, and N-Gain test. The results showed a significant increase in students' mastery of Arabic vocabulary after the application of Word Wall. The mean score increased from 46 in the pre-test to 61.33 in the post-test. Wilcoxon test resulted in Asymp. Sig. (2-tailed) value of 0.003 <0.05, which indicates a significant difference between the pre-test and post-test scores. Based on the N-Gain test, the effectiveness of the Word Wall application was in the medium category with a score of 0.35 (35.46%). The application of Word Wall proved to be effective as a fun and innovative learning alternative to improve students' vocabulary mastery.
Eco-friendly control of Trichodinid infestation in Nile tilapia Oreochromis niloticus using Psidium guajava leaf extract SULISTIAWATI, ANDI; LAMATA, GUNARTO; SRIWULAN, SRIWULAN
Asian Journal of Agriculture Vol. 9 No. 2 (2025)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g090216

Abstract

Abstract. Sulistiawati A, Lamata G, Sriwulan. 2025. Eco-friendly control of Trichodinid infestation in Nile tilapia Oreochromis niloticus using Psidium guajava leaf extract. Asian J Agric 9: 498-506. Trichodinid ectoparasites are among the most detrimental pathogens in Nile tilapia (Oreochromis niloticus) aquaculture, causing tissue damage, growth suppression, and high mortality, particularly in small-scale systems. Chemical treatments such as formalin remain common but raise concerns regarding toxicity, environmental contamination, and pathogen resistance. Developing eco-friendly, plant-based alternatives is therefore crucial for sustainable aquaculture. This study evaluated the antiparasitic efficacy, safety, and histopathological impacts of ethanol extract of guava (Psidium guajava) leaves against Trichodinid infections in Nile tilapia. Juvenile tilapia (n=120) were naturally infected through cohabitation and randomly assigned to four treatments (0, 10, 20, and 30 ppm extract; three replicates each). Acute toxicity tests determined a 24 h LC50 value of 43.65 ppm. Extract efficacy was assessed through prevalence, infection intensity, survival rate, water quality, and histopathology, analyzed by one-way ANOVA with Tukey’s post hoc test (?=0.05). The 30 ppm treatment significantly reduced Trichodinid prevalence from 70.0% to 43.3% and infection intensity from 5.4 to 3.2 parasites per fish, representing 38.1% and 41.3% reductions, respectively (p<0.05). The highest survival rate occurred at 10 ppm (93.3%), compared to 86.7% at 20 ppm, 80.0% at 30 ppm, and 66.7% in the control. Water quality (temperature, dissolved oxygen, pH) remained within optimal culture ranges. Histopathological observations confirmed milder epithelial erosion, necrosis, and gill lamella fusion in treated groups relative to controls. These findings demonstrate that guava leaf ethanol extract, particularly at 10-30 ppm, is an effective, safe, and environmentally friendly antiparasitic agent. Its integration into aquaculture health management could reduce reliance on synthetic chemicals, promote fish welfare, and support the sustainability of tilapia farming systems.
In vitro antifungal activity of Mimosa pudica rhizosphere bacteria against Fusarium spp. Sriwulan, Sriwulan; Mustikaningrum, Dhina; Nurfitria, Nia
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.13509

Abstract

This study aims to explore rhizosphere bacteria from Mimosa pudica L. as a source of antagonistic bacteria against Fusarium spp. Rhizosphere soil samples were collected, isolated, morphologically characterized, and tested for antifungal activity in vitro against Fusarium spp. The percentage of inhibition (PI) was calculated after 7 days. Six bacterial isolates were obtained and tentatively identified as Bacillus, Cellulomonas, Arthrobacter, and Micrococcus species. All isolates exhibited antagonistic activity against Fusarium spp., with inhibition percentages ranging from 61.99% to 81.83%. Isolate PM2 (tentatively identified as Cellulomonas) demonstrated the strongest inhibition at 81.83%, followed by isolate PM6 (tentatively identified as Micrococcus) at 79.25%. These results confirm the potential of Mimosa pudica L. as a source of biocontrol agents and warrant further investigation into their molecular identification, mechanisms of action, and in vivo efficacy.
Profil Fitokimia dan Aktivitas Antibakteri Kombucha Mesocarp Siwalan terhadap Propionibacterium acnes Sejati, Wahyu Sukma; Sriwulan, Sriwulan; Nurfitria, Nia
Biotropic : The Journal of Tropical Biology Vol. 9 No. 2 (2025): Biotropic, Volume 9 Nomor 2, 2025
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v9i2.2352

Abstract

Kombucha is a fermented probiotic drink made from sugar with the help of Symbiotic Consortium of Bacteria and Yeast (SCOBY). This study aims to determine the phytochemical profile and antibacterial activity of kombucha of Palmyra palm mesocarp (Borassus flabellifer) against Propionibacterium acnes, the main cause of skin acne. The research method used a complete randomised design (CRD) with 7 and 14 days of fermentation. Phytochemical test was conducted qualitatively to identify flavonoids, tannins, saponins, and alkaloid compounds. Meanwhile, the disc diffusion method was used to test the antibacterial activity of Palmyra palm mesocarp kombucha against P. acnes. The results showed that Palmyra palm mesocarp kombucha contained all the phytochemical compounds tested, with flavonoids only detected at 14 days fermentation and the alkaloid content was higher at 14 days fermentation. The highest antibacterial activity was obtained in 14-day fermented kombucha with an average inhibition zone diameter of 36.9 mm and categorized as very strong, equivalent to the positive control in the form of chloramphenicol. The flavonoid and alkaloid contents is thought to play a role in inhibiting bacterial growth. Thus, kombucha of Palmyra palm mesocarp has the potential as a natural antibacterial agent in the treatment of acne caused by P. acnes.  
Co-Authors Dhina Mustikaningrum Hasnidar Hilal Anshary Hilal Anshary Junianto Talunga KARTINI LAMATA, GUNARTO Nia Nurfitria Said, Rukman AR. Sejati, Wahyu Sukma Siti Aslamyah Sulistiawati, Andi Theresia, Maria