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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner JURNAL KAJIAN VETERINER
Mirnawati Bachrum Sudarwanto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health Veterinary

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Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga Campus Aminudin, Muhammad Rizki; Lukman, Denny Widaya; Sudarwanto, Mirnawati Bachrum; Pisestyani, Herwin
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.99843

Abstract

Several human and animal pathogens transmit into the food chain through houseflies as mechanical vectors, one of which is E. coli O157:H7. E. coli O157:H7 can express Shiga toxin (Stx) which can cause diarrhea, hemorrhagic colitis, and potentially fatal hemolytic-uremic syndrome (HUS) in humans. Some pathogen strains show resistance against various antibiotics, causing complex health problems. This study aims to analyze the presence of antibiotic-resistant E. coli O157:H7 bacteria carried by houseflies (M. domestica) in the food court IPB Dramaga campus area. Detection of E. coli O157:H7 on fly legs using qPCR method based on MU 7.2.3.32-8. E. coli O157:H7 isolates were tested for sensitivity to the antibiotic’s ciprofloxacin, ampicillin, tetracycline, trimethoprim-sulfamethoxazole, cefepime, chloramphenicol, ceftazidime, cefotaxime, and ceftriaxone using the Kirby Bauer disc diffusion method. This study isolated 5 E. coli isolates (5/40; 12.5%), and 2 of them were confirmed as E. coli O157:H7. One isolate of E. coli O157:H7 was resistant against ampicillin and tetracycline, and one isolate was resistant against ampicillin, tetracycline, trimethoprim-sulfamethoxazole, and ceftazidime. The multi-drug resistance was identified only in 1 isolate of E. coli O157:H7.Houseflies collected from the food court have the potential to transmit antibiotic-resistant E. coli O157:H7 around the IPB campus.
Multidrug-Resistant (MDR) E. coli dalam Daging Babi yang Berasal dari Rumah Potong Hewan (RPH) Oeba, Kota Kupang Mila Meha, Margie Pristiantine; Sudarwanto, Mirnawati Bachrum; Pisestyani, Herwin
Acta VETERINARIA Indonesiana Vol. 12 No. 2 (2024): Juli 2024
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.12.2.168-175

Abstract

Untuk dapat menghasilkan daging babi yang siap dikonsumsi tentunya perlu melewati berbagai tahapan mulai dari peternakan hingga pengemasan. Pada berbagai tahapan ini, peluang terjadinya kontaminasi dengan mikrob patogen sangat besar. Mikrob patogen yang sering ditemui pada daging babi salah satunya E. coli yang seringkali menjadi pemicu kejadian foodborne disease pada manusia. Penelitian sebelumnya menunjukkan bahwa isolat E. coli yang diisolasi dari peternakan babi di Kota Kupang diketahui telah mengalami multidrug-resistan (MDR) yang mana apabila kejadian ini menyebar dapat berdampak buruk bagi masyarakat. RPH merupakan tempat penyembelihan hewan yang diawasi oleh dokter hewan dengan tujuan untuk dapat menghasilkan daging dengan kualitas baik bagi masyarakat. Penelitian ini bertujuan untuk mengukur cemaran E. coli, mengidentifikasi serta isolasi MDR E. coli dari daging babi segar yang berasal dari RPH Oeba, Kota Kupang. Penghitungan jumlah E. coli dilakukan berdasarkan Standar Nasional Indonesia (SNI) ISO 16649–2:2001 tentang Metode Horizontal Enumerasi E. coli positif-b-glukuronidase - Bagian 2: Teknik penghitungan koloni pada suhu 44 °C menggunakan 5-bromo-4-chloro-3-indolyl-b-D-glucuronide. Uji resistansi E. coliterhadap antibiotik dilakukan berdasarkan metode Kirby-Bauer. Data terkait jumlah cemaran E. coli dan pengujian resistansi E. coli terhadap antibiotik dianalisis secara deskriptif. Hasil penelitian menunjukkan cemaran E. coli dalam daging babi yang digunakan memiliki rata-rata 626.440±947.937 CFU/g. Semua isolat E. coli telah resistan terhadap penisilin (100%), sebagian besar isolat E. coli resistan terhadap streptomisin (80%) dan tetrasiklin (60%), serta hampir sebagian isolat E. coli resistan terhadap oksitetrasiklin (47%), amoksisilin (40%). Isolat lapang E. coli yang diperoleh dari daging babi telah MDR terhadap 2 (7/15;46,67%) sampai dengan 3 (7/15;46,67%) golongan antibiotik. Simpulan dari penelitian ini yaitu, cemaran E. coli dalam daging babi yang berasal dari RPH Oeba melebihi ambang batas maksimum cemaran mikrob yang telah ditetapkan (>1x101 CFU/g). Isolat E. coli telah resistan terhadap penisilin, streptomisin, tetrasiklin, oksitetrasiklin, dan amoksisilin dan telah MDR terhadap 2 sampai dengan 3 golongan antibiotik.
Analisis Fisikokimia dan Aktivitas Antibakteri Madu Hutan Timor terhadap Methicillin-Resistant Staphylococcus aureus Undari, Fajar Okta; Latif, Hadri; Sudarwanto, Mirnawati Bachrum
JURNAL KAJIAN VETERINER Vol 13 No 1 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i1.21448

Abstract

The emergence of antibiotic-resistant bacteria has become a global concern. One of the bacteria that is resistant to several types of antibiotics is Methicillin-Resistant Staphylococcus aureus (MRSA). Honey is known for its nutritional and antibacterial properties. The antibacterial activity of honey is due to several factors such as its physicochemical properties, H2O2, and phytochemicals compounds. Honey could be a promising alternative to overcome the problem of antibiotic resistance. The aim of this research was to determine the physicochemical, H2O2, Pinocembrin, Kaempferol and antimicrobial activity of Timor Forest honey. A total of 7 Timor Forest honey was analyzed to determine water content, water activity, pH, H2O2, Pinocembrin, Kaempferol and antibacterial activity. Moisture content, water activity, and pH were measured using a refractometer, aw-meter, and pH meter, respectively. Hydrogen peroxide content was measured using peroxide strip test. Pinocembrin and Kaempferol contents were measured using HPLC-DAD. The analysis showed that the water content, water activity and pH of Timor Forest honey were 17.52±3.31%, 0.6±0.01, 4.18±0.32, respectively. The content of H2O2, Pinocembrin, and Kaempferol were 109.1±47.1 µmol/L, 2.2±1.9 mg/100 g, 1.7±0.7 mg/100 g, respectively. The antibacterial activity of Timor Forest honey against MRSA was tested using Kirby-Bauer disc diffusion method. Vancomycin was used as positive control. The inhibition zone of Timor Forest honey and vancomycin was not significantly different with an average of 13.93±0.23 mm for Timor Forest honey and 14.33±0.05 mm for vancomycin. Timor forest honey has good physicochemical properties with a relatively high content of Pinocembrin and Kaempferol and has antibacterial activity against MRSA.
Co-Authors Aminudin, Muhammad Rizki Denny Widaya Lukman Faisal Tanjung Hadri Latif HERA MAHESHWARI Herwin Pisestyani Idwan Sudirman Mila Meha, Margie Pristiantine Ratmawati Malaka Undari, Fajar Okta Wahniyathi Hatta