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All Journal JURNAL ANATOMI FISIOLOGI PIKSEL : Penelitian Ilmu Komputer Sistem Embedded and Logic Jurnal Surya Medika Animal Production : Indonesian Journal of Animal Production Insect (Informatics and Security): Jurnal Teknik Informatika KOPERTIP: Jurnal Ilmiah Manajemen Informatika dan Komputer NUCLEUS: Jurnal Sains dan Teknologi Jurnal ABDIMAS (Pengabdian kepada Masyarakat) UBJ Journal of Students‘ Research in Computer Science (JSRCS) Jurnal Sains Teknologi dan Sistem Informasi Fahma : Jurnal Informatika Komputer, Bisnis dan Manajemen
Sugiyatno Sugiyatno
Lab. BSF Tumbuhan, Jurusan Biologi, FSM, Undip Semarang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Veterinary

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Journal : Animal Production : Indonesian Journal of Animal Production

 1 
Effect of Number of Spermatozoa, Oviduct Condition and Timing of Artificial Insemination on Fertility and Fertile Period of Kampung Rooster Spermatozoa Saleh, DM; Sugiyatno, Sugiyatno; Mugiyono, S; Siswadi, RW
ANIMAL PRODUCTION Vol 14, No 1 (2012): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract. This study was carried out to determine the optimum fertility and fertile period using the number of spermatozoa, oviduct condition and timing of insemination of native rooster spermatozoa. Ninety six commercial Isa brown pullets and nine kampung roosters were used in this study in a 3×2×2 factorial arrangement with one bird in a cage constituting a unit. The factor levels were the number of spermatozoa (50, 100 and 150 million/0.1 ml), oviduct condition (hard-shelled eggs and free hard-shelled eggs), and timing of artificial insemination (in the morning, at 7 AM and in the afternoon, at 4 PM).  The results showed that among the treatments there was no significant interaction to fertility and fertile period. Insemination with 50 million sperm number seemed to be the same result with the other 2 treatments. Oviduct condition had a highly significant difference on fertility and fertile period percentage, and timing of insemination did not differ between morning and afternoon.  In conclusion, the only oviduct condition (free hard-shelled eggs) was the best results for insemination in terms of fertility and fertile period of native roosters.  It is recommended that for the maximum fertility and fertile period, hens should be inseminated with 50 million spermatozoa, free of hard-shelled eggs and insemination performed in the morning or in the afternoon. Keywords: timing of artificial insemination, fertility, fertile period, semen dose, oviduct conditionAnimal Production 14(1):32-36, January 2012
Quality Of Pe Buck Sperm Stored In Different Packaging Pangestu, M; Soenarjo, CH; Siswadi, Rachmawati W; Sugiyatno, Sugiyatno; Tagama, Taswin R; Saleh, Dadang M; Haryati, Haryati; Sutarmo, Sutarmo; Setyawati, SJA
ANIMAL PRODUCTION Vol 1, No 1 (1999): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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AI for cattle has been develop in Indonesia in contrast, AI for small ruminants (sheep and goat) are less developed. Its due to the lack of facilities, processing and packaging during storage. This research aims to compare the effectivity and efficiency of two different packaging technique (test tube and straw). Test tube and 0.25 ml mini straw were tested . Semen was obtained from adult PE buck (3 yr) after collection by using  artificial vagina. The fresh semen was then evaluated and diluted 5 folds with 2.9 percent Na-citrate. Diluted semen then packed in test tube and mini straw, and stored in refrigerator (100C) for 7 days. Observations were done everyday on sperm motility, abnormality and percentage of  live sperm. Observation were made at 370C. Observation on fresh ejaculate showed that semen has 6x 109/ml concentration, 90 percent motility, 8 percent abnormality and  95 percent  live sperm. Five folds dilution reduced sperm concentration to 1.2 x 109/ml, but did not change sperm motility, abnormality and percent of live sperm. Sperm was then packed according  to the treatments. Storage in both packaging did significantly reduce  (P<0.01) sperm motility and percent live sperm, but not for sperm abnormality. All sperm stored in test tube were classified death at day 5 storage. However, sperm in the straw were remain live eventhough the percentages was low. It can be concluded that straw has a better result than test tube. Eventhough sperm motility was extremely low, it remains valuable for cervical insemination. (Animal Production 1(1) : 24-29 (1999).  Key Words : Sperm, PE Buck, Straw, Test Tube
Serum Estrogen Concentration in Relation to Estrous Duration of Thin-tail Ewes with Inducted of PMSG Sugiyatno, Sugiyatno; Sumaryadi, MS; Haryati, Haryati
ANIMAL PRODUCTION Vol 3, No 1 (2001): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Fifteen thin tail ewes were used in to study of contribution of maternal serum estrogen concentration this in relation to estrous duration with inducted of PMSG. The first step, all experiment ewes were injected prostaglandin (7.5 mg luprositol per head)  i.m. as much twice of interval day 11 to homogeneity follicle growth phase. In the estrous observed was estrous (700 IU duration, then blood sampling taken out of vena jugulars to analyses of estrogen. The second step, all experiment  ewes were inducted PMSG  (700 IU per head), then in the estrous observed was same as the first step. The result of experiment show that induction of PMSG  influence to  maternal serum estrogen concentration has different significantly (P<0.01)and estrous duration has different significant (P<0.05).Maternal serum estrogen concentration prior and post of  induction of PMSG contributed 25.28 and 57.12 percent, respectively. It was concluded that the estrous duration in relation  to increased maternal serum estrogen concentration. (Animal Production 3(1): 40-44 (2001) Key Words: Estrogen, estrous, ewes
Effect of Artificial Insemination Time on the Fertility of Layer Hens Saleh, DM; Sugiyatno, Sugiyatno
ANIMAL PRODUCTION Vol 8, No 2 (2006): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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A study on the effect of AI time on the fertility of layer hens was carried out. Semen was collected from eight kampung roosters, with age ranging from 12-18 months.  Completely Randomized Design was used to analyze the data obtained.  Every eight hens (n=8) was inseminated intravaginally at 0-2 hrs, >2-4 hrs, > 4-6 hrs and >6-8 hrs after oviposition.  The results showed that insemination time did not affect  (P>0.05) the percentage of fertility from days 2 to 20.  However, the insemination time affected (P<0.05) the percentage of fertile eggs from days 2 to 9 and it did not affect (P>0.05) the mean duration of the fertile period.  In conclusion, the time of insemination at 0 – 2 hours after oviposition gave the best fertility.  It is recommended that for the best fertility flock inseminations should be performed at a time of 0-2 hours after an egg is laid every 9 days. (Animal Production 8(2): 83-87 (2006) Key Words: Kampung roosters, semen, fertility, fertile period, AI time
Effect of Glycerol Levels on the Motility and Fertility of Kampung Chicken Spermatozoa Frozen in Liquid Nitrogen Saleh, DM; Sugiyatno, Sugiyatno
ANIMAL PRODUCTION Vol 9, No 1 (2007): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The use of various levels of glycerol as a cryoprotective agent for freezing kampung chicken semen was examined with respect to intravaginal insemination.  There was a significant (P<0.05) effect of various levels of glycerol (4, 8, 12 and 16 %) on motile spermatozoa, but there was no significant effect on fertility.  It was concluded that the use of glycerol for preservation of kampung chicken semen in liquid nitrogen gave satisfactory cryopreservative results on    motility of thawed kampung chicken spermatozoa, but failed to produce fertile eggs. (Animal Production 9(1): 45-48 (2007) Key Words: glycerol, kampung chicken, semen, fertility, motility, liquid nitrogen
Co-Authors Afri Wahyuningsih Akmalullah, Farigh Mukhlashin CH Soenarjo, CH Dadang M Saleh, Dadang M Dadang Mulyadi Saleh Didik Setiyadi DM Saleh, DM Erma Prihastanti Fata Nidaul Khasanah Fathurrozi , Ahmad Haryati Haryati Herlawati Herlawati Ibrahim Fikma Edrisy Juliano, Aryo M Pangestu, M MS Sumaryadi, MS Munifatul Izzati Noviar Harun Prabandari, Rosiana Disiati Prima Dina Atika Purnomo , Rakhmat Rachmawati W Siswadi, Rachmawati W Rafika Sari Rahmadya Trias Handayanto Rasim, Rasim RW Siswadi, RW S Mugiyono, S Seta Samsiana Sidiq, Purnomo SJA Setyawati, SJA Suroso, Amat Sutarmo Sutarmo, Sutarmo Taswin R Tagama, Taswin R