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Callus Induction and Plant Regeneration of Brachiaria Grass from Immature Inflorescence Explants Nilo Suseno; Nofi Isnaini; Bambang Suwignyo; Bambang Suhartanto; Nafiatul Umami
Buletin Peternakan Vol 45, No 2 (2021): BULETIN PETERNAKAN VOL. 45 (2) MAY 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i2.43461

Abstract

This research was aimed to optimize concentration of phytohormone in callus induction and in vitro regeneration of three species of Brachiaria grass plant, namely Brachiaria brizantha, B. decumbens, and B. ruziziensis. Immature inflorescences were used as explant material. To induce callus, explants were inoculated into Murashige and Skoog (MS) basal medium supplemented with phytohormone combination of 2,4-dichlorophenoxyacetic acid (2,4-D), dicamba and kinetin. Observation of callus induction included percentage of callus formation and callus morphology. The embryogenic calli were then transferred into regeneration media, i.e. MS basal medium supplemented with kinetin and 6-benzylaminopurin (BAP). The result showed that 4 mg/L 2,4-D + 0.2 mg/L kinetin showed highest callus induction in B. brizantha and B. decumbens, namely  76%  and 88% respectively. Whereas in B. ruziziensis, 3 mg/L 2,4-D + 0.2 mg/L kinetin showed highest callus induction, namely 86%. MS medium supplemented with 4 mg/L kinetin showed highest regeneration in all three grass species, namely 92.5% in both B. brizantha and B. ruziziensis, and 88.75% in B. decumbens.
Effect of Organic and Inorganic Fertilizers on Yield and Quality of Synedrella nodiflora (Tropical Weed) Bambang Suwignyo; Galih Pawening; Muhammad Humaidi Haris; Nafiatul Umami; Nilo Suseno; Bambang Suhartanto
Buletin Peternakan Vol 44, No 4 (2020): BULETIN PETERNAKAN VOL. 44 (4) NOVEMBER 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i4.56115

Abstract

This study aimed to determine the effect of organic and inorganic fertilizers on the cultivation of tropical weed Synedrella nodiflora as forage. The study was conducted from May to July 2018. The treatments of the research were control (C), organic fertilizer (D) and organic fertilizer + urea (DU), with 3 replications that were arranged on experimental design with a completely randomized design, in a unidirectional pattern and continued with least significant different (LSD). Organic fertilizer dosage in this study was 5 tons/ha, while urea fertilizer was 350 kg/ha, with plant spacing was 45x60 cm. The observed parameters were plant height, forage production and chemical composition (5 weeks after planting). Plant height of C, D and DU were 41.59, 47,42, and 50.59 cm respectively. Forage production of dry matter after 5 weeks planting at C, D and DU were 1.69, 1.70 and 2.91ton/ha, with in vitro digestibility values ranging from 51.68 to 57.70% (IVDMD) and 51.71 to 61.98% (OMD) respectively. The chemical composition of native S. Nodiflora were 12.32% of dry matter (DM), 62.45% TDN count for cattle and 67.42% TDN count for sheep. Based on DM, The organic matter was 84.46%, crude protein 20,11%, crude fiber 13.26%, extract ether 7.77%, and nitrogen free extract 37.08%. The combination of organic fertilizer and urea increased the height and fresh and dry matter production S. nodiflora.
PENAMBAHAN INOKULUM DALAM MENINGKATKAN KUALITAS JERAMI KEDELAI EDAMAME (Glycine max var Ryokhoho) SEBAGAI PAKAN TERNAK Nafiatul Umami; Heny Marlina Wijayanti; Dyah Afryana Miftah Nurdani; Ristianto Utomo; R. Djoko Soetrisno; Bambang Suhartanto; Bambang Suwignyo; Cahyo Wulandari
Pastura : Jurnal Ilmu Tumbuhan Pakan Ternak Vol 2 No 1
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.338 KB) | DOI: 10.24843/Pastura.2012.v02.i01.p06

Abstract

This research was conducted to investigated the effect of rhizobium inoculation and harvesting time on the productivity of edamame and the chemical composition in the straw edamame. This study was planted edamame soy bean seed. This experiment was carried out in green house used regosol soil in polybag, 2x2 factorial experiment with five replication was arranged in completely randomized design, continued by Duncan’s multiple range test (DMRT) for the significant result. The first factor was harvesting time (U) consisting of harvested at 65 days (U1) and harvested at 75 days (U2); the second factor was inoculant (I) consisting of with inoculation (I1) and without inoculant (I0). The result of the study showed, that underground dry weight (DW) mass yield and DM and OM straw productions, crude protein (CP), nitrogen free extract (NFE) of UP1 were higher (P<0.01) and ash of straw were higher(P<0.05) than UP2. DM and OM pod productions of UP1 was also superior (P<0.05) than UP2. UP2 resulted better fiber crude (FC) (P<0.01) than UP1. Inoculation (L1) resulted better DM and OM straw productions and CP of straw (P<0.01), and underground DW mass yield, FC, NFE of straw were affected (P<0.05) by interaction between treatments. Underground DW mass yield, FC, NFE, DM and OM of straw, and pods productions were not affected by interaction. And all the treatments were also not affected ether extract (EE) of straw.The results of experiment shown that legin factor not signification of in vitro digestibility. The harvest time (U1) was higher (P<0.05) than harvested at 75 days. Interaction among two factors not significant on in vitro organic matter digestibility.
Penggunaan Fermentasi Pakan Komplet Berbasis Hijauan Pakan dan Jerami Untuk Pakan Ruminansia Bambang Suwignyo; Ali Agus; Ristianto Utomo; Nafiatul Umami; Bambang Suhartanto; Cahyo Wulandari
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 1, No 2 (2016): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.047 KB) | DOI: 10.22146/jpkm.10611

Abstract

Fermented complete feed (CF) based forage-fodder and agricultural by product as feed for ruminants can be implemented in the household and industrial purpose. Complete feed technology is one of alternative to solve the problems of stock and quality of feed. The nutritional value can be set by determine the number and type of mixture, livestock will not has an opportunity to choose so that it can minimize residual feed, practical, and can be stored for long periods. Fermented complete feed very much match to be implemented in the dry season or in the emergency situation. During Merapi Volcano eruption in 2010 was one of moment which is need CF technology. Rice straw can used for CF that can be socked during dry season, also the quality might improve (from 3—4% of crude protein content became 7—8%) with addition of rice brand or other ingredients. Fermented complete feed based on rice straw, forage-fodder or agricultural by product has a multifungtion in the emergency situation, reduce disaster risk, and feed security conservation.
Pengembangan Sistem Pertanian Siklus-Bio Terpadu untuk Peningkatan Produktivitas Ternak Sapi pada Kelompok Ternak Desa Margoagung, Sayegan, Sleman, Yogyakarta Cahyono Agus Dwikoranto; Ali Agus; Bambang Suhartanto
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 1, No 1 (2015): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (366.666 KB) | DOI: 10.22146/jpkm.16957

Abstract

Integrated Bio-Cycle Farming System was developed by KP4 University Farm UGM Yogyakarta should be implemented to the communities. The community service for implementation of science and technology starting with an MOU between KP4 and the government of Margoagung village, especially Farmer Group RUKUN, Margoagung, Seyegan, Sleman that requiring transferred technology. Community service programs and assistance through the transfer of integrated bio-cycle farming system were done through development of burgers feed technology for cows, solid fertilizer, liquid fertilizer, and waste treatment technology for biogas. This activity also involves 22 students from various faculties at UGM for 2 months in the field. The technology is transferred directly by experts from GMU and directly accompanied intensively by student. Enthusiastic, productivity, quantity, quality and continuity of integrated farming were very important for a better life and environment. 
Pengaruh Dosis Pupuk Urea dan Umur Panen terhadap Hasil Hijauan Sorgum (Sorghum bicolor (L) Moench) Dian Astuti; Bambang Suhartanto; Nafiatul Umami; Ali Agus
Agrotechnology Innovation (Agrinova) Vol 1, No 2 (2018)
Publisher : Agrotechnology Innovation Center, Universitas Gadjah Mada, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (628.355 KB) | DOI: 10.22146/agrinova.49134

Abstract

Penelitian dilakukan untuk mengetahui pertumbuhan dan produktivitas tanaman sorgum yang ditanam dengan umur panen dan dosis pemupukan urea yang berbeda. Penelitian dilakukan di lahan Pusat Inovasi Agroteknologi (PIAT) UGM selama 6 bulan, dengan desain RPPT (split split plot design) dengan menggunakan 3 faktor yaitu varietas (V) sebagai petak utama terdiri dari dua varietas yaitu V1 = Varietas Numbu dan V2 = Varietas BMR. Umur panen (U) sebagai anak petak terdiri dari 3 umur panen yaitu 50 hari (U1), 60 hari (U2) dan 70 hari (U3). Aras pupuk urea sebagai anak anak petak terdiri dari 3 aras yaitu 0 (P1), 100 (P2) dan 200 kg/ha (P3). Hasil penelitian menunjukkan bahwa umur panen (U) berpengaruh nyata (P<0,05) terhadap tinggi tanaman, hasil segar, hasil bahan kering dan hasil bahan organik. Dosis pemupukan urea berpengaruh nyata (P<0,05) terhadap tinggi tanaman, hasil segar, bahan kering dan hasil bahan organik  Perlakuan umur panen 70 hari dengan pemupukan Urea 200 kg/ha (U3P3) menunjukkan nilai tertinggi pada semua variabel.
Pengaruh Umur Panen dan Level Pupuk Nitrogen Terhadap Produksi dan Kandungan Nutrien Sorghum bicolor L. Varietas Numbu Dian Astuti; Bambang Suhartanto; Bambang Suwignyo; Melvin Zaenul Asyiqin
Agrotechnology Innovation (Agrinova) Vol 2, No 2 (2019)
Publisher : Agrotechnology Innovation Center, Universitas Gadjah Mada, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (615.455 KB) | DOI: 10.22146/agrinova.54702

Abstract

Sorghum is a potential feed crop to be developed in the tropics that has a long dry season such as in Indonesia. This study aims to determine the effect of harvesting age and nitrogen fertilizer level on the production and nutrient content of sorghum plants Numbu varieties. This study used a split block design with 2 factors and the difference between averages was tested with Duncan's Multiple Range Test (DMRT). The main factors are the age of harvest time consisting of U1 (50 days), U2 (60 days) and U3 (70 days). The second factor is the nitrogen fertilizer level which consists of P1 (0 kg / ha), P2 (100 kg / ha), and P3 (200 kg / ha). Each treatment was carried out 3 replications. The results showed that growth and production were influenced by harvest age and fertilizer level. The nutrient content of sorghum plants of Numbu variety which is influenced by harvest age is only BK, BO and SK, the contents of PK and LK, are not affected by harvest age. The highest BK content in treatment U3 (22.55%). The highest BO content in U3 treatment (91.90%). The highest SK content was in treatment U3 (38.56%). The content of PK and SK was significantly influenced by the nitrogen fertilizer (P <0.05), while the content of BK, BO and LK, was not significantly different. The highest PK content in treatment P3 (7.58%). The lowest SK content was P3 treatment (34.78%). These results indicate that the use of nitrogen fertilizers up to 200 kg / ha can increase (P <0.05) crude protein content, and reduce crude fiber content. 
Productivity of Butterfly Pea (Clitoria ternatea L.) Influenced by Urea Fertilizer Rates and Harvest Ages in Kulon Progo, Yogyakarta, Indonesia Wardi Wardi; Nafiatul Umami; Asih Kurniawati; Bambang Suhartanto; Chusnul Hanim; Zen Adyatama
ANIMAL PRODUCTION Vol. 25 No. 1 (2023)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2023.25.1.187

Abstract

Butterfly pea (Clitoria ternatea L.) is leguminous species that contain high nutritional values. This study aimed to determine the effect of the rate of urea fertilizer and harvest age on the productivity of butterfly pea forage. This research used a split-plot design consisting of urea rates (0, 100, and 200 kg/ha) and harvest ages (30, 45, and 60 days after planting or DAP) with three replications. The variables observed were morphological growth (plant height, stem diameter, number of leaves, and number of branches), and productivity of forage biomass (fresh weight, dry weight, and crude protein). The data obtained were analyzed using ANOVA and the means were separated by using Duncan's Multiple Range Test (DMRT). The interaction between fertilizer rates and harvest ages was shown (p<0.05) on stem diameter, number of leaves, number of branches, and fresh weight of butterfly pea. The increased rates of fertilizer and harvest ages significantly affected (p<0.05) in the morphological growth and productivity of butterfly pea biomass. The application of urea fertilizer increased plant length from 10.59 to 17.16%, stem diameter from 12.12 to 24.24%, number of leaves from 15.40 to 28.20%, and number of branches from 81.82 to 190.91% compared to control. It was concluded that the morphological growth and productivity of forage biomass increased with fertilizer rates and harvest ages. Treatment of 200 kg/ha urea and harvest age of 60 DAP produced the highest morphological and productivity.
PENGARUH BERBAGAI MACAM MEDIA TANAM POT ORGANIK TERHADAP PRODUKSI TANAMAN KACANG TUNGGAK (Vigna unguiculata) DI LAHAN BERPASIR Fitria Gemma Tyasari Bambang Suhartanto Bambang Suwignyo
AGRONOMIKA Vol 12 No 02 (2017): AGRONOMIKA Vol. 12 No. 2 Agustus 2017 – Januari 2018
Publisher : Universitas Islam Batik Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk mengetahui pertumbuhan dan produktivitas kacang tunggak yang ditanam di lahan pasir dengan perbaikan lingkungan tumbuh melalui penggunaan pot organik sebagai media tanam. Tiga blok lahan pasir (sebagai ulangan) ditanami kacang tunggak dengan menggunakan 5 macam pot organik sebagai media tanam terbuat dari kompos kotoran ayam, kambing, dan sapi, sludge biogas, dan sampah organik, serta kontrol (tanpa pot). Tiap blok lahan pasir dibagi menjadi 6 plot secara acak sesuai dengan perlakuan macam pot organik. Lima buah pot organik ditanam dalam tiap plot yang telah diisi tanah kemudian ditanam 2 biji kacang tunggak. Pemanenan dilakukan pada minggu ke-8, produksi hijauan ditimbang pada saat akhir penelitian Data dianalisis dengan analisis variansi menurut rancangan blok acak lengkap dan perbedaan rerata diuji Duncan’s. Hasil penelitian menunjukkan bahwa macam pot organik tidak berpengaruh nyata terhadap produksi tanaman. Lahan di blok II memiliki pengaruh lebih baik terhadap tinggi tanaman (58,20 cm), produksi bahan kering (41,23 g), produksi bahan organik (39,18g), dan produksi protein (5,65 g) dibanding blok lahan yang lainnya. Dapat disimpulkan bahwa media tanam pot organik ayam lebih baik dibandingkan pot organik lainnya. Penggunaan media tanam pot organik pada lahan pasir akan memperbaiki pertumbuhan dan produktivitas tanaman kacang tunggak.
The ruminant livestock industry EFFECT OF TYPE AND DOSAGE OF FOLIAR FERTILIZERS ON DIGESTIBILITY AND NUTRIENT CONTENT OF BUTTERFLY PEA (Clitoria ternatea): PENGARUH JENIS DAN DOSIS PUPUK DAUN TERHADAP KECERNAAN DAN KANDUNGAN NUTRIEN KEMBANG TELANG (Clitoria ternatea) Sariffudin, Apriyani Nur; Umami, Nafiatul; Suhartanto, Bambang; Ahmad, SN
Jurnal Peternakan Nusantara Vol. 10 No. 1 (2024)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jpn.v10i1.11377

Abstract

The study aimed to determine the effect of the type and dose level of foliar fertilizers on the nutrient content and in vitro digestibility of Clitoria ternatea. The research was conducted at Faculty of Animal Science, Gadjah Mada University. The materials used were butterfly pea seeds, rabbit liquid organic and Gandasil®D fertilizer, rumen fluid. The study analysed by Completely Randomized Design (CRD) with factorial 2x4. The first factor was the type of foliar fertilizer (rabbit liquid organic and Gandasil®D fertilizer). The second factor was the level of fertilizer dosage (0, 1.5, 3 and 4.5 g/l/plot). Parameters observed were DM, OM, Ash, CP, CF, Crude Fat, in vitro dry matter and organic matter digestibility (IVDMD/IVOMD). Data were analyzed by variance analysis at 5%, continued with Duncan's Multiple Range Test (DMRT). The results showed that the types of foliar fertilizers didn’t significantly affect the nutrient content (P>0.05), but had a significant effect on IVDMD and IVOMD (P<0.05). The dose level had a significant effect on CP, CF, IVDMD, IVOMD (P<0,05), but not significant effect on DM, OM, and crude fat (P>0.05). There was an interaction between type and dose level of foliar fertilizer on IVDMD and IVOMD (P<0.05). The conclusion is the type of foliar fertilizer doesn’t affect the nutrient content while the level of fertilizer dosage has a significant effect. There is a combination of foliar fertilizers and dose levels on IVDMD and IVOMD. The combination of Gandasil®D fertilizer with dose level of 4,5 g/l/plot got the best IVDMD and IVOMD.