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All Journal Indonesian Journal of Agricultural Science JURNAL BIOLOGI INDONESIA JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Biodjati ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Jurnal DISPROTEK Catha Saintifica Annales Bogorienses
Yuli Sulistyowati
Research Center for Biotechnology-LIPI Jalan Raya Bogor Km.46 Cibinong 16911, Phone (021)8754587 Fax. (021)8754588,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Other

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Genetic Transformation of Rice cv. Ciherang Using Double T-DNA Vector Harboring cry1Ab Gene Sulistyowati, Yuli; Rachmat, Agus; Zahra, Fatimah; Rachmawati, Syamsidah; Nugroho, Satya
Annales Bogorienses Vol. 15 No. 1 (2011): Annales Bogorienses
Publisher : BRIN

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Abstract

Rice stem borer (Scirpophaga sp.) is considered as one of the primary pests causing yield decrease in Indonesia. Genetic engineering is considered as one of the most effective way to improve the resistance of rice plants to yellow stem borer since no resistance gene has been found in rice and its wild relatives. A double T-DNA binary vector p2TDNAcryIAb wich carry two independent T-DNA was used to transform Indonesian elite rice cv Ciherang using Agrobacterium-mediated transformation to generate transgenic rice that free from selectable marker genes. One T-DNA containing a selectable marker hygromycin phosphotransferase (hpt) gene and the other carrying the cry1Ab gene. As many as 37 putative transgenic plants from 14 embryos were generated. PCR analysis demonstrated that 22 out of 37 putative transgenic rice contain cry1Ab gene. All Ciherang rice carrying the cry1Ab gene expressing Cry1Ab protein as shown by the immunostrip assay. Further work will be carried out to determine the transgenes copy number, to select marker free transgenic rice, and to evaluate the resistance of transgenic rice against neonates larvae of yellow stem borer.
Segregation Analysis of Transgenic Rice Plants cv Rojolele Harboring cry1B Gene and Plant Selection for Potential to Yellow Stem Borer Estiati, Amy; Sulistyowati, Yuli; Zahra, Fatimah; Nurhasanah, Ade Nena; Rachmawati, Syamsidah; Loedin, Inez Hortenza Slamet
Annales Bogorienses Vol. 16 No. 1 (2012): Annales Bogorienses
Publisher : BRIN

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Abstract

Transgenic rice plants harboring resistant gene to yellow stem borer, cry1B gene had been obtained. However, the cry1B gene in second generation of transgenic rice lines still segregating following Mendelian ratio 3:1. For further use in the breeding programs, it is important to ensure that the gene is dominant gene and the plants are homozygous for cry1B gene. Selection of homozygous transgenic rice lines containing cry1B gene at third, fourth and fifth generation had been conducted by PCR. The presence of cry1B gene was determined by showing the PCR product of 1.9 kb. Segregation analysis proved that six transgenic rice lines i.e. 3R7.8.15.1, 3R7.8.15.9, 3R7.8.15.10, 3R7.8.15.15, 3R7.8.15.21, 3R7.8.15.29 are homozygous lines for cry1B gene. Moreover, bioassay studies at vegetative stage on six homozygous transgenic rice lines showed that these transgenic rice lines are potential resistant to yellow stem borer comparing with non-transgenic plants, with the score of 0 (indicated no symptom) for six transgenic lines and score of 9 (more than 60% damaged tillers ) for non-transgenic plants. However, to confirm the efficacy of cry1B gene to yellow stem borer in natural condition, confined field trial in endemic area of yellow stem borer should be conducted. 
Polymorphic Identification of Simple Sequence Repeat (SSR) Marker for Developing Aluminum-Tolerance Upland Rice Anggraheni, Yuliana Galih Dyan; Mulyaningsih, Enung Sri; Priadi, Dody; Deswina, Puspita; Sulistyowati, Yuli; Adi, Eko Binnaryo Mei; Perdani, Ambar Yuswi; Nuro, Fiqolbi; Paradisa, Yashanti Berlinda
Jurnal Biodjati Vol 5 No 1 (2020): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i1.7990

Abstract

SSR marker is one of the genetic markers widely applied in plant breeding programs. The application of molecular markers in plant breeding is meant to accelerate the selection of cross-progeny. The research aimed to identify the SSR primers polymorphism between the parent and control that linked to Al tolerance and verify the cross-progeny of five crosses. The result gained from 37 SSR primers used in this study showed that only nine primers are polymorphic. These nine polymorphic primers are RM257, RM214, RM247, RM205, RM490, RM262, RM569, RM271, and RM19. The application of polymorphic markers on five cross-progeny which have shown the same band pattern as the parents and tolerant control on the use of 9 SSR primers recorded as follows: RM257 2 lines, RM214 5 lines, RM247 5 lines, RM205 lines, RM490 13 lines, RM262 5 lines, RM569 7 lines, RM271 4 lines, and RM19 6 lines. The selected SSR primers linked to Al tolerance in this research can be used as a reference for molecular breeding strategies to develop new Al tolerance rice varieties in dryland conditions.
Antioxidant enzyme activities in chili plants in response to the infection of Pepper yellow leaf curl Indonesia virus Paradisa, Yashanti Berlinda; Hidayat, Sri Hendrasturi; Mutaqin, Kikin Hamzah; Syukur , Muhamad; Wahyuni, Wahyuni; Indrayani, Sri; Sulistyowati, Yuli; Fidriyanto, Rusli
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 26 No. 1 (2026): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12678-89

Abstract

Chili pepper is an important agricultural crop but is highly vulnerable to viral diseases, including Pepper yellow leaf curl Indonesia virus (PepYLCIV). This study investigated the biochemical response of chili plants to PepYLCIV infection by examining changes in antioxidant enzyme activities: peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX). The effects of sample handling methods on enzyme activity measurements were also evaluated. The experiment was conducted in a biosafety greenhouse using a factorial design with four replications. The treatments included two chili varieties (Bara and Bonita), plant condition (healthy and PepYLCIV-infected), sample types (fresh leaf tissue and frozen leaf tissue stored at −80 °C), and seven sampling times (1, 3, 5, 7, 14, 21, and 28 days post-inoculation). Result showed that POD activity was significantly higher in infected plants than in healthy plants, whereas CAT and APX activities showed no significant differences between plant health conditions. Fresh samples consistently exhibited higher enzyme activity than frozen samples. POD and CAT activities peaked at 28 days post-inoculation, while APX activity was the highest at 5 days post-inoculation and fluctuated over time. These findings highlight the importance of considering infection status, sampling time, and sample processing when evaluating antioxidant enzymes in plant–virus interaction studies.
Co-Authors Adi, Eko Binnaryo Mei Agus Rachmat Albaz Ulumudin, Muhammad ALEX HARTANA Ambar Yuswi Perdani, Ambar Yuswi Anggraheni, Yuliana Galih Dyan Cahyani, Yani Didy Sopandie Dody Priadi Enung Sri Mulyaningsih Eryanto, Aji Estiati, Amy Estiati, Amy Faqih, Nasyiin Fatchur Roehman Fatimah Zahra Fidriyanto, Rusli Fiqolbi Nuro Pohan, Fiqolbi Nuro Fiqolbi Nuro, Fiqolbi Herlambang, Sigit Hery Widijanto Hidayat, Sri Hendrasturi Inez H. Slamet -Loedin Kikin H Mutaqin Loedin, Inez Hortenza Slamet Mushthofa Nurhasanah, Ade Nena Nurhasanah, Ade Nena Paradisa, Yashanti Berlinda Puspita Deswina, Puspita Rachmawati, Syamsidah Rizky Ramadhon, Ahmad S-Loedin, Inez H Satoto Satoto Satya Nugroho Sintho Wahyuning Ardie Solly Aryza Sri Indrayani, Sri Syamsidah Rahmawati, Syamsidah Syukur , Muhamad TRI KOESOEMANINGTYAS wahyuni wahyuni