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Utilization of Andong Leaf Extract (Cordyline fruticosa) as an Alternative for Coloring the Eggs of Intestinal Nematode Supriyanto; Linda Triana
Jurnal teknologi Kesehatan Borneo Vol 2 No 2 (2021): Jurnal Teknologi Kesehatan Borneo
Publisher : POLTEKKES KEMENKES PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jtkb.v2i2.52

Microscopic examination is essential to find the type of worm that causes infection. Direct examination of worm eggs needs to use staining to distinguish between worm eggs and food residues that are not completely digested. The dyes commonly used are synthetic. The purpose of this study was to replace synthetic dyes with natural dyes with Andong (Cordyline Fruticosa) leaf juice. It can be used as an alternative or a substitute for dyes. This research method is descriptive, conducted at the Parasitology Laboratory of Poltekkes Kemenkes Pontianak. The population in this study was the juice of the leaves of Andong (Cordyline Fruticosa). The method of inspection is direct preparation with an object glass as is routinely done, but the dye is replaced with Andong leaf juice (Cordyline Fruticosa). The results showed that natural dye Andong leaf juice (Cordyline Fruticosa), gave a clear color to the absorption of intestinal nematode worm eggs as the test target. This study concludes that the juice of the leaves of Andong (Cordyline Fruticosa) with alcohol solvent can be used as an alternative dye for the examination of intestinal nematode worm eggs.

AKTIVITAS ANTI BAKTERI FORMULASI LOTION EKSTRAK ETANOL RIMPANG JERINGAU PUTIH Emilda Sari; Linda Triana; Sugito Sugito; Edy Suwandi
Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): Meditory, Volume 11 No. 1 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i1.2292

Background: The skin is the most important and largest organ of the human body. The skin needs so much attention, both in terms of disease and health,because it turns out that the skin itself is quite susceptible to bacterial and fungal attacks. Therefore, the formulation of lotions for the skin, namely ingredients that can kill bacteria or fungi and are safe for the skin can be justified. Natural ingredients that have properties as antibacterial ingredients for the skin are White Jeringau. Aims:The purpose of this study was to explain the differences in the inhibition of the lotion formulation of the ethanol extract of the White Jeringau Rhizome against the growth of Staphylococcus aureus bacteria using the diffusion method. Methods:The research method used is a quasi-experimental with purposive sampling technique. The sample in this study was the lotion of the ethanol extract of the Jeringau Putih rhizome with concentrations of 40%, 50% and 60% which were replicated 9 times each so that the number of samples in this study were 27 samples. Furthermore, the sample was tested for inhibition of Staphylococcus aureus bacteria by diffusion method. Results:Based on the results of this study in 2022, antibacterial analysis of the white jeringau extract lotion sample formula I, formula II and formula III against Staphylococcus aureus bacteria using the Kirby-bauer diffusion method showed the results of the inhibition zone diameter, namely in formula I the average inhibition zone formed was 11 .50 mm, in formula II it is 13.61 mm and in formula III it is 14.50 mm. According to Davis and Stout (1971) the inhibitory response by active ingredients is grouped into 4categories, namely weak activity (? 5 mm), moderate (5-10 mm), strong (10-20 mm), and very strong (? 20 mm). Conclusion:Based on this classification, white jeringau extract lotion formula I, formula II and formula III are categorized as strong. Formula Ihad the best inhibitory response because the concentration of the extract was the smallest and had a strong category of inhibition compared to formula II and formula III which had a higher concentration and had the same inhibitory power. the results of statistical test analysis using the Kruskal Walis test obtained p value = 0.000 0.05 and it was concluded that there were differences in the white jeringau rhizome lotion formula I, II, and III in inhibiting the growth of Staphylococcus aureus bacteria.

Pengaruh Variasi Waktu Simpan terhadap Kadar Protein pada Ikan Tongkol Maulidiyah Salim; Linda Triana
Jurnal Laboratorium Khatulistiwa Vol 1, No 1 (2017): November 2017
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v1i1.87

Abstract: Fish is one of the sources of animal protein with the nutrients such as protein, lipid and other minerals. People generally save fish in the freezer so that it wonâ€™t decay. This research was aimed to analyze the effect of storage time variation with protein level in mackerel tuna. This research was an quasi-experiment research where the results had been analyzed by using regression test. Samples used in this research were fresh mackerel tuna based on visual criteria and their weight about Â± 1000 grams. The chosen samples with purposive sampling technic and 4 times replication so total samples were 24. The method using for protein testing was Kjeldahl Method. Results of the research obtained the average of protein level in fresh mackerel tuna amount as 24,52%, 2-day-froze mackerel tuna fish amount as 22,05%, 4-day-froze mackerel tuna 20,29%, 6-day-froze mackerel tuna 18,45% and 8-day froze mackerel tuna 16,03%. The result of regression test showed that there were no significant effect between 2 days storage time variation (p=0,290), 4 days (p=0,242), 6 days (p=0,485) and 8 days (p=0,059) with the protein level of mackerel tuna. Abstrak: Ikan merupakan salah satu sumber protein hewani dengan kandungan gizi seperti protein, lemak, dan mineral lainnya. Masyarakat umumnya menyimpan ikan dalam freezer agar tidak mengalami pembusukan. Penelitian ini bertujuan untuk menganalisis pengaruh variasi waktu simpan dengan kadar protein pada ikan tongkol. Penelitian ini merupakan penelitian eksperimental semu dimana hasil penelitian dianalisis dengan menggunakan uji regresi. Sampel yang digunakan adalah ikan tongkol yang segar secara visual dan memiliki berat Â±1000 gr. Sampel diperoleh dengan teknik purposive sampling dan dilakukan replikasi sebanyak 4 kali sehingga diperoleh 24 sampel. Metode yang digunakan untuk pemeriksaan protein adalah metode Kjeldahl. Hasil penelitian menunjukkan diperoleh rata-rata kadar protein pada ikan tongkol segar sebesar 24,52%, ikan tongkol beku hari ke-2 sebesar 22,05%, ikan tongkol beku hari ke-4 20,29%, ikan tongkol beku hari ke-6 18,45% dan ikan tongkol beku hari ke-8 16,03%. Hasi uji regresi menunjukkan tidak ada pengaruh yang nyata antara variasi waktu simpan 2 hari (p=0,290), 4 hari (p=0,242), 6 hari (p=0,485) dan 8 hari (p=0,059) terhadap kadar protein ikan tongkol.

Perbedaan Kadar Asam Sianida pada Rebung Sebelum dan Sesudah Difermentasi dengan Larutan Garam 2%, 3%, 4%, 5% Selama 7 Hari Ester Novelia; Ratih Indrawati; Linda Triana
Jurnal Laboratorium Khatulistiwa Vol 2, No 2 (2019): Mei 2019
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v2i2.330

Abstract: Bamboo shoot has a good nutritional value but bamboo shoot contains HCN (cyanide acid) which is a toxic compound in a form of taxiphylin glycoside in various gradients. The right bamboo shoots processing before it is been consumed is necessary, so that the bamboo shoot is safe to be consumed. One of the bamboo shoot processing that can decrease the cyanide acid level is fermentation using salt solution. The aim of this research is to fnd out about the difference on cyanide acid level on bamboo shoot before and after fermentation using salt solution 2%, 3%, 4%, and 5% for 7 days. The research design used is quasy experimental using purposive sampling with 25 samples.The determination method of cyanide acid level used was ion selective electrode. This method is based on the measurement of electrical voltage contained in destilat with ion exchange substance solid form in electrode surface made from the mixture of silver compounds inorganic conductive which is soluble in water. Based on the research the average cyanide acid level before fermentation was 96.27 mg/kg, after fermentation using salt solution 2% was 18,86 mg/kg, after fermentation using salt solution 3% was 10,71 mg/kg, after fermentation using salt solution 4% was 3,41 mg/kg and after fermentation using salt solution5% was 0,67 mg/kg. From the anava test performed, the result was p = 0,000 (p<0,005) which means there is signifcant difference of cyanide acid level on bamboo shoot before and after fermentation using salt solution 2%, 3%, 4%, and 5% for 7 days.Abstrak: Rebung memiliki nilai gizi yang cukup baik akan tetapi rebung bambu mengandung HCN (asam sianida) yang merupakan senyawa beracun dalam bentuk glikosida taxiphylin dengan tingkat yang beragam. Pengolahan rebung yang tepat sebelum dikonsumsi sangat diperlukan agar rebung aman untuk dikonsumsi. Salah satu cara pengolahan rebung yang dapat menurunkan kadar asam sianida adalah fermentasi menggunakan larutan garam.Tujuan dari penelitian ini adalah untuk mengetahui perbedaan kadar asam sianida pada rebung sebelum dan sesudah difermentasi dengan larutan garam 2%, 3%, 4%, 5% selama 7 hari. Desain penelitian yang digunakan adalah eksperimen semu menggunakan purposive sampling sebanyak 25 sampel. Metode penetapan kadar asam sianida menggunakan elektroda selektif ion. Metode ini didasarkan kepada pengukuran tegangan listrik yang dihasilkan akibat terjadinya proses pertukaran anion/kation antara ion sianida yang ada dalam destilat dengan zat penukar ion bentuk padat pada permukaan elektroda yang terbuat dari campuran senyawa-senyawa perak anorganik konduktif yang bersifat sukar larut dalam air.Berdasarkan hasil penelitian rata-rata kadar asam sianida sebelum difermentasi adalah 96.27 mg/kg, sesudah difermentasi dengan larutan garam 2% adalah 18,86 mg/kg, sesudah difermentasi dengan larutan garam 3% adalah 10,71 mg/kg, sesudah difermentasi dengan larutan garam 4% adalah 3,41 mg/kg dan sesudah difermentasi dengan larutan garam 5% adalah 0,67 mg/kg. Dari uji anava didapatkan hasil p = 0,000 ( p < 0,005) berarti terdapat perbedaan kadar asam sianida pada rebung sebelum dan sesudah difermentasi dengan larutan garam 2%, 3%, 4%, 5% selama 7 hari.

Analisis Kadar Asam Sianida Pada Ubi Kayu Yang Direndam Dalam Larutan NAHCO3 20% Dengan Variasi Waktu Linda Triana; Laila Kamila
Jurnal Laboratorium Khatulistiwa Vol 1, No 2 (2018): Mei 2018
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v1i2.150

Abstract: Cassava observed from nutrition side, it is highly contain of carbohydrate as calory source and the third major source of food after rice and corn. Containing useful compounds for body, however cassava also has cyanogenic glucose that is toxic or it is more familiar called blue toxic. To reduce cyanide level in cassava, it can be done by soaked into water that has been added NaHCO3 or baking soda. The purpose of this study was to identify the influence of immersion into 20% NaHCO3 solution with different time variation to the cyanide acid level in cassava. This sudy was a quasi experimental design. Cyanide acid level was determined by ion selective electrode with 28 samples taken by purposive sampling method. Simple regression linier anaylisis obtained significance value p=0,000 (p<0,05) thus Ha accepted, meaning that there was an influence of immersion into 20% NaHCO3 solution with different time variation to cyanide acid level in cassava. It is observed that immersion treatment into 20% NaHCO3 solution can decrease cyanide acid in cassava, the highest decline was in immersion for 12 hours. Cyanide acid before soakes was 61,8356 mg/kg and reduce to 9,76 mg/kg , or it was decrease about 84,22%.Abstrak: Ubi kayu ditinjau dari segi gizi kaya akan karbohidrat sebagai sumber kalori dan merupakan sumber bahan makanan ketiga di Indonesia setelah padi dan jagung. Selain mengandung senyawa yang berguna bagi tubuh ubi kayu juga mengandung senyawa glukosida sianogenik yang bersifat racun atau yang lebih dikenal dengan nama racun biru. Untuk menurunkan kadar sianida pada ubi kayu bisa dilakukan dengan cara perendaman didalam air dengan menambahkan NaHCO3 atau yang lebih dikenal dengan nama soda kue. Tujuan penelitian ini adalah untuk mengetahui pengaruh perendaman dalam larutan NaHCO3 20% dengan variasi waktu terhadap kadar asam sianida pada ubi kayu. Metode penelitian yang digunakan dalam penelitian ini adalah eksperimental semu. Penetapan kadar asam sianida menggunakan elektroda selektif ion dengan jumlah sampel sebanyak 28 buah yang ditentukan secara purposive sampling. Hasil uji statistic regresi linier sedehana diperoleh nilai signifikan p = 0,000 (p <0,05) sehinggaÂ Ha diterima artinya ada pengaruh perendaman dalam larutan NaHCO3 20%Â dengan variasi waktu terhadap kadar asam sianida pada ubi kayu. Perlakuan perendaman dengan larutan NaHCO3 20%Â dapat menurunkan kadar asam sianida pada ubi kayu, penurunan terbanyak pada perendaman selama 12 jam. Asam sianida sebelum direndam sebesar 61,8356 mg/kg turun menjadi 9,76 mg/kgÂ terjadi penurunan sebesar 84,22%.

Perbedaan Kadar Glukosa Darah 2 Jam Post Prandial Linda Triana; Maulidiyah Salim
Jurnal Laboratorium Khatulistiwa Vol 1, No 1 (2017): November 2017
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v1i1.97

Abstract: One of the simple carbohydrates is glucose that acts as the main energy producer. The function of the body will be felicitous when blood glucose levels are within normal limits. Glucose removal levels are considered normal if glucose levels return to normal within 2 hours after it rises in the first hour. If the blood glucose level within 2 hours after given fed is abnormal, it can be done by Oral Glucose Tolerance Test to get additional information about the presence of carbohydrate metabolism disorders. This study was aimed to determine the difference of blood glucose levels within 2 hours postprandial between samples who given fed with loads of 75 grams glucose. The type of research used in this research was analytic observational with comparative study approach. Samples obtained 33 samples with treatment 4 times in each sample. The method used in this research was an enzymatic method. The results of this study showed the average blood glucose level within 2 hours postprandial which given fed with loads was 10.10% while the average measurement of blood glucose level within 2 hours postprandial loaded with 75 grams glucose was 7.61%. T-test obtained t value of 1.092 with a significant level at p = 0.284 (p> 0.05) so the conclusion there was no difference of blood glucose level within 2 hours postprandial between who given fed with loads of 75-gram glucose.Abstrak: Salah satu karbohidrat sederhana adalah glukosa yang berperan sebagai penghasil energi utama. Fungsi dari tubuh akan menjadi sangat baik apabila kadar glukosa darah berada pada batas yang normal. Kadar pembuangan glukosa dianggap normal jika kadar glukosa kembali normal dalam waktu 2 jam setelah kenaikan pada 1 jam pertama. Apabila kadar glukosa darah dalam waktu 2 jam setelah makan abnormal, maka dapat dilakukan Tes Toleransi Glukosa Oral untuk mendapatkan keterangan tambahan tentang adanya gangguan metabolisme karbohidrat. Penelitian ini bertujuan untuk mengetahui perbedaan kadar glukosa darah 2 jam post prandial antara yang diberi beban makanan dengan beban glukosa 75 gram. Jenis penelitian yang digunakan dalam penelitian ini adalah observasional analitik dengan pendekatan comparative study. Sampel didapatkan 33 sampel dengan perlakuan 4 kali pada setiap sampelnya. Metode yang digunakan dalam penelitian ini adalah metode enzimatik. Hasil penelitian ini menunjukan rata-rata kadar glukosa darah 2 jam post prandial yang diberi beban makanan yaitu 10,10% sedangkan hasil pengukuran rata-rata kadar glukosa darah 2 jam post prandial yang diberi beban glukosa 75 gram yaitu 7,61%. Uji T-test didapatkan nilai t hitung sebesar 1,092 dengan tingkat signifkan pada p = 0,284 (p>0,05) sehingga Ha ditolak dengan kesimpulan tidak ada perbedaan kadar glukosa darah 2 jam post prandial antara yang diberi beban makanan dengan beban glukosa 75 gram.

Perbedaan Kadar Kalsium Dalam Darah Sebelum Dan Saat Menstruasi Pada Mahasiswi Dengan Sindrom Pramenstruasi Jurusan Analis Kesehatan Pontianak Slamet Slamet; Linda Triana
Jurnal Laboratorium Khatulistiwa Vol 1, No 2 (2018): Mei 2018
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v1i2.151

Abstract: The most common cause of premenstrual syndrome is the imbalance of estrogen and progesterone in the premenstrual phase. These hormonal imbalances can trigger changes in calcium concentration in the blood. This study aims to determine the difference in blood calcium levels before menstruation and during menstruation in female students with premenstrual syndrome in the Department of Health Analyst Polytechnic Health Pontianak. The design in this study is cross sectional. The sampling technique used in this research is purposive sampling. The sample used in this study is a student D-IV level I Department of Health Analyst Pontianak as many as 31 people. Measurement and inspection of calcium levels is done by using O-CPC colorimetric method. Based on the research results obtained calcium levels before menstruation with the average value of 9.01942 mg/dl, the lowest score 8.251 mg/dl and the highest value 9.977 mg/dl. Calcium levels during menstruation with an average value of 9.74487 mg/dl, the lowest value of 9.055 mg/dl and the highest value 10.294 mg/dl. The result of data processing using paired t-test (Paired Sample T-Test) by computerized using SPSS 20 program obtained p value = 0.000 smaller than significant level (Î±) (0,000 <0,05), it can be concluded that Ha accepted meaning that there are differences in calcium levels in the blood before menstruation and at menstruation in female students with premenstrual syndrome.Abstrak: Penyebab munculnya sindrom pramenstruasi yang paling sering dipertimbangkan adalah ketidakseimbangan hormon estrogen dan progesteron pada fase pramenstruasi. Ketidakseimbangan hormon tersebut dapat memicu perubahan pada konsentrasi kalsium dalam darah. Penelitian ini bertujuan untuk mengetahui perbedaan kadar kalsium dalam darah sebelum menstruasi dan saat menstruasi pada mahasiswi dengan sindrom pramenstruasi di Jurusan Analis Kesehatan Politeknik Kesehatan Pontianak. Desain dalam penelitian ini adalah cross sectional. Teknik pengambilan sampel yang digunakan dalam penelitian ini adalah Purposive Sampling. Sampel yang digunakan dalam penelitian ini adalah mahasiswi D-IV tingkat I Jurusan Analis Kesehatan Pontianak sebanyak 31 orang. Pengukuran dan pemeriksaan kadar kalsium dilakukan dengan menggunakan metode O-CPC colorimetric. Berdasarkan hasil penelitian diperoleh kadar kalsium sebelum menstruasi dengan nilai rata -rata 9.01942 mg/dl, nilai terendah 8.251 mg/dl dan nilai tertinggi 9.977 mg/dl.Kadar kalsium saat menstruasi dengan nilai rata-rata 9.74487 mg/dl, nilai terendah 9.055 mg/dl dan nilai tertinggi 10.294 mg/dl. Hasil pengolahan data menggunakan uji t berpasangan (Paired Sample T-Test)secara komputerisasi dengan menggunakan program SPSS 20 di peroleh nilai p=0,000 lebih kecil dari tingkat signifikan (Î±) (0,000 <0,05 ), maka dapat disimpulkan bahwa Ha diterima yang artinya terdapat perbedaan kadar kalsium dalam darah sebelum menstruasi dan saat menstruasi pada mahasiswi dengan sindrom pramenstruasi.

Potensi Toksisitas Akut Ekstrak Etanol Daun Pepaya terhadap Larva Artemia Salina Leach dengan Metode Brine Shrimp Lethality Test Novia Wahyu Lestari; Maulidiyah Salim; Linda Triana
Jurnal Laboratorium Khatulistiwa Vol 2, No 1 (2018): November 2018
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v2i1.320

Abstract: Papaya tree is one of the medicinal plant types which advantages for traditional medicine. The ï¬‚avonoid, alkaloid and saponin were the compounds of papaya leaves. There potentially as cytotoxic for certain levels. The purpose of this research is to determine the potency cytotoxic ethanol extract papaya leaves against Artemia salina Leach larvae using Brine Shrimp Lethality Test (BSLT) method. Research method by quasi experimental, and purposive sampling used 360 Artemia salina Leach larvae divided to 5 groups treatment. Each group contains 15 larvaes. Each group is done by the replication of research for 4 times. The extract concentration are 1000, 750, 500, 250, 100 and 0 Âµg/ml as a negative control. Data have been obtained by calculating amount of died larvae 24 hours after treatment. Mean percentage larvae death in the concentration of 1000, 750, 500, 250, 100 and 0 Âµg/ml consecutively were 95%, 78,33%, 53,33%, 36,67%, 21,67% and 0%. Through the data, LC50 value was analyzed by probit analysis using Microsoft offce excel. The higher of extract concentration cause higher percentage death of the larvae. This result shows that LC50 value of ethanol extract of papaya leaves is 285,693 Âµg/ml (less than 1000 Âµg/ml). The conclusion is the ethanol extract of papaya leaves had acute toxicity potency against Artemia salina Leach larva according to Brine Shrimp Lethality Test (BSLT). Abstrak: Tanaman pepaya merupakan satu diantara jenis tanaman obat yang umum dimanfaatkan oleh masyarakat secara empiris untuk pengobatan secara tradisional. Tanaman ini mengandung senyawa ï¬‚avonoid, alkaloid dan saponin. Senyawa tersebut diduga berpotensi sebagai sitotoksik pada kadar tertentu. Tujuan dari penelitian ini adalah untuk mengetahui potensi sitotoksik ekstrak etanol daun pepaya (Carica papaya L.) terhadap larva Artemia salina Leach dengan metode Brine Shrimp Lethality Test (BSLT). Metode penelitian ini berbentuk eksperimental semu dengan menggunakan 360 ekor larva Artemia salina Leach yang dibagi dalam 5 kelompok perlakuan. Tiap kelompok terdiri dari 15 ekor. Tiap kelompok dilakukan pengulangan percobaan 4 kali. Konsentrasi ekstrak berturut-turut adalah 1000, 750, 500, 250, 100 dan 0 Âµg/ml sebagai kontrol negatifnya. Data diperoleh dari menghitung jumlah larva yang mati 24 jam setelah perlakuan. Rata-rata persentase kematian larva pada konsentrasi 1000, 750, 500, 250, 100 dan 0 Âµg/ml berturut-turut adalah 95%, 78,33%, 53,33%, 36,67%, 21,67% dan 0%. Berdasarkan data, LC50 ekstrak etanol daun pepaya ditentukan dengan analisis probit menggunakan Microsoft offce excel. Semakin tinggi konsentrasi ekstrak menyebabkan semakin tinggi persentase kematian larva. Hasil penelitian ini menunjukkan harga LC50 dari ekstrak etanol daun pepaya adalah 285,693 Âµg/ml (kurang dari 1000 Âµg/ml). Hal ini menunjukkan bahwa ekstrak etanol daun pepaya memiliki potensi toksisitas akut terhadap larva Artemia salina Leach dengan metode Brine Shrimp Lethality Test (BSLT).

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