Article Per Year (5 Year)
p-Index From 2021 - 2026
0.817
P-Index
This Author published in this journals
All Journal e-Journal Pustaka Kesehatan Dentika Dental Journal Journal of Mathematical and Fundamental Sciences STOMATOGNATIC- Jurnal Kedokteran Gigi UNEJ e-Proceeding Dental Journal (Majalah Kedokteran Gigi) BIOEDUKASI Padjadjaran Journal of Dental Researchers and Students
I Dewa Ayu Susilawati
Department Of Biomedical Science, Faculty Of Dentistry, Universitas Jember
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Physics Public Health

Published : 17 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search
Journal : e-Journal Pustaka Kesehatan

 1 
(Kadar LDL dan HDL Dalam Darah Model Tikus Periodontitis (Blood Level of LDL and HDL in Periodontitis Rat Model ) Nugraha Agung Marda; Suci Wulan Agustin; Susilawati Ayu Dewa
Pustaka Kesehatan Vol 2 No 1 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Backgroud. Increased levels of low density lipoprotein (LDL) and decreased levels of high density lipoprotein (HDL) in the blood is a risk factor for atherosclerosis leading to Coronary Heart Disease (CHD). Risk factor of atherosclerosis are multifactorial such as consuming high lipid diet, excessive eating, sedentary, and inflammatory response. According to the recent study indicated that inflammatory response could affect LDL and HDL blood levels that related to the risk of atherosclerosis lesion formation. Therefore, we suspected that periodontitis could affect LDL and HDL. Purpose. The purpose of this study was to show the effect of periodontitis in LDL and HDL. Methods. This experimental study used the post test only control group design. The object were 8 male wistar rats (Rattus norvegicus) with inclusion criterias were male rats, ±200 gr body weight, 3-4 months age, having a healthy condition, good appetite, and normal behavior. The rats were devided into two groups, i. e. control group and treatment group (periodontitis group) with 4 rats in each group. Periodontitis rat model was created by fitting cervical left low molar tooth with wire ligature and injected with 0,02 ml P. gingivalis ATCC 33277 (dosage 5µg/0,05 ml) in bucal sulcus, three times a week for 4 weeks (28 days), all of the rats were fed with standard diet. On day 29th all groups of rats were sacrificed and intracardial blood were taken 3 cc for examination of LDL and HDL blood levels by electrospectrophotometric method. Data were analyzed by T-test. Result. Result showed that periodontitis rat model had a significantly higher LDL levels compared to control group (P <0,05) and HDL levels were lower than controls, but the difference was not significant (P >0,05). Conclusion and suggestion. Based on this study periodontitis increase LDL blood levels. Further research related with duration of periodontitis are needed because periodontitis is usually a chronic disease and have a long lasting effect . In addition, the role of the severity of periodontitis are required to learn.   Keywords: HDL, LDL, Periodontitis.
Deteksi Lesi Aterosklerosis Koroner pada Model Tikus Periodontitis (Detection of Coronary Atherosclerotic Lesions in Periodontitis Rat Model) Roza Nafilah; Rendra Chriestedy Prasetya; I Dewa Ayu Susilawati
Pustaka Kesehatan Vol 3 No 2 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Recent concept istated that periodontitis can induce the formation of coronary atherosclerotic lesions but there is less experimental study about that. Purpose: Analyze the effect of periodontitis on the formation of coronary atherosclerotic lesions.The parameters are coronary artery wall thickness, collagen intimal disintegration, atheroma, stenosis, endothelial disintegration, lipid deposition and fatty emboly. Methods: This experimental laboratoris (in vivo) study used male wistar rats with a total sample of 12 animals, were divided into 2 groups: control and periodontitis treatment that were prepared by mounting wire ligature and Porphyromonas gingivalis injection (0,5 McFarland/1,5x108 CFU/ml) in the gingival sulcus left mandibular first molar teeth 3 times a week for 4 weeks. On 5th week, mice were secrificed and heart that contain the coronary arteries was taken. Heart were fixed , histological preparations were made and colored by Picrosirius Red and Oil Red O. Results: The Arterial walls of periodontitis group are significantly (p <0.05) thicker than the control group. the presence of collagen intimal disintegration, atheroma, stenosis, endothelial disintegration, lipid deposition and fatty emboly are also significantly (p<0,05) more in periodontitis group than in the control group. Conclusion: Periodontitis increase the risk of coronary atherosclerotic. Keywords: Atherosclerosis Lesion, Periodontitis
Induksi Streptococcus mutans terhadap Aktivitas Proteinase Netrofil pada Degradasi Kolagen Tipe IV  (Induction of Streptococcus mutans on Neutrophil Proteinase Activity in Degradation of Type IV Collagen)   Purwanto Purwanto; I Dewa Ayu Susilawati
Pustaka Kesehatan Vol 2 No 1 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background. Endothelial basal membrane of vasculature is composed mainly by type IV collagen. This vascular collagen degradation is the most powerful stimulus for platelet aggregation and thrombosis. This phenomenon is the basic mechanism of the pathogenesis of atherothrombotic diseases. Streptococcus mutans, the main bacterium of dental caries can invade easily into blood circulation, and therefore they have chance to involve in the pathogenesis of vascular destruction and thrombosis. It was assumed that S. mutans caused vascular destruction due to its potency to induce neutrophil proteinase activity to degrade type IV collagen. Objective. This study purposed to proof in vitro that S. mutans could induce and activate neutrophil proteinases leading to degradation tipe IV collagen. Methods.  Collagen degradation was analysed by means of Sodium Dodecyl Sulphate Polyacrylamid Gel Electrophoresis (SDS-PAGE) and Soluble Biotinylated Assay (SBA). Results. Results showed that S. mutans induced production and activation of neutrophil proteinases leading to type IV collagen degradation into six fragments with the molecular weight were 129; 95; 50; 32; 28; 14 kDa. Conclusion.  S. mutans induced neutrophil proteinase activity leading to degradation of type IV collagen. This study might explain one of mechanisms role of S. mutans in initiation of atherothrombotic diseases.   Key words: Streptococcus mutans; Type IV collagen; Neutrophil
Co-Authors Agustin Wulan Suci Dharmayanti Depi Praharani Dyah Setyorini Erawati Wulandari Fawwazi, Rafif Hanif Fiftiani Syarah Happy Harmono I Dewa Ayu Ratna Dewanti Ishimora, Marina Erlysa Masari, Al Melok Aris Wahyukundari Morauli, Fatricia Nadie Fatimatuzzahro Nailufar, Khanun Nugraha Agung Marda P. Purwanto Pujiana Endah Pujiana Endah Lestari Purwanto , Purwanto Purwanto Rendra Chriestedy Prasetya Ristya Widi Endah Lestari Ristya Widi Endah Yani Roedy Budirahardjo Roza Nafilah Sunlip Wibisono