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All Journal e-Journal Pustaka Kesehatan Dentika Dental Journal Journal of Mathematical and Fundamental Sciences STOMATOGNATIC- Jurnal Kedokteran Gigi UNEJ e-Proceeding Dental Journal (Majalah Kedokteran Gigi) BIOEDUKASI Padjadjaran Journal of Dental Researchers and Students
I Dewa Ayu Susilawati
Department Of Biomedical Science, Faculty Of Dentistry, Universitas Jember
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Physics Public Health

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Periodontal infection is a “silent killer”* Susilawati, I Dewa Ayu
STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 8, No 1 (2011)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Abstract

Periodontal infection contained at many pro-oxidatif toxity components which is easily spreading through flood circulation syslimicly, and potentially acts as antigent and induces inflamating response inflamation processes is a front line defence respons to infection. However it is very dangerous if inflamation is oceure is a vital asam such as in brain and heart, could be of a life threatering. The spreading of Periodontal infection through Blodd steam is usually asymoptpmatically. Thas patients are unaware that their left is in a thred Periodontal infection is a “silent killer”.
INDUKSI METABOLIT PORPHYROMONAS GINGIVALIS PADA PRODUKSI RADIKAL SUPEROKSID NETROFIL: INDUCTION OF PORPHYROMONAS GINGIVALIS METABOLITES ON NEUTROPHIL SUPEROXIDE RADICAL PRODUCTION I Dewa Ayu Susilawati
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.16 KB) | DOI: 10.32734/dentika.v16i1.1887

Abstract

Radical suproxide is a kind of reactive oxygen species (ROS) which causes oxidative damage by attacking biomolecules such as protein, lipid, DNA, etc. Over production of radical superoxide becomes a centre of the generation of other radical species and oxidants, and responsible for occurring many pathological processes. The main source of radical superoxide isto activate neutrophil. Therefore, it was speculated that induction of neutrophil by periodontitis bacteria Porphyromonas gingival is potential to induce superoxide production, only a few study, however concerned with this topic. This studyaimed to know the pathogenicity of P. gingivalis by demonstrating the potency of P. gingivalis metabolites to induce production of radical superoxide by neutrophil. This study was conducted experimentally in vitro using posttest onlydesign. Superoxide radical production was demostrated by means of Nitrobluetetrazolium (NBT) slide assay and spectro photometric. The results showed that P. gingivalis metabolites induced neutrophil to produce a large amount of radical superoxide both intra and extra cellular. Its radical superoxide production affected degranulation, apoptosis, and lysis of neutrophil. In conclusion, neutrophil responses against P. gingivalis generated prooxidative and toxic condition. This mechanism may explain the pathogenicity of P. gingivalis on many pathological processes.
(Kadar LDL dan HDL Dalam Darah Model Tikus Periodontitis (Blood Level of LDL and HDL in Periodontitis Rat Model ) Nugraha Agung Marda; Suci Wulan Agustin; Susilawati Ayu Dewa
Pustaka Kesehatan Vol 2 No 1 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Backgroud. Increased levels of low density lipoprotein (LDL) and decreased levels of high density lipoprotein (HDL) in the blood is a risk factor for atherosclerosis leading to Coronary Heart Disease (CHD). Risk factor of atherosclerosis are multifactorial such as consuming high lipid diet, excessive eating, sedentary, and inflammatory response. According to the recent study indicated that inflammatory response could affect LDL and HDL blood levels that related to the risk of atherosclerosis lesion formation. Therefore, we suspected that periodontitis could affect LDL and HDL. Purpose. The purpose of this study was to show the effect of periodontitis in LDL and HDL. Methods. This experimental study used the post test only control group design. The object were 8 male wistar rats (Rattus norvegicus) with inclusion criterias were male rats, ±200 gr body weight, 3-4 months age, having a healthy condition, good appetite, and normal behavior. The rats were devided into two groups, i. e. control group and treatment group (periodontitis group) with 4 rats in each group. Periodontitis rat model was created by fitting cervical left low molar tooth with wire ligature and injected with 0,02 ml P. gingivalis ATCC 33277 (dosage 5µg/0,05 ml) in bucal sulcus, three times a week for 4 weeks (28 days), all of the rats were fed with standard diet. On day 29th all groups of rats were sacrificed and intracardial blood were taken 3 cc for examination of LDL and HDL blood levels by electrospectrophotometric method. Data were analyzed by T-test. Result. Result showed that periodontitis rat model had a significantly higher LDL levels compared to control group (P <0,05) and HDL levels were lower than controls, but the difference was not significant (P >0,05). Conclusion and suggestion. Based on this study periodontitis increase LDL blood levels. Further research related with duration of periodontitis are needed because periodontitis is usually a chronic disease and have a long lasting effect . In addition, the role of the severity of periodontitis are required to learn.   Keywords: HDL, LDL, Periodontitis.
Deteksi Lesi Aterosklerosis Koroner pada Model Tikus Periodontitis (Detection of Coronary Atherosclerotic Lesions in Periodontitis Rat Model) Roza Nafilah; Rendra Chriestedy Prasetya; I Dewa Ayu Susilawati
Pustaka Kesehatan Vol 3 No 2 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Background: Recent concept istated that periodontitis can induce the formation of coronary atherosclerotic lesions but there is less experimental study about that. Purpose: Analyze the effect of periodontitis on the formation of coronary atherosclerotic lesions.The parameters are coronary artery wall thickness, collagen intimal disintegration, atheroma, stenosis, endothelial disintegration, lipid deposition and fatty emboly. Methods: This experimental laboratoris (in vivo) study used male wistar rats with a total sample of 12 animals, were divided into 2 groups: control and periodontitis treatment that were prepared by mounting wire ligature and Porphyromonas gingivalis injection (0,5 McFarland/1,5x108 CFU/ml) in the gingival sulcus left mandibular first molar teeth 3 times a week for 4 weeks. On 5th week, mice were secrificed and heart that contain the coronary arteries was taken. Heart were fixed , histological preparations were made and colored by Picrosirius Red and Oil Red O. Results: The Arterial walls of periodontitis group are significantly (p <0.05) thicker than the control group. the presence of collagen intimal disintegration, atheroma, stenosis, endothelial disintegration, lipid deposition and fatty emboly are also significantly (p<0,05) more in periodontitis group than in the control group. Conclusion: Periodontitis increase the risk of coronary atherosclerotic. Keywords: Atherosclerosis Lesion, Periodontitis
Induksi Streptococcus mutans terhadap Aktivitas Proteinase Netrofil pada Degradasi Kolagen Tipe IV  (Induction of Streptococcus mutans on Neutrophil Proteinase Activity in Degradation of Type IV Collagen)   Purwanto Purwanto; I Dewa Ayu Susilawati
Pustaka Kesehatan Vol 2 No 1 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Background. Endothelial basal membrane of vasculature is composed mainly by type IV collagen. This vascular collagen degradation is the most powerful stimulus for platelet aggregation and thrombosis. This phenomenon is the basic mechanism of the pathogenesis of atherothrombotic diseases. Streptococcus mutans, the main bacterium of dental caries can invade easily into blood circulation, and therefore they have chance to involve in the pathogenesis of vascular destruction and thrombosis. It was assumed that S. mutans caused vascular destruction due to its potency to induce neutrophil proteinase activity to degrade type IV collagen. Objective. This study purposed to proof in vitro that S. mutans could induce and activate neutrophil proteinases leading to degradation tipe IV collagen. Methods.  Collagen degradation was analysed by means of Sodium Dodecyl Sulphate Polyacrylamid Gel Electrophoresis (SDS-PAGE) and Soluble Biotinylated Assay (SBA). Results. Results showed that S. mutans induced production and activation of neutrophil proteinases leading to type IV collagen degradation into six fragments with the molecular weight were 129; 95; 50; 32; 28; 14 kDa. Conclusion.  S. mutans induced neutrophil proteinase activity leading to degradation of type IV collagen. This study might explain one of mechanisms role of S. mutans in initiation of atherothrombotic diseases.   Key words: Streptococcus mutans; Type IV collagen; Neutrophil
ROBUSTA COFFEE BEANS DECREASE OF INFLAMMATION IN DENTAL CARIES I Dewa Ayu Ratna Dewanti; I Dewa Ayu Susilawati; Pujiana Endah; Roedy Budirahardjo
UNEJ e-Proceeding Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UPT Penerbitan Universitas Jember

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The coffee plant is one commodity that is developedin kabuapaten Jember. Coffee chemical constituents,such as flavonoids, xanthine, antioxidants, alkaloids,polyphenols may serve as anti-inflammatory,antibacterial, platelet aggregation. Polyphenolbioavailability of coffee has also been studied.1.2.3While Namboodiripad, K. Srividya (2009) provedtheir coffee inhibition zone against S. mutans. Theimmune response of dental caries among othersrelated to cytokines are expressed odontoblast layer,such as IL-1β, IL-1α and TNF-α.4.5 Thus it is said thatcoffee is thought to inhibit dental caries by means ofmodulating the immune response. This niaccordance with the Decree of the Minister of Statefor Research and Technology of the Republic ofIndonesia Number: 02 / M / Kp / II / 2000 onDevelopment and Engineering for NationalDevelopment (JAKSTRA 2000-2004) in the field ofdentistry which has two areas of research focusinclude health field for prevention and treatment ofdiseases and new materials related to ceramics,polymer composites, engineering materials(materials science and engineering), as well as newmaterial derived from ingredients that are natural.6One of the natural ingredients that are known toeveryone is coffee. Where coffee is one of theexcellent programs that can improve theperformance of Jember University's Strategic Plan.This type of coffee is widely known in Indonesiathere are three kinds, namely Arabica, Robusta andLiberika. In general, farmers grow robusta, becausein this type of coffee has more superior properties,so it is very fast growing. Even the coffee is thedominant species of coffee plantations in Indonesia.In addition, it contains prolifenol Robusta coffee,caffeine and chlorogenic acid, which is higher thanthe other coffee types. Currently, the production ofrobusta coffee production reached third copiesworldwide.7.8 Coffee brews are very widelyconsumed in the world due to their pleasant flavorand taste as well as for their stimulating effect onthe mental and physical activities. Several years ago,in California, a research conducted by TakayukiShibamoto, a professor of environmental toxicology,found that freshly brewed coffee contains potentantioxidants equal to the amount found in threeoranges. Antioxidants in general have been linked toa number of potential health benefits, includingprotection against heart disease and cancer,protection against liver and colon cancer, type 2diabetes, and Parkinson's disease..9 Coffee chemicalconstituents, such as flavonoids, xanthine,antioxidants, alkaloids, polyphenols may serve asanti-inflammatory, antibacterial, plateletaggregation.1.2.3 Roasted coffee also has antiadhesiveproperties which prevent adhesion of S.mutans and other harmful materials on the teethand whiten teeth.4 Previous research by a team ofother researchers of Excellence coffee beans for thetreatment of cancer of the oral cavity, an article onPotential immunomodulatory coffee beans to dentalcaries and the potential immunomodulatory Robustabeans to dental caries.10.11.12 In this study Robustabeans made in the form of pasta because pastaShape is the most popular because it can be easilyused and harden quickly.Capping pupa is one of the more conservativetreatments to prevent dental caries has been on thepulp or pulp exposure due to mechanical factors.Interest Capping pulp to maintain the vitality ofdental pulp. Pulp tissue is important in the formationof secondary dentine and reparative dentin inresponse to biological and pathological stimuli.Capping pulp is the application layer or theprotective material or materials for treatment overan open pulp, such as calcium hydroxide which willstimulate the formation of reparative dentin.13Capping pulp after the treatment, the healingprocess will occur in the pulp tissue covering theinflammatory response, collagen formation and theformation of new dentin. Inflammatory responseshould occur as a sign of the host defense. Acuteinflammation occurs a few minutes, hours or days,while chronic inflammation occurs after acuteinflammation. Inflammatory played by inflammatorycells such as PMN (polymorphonuclear,mononuclear cells (monocytes, macrophages,lymphocytes, mast cells, plasma cells). PMN are cellsleading that respond to injury and most frequentlyProceeding ICMHS 2016 ISBN 978-602-60569-3-1174seen in inflammation. These cells react in the earlystages of inflammation (acute) up to 28 hours,followed by macrophages, lymphocytes and plasmacells (chronic).14 Chronic inflammation is said to bethe beginning of the healing process. Although theresponse innate was important in inflammation, butan adaptive response also plays a role, becausecytokines were producted by T cells induceinflammation. To obtain optimal care, the necessaryunderstanding in depth about how the composition,antibacterial properties, biocompatibility, effect ondentin and pulp as well as the technique of using aresin adhesive as a material Capping pulp directly. Inhandling damage teeth, either by decay or trauma,maintain vital pulp tissue remains the mostimportant things to be done by a dentist because ofthe non-vital teeth, the teeth tend to be susceptibleto fracture. The development of the field ofconservation science teeth adapted to thedevelopment of basic science related andtechnological advances applied which is thedevelopment of science in the field of clinical(clinical science). Dental conservation therapy aimsto maintain its position of teeth as long as possible inorder to work longer. This objective can be achievedby treating the hard tissue or soft tissue of a tooth totooth structure back to normal, or at least close tonormal.15
Analysis of protein profile of neem leaves juice (azadirachta indica l. Juss) I Dewa Ayu Ratna Dewanti; I Dewa Ayu Susilawati; Pujiana Endah Lestari; Roedy Budirahardjo
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Abstract

The current trend of the management of diseases has shifted toward modulating the immune response of the patients. This is achieved by the administration of immunomodulatory substance obtained from the immunogenic medicinal plant, such as neem. The immunogenic properties of neem has been associated with its protein content. Aim. This study aimed to characterize the proteins and immunogenic components of neem leaves juice using sodium dodecyl Sulphate method Polyacrilamide gel electrophoresis (SDS-PAGE). Materials and Methods. Neem leaf juice was extracted for proteins with freeze dry method. Samples that were to be run were added with RSB with the ratio of 1:1, then heated in boiled water for ± 5 minutes. An amount of 15 µl from each sample was then put into the wells. Samples were run in 120 Volt for 60-80 minutes. Gel was then taken, stained with silver nitrate for 1 hour, and was then stained every 1.5 hours. Protein bands formed were then observed. Results. The protein fraction of neem leaves juice consisted of proteins with molecular weights (MW) as follows: 11 kDa, 13 kDa, 30 kDa, 62 kDa, 70 kDa, 81 kDa. Conclusion. All protein fractions of neem leaves juice are potentially immunogenic components.
ROBUSTA COFFEE BEANS INCREASE LEVELS OF TNF-α AS A RESPONSE TO Streptococcus mutans Pujiana Endah Lestari; I Dewa Ayu Susilawati; I Dewa Ayu Ratna Dewanti; Roedy Budirahardjo
UNEJ e-Proceeding Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Coffee plant is commodity exports that wasencouraging because it has relatively high economicvalue in the world market. Coffee plantations aremanaged by people, and this day its grow continuallyin several provinces in Indonesia so expansion isincreasing continuously. Coffee production inIndonesia ranks the fourth largest in the world afterColombia, Brazil and Vietnam (Zainuddin andMurtisari, 1995; Simanihuruk and Sirait, 2010). Inaddition, the coffee plant is one of the leadingcommodities developed in Jember. Chemical contentof coffee such as flavonoids, xanthine, antioxidants,alkaloids, polyphenols can serve as a bitter taste,antibacterial, platelet aggregation. Thebioavailability of polyphenols coffee has also beenstudied (Scalbert et al., 2000; Coralie et al., 2006;Naziq, 2012).Namboodiripad and Srividya (2009) prove theexistence of the inhibition zone coffee against S.mutans. These bacteria are structurally andantigenetically express a surface protein that arecalled antigen I / II, B, Sr and PAC that have amolecular weight of 185 kDa. These antigens by theresearchers were assigned that play a role in thepathogenesis of dental caries, and its are effective asa vaccine in the prevention of dental caries. I/IIantigens of S. mutans have properties of adhesive,that the bacteria are used attachment to hostcomponents during colonize and infection, so itsbecome the focus of a number of researchers. Thesesurface-antigen proteins have an effect in theattachment of S. mutans with acquired pellicles ontooth surfaces (Yuliati, 2005). The immune responsesof dental caries were associated with cytokine suchas IL-1 β, α, IL-1 and TNF-α that were expressedodontoblast layer. It is said that bacteria excretion aextracellular virulence-immunomodulatory protein(VIP, which has a mitogenic effect on lymphocytes,suppress the immune response of the host andinduces production of IL-10, which is animmunosuppressor cytokines. So, VIP is animportant virulence factor produced microorganismsand closely associated with the pathogenicity ofbacteria (Gomes et al., 2006).Thereby, it is said that coffee could be expectedto inhibit dental caries by means of modulate theimmune response. The paradigm change stated thatthe tissue replacement turns into tissueregeneration that was cause approach of medicalmateria that geared to a biologically tissueimprovement. Biocompatible materials indicate thatit can be accepted by the body (Harty and Ogston(1995), and the coffee was clearly meets theserequirements.The current utilization of the coffee plant in themodulates an immune response against S. mutansthat is cause a dental caries that have not beenstudied, so arises problem how theimmunomodulatory potential of robusta coffeebeans against dental caries ?The general objective of this research is to analyzethe immunomodulatory potential of robusta coffeebeans against dental caries, whereas the aim inparticular is analyze the influence of robusta coffeebeans against TNF-α levels in response to S. mutans.
The role of Kuniran (U. moluccensis) and Gurami (O. goramy) fish thorns and scales in increasing salivary leukocyte and monocyte cells viability against Streptococcus mutans I Dewa Ayu Ratna Dewanti; I Dewa Ayu Susilawati; P. Purwanto; Pujiana Endah Lestari; Roedy Budirahardjo; Dyah Setyorini; Ristya Widi Endah Yani; Erawati Wulandari; Melok Aris Wahyukundari
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 1 (2019): March 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1168.806 KB) | DOI: 10.20473/j.djmkg.v52.i1.p45-50

Abstract

Background: Kuniran thorns and Gurami fish scales are rich in protein and minerals such as dentin believed to increase cell viability against Streptococcus mutans (S. mutans) that causes dental caries. These, in turn, can cause systemic diseases if left untreated. Purpose: This study aims to analyze the influence of Kuniran thorns and Gurami fishes scales on the viability of monocytes and salivary leukocytes against S. mutans. Methods: Monocytes and leukocytes salivary cells were placed on a microtiter plate and treated according to the nature of each group. This study comprised the following groups: control group: untreated; S. mutans group: induced by S. Mutans; Gurami thorn group: thorns + S. Mutans; Gurami scales group: scales + S. Mutans; Kuniran thorn group: thorns + S. Mutans; Kuniran scales group: scales + S. mutans. Viability analysis involved staining with Tripan Blue. Furthermore, the number of viable cells (white) was calculated under an inverted microscope at 200 times magnification from five fields of view. Data was analyzed by means of an ANOVA test followed by LSD test. Results: The ANOVA and LSD tests confirmed significant differences (0.01<P). Kuniran thorns and Gurami fish scales increased the viability of monocytes and salivary leukocytes, but not significantly. The content of flavonoids, amino acids, omega 3, omega 6 and antioxidants increased leukocyte metabolism, thereby increasing cell resistance to S. mutans infection. Conclusion: Kuniran thorns (U. moluccensis) and Gurami (O. goramy) fish scales increase the viability of salivary leukocyte and monocyte cells against Streptococcus mutans.
Robusta Coffee (Coffeacanephora) Decreasing IL-1α (Interleukin-1α) Expression and Increasing the Number of Fibroblasts in Healing Process in Dental Pulp in Wistar Rats I Dewa Ayu Ratna Dewanti; I Dewa Ayu Susilawati; Pujiana Endah Lestari; Ristya Widi Endah Lestari; Erawati Wulandari; Roedy Budirahardjo; Dyah Setyorini; Sunlip Wibisono
Journal of Mathematical and Fundamental Sciences Vol. 51 No. 1 (2019)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2019.51.1.6

Abstract

Fibroblasts and IL-1α are important components of inflammation and healing in dental caries. This healing allegedly could be enhanced by robusta coffee. The aim of this study was to investigate the effect of varying concentrations of robusta coffee on IL-1α expression and the number of fibroblasts. Wistar rats were used, divided into 4 groups and 3 subgroups (7-, 14"‘, and 21-day rats) with each group containing 4 animals: Control group: untreated group; 25% Coffee Group: cavities + treated with 25% coffee bean paste; 50% Coffee Group: cavities + treated with 50% coffee bean paste; 75% Coffee Group: cavities + treated with 75% coffee bean paste. The 7-, 14-, and 21-day rats were sacrificed serially for analysis of the number of dental pulp fibroblasts by staining with HE and IL-1α expression by immunohistochemistry. The resulting data were analyzed descriptively and using ANOVA, followed by an LSD test. The higher the concentration of robusta coffee, the more the expression of IL-1α decreased and the more the number of fibroblasts increased. It is suspected that immunomodulatory proteins inhibit IL-1α expression. It was concluded that robusta coffee could decrease IL-1α expression and increase the number of fibroblasts during the healing process in the dental pulp of Wistar rats.
Co-Authors Agustin Wulan Suci Dharmayanti Depi Praharani Dyah Setyorini Erawati Wulandari Fawwazi, Rafif Hanif Fiftiani Syarah Happy Harmono I Dewa Ayu Ratna Dewanti Ishimora, Marina Erlysa Masari, Al Melok Aris Wahyukundari Morauli, Fatricia Nadie Fatimatuzzahro Nailufar, Khanun Nugraha Agung Marda P. Purwanto Pujiana Endah Pujiana Endah Lestari Purwanto , Purwanto Purwanto Rendra Chriestedy Prasetya Ristya Widi Endah Lestari Ristya Widi Endah Yani Roedy Budirahardjo Roza Nafilah Sunlip Wibisono