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Determinasi Salmonella sp dan Ektoparasit dalam Pupuk Organik dari Kotoran Sapi Potong di Yogyakarta Suwito, Widodo; -, Supriadi; Winarti, Erna; Bimo Bekti, Utomo
Biota Biota Volume 13 Nomor 2 Tahun 2014
Publisher : PBI Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.19 KB)

AbstractOne of by products derived from beef cattle farm is manure. The manure can be processed to make organic fertilizer. Salmonella sp is bacterium of the manure origin and harmful for human. Currently, organic fertilizer is mostly used for vegetables and fruits, but the problem is people consume those vegetables. The aim of this study was to determine Salmonella sp and ectoparasite in organic fertilizer which were made from the manure of beef cattle farm in Yogyakarta. A total of 10 manure samples were collected the farms to make organic fertilizer. The manure was fermented with lactic acid bacteria (BAL) for one month. Before and after fermented, the manure was isolated and identified for Salmonella sp based on biochemical reactions and ectoparasite using native method. The study showed that there were no Salmonella sp and ectoparasite in the organic fertilizer made from the manure fermented for one month.Keywords: Organic fertilizer, manure, farm, Salmonella spAbstrakSalah satu hasil sampingan dari peternakan sapi potong adalah kotoran ternak. Kotoran ternak dapat dimanfaatkan untuk dibuat pupuk organik. Salmonella sp merupakan bakteri yang berasal dari kotoran ternak dan dapat membahayakan kesehatan manusia. Saat ini pupuk organik banyak digunakan untuk tanaman sayuran dan buah-buahan, sedangkan sebagian masyarakat mengonsumsi sayuran dalam keadaan mentah. Tujuan dari penelitian ini adalah untuk mengetahui Salmonella sp dan ektoparasit dalam pupuk organik yang dibuat dari kotoran sapi pada peternakan sapi potong di Yogyakarta. Telah dikumpulkan sebanyak 10 sampel kotoran sapi yang akan dibuat pupuk organik dari peternakan sapi potong di Yogyakarta. Kotoran sapi difermentasi dengan bakteri asam laktat (BAL) selama satu bulan. Kotoran sapi sebelum dan sesudah difermentasi dilakukan isolasi dan identifikasi Salmonella sp berdasarkan reaksi biokimia dan ektoparasit dengan metode natif. Penelitian ini menunjukkan bahwa di dalam kotoran sapi yang dibuat pupuk organik dengan fermentasi selama satu bulan tidak ditemukan Salmonella sp.Kata kunci: Pupuk organik, rabuk, peternakan sapi, Salmonella sp

Impacts of Verotoxigenic and Enterohaemorrhagic Escherichia Coli (Vtec and Ehec) on Animal, Human and Food Suwito, Widodo
Indonesian Bulletin of Animal and Veterinary Sciences Vol 19, No 2 (2009)
Publisher : Indonesian Animal Sciences Society

Verotoxigenic and enterohaemorrhagic Escherichia coli, VTEC and EHEC are strains that produce a toxin on the Vero cells in vitro, therefore the toxin called verotoxin. Strain VTEC and EHEC have been isolated from human and various animal species, mainly ruminants and pigs. The prevalence of VTEC in cattle is 35%, milk 10%, cheese 1.5%. In beef meat, pork, poultry, goat and sheep is 3.7, 1.5, 1.5, 2 and 2.5%, respectively. In contrast, the human prevalence in United States, Spain and France 5 â 10, 2.5 and 3.4%, respectively. In Indonesia, nine cases of VTEC were reported by Ciptomangunkusumo Hospital and four out of nine cases were died. The verotoxin also named Shiga toxins (Stx), is active in vivo as a pathogen on the endothelial cells of the blood vessels of the gastro intestinal mucosa, kidneys, brain and other tissues of human and piglets. Verotoxin in human, ruminants and piglets causes bloody diarrhea so with ruminants. In the adult ruminants, verotoxin results in clinical signs and they act as asymptomatic carriers of VTEC and EHEC strains but in human, it produced haemorrhagic colitis (HC), haemolytic uremic syndrome (HUS) and thrombocytopenia purpura (TPP). Infection in human by the most famous EHEC strain belongs to the O157:H7 serotype, through faecal contamination or environment of either food of animal origin, or other foodstuffs (fruits and vegetables). Infection from EHEC strains could be prevented by avoiding food of animal origin and unpasteurized milk, and by taking care of food hygiene for comsumption. Â Key words: verotoxin, enterohaemorrhagic, VTEC, EHEC, Shiga toxin (stx), ruminant

Mastitis in Ettawa Crossbred Goat (Pe) Caused by Staphylococcus Aureus: Epidemiology, Clinical Signs, Pathogenesis, Diagnosis and Control Suwito, Widodo; S, Indarjulianto
Indonesian Bulletin of Animal and Veterinary Sciences Vol 23, No 1 (2013)
Publisher : Indonesian Animal Sciences Society

Mastitis in Ettawa crossbred goat is commonly found and caused economic loss. Staphylococcus aureus is one of bacteria caused clinical mastitis or subclinical mastitis in Ettawa crossbred goat. The aim of this review was to provide an overview of clinical and subclinical mastitis in the Ettawa crossbred goat caused by S. aureus from epidemiological aspect, clinical symptoms, pathogenesis, diagnosis, treatment, prevention and control. Mastitis should be eliminated because it lead to death for the goats and lambs. In addition, S. aureus has greater risk for contamination in milk because it produces heat-stable toxin. Isolation and identification bacteria with total of somatic cell counts are important as a reference to determine the actions to decrease the occurrence of mastitis. Some preventive measures for mastitis include clean milking, dipping the teats with a disinfectant and antibiotic treatment during dry lactation. Key words: Ettawa crossbred goat, mastitis, S. aureus, diagnosis, preventif

Verotoxigenic Escherichia coli (VTEC) isolated from cow milk Suwito, Widodo
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 3 (2009)
Publisher : Indonesian Animal Sciences Society

Verotoxigenic Escherichia coli (VTEC) strains are responsible for serious human illnesses. These strains are commonly found in milk. The aim of this study was to determine the occurrence of verotoxigenic E. coli in milk. A total of 351 milk samples, were collected from dairy farms in Bogor, Sukabumi and Cianjur. These samples were analyzed for VTEC using biochemical, serological and vero cell cytotoxicity assays. VTEC O157:H7 isolates were found in milk collected from dairy herds in Bogor and Sukabumi at rates 0.47% of 214 samples, 1.10% of 91 samples respectively, and none in Cianjur. Hemolytic E. coli isolates were found in 0.94% of 214 milk samples from Bogor, 2.2% of 91 milk samples from Sukabumi and none from Cianjur. From E. coli isolates, 53 isolates (67.95%) were verotoxigenic, consisted of: two E. coli O157:H7 isolates and 51 non O157:H7 isolates.Therefore this study showed the occurrence of VTEC in milk samples from dairy farms in Bogor, Sukabumi and Cianjur. Key words: Milk, E. coli (VTEC) O157:H7, Verotoxigenic

Determinasi Salmonella sp dan Ektoparasit dalam Pupuk Organik dari Kotoran Sapi Potong di Yogyakarta Suwito, Widodo; -, Supriadi; Winarti, Erna; Bimo Bekti, Utomo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 18, No 2 (2013): June 2013
Publisher : Universitas Atma Jaya Yogyakarta

AbstractOne of by products derived from beef cattle farm is manure. The manure can be processed to make organic fertilizer. Salmonella sp is bacterium of the manure origin and harmful for human. Currently, organic fertilizer is mostly used for vegetables and fruits, but the problem is people consume those vegetables. The aim of this study was to determine Salmonella sp and ectoparasite in organic fertilizer which were made from the manure of beef cattle farm in Yogyakarta. A total of 10 manure samples were collected the farms to make organic fertilizer. The manure was fermented with lactic acid bacteria (BAL) for one month. Before and after fermented, the manure was isolated and identified for Salmonella sp based on biochemical reactions and ectoparasite using native method. The study showed that there were no Salmonella sp and ectoparasite in the organic fertilizer made from the manure fermented for one month.Keywords: Organic fertilizer, manure, farm, Salmonella spAbstrakSalah satu hasil sampingan dari peternakan sapi potong adalah kotoran ternak. Kotoran ternak dapat dimanfaatkan untuk dibuat pupuk organik. Salmonella sp merupakan bakteri yang berasal dari kotoran ternak dan dapat membahayakan kesehatan manusia. Saat ini pupuk organik banyak digunakan untuk tanaman sayuran dan buah-buahan, sedangkan sebagian masyarakat mengonsumsi sayuran dalam keadaan mentah. Tujuan dari penelitian ini adalah untuk mengetahui Salmonella sp dan ektoparasit dalam pupuk organik yang dibuat dari kotoran sapi pada peternakan sapi potong di Yogyakarta. Telah dikumpulkan sebanyak 10 sampel kotoran sapi yang akan dibuat pupuk organik dari peternakan sapi potong di Yogyakarta. Kotoran sapi difermentasi dengan bakteri asam laktat (BAL) selama satu bulan. Kotoran sapi sebelum dan sesudah difermentasi dilakukan isolasi dan identifikasi Salmonella sp berdasarkan reaksi biokimia dan ektoparasit dengan metode natif. Penelitian ini menunjukkan bahwa di dalam kotoran sapi yang dibuat pupuk organik dengan fermentasi selama satu bulan tidak ditemukan Salmonella sp.Kata kunci: Pupuk organik, rabuk, peternakan sapi, Salmonella sp

Isolation, Characterization, and Antimicrobial Susceptibility of Escherichiacoli from Breeder Superior Native Chicken (KUB)in Yogyakarta, Indonesia Suwito, Widodo; Andriani; Primatika, Roza Azizah; Tangkonda, Elisabet; Primatika, Roza
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 1 (2025): April 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.01.8

Colibacillosis remains a prevalent issue in breeder superior native chickens (KUB). This study aims to isolate, characterize, and assess the antimicrobial susceptibility of E. coli from breeder KUB chickens in Yogyakarta, Indonesia. Samples, including infertile eggs (n=113), day-old chick (DOC) deaths (n=53), drinking water (n=17), feed (n=25), eggshells (n=117), and fluff (n=113), were collected through random sampling from breeder KUB chickens. Isolation and identification of E. coli were carried out via biochemical methods, whereas serological tests were performed with antisera for O1:K1, O2:K1, and O78:K80. Hemolytic E. coli strains were identified through culture on blood agar, and antimicrobial susceptibility was evaluated via the disk diffusion method. A total of 31 E. coli isolates were obtained from various sources: infertile eggs (15.04%), DOC-contaminated eggs (13.20%), drinking water (35.29%), feed (8.00%), eggshells (8.00%), and fluff (20.25%). Among the isolates, 22.62% were hemolytic, and 77.38% were nonhemolytic. The serotype distributions were as follows: 11.9% O1:K1, 9.52% O2:K1, and 9.52% O78:K80. E. coli isolates were susceptible to tetracycline, oxytetracycline, enrofloxacin, and sulfamethoxazole but resistant to ampicillin, streptomycin, and erythromycin. E. coli, the causative agent of colibacillosis, has been isolated from KUB breeder chickens in Yogyakarta. Therefore, strengthening biosecurity measures and implementing effective antibiotic management strategies are crucial for mitigating the risk of antibiotic resistance.

ANALISIS MIKROBIOLOGI SUSU KAMBING PERANAKAN ETTAWA (PE) DARI KABUPATEN SLEMAN YOGYAKARTA Suwito, Widodo; Sri Nugroho, Widagdo; Wahyuni, AETH Wahyuni; Sumiarto, Bambang
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2625

Tujuan penelitian ini mengetahui kualitas mikrobiologi susu kambing mentah yang diambil langsung dari ambing. Dalam penelitian ini digunakan 50 sampel susu kambing peranakan Ettawa (PE). Sampel dianalisis terhadap total plate count (TPC), jumlah Staphylococcus sp., jumlah koliform, Escherichia coli (E. coli), dan Salmonella sp. berdasarkan reaksi biokimia. Rerata untuk TPC; Staphylococcus sp.; total koliform masing-masing adalah 1,65x10 3 ; 5,75x10 3 ; 1,3x10 cfu/ml, sedangkan E. coli dan Salmonella sp. adalah negatif. Berdasarkan Standar Nasional Indonesia (SNI) No 01-6366-2000 tentang persyaratan susu segar, maka TPC, koliform, E. coli, dan Salmonella sp. memenuhi standar, sedangkan Staphylococcus sp. melebihi ambang batas. Hasil penelitian menunjukkan bahwa susu kambing mentah yang diambil langsung dari ambing masih layak konsumsi.

Aktivitas Madu sebagai Antibakteri Staphylococcus aureusdan Escherichia ColiO157:H7 Suwito, Widodo; Andriani, Andriani; Amelia, Iis; Rohmayanti, Titi; Haris, Helmi; Karimy, Moh Faiz
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.90498

Honey is a natural substance from flower nectar and produced by honey bees. The honey has been used as healing. The study was conducted to determine the antibacterial activity of Blora and Kaliandra honey against pathogenic bacteria such as Staphylococcus aureus (S. aureus) and Escherichia coli O157:H7 (E. coli O157:H7). As a comparison, was used commercial honey. The results showed Blora and Kaliandra honey had antibacterial activity, however the commercial honey has not antibacterial activity. Kaliandra honey has antibacterial activity against S. aureus and E. coli O157:H7 better than Blora and commercial honey. Concentration 25% of Kaliandra honey was able to inhibit S. aureus, while blora honey showed antibacterial activity at 50% and commercial honey did not show antibacterial activity until concentration 50%. Inhibition zone of S. aureus produced by Kaliandra honey with concentration 25 and 50% were 8.0 ± 0.1 mm and 9.0 ± 0.2 mm respectively and concentration 50% Blora honey was 7.3 ± 0.1 mm. Inhibition zone of E. coli O157:H7 produced by concentration 50% Kaliandra honey was 8.0 ± 0.3 mm and Blora honey was 7.7 ± 0.1 mm. The study showed that Blora and Kaliandra honey have antibacterial activity and can be used as alternative substitution in antibiotic therapy.

ISOLATION, IDENTIFICATION, AND ANTIBIOTIC SENSITIVITY AGAINST Salmonella sp. FROM HATCHERY KUB CHICKEN IN YOGYAKARTA [Isolasi, Identifikasi, dan Sensitivitas Antibiotik Terhadap Salmonella sp. dari Penetasan Ayam KUB di Yogyakarta] Suwito, Widodo; Setya Adji, Rahmat; Faiz Karimy, Moh.
Berita Biologi Vol 22 No 3 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.454

Salmonella sp. is responsible bacteria that causes salmonellosis in hatchery-produced local chicken (KUB) in Yogyakarta. The objective of this study was to determine of character Salmonella sp. from hatchery KUB chicken in Yogyakarta. This study was carried out in KUB chicken hatcheries in Yogyakarta province, specifically in the Sleman district (n = 5), Bantul (n = 3), Kulonprogo (n = 3), and Gunungkidul (n=2). Isolation and identification of Salmonella sp. were done from eggshells (n=17) and chick fluff of KUB chicken (n = 13) based on conventional methods with the bacteriological analytical manual (BAM). Sensitivity of Salmonella sp. against antibiotics is determined by antimicrobial diffusion agar with a paper disc antibiotic. A total of 3 isolates of Salmonella sp. were isolated from eggshells (n=2) and chick fluff from KUB chicken (n=1). Salmonella sp. was sensitive to tetracycline, oxytetracycline, enrofloxacin, sulfamethoxazole and resistant to ampicillin and erythromycin. The study showed that the hatchery of KUB chicken in Yogyakarta was contaminated with Salmonella sp. and had resistance to several antibiotics. Therefore, desinfection in incubator hatcheries using KUB chicken should continue after the egg hatches.

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