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All Journal Hemera Zoa Jurnal Sain Veteriner Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Partner Jurnal Pengabdian Masyarakat Peternakan International Journal of Community Service Learning JURNAL KAJIAN VETERINER Jurnal Pengabdian kepada Masyarakat Nusantara Madani : Jurnal Pengabdian Kepada Masyarakat Jurnal Lembaga Pengabdian Kepada Masyarakat Undana Jurnal Media Tropika: Jurnal Pengabdian Kepada Masyarakat Jurnal Veteriner Nusantara Jurnal Veteriner dan Biomedis
Tangkonda, Elisabet
Laboratorium Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan Universitas Nusa Cendana
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JSPS-10 The Effect of Consumption of Raw Chicken Meat on Humoral Immunity against Campylobacter jejuni in veterinarians and workers in a chicken processing plant Elisabet Tangkonda; Satoshi Sekiguchi; Meiko Kubo; Satomi Sasaki; Takako Taniguchi; Naoaki Misawai
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (559.853 KB)

Abstract

Campylobacter jejuni and C. coli are the leading cause of enteric infections in Japan and many other developed countries, and the public health burden of campylobacteriosis is increasing [1]. Although the epidemiological data in Japan is based on passive surveillance, approximately 2,000 to 3,000 cases per each year have been reported as a foodborne infection since 1982. Many risk factors for Campylobacter transmission have been identified. Handling and consumption of poultry meat are often causing of infection [2, 3]. Since Japanese have a food habitant to eat fresh raw "free-range" chicken meat and liver, the risk for infection with campylobacters may be high [4]. However, little is known about the relationship between consumption of raw chicken meat and humoral immunity against C. jejuni in humans. When people had been exposed to campylobacters contaminated in water or foods, it has been reported that their antibodies were rising [5]. This study was conducted by analyzing the antibody level against C. jejuni with questionnaires from 74 veterinarians who worked as a meat inspector and 181 workers from a chicken processing plant.
JSPS-9 Microbiologial Quality of Chicken Carcasses in Bogor Indonesia Based on Campylobater sp. and Salmonella sp. Counts Herwin Pisestyani; Elisabet Tangkonda; Maya Shofa; Surachmi Setyaninigsih; Denny Widaya Lukman; Bambang Pontjo Priosoeryanto; Naoaki Misawa
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Unhygienic handling chicken carcasses during slaughtering until selling to costumers can lead to contamination by pathogenic bacteria such as Campylobacter sp., and Salmonella sp. entering human’s body can cause foodborne diseaseThe aims of this study were to detect contamination and enumuration of Campylobacter sp, and Salmonella sp. in chicken carcasses from poultry slauhterhouses and markets in Bogor Indonesia.
Isolasi, Identifikasi, dan Serotyping Avibacterium paragallinarum dari Ayam Petelur Komersial yang Menunjukkan Gejala Snot Elisabet Tangkonda; Charles Rangga Tabbu; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (604.293 KB) | DOI: 10.22146/jsv.48489

Abstract

Infectious coryza (snot) is one of acute respiratory disease in breeders, layers, and broilers caused by Avibacterium paragallinarum (Av. paragallinarum). This disease is very harmful because of its cause decrease in egg production and high morbidity. Snot incident in Indonesia still is reported. Vaccination is one of the best preventive measures, but reports about Av. paragallinarum serotype at the field so lack so that the correspondence between serotype Av. paragallinarum in the field to those used for vaccination is unknown. Av. paragallinarum has strains with different antigenicity and until now known three serotypes there are serotypes A, B and C. Serotypes A and C are pathogenic then serotypes B. The purpose of this study was to isolate, identify and to determine the serotype of Av. paragallinarum from the commercial layer that showing symptoms of snot. Samples were taken from layer which showed symptoms of snot (nasal foul smelling exudate, infra-orbital and wattle swelling, conjunctivitis, and snoring) from some layer farms. Samples were cultured on chocolate agar and then incubated in a candle jar at 37 ᴼC for 18-24 hours. Bacteria colony and cell morphology were observed and performed biochemical tests (catalase, oxidase, urease, indole, and fermentation of carbohydrates) in suspected colonies. Serotypes test was conducted using plate agglutination test (PAT). This study revealed 4 isolates Av. paragallinarum with 2 isolates are serotype B and 2 other are serotype C.
Aplikasi Teknologi Amoniasi Limbah Jerami Padi Sebagai Pakan Ternak Berkualitas Pasca Siklon Tropis Seroja Cynthia Dewi Gaina; Filphin Adolfin Amalo; Inggrid Trinidad Maha; Frans Umbu Datta; Yeremia Yobellano Sitompul; Dede Rival Novian; Elisabet Tangkonda
Jurnal Pengabdian Masyarakat Peternakan Vol 7, No 1 (2022): Jurnal Pengabdian Masyarakat Peternakan
Publisher : Jurusan Peternakan Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (390.822 KB) | DOI: 10.35726/jpmp.v7i1.730

Abstract

Kegiatan pengabdian kepada masyarakat (PkM) yang dilakukan di Dusun Nautasik, Desa Suelain, Kecamatan Lobalain, Kabupaten Rote Ndao, Provinsi NTT ini bertujuan untuk meningkatkan ilmu pengetahuan dan keterampilan praktis bagi petani tentang teknologi amoniasi limbah pertanian jerami padi. Oleh masyarakat setempat, limbah jerami yang dapat diubah menjadi pakan berkualitas, khususnya dimusim kemarau tidak dimanfaatkan secara optimal. Limbah yang ada hanya dibiarkan saja disekitar areal persawahan, dibakar atau diberikan pada ternak sapi tanpa melalui pengolahan teknologi pakan, terlebih dengan kejadian siklon tropis seroja menyebabkan limbah pertanian yang ada rusak dan tidak dapat diberikan pada ternak. Oleh karena itu, kegiatan ini bertujuan untuk mengenalkan teknologi pakan amoniasi yang berguna untuk meningkatkan kandungan nutrisi dan tingkat kecernaan dengan diubahnya lignin, selulosa, hemiselulosa dan silika yang terdapat pada jerami padi kering. Tahapan pelaksanaan kegiatan PKM meliputi pembekalan, demonstrasi dan pendampingan tentang aplikasi teknologi amoniasi. Hasil kegiatan ini ditunjukkan melalui peningkatan pengetahuan peternak tentang pentingnya memanfaatkan teknologi amoniase limbah pertanian bagi ternak. 
Uji Kepekaan Avibacterium paragallinarum Terhadap Antibiotik yang Berbeda Elisabet Tangkonda; Charles Rangga Tabu; Agnesia Endang Tri Hastuti Wahyuni
JURNAL KAJIAN VETERINER Vol 2 No 1 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i1.987

Abstract

Avibacterium paragallinarum is the aetiology of Infectious coryza (snot), one of acute upper respiratory tract diseases, which causes financial loss due to the decrease of egg production in layer. This is Gram negative bacteria which have three serotypes, known as serotype A, B, and C. The aim of this research was to know the sensitivity of Avibacterium paragallinarum to different kinds of antibiotics that are commonly used. This research used Avibacterium paragallinarum culture derived from layer which showed the symptoms of snot. Sensitivity test applied erythromycin, enrofloxacin, gentamycin, amoxicillin and clavulanic acid, and sulfamethoxazol. Results found the sensitivity of Avibacterium paragallinarum to combination of amoxicillin and clavulanic acid was 100%, to erythromycin 50% resistant and 50% intermediate, to enrofloxacin 75% resistant and 25% intermediate, to gentamycin 75% sensitive and 25% resistant, and to sulfamethoxazol 100% resistant.
Isolasi dan Identifikasi Eschericia coli O157:H7 dari Babi, Sapi dan Ayam yang Menunjukkan Gejala Diare Elisabet Tangkonda
JURNAL KAJIAN VETERINER Vol 4 No 2 (2016): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v4i2.1017

Abstract

Escherichia coli is a bacterium found in the intestines of humans and animals. Most strains of Escherichia coli are harmless. However, some strains, such as Enterohaemorrhagic Escherichia coli (EHEC), can cause a disease transmitted to humans through contaminated food. Enterohaemorrhagic Escherichia coli produce toxins, known as verotoxin thus also known as Verocytotoxin-producing E. coli (VTEC). The aim of this study was to isolate and identify Escherichia coli O157:H7 from pigs, cattle and chickens. Samples were taken from pigs, cattle and chickens with diarrhea and identified by microbiological diagnostic. The result reveal, 18 of the 18 pig samples, 17 of 30 samples of cattle and 34 of 46 samples of chicken identified as E. coli O157:H7 with prevalence 100%, 56.6% and 73.9% respectively.
Pengaruh Pemberian Konsentrasi Gula Lontar pada Dendeng Ikan Tembang (Sardinella fimbriata) terhadap Lama Simpan berdasarkan Kadar Air, Nilai Organoleptik dan Total Cemaran Mikroba Satria K. Frans; Annytha Ina Rohi Detha; Elisabet Tangkonda
JURNAL KAJIAN VETERINER Vol 4 No 2 (2016): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v4i2.1018

Abstract

Dendeng is a traditional food results by the public Rote Ndao. Method for making dendeng by drying using sunlight as well as using palm sugar as the main ingredient pickling as well as the addition of spices. This study aims to determine the effect of the use of palm sugar in the manufacture of Dendeng Ikan. The processing is done in the District Lobalain(Rote Ndao). Methods of use of the sugar palm consisting of 4 treatments, KO (without giving sugar palm), K1 (10% concentration of palm sugar), K2 (20% concentration of palm sugar), K3 (30% concentration of palm sugar), K4 (40% concentration palm sugar). 4 times the storage of dendeng with the observation that the 5th day (P1), 10th day (P2), 15th day (P3), 20th day (P4). The quality of dendeng observed included presentations lost water content after processing, testing organoleptic and microbiological quality in terms of Total Plate Count (TPC). The test results of water content during storage of dendeng showed that, giving palm sugar concentration of 40% in the processing of fish dendeng, has a water content lower than the palm sugar concentration of 10%, 20% and 30%. During storage, less than the 15th day dendeng with palm sugar concentration giving 40% meet the Standar Nasional Indonesia moisture content, while the dendeng with the provision of palm sugar concentration of 30% has less storage than day-to-5 that meets the water content of the Standar Nasional Indonesia. The test results showed that the organoleptic, Giving palm sugar concentration levels in the processing of fish dendeng affect the appearance, consistency / texture, aroma and taste of dendeng. During storage in certain storage time of each treatment dendeng change the appearance, consistency / texture, aroma and taste. The test results TPC (Total Plate Count) of dendeng showed that, giving the concentration of sugar palm sugar palm 40% in the processing of dendeng, has a value of TPC (Total Plate Count) is lower than the concentration of sugar palm 10%, 20%, and 30 %. During storage, only on day 5 dendeng with palm sugar concentration giving 40% to abide by the TPC (Total Plate Count) of Standar Nasional Indonesia.
TINGKAT CEMARAN Staphylococcus aureus PADA IKAN ASIN DI PASAR TRADISIONAL KOTA KUPANG Marlin Cindy Claudya Malelak; Diana A. Wuri; Elisabet Tangkonda
JURNAL KAJIAN VETERINER Vol 3 No 2 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i2.1039

Abstract

Fish has become one of the favorite foodstuffs for Indonesian society. Like the other food commodities of animal origin, fish also has a perishable nature so it takes a good handling and processing, such as salting and drying which the result is known as salted fish. The processing of salted fish requires attention because there is a high risk of food poisoning hazard for food which the growth of normal flora are inhibited due to the enterotoxin of Staphylococcus aureus. This study aims to determine the contamination level of Staphylococcus aureus within the salted fish in the traditional markets in the city of Kupang. There were 18 samples of salted fishes used which consisted of 8 samples from Oesapa traditional market, 5 samples from Oeba traditional market and 5 samples from theInpres Naikoten traditional market. The sample inspection using the Plate Count method in order to spread. The results showed that the contamination level of all the salted fish samples have passed the maximum contamination limit of S. aureus based on the SNI No. 7388 : 2009 (1 x 103 CFU/g) with the value of the contamination level ranged from 3.4 x 103 CFU/g in the salted fish derived from Oeba traditional market to 1.4 x 106 CFU/g in the salted fish derived from the Inpres Naikoten market. The high value of the contamination level is related to several factors such as environment, storage time, sanitation and personal hygiene. Therefore, salted fish traded in traditional markets require attention to be safe and suitable for consumption.
UJI SENSITIVITAS ANTIBIOTIKA TERHADAP Staphylococcus aureus YANG DIISOLASI DARI LUKA KULIT ANJING DI DESA MERBAUN, KECAMATAN AMARASI BARAT KABUPATEN KUPANG Eky Melyani Sanu; Maxs Urias E Sanam; Elisabet Tangkonda
JURNAL KAJIAN VETERINER Vol 3 No 2 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i2.1041

Abstract

Staphylococcus aureus is one causes of wound infection in dogs, occured as secondary infection or trauma. One of the obstacles in the treatment of Staphycoccal infection is their resistance to antibiotics. This study aims to isolate and identify the Staphylococcus aureus in the cases of dog wound as well as testing the bacterial resistance to the antibiotics namely amoxicillin, tetracycline and ampicillin. There are total of 18 samples of dog wound taken by sterile swab and put in a closed test tube containing sterile physiological NaCl and stored in a ice jar. Swab is applied to the surface on Mannitol Salt Agar and observed morphology of the bacterial colonies that grow after incubated for 24 hours at 37 0C. Furthermore, the Gram staining, hemolysis test, biochemical tests including the catalase and coagulation were also tested. Suspect Staphylococcus aureus colonies were further tested for antibiotics sensitivity test using the Kirby Bauer’s method. Results of this experiment showed 8 isolates (44.44%) were positive to Staphylococcus aureus. The isolation of Staphylococcus aureus in the culture of Blood Agar showed that 8 isolates are Beta hemolytic strain. Antibiotics sensitivity test done in duplo revealed that there are 7 isolates of Staphylococcus aureus sensitive and 1 isolate resistant to ampicillin. The sensitivity test to the antibiotic tetracycline showed that 6 isolates sensitive and 2 isolates resistant. The sensitivity test to the antibiotic amoxicillin showed that 7 isolates sensitive and 1 isolates resistant. It can be suggested that in the treatment of wound infection in the dog's skin, antibiotic sensitivity test should be done, because some strains of Staphylococcus aureus has developed resistance to antibiotics.
Isolasi dan Identifikasi Agen Etiologi Gejala Snot pada Ayam Broiler di Kabupaten Kupang Elisabet Tangkonda; Antin Yeftanti Nugrahening Widi; Bergita Soge
Jurnal Sain Veteriner Vol 41, No 1 (2023): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.75848

Abstract

Snot adalah salah satu gejala infeksi saluran pernafasan atas pada ayam, yang biasanya ditandai oleh kesulitan bernafas, eksudat dari rongga hidung, pembengkakan sinus infraorbital, ngorok, dan bersin. Meskipun seringkali dihubungkan dengan snot, tidak semua kejadian penyakit dengan gejala snot disebabkan oleh Avibaterium paragallinarum. Penyakit unggas dengan gejala snot sulit untuk diberantas karena manajemen peternakan yang kurang baik, variasi umur unggas dalam peternakan yang sama, fluktuasi suhu, dan kelembaban yang lebih tinggi, seperti yang sering ditemui Indonesia. Penyakit unggas dengan gejala snot telah dilaporkan dari berbagai provinsi di Indonesia. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri penyebab snot pada ayam broiler di Kabupaten Kupang. Sebanyak 4 sampel swab eksudat sinus infraorbital dan trakea dari unggas yang menunjukkan gejala khas snot diambil dari 2 peternakan broiler komersial di Kabupaten Kupang, dikultur pada agar darah dan agar coklat (disiapkan dengan 5% darah domba), dan diinkubasi pada suhu 37ºC selama 18-24 jam pada kondisi aerob dan anaerob. Sejumlah 23 isolat dimurnikan pada media nutrient agar kemudian dilanjutkan dengan untuk uji biokimia (katalase, oksidase, uji motilitas dan produksi indol) dan uji fermentasi gula. Hasil pengamatan menunjukkan, dari 23 isolat yang diuji, 17 isolat (73,91%) teridentifikasi sebagai Avibacterium paragallinarum (4 isolat), Ornithobacterium rhinotracheale (4 isolat), Pasteurella multocida (3 isolat) dan Mycoplasma sp. (6 isolat), sedangkan 6 isolat (26,09%) tidak teridentifikasi.. Dapat disimpulkan bahwa Avibacterium paragallinarum, Ornithobacterium rhinotracheale, Pasteurella multocida, dan Mycoplasma sp adalah penyebab gejala snot pada ayam broiler di Kabupaten Kupang.
Co-Authors Agnesia Endang Tri Hastuti Wahyuni Andriani Annytha Ina Rohi Detha Antin Yeftanti Nugrahening Widi Bambang Pontjo Priosoeryanto Bergita Soge Bili, Feny A. L. Cahyaningtyas, Dinda Eri Charles Rangga Tabbu Charles Rangga Tabu Denny Widaya Lukman Deta, Herlina Umbu Dewi Gaina, Cynthia Diana A. Wuri Diana A. Wuri Eky Melyani Sanu Filphin Adolfin Amalo Gaina, Cynthia D Gaina, Cynthia Dewi Herwin Pisestyani Jampur, Sesarius Wahyu Pagung Joesoef, Jayusman Arsiyanti Jois M. Jacob, Jois M. Kale, Maria Laurenci Fanny Permata Kallau, Novalino Harold Geoffrey Larry R.W Toha Laut, Meity M Loe, Fadhy Risckhy Loe, Fhady R. Loe, Fhady Risckhy Luji, Jovan Adiarsa Maha, Inggrid Trinidad Mandala, Graziela Angelicha Maria Aega Gelolodo Marlin Cindy Claudya Malelak Maxs U. E Sana Maxs Urias Ebenheizer Sanam Maya Shofa Meiko Kubo Murni, Theresia F. I. M. D. Naoaki Misawa Naoaki Misawai Ngadha L., Maria Emerentiana Ngasi, Bernadete Dwiyuni Novalino H. G Kallau Novian, Dede Rival Primatika, Roza Puspita, Anggi Putra Nugroho, Mega Perkasa Redempta Wea Roza Azizah Primatika, Roza Azizah Salestin, Ledy Chehfzy Sanam, Viona Mariana Dewi Satomi Sasaki Satoshi Sekiguchi Satria K. Frans Selan, Yulfia Nellymalik Simarmata, Yohanes T. R. M. R. Simarmata, Yohanes Timbun Raja Mangihut Ronael Soge, Bergitha Surachmi Setyaninigsih Takako Taniguchi Trinidad Maha, Inggrid Weakalla, Eugenia Raflesia Anjali Yasinta Widi, Antin Y. N. Widi, Antin Yeftanti Anggraeni Widodo Suwito Wuhan, Yustinus Oswin Primajuni Yeremia Yobellano Sitompul Yonas F Riwu Yulfia Nelymalik Selan, Yulfia Nelymalik