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All Journal Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences
T Syafriati
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Veterinary

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Rabies in Indonesia and The Development of Its:Diagnostic Techniques Adjid, R M A; Sarosa, A; Syafriati, T; ., Yuningsih
Indonesian Bulletin of Animal and Veterinary Sciences Vol 15, No 4 (2005)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (855.845 KB) | DOI: 10.14334/wartazoa.v15i4.816

Abstract

Rabies is a zoonotic disease which is crucial for public health, as it can infect human beings and causes death . Rabies has spread across the world including Indonesia . Control to erradicate rabies still faces many obstacles. Rabies research at Research Institute for Veterinary Science emphasizes on rabies erradication focussing on some aspects of diagnostic techniques. Studies on the spesimen preservation, the shipment to the laboratory and the collection of brain specimens by straw method as well as the use of cornea touch preparat as intra vitam specimens have been conducted. Rabies diagnostic techniques by NPLA or modified FAVN on multispot slides and their applications in the field have also been studied. This paper also describes the preparation of FITC conjugate. ELISA technique was developed for serological test but still need to be improved to reduce non specific reaction, while RREID was used for antigen detection. Rabies diagnostic was conducted by using RT-PCR but its application was very difficult to be done for intra vitam. Research on Croton tiglium and Pangium edule extracts as an alternative for the strychnine poison used for dog elimination in the field has also been conducted but further research is needed in the future. Key words: Rabies, zoonosis, research, diagnostic
Anticipating the Emerging of Some Strategical Infectious Animal Diseases in Indonesia Related to The Effect of Global Warming and Climate Change Bahri, Sjamsul; Syafriati, T
Indonesian Bulletin of Animal and Veterinary Sciences Vol 21, No 1 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.107 KB) | DOI: 10.14334/wartazoa.v21i1.951

Abstract

The effect of global warming and climate change is changing the season, included flooding in one area and very dry in other area, changing the temperature and humidity. These changes will trigger changing of the life of biological agent (virus, bacteria, parasites and so on), variety of animal species, variety of vectors as reservoir host of animal with the role of transmitting the disease to other animal species, This condition will trigger the new animal disease (emerging disease) or old disease will be re-emerged (re-emerging diseases). This paper will discuss the effect of global warming and climate change on animal diseases in Indonesia such as Bluetongue (BT), Nipah, Japanese encephalitis (JE), West Nile (WN), and Rift Valley fever (RVF). The climate changes such as increasing the earth temperature and rainfall will cause extremely increase of vector population for BT, JE, WN and RVF. In addition, animal transportation and bird migration from one country to others or region will cause changing of ecological system and will open the chance to distribute the diseases. Hence, anticipation on those disease outbreaks should be taken by conducting the surveilance and early detection to those diseases. The possibility of entering Nipah disease in Indonesia should be anticipated because the avaibility of Nipah virus and the reservoir host (Pteropus spp) and also pigs as amplifier host in the surrounding area. Other diseases such as, leptospirosis, anthrax and avian influenza (H5N1) are also have a wider potential to distributing the disease related to the climate change in Indonesia. Key words: Global warming, climate change, zoonotic disease
Development inactivated vaccine prototype of avian influenza (AI) H5N1 local isolate and its application at laboratory level Indriani, R; Dharmayanti, N.L.P.I; Syafriati, T; Wiyono, A; Adjid, R.M.A
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 4 (2005)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.851 KB) | DOI: 10.14334/jitv.v10i4.458

Abstract

A preliminary study related on vaccine safety and vaccination effectivity for controlling avian influenza (AI) subtype H5N1 was carried out at Virology Laboratorium, Indonesian Veteriner Institute, Bogor. A Prototype of inactivated vaccine was made using AI H5N1 local isolate (A/Chicken/West Java/67-2/2003). The vaccine was then tested for safety and protection in DOC of layers. Antibody response, protection and shedding virus challenge were observed in the experiment. Result showed that the vaccine was saved and protected against virulent viral challenge. Efective vaccination was achieved at 3 weeks chicken old started with low level of antibody. Antibody titre increased gradually and reached the top at 8 weeks post vaccination. Challenge test using AI virulent at the age of 4 and 8 weeks post vaccination showed that the vaccine gave high protection (90%). Viral shedding was not longer expressed than 7 days after challenge. It is concluded that this prototype is a satisfied AI vaccine in laboratory level.     Key Words: Vaccine, Avian Influenza, H5N1, HPAI
Isolation and characterization of virus of highly pathogenic avian influenza H5 subtype of chicken from outbreaks in Indonesia Wiyono, Agus; Indriani, R; Dhamaryanti, N.L.P.I; Damayanti, R; Parede, L; Syafriati, T; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 1 (2004)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (515.934 KB) | DOI: 10.14334/jitv.v9i1.429

Abstract

A study on the isolation and characterization of Highly Pathogenic Avian Influenza of chicken from outbreaks in Indonesia was conducted at Indonesian Research Institute for Veterinary Science. Outbreaks of avian disease had been reported in Indonesia since August 2003 affecting commercial layer, broiler, quail, and ostrich and also native chicken with showing clinical signs such as cyanosis of wattle and comb, nasal discharges and hypersalivation, subcutaneous ptechiae on foot and leg, diarre and sudden high mortality. The aim of this study is to isolate and characterize the causal agent of the disease. Samples of serum, feather follicle, tracheal swab, as well as organs of proventriculus, intestine, caecal tonsil, trachea and lungs were collected from infected animals. Serum samples were tested haemaglutination/haemaglutination inhibition to Newcastle Disease and Egg Drop Syndrome viruses. Isolation of virus of the causal agent of the outbreak was conducted from samples of feather follicle, tracheal swab, and organs using 11 days old specific pathogen free (SPF) embryonated eggs. The isolated viruses were then characterised by agar gel precipitation test using swine influenza reference antisera, by haemaglutination inhibition using H1 to H15 reference antisera, and by electron microscope examination. The pathogenicity of the viruses was confirmed by intravenous pathogenicity index test and its culture in Chicken Embryo Fibroblast primary cell culture without addition of trypsin. The study revealed that the causative agent of the outbreaks of avian disease in Indonesia was avian influenza H5 subtype virus based upon serological tests, virus isolation and characterization using swine influenza reference antisera, and electron microscope examination. While subtyping of the viruses using H1 to H15 reference antisera suggested that the virus is very likely to be an avian influenza H5N1 subtype virus. The pathogenicity test confirmed that the viruses are highly pathogenic to experimental animals. It is concluded that the causative agent of the outbreaks of avian disease in Indonesia was avian influenza H5 subtype virus. The result has been the basis of further study such as development serological tests and vaccine production. The decission of Indonesian Government to conduct vaccination program using homolog vaccine in order to control the disease is regarded as the correct choice. However, it should be accompanied by conducting surveillance and monitoring of the disease as well as the possibility of mutation of virus. The program should be coordinated nationally.   Key words: Virus isolation, characterization, chicken, outbreak, highly pathogenic avian influenza (HPAI), H5 subtype, Indonesia
Stunting or runting syndrome in broiler chickens and its pathological changes Wahyuwardani, S; Sani, Y; Parede, L; Syafriati, T; Poeloengan, M
Indonesian Journal of Animal and Veterinary Sciences Vol 5, No 2 (2000)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.386 KB) | DOI: 10.14334/jitv.v5i2.209

Abstract

A disease with stunting or runting syndrome in broiler chickens was investigated in 13 districts of West Java and Central Java provinces. A total of 291 chickens samples both with clinically stunted or runted were collected from 37 poultry farms. Blood samples were collected randomly from chickens in poultry farms for packed cells volume analysis. Tissues of liver, spleen, thymus, proventriculus, ventriculus intestines, caecum, pancreas, bursa fabricious and heart were collected for histopathological examination. Field surveys showed that prevalence rate of stunting or runting syndrome was varied from one farm to others between 0,1% to 50%. Clinical signs were noted as ununiformity of body size in a flock of chicken, stunted and/or runted of body weight gain and protrude of wing feather. Pathologic changes were hyperemic thymus, athropic thymus and athropic pancreas. While microscopically included dilatation crypt of Lieberkuhn, inflammation of thymus, pancreatitis and enteritis variably among each locations. The PCV level did not show direct link to the affected stunting or runting syndrome.   Key words: Syndrome, stunting, runting, pathology anatomy, histopathology
Stunting or runting syndrome in broiler chickens and its pathological changes S Wahyuwardani; Y Sani; L Parede; T Syafriati; M Poeloengan
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 2 (2000): JUNE 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.386 KB) | DOI: 10.14334/jitv.v5i2.209

Abstract

A disease with stunting or runting syndrome in broiler chickens was investigated in 13 districts of West Java and Central Java provinces. A total of 291 chicken's samples both with clinically stunted or runted were collected from 37 poultry farms. Blood samples were collected randomly from chickens in poultry farms for packed cells volume analysis. Tissues of liver, spleen, thymus, proventriculus, ventriculus intestines, caecum, pancreas, bursa fabricious and heart were collected for histopathological examination. Field surveys showed that prevalence rate of stunting or runting syndrome was varied from one farm to others between 0,1% to 50%. Clinical signs were noted as ununiformity of body size in a flock of chicken, stunted and/or runted of body weight gain and protrude of wing feather. Pathologic changes were hyperemic thymus, athropic thymus and athropic pancreas. While microscopically included dilatation crypt of Lieberkuhn, inflammation of thymus, pancreatitis and enteritis variably among each locations. The PCV level did not show direct link to the affected stunting or runting syndrome.   Key words: Syndrome, stunting, runting, pathology anatomy, histopathology
Isolation and characterization of virus of highly pathogenic avian influenza H5 subtype of chicken from outbreaks in Indonesia Agus Wiyono; R Indriani; N.L.P.I Dhamaryanti; R Damayanti; L Parede; T Syafriati; Darminto .
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 1 (2004): MARCH 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (515.934 KB) | DOI: 10.14334/jitv.v9i1.429

Abstract

A study on the isolation and characterization of Highly Pathogenic Avian Influenza of chicken from outbreaks in Indonesia was conducted at Indonesian Research Institute for Veterinary Science. Outbreaks of avian disease had been reported in Indonesia since August 2003 affecting commercial layer, broiler, quail, and ostrich and also native chicken with showing clinical signs such as cyanosis of wattle and comb, nasal discharges and hypersalivation, subcutaneous ptechiae on foot and leg, diarre and sudden high mortality. The aim of this study is to isolate and characterize the causal agent of the disease. Samples of serum, feather follicle, tracheal swab, as well as organs of proventriculus, intestine, caecal tonsil, trachea and lungs were collected from infected animals. Serum samples were tested haemaglutination/haemaglutination inhibition to Newcastle Disease and Egg Drop Syndrome viruses. Isolation of virus of the causal agent of the outbreak was conducted from samples of feather follicle, tracheal swab, and organs using 11 days old specific pathogen free (SPF) embryonated eggs. The isolated viruses were then characterised by agar gel precipitation test using swine influenza reference antisera, by haemaglutination inhibition using H1 to H15 reference antisera, and by electron microscope examination. The pathogenicity of the viruses was confirmed by intravenous pathogenicity index test and its culture in Chicken Embryo Fibroblast primary cell culture without addition of trypsin. The study revealed that the causative agent of the outbreaks of avian disease in Indonesia was avian influenza H5 subtype virus based upon serological tests, virus isolation and characterization using swine influenza reference antisera, and electron microscope examination. While subtyping of the viruses using H1 to H15 reference antisera suggested that the virus is very likely to be an avian influenza H5N1 subtype virus. The pathogenicity test confirmed that the viruses are highly pathogenic to experimental animals. It is concluded that the causative agent of the outbreaks of avian disease in Indonesia was avian influenza H5 subtype virus. The result has been the basis of further study such as development serological tests and vaccine production. The decission of Indonesian Government to conduct vaccination program using homolog vaccine in order to control the disease is regarded as the correct choice. However, it should be accompanied by conducting surveillance and monitoring of the disease as well as the possibility of mutation of virus. The program should be coordinated nationally.   Key words: Virus isolation, characterization, chicken, outbreak, highly pathogenic avian influenza (HPAI), H5 subtype, Indonesia
Development inactivated vaccine prototype of avian influenza (AI) H5N1 local isolate and its application at laboratory level R Indriani; N.L.P.I Dharmayanti; T Syafriati; A Wiyono; R.M.A Adjid
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 4 (2005): DECEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.851 KB) | DOI: 10.14334/jitv.v10i4.458

Abstract

A preliminary study related on vaccine safety and vaccination effectivity for controlling avian influenza (AI) subtype H5N1 was carried out at Virology Laboratorium, Indonesian Veteriner Institute, Bogor. A Prototype of inactivated vaccine was made using AI H5N1 local isolate (A/Chicken/West Java/67-2/2003). The vaccine was then tested for safety and protection in DOC of layers. Antibody response, protection and shedding virus challenge were observed in the experiment. Result showed that the vaccine was saved and protected against virulent viral challenge. Efective vaccination was achieved at 3 weeks chicken old started with low level of antibody. Antibody titre increased gradually and reached the top at 8 weeks post vaccination. Challenge test using AI virulent at the age of 4 and 8 weeks post vaccination showed that the vaccine gave high protection (90%). Viral shedding was not longer expressed than 7 days after challenge. It is concluded that this prototype is a satisfied AI vaccine in laboratory level.     Key Words: Vaccine, Avian Influenza, H5N1, HPAI
Rabies in Indonesia and The Development of Its:Diagnostic Techniques R M A Adjid; A Sarosa; T Syafriati; Yuningsih .
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 15, No 4 (2005): DECEMBER 2005
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (855.845 KB) | DOI: 10.14334/wartazoa.v15i4.816

Abstract

Rabies is a zoonotic disease which is crucial for public health, as it can infect human beings and causes death . Rabies has spread across the world including Indonesia . Control to erradicate rabies still faces many obstacles. Rabies research at Research Institute for Veterinary Science emphasizes on rabies erradication focussing on some aspects of diagnostic techniques. Studies on the spesimen preservation, the shipment to the laboratory and the collection of brain specimens by straw method as well as the use of cornea touch preparat as intra vitam specimens have been conducted. Rabies diagnostic techniques by NPLA or modified FAVN on multispot slides and their applications in the field have also been studied. This paper also describes the preparation of FITC conjugate. ELISA technique was developed for serological test but still need to be improved to reduce non specific reaction, while RREID was used for antigen detection. Rabies diagnostic was conducted by using RT-PCR but its application was very difficult to be done for intra vitam. Research on Croton tiglium and Pangium edule extracts as an alternative for the strychnine poison used for dog elimination in the field has also been conducted but further research is needed in the future. Key words: Rabies, zoonosis, research, diagnostic
Anticipating the Emerging of Some Strategical Infectious Animal Diseases in Indonesia Related to The Effect of Global Warming and Climate Change Sjamsul Bahri; T Syafriati
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 21, No 1 (2011): MARCH 2011
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.107 KB) | DOI: 10.14334/wartazoa.v21i1.951

Abstract

The effect of global warming and climate change is changing the season, included flooding in one area and very dry in other area, changing the temperature and humidity. These changes will trigger changing of the life of biological agent (virus, bacteria, parasites and so on), variety of animal species, variety of vectors as reservoir host of animal with the role of transmitting the disease to other animal species, This condition will trigger the new animal disease (emerging disease) or old disease will be re-emerged (re-emerging diseases). This paper will discuss the effect of global warming and climate change on animal diseases in Indonesia such as Bluetongue (BT), Nipah, Japanese encephalitis (JE), West Nile (WN), and Rift Valley fever (RVF). The climate changes such as increasing the earth temperature and rainfall will cause extremely increase of vector population for BT, JE, WN and RVF. In addition, animal transportation and bird migration from one country to others or region will cause changing of ecological system and will open the chance to distribute the diseases. Hence, anticipation on those disease outbreaks should be taken by conducting the surveilance and early detection to those diseases. The possibility of entering Nipah disease in Indonesia should be anticipated because the avaibility of Nipah virus and the reservoir host (Pteropus spp) and also pigs as amplifier host in the surrounding area. Other diseases such as, leptospirosis, anthrax and avian influenza (H5N1) are also have a wider potential to distributing the disease related to the climate change in Indonesia. Key words: Global warming, climate change, zoonotic disease
Co-Authors A Sarosa A Wiyono Agus Wiyono Darminto . Darminto . L Parede M Poeloengan N.L.P.I Dhamaryanti N.L.P.I Dharmayanti R Damayanti R Indriani R M A Adjid R.M.A Adjid S Wahyuwardani Sjamsul Bahri Sjamsul Bahri Y Sani Yuningsih .