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All Journal Jurnal Penelitian Saintek Jurnal Ilmiah Farmasi JPS Unimus Media Ilmu Kesehatan Al-Tamimi Kesmas: Jurnal Ilmu Kesehatan Masyarakat (Journal of Public Health Sciences) Al-Asalmiya Nursing: Journal of Nursing Sciences Jurnal Kesehatan Saintika Meditory Journal of Fundamental and Applied Pharmaceutical Science Action Research Literate (ARL) Jurnal Ilmu Kesehatan Bhakti Setya Medika
burhan, Amelia handayani
Politeknik Kesehatan Bhakti Setya Indonesia, Yogyakarta, Indonesia
Chemistry Decision Sciences, Operations Research & Management Education Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Effect of soaking Allium sativum peel waste on microbial contamination of salted anchovy rice and analysis of its total phenolic content Iramie Duma Kencana Irianto; Amelia Handayani Burhan; Afifah Astuti
Jurnal Penelitian Saintek Vol 31, No 1 (2026)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v30i1.55677

Abstract

Approximately 84% of salted anchovy rice fish sold in traditional markets in Yogyakarta still contain formaldehyde, a hazardous substance that may cause adverse health effects ranging from throat irritation to cancer. Saponin compounds contained in Allium sativum peel waste have been reported to reduce formaldehyde levels in salted anchovy rice fish by up to 89.12%; however, the reduction of formaldehyde may increase microbial contamination. This study aimed to examine microbial contamination in salted anchovy rice fish after immersion treatment using A. sativum peel waste. Qualitative analysis of saponins was conducted using the Froth test and Thin Layer Chromatography (TLC), while phenolic compounds were analyzed qualitatively using TLC and quantitatively using spectrophotometric methods. Microbial contamination was evaluated using the Total Plate Count (TPC) method. The results demonstrated that A. sativum peel waste contained saponin and phenolic compounds, with an average phenolic content of 8.57 ppm. Furthermore, 1 g of A. sativum peel waste reduced microbial contamination in salted anchovy rice fish by up to 10,000-fold. The microbial contamination level showed a TPC value of 8.55 × 10³ colonies/g, indicating that the product remained within the safe consumption limit of less than 1 × 10⁵ colonies/g.
Co-Authors Aditya Fitriasari Afifah Astuti Aini, Rahma Nur Ana Mardiyaningsih Astuti, Afifah Azizah, Shyella Bintoro, Danang Wahyu Farisya Nurhaeni Farisya Nurhaeni Fydika Nurul Riani Sumarjo Ibnu Mardiyoko Indraswari, Laksita Putri Iramie Duma Kencana Irianto Kencana Irianto, Iramie Duma Munawaroh, Umu Azizah Pradah, Wiby Maliya Puji, Rian Purwanto Purwanto Puspito R, Yuli Putri, Salsabilla Surya Rahmatullah, Widia Saputri, Lintang Handoyo Sulaiman, Fadia Wijaya, Putri Windadari Murni Hartini Yuli Puspito R Yuli Puspito Rini, Yuli Puspito