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All Journal Jurnal Kesehatan Reproduksi The Indonesian Biomedical Journal Jurnal Keperawatan Padjadjaran Jurnal Kesehatan Reproduksi JURNAL ETIKA KEDOKTERAN INDONESIA Mandala of Health : A Scientific Journal
Shofwal Widad
Department Of Obstetrics And Gynecology, Faculty Of Medicine, Public Health, And Nursing, Universitas Gajah Mada – Dr. Sardjito General Hospital, Jl. Sekip Utara, Yogyakarta 55281
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : The Indonesian Biomedical Journal

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High Expression of PR-A and Low Expression of PR-B is Correlated with Inflammation in Endometrioma Cases Yuane, Erick; Dewanto, Agung; Widad, Shofwal
The Indonesian Biomedical Journal Vol 15, No 1 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i1.2114

Abstract

BACKGROUND: Progestin therapy has been commonly used in endometriosis. The regulation of progesterone receptors B (PR-B) greatly affects the success rate of therapy in cases of endometriosis. The presence of tumor necrosis factor (TNF)-a in endometriosis triggers PR-B hypermethylation, decreasing PR-B expression and PR-B/A ratio that induce progesterone resistance. It may also occur in endometrioma. Studies regarding the distribution of PR-A and PR-B with TNF-a expression in endometriosis with endometrioma tissue samples has not been elucidated well. Therefore, this study was conducted to measure and compare the distribution of PR-A and PR-B expression, and to assess the effect of PR-B/A ratio on TNF-a in endometrioma and benign cysts.METHODS: A cross-sectional study was conducted by collecting paraffin blocks of endometriomas and benign cysts as controls, from patients undergoing surgery at Dr. Sardjito Hospital, Yogyakarta. Immunohistochemistry was performed to assess the expressions of PR-A, PR-B, TNF-a and PR-B/A ratio, to compared differences between endometriomas and benign cysts.RESULTS: Twenty-three endometrioma and 22 benign cyst tissue samples were collected. The mean PR-B expression and PR-B/A ratio were found to be lower in endometriomas than benign cysts, and mean expression of PR-A and TNF-a in endometriomas was higher than in benign cysts. However, there were no significant correlations between the expression of PR-A, PR-B, PR-B/A ratio, and TNF-a with endometriosis severity.CONCLUSION: In endometrioma cases, the expression of PR-A and TNF-a was higher, while the expression of PR-B and PR-B/A ratio was lower. However, there was no significant relationship between the ratio of PR-B/A and TNF-a.KEYWORDS: progesterone receptor, tumor necrosis factor-alpha, endometriosis, endometrioma, benign cyst, ovarian cyst
MMP-9 and TIMP-1 Promote Extracellular Matrix Remodeling in the Formation of Ovarian Endometrioma: in vitro Study on Chicken Chorioallantoic Membrane Sari, Vidia; Jenie, Riris Istighfari; Widad, Shofwal; Dewanto, Agung
The Indonesian Biomedical Journal Vol 15, No 1 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i1.2090

Abstract

BACKGROUND: The invasion of endometrial tissue in the peritoneal layers is an important precursor to the formation of endometriosis. However, the mechanisms of the initial development of endometrioma remain unclear. This study aimed to explore the correlation between matrix metalloproteinase (MMP)-9 activity and tissue inhibitors of metalloproteinase (TIMP)-1 expression in the initial development of endometriosis through the invasion process of endometrial lesions in extracellular matrix (ECM) remodeling.METHODS: A total of 30 samples of endometrioma tissue were obtained from 24 women with endometriosis and examined at Dr. Sardjito General Hospital, Yogyakarta, Indonesia. The tissue was then implanted into a chicken chorioallantoic membrane (CAM) for five days to examine its invasion ability. The invasion scores were assessed using hematoxylin-eosin staining. MMP-9 activity and TIMP-1 expression were analyzed using gelatin zymography and western blot, respectively. The correlations between MMP-9 activity and TIMP-1 expressions with the invasion scores were analyzed using Spearman correlation tests with significance set as p<0.05.RESULTS: The results showed that there was a strong positive correlation between MMP-9 activity and invasion scores of endometriomas tissue (r=0.656; p=0.000). Meanwhile, expression levels of TIMP-1 had a weak negative correlation with the increase of invasion scores of endometrioma (r=-0.388; p=0.034). These results indicate the involvement of MMP-9 and TIMP-1 in the initial development of endometriosis during the ECM remodeling.CONCLUSION: MMP-9 activity and TIMP-1 expression were correlated with the invasion score of endometrioma tissue. This study provides evidence for understanding the mechanisms involved in ECM remodeling in the early process of endometriosis.KEYWORDS: endometrioma, extracellular matrix remodeling, MMP-9, TIMP-1 
Co-Authors Addin Trirahmanto Agung Dewanto Agung Dewanto Agung Dewanto Agung Kurniawan Agung Kurniawan Ahmad Muzakky Ahsanudin Attamimi Anis Widyasari Arief Boediono Ayuandari, Sarrah Bayu Rizky Prabowo Carla Marchira Daniel Happy Putra Dewanto, Agung Dhestiana, Mega Diah Rumekti Hadiati Diany Ayu Suryaningtyas Djaswadi Dasuki Djaswadi Dasuki Dwi Haryadi Eda, Maria Katharina Edi Patmini Setya Siswanti Edy Priyanto Elsi Dwi Hapsari Farida Indriani Farida Indriani Fatmawati, Rina Galuh Dyah Fatmala H.M, Irwan Muhaeimin Hanifah Mirzanie Ihsan Yudhitama Irwan Taufiqur Rahman, Irwan Taufiqur Ita Fauzia Hanoum Ita Fauzia Hanoum Ita Fauzia Hanoum, Ita Fauzia M. Lutfi M. Nurhadi Rahman Marchira, Carla Maria Katharina Eda Martha Chaterince Silitonga Mega Dhestiana Mirzanie, Hanifah Moh Nailul Fahmi MUHAMMAD LUTFI Muhammad Lutfi Mulyoto Pangestu MULYOTO PANGESTU Novita, Devie Ova Emilia Permana, Ridho Prakoso Adhi Wibowo R. Sapto Hendri Boedi Soesatyo Rahman, M. Nurhadi Rathi Manjari Fauziah Rathi Manjari Fauziah Ridho Permana Rina Fatmawati Riris Istighfari Jenie Rukmono Siswishanto Sari, Vidia Shinta Prawitasari Silitonga, Martha Chaterince Siswanti, Edi Patmini Setya Sulchan Sofoewan Sulchan Sofoewan, Sulchan Wibowo, Prakoso Adhi Yanita Trisetiyaningsih Yasmina Ema Yasmina Ema Yuane, Erick Yundari, Yundari