Garuda - Garba Rujukan Digital

MICROVOLUME OF 0.1ÂµL GAMA SLEEVED CRYOLOOPS FOR BLASTOCYST VITRIFICATION OF ASSISTED REPRODUCTIVE TECHNOLOGY PATIENTS Hanoum, Ita Fauzia; Boediono, Arief; Pangestu, Mulyoto; Haryadi, Dwi; Widad, Shofwal; Dasuki, Djaswadi
JURNAL KESEHATAN REPRODUKSI Vol 2, No 1 (2015)
Publisher : IPAKESPRO

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.875 KB)

Ita Fauzia Hanoum1,2, Arief Boediono3, Mulyoto Pangestu4,5, Dwi Haryadi1,Shofwal Widad1,2, Djaswadi Dasuki1,2Â ABSTRAKÂ Latar Belakang: Prosedur embrio vitrifikasi menggunakan alat berupa grid, straw atau cryoloop. Gama Sleeved cryoloop dibuat dan dikembangkan di klinik Permata Hati. Untuk itu, dilakukan pengamatan keberhasilan prosedur vitrifikasi menggunakan 0.1Âµl Gama Sleeved cryoloop.Metode: Vitrifikasi dilakukan pada blastokis dengan kualitas baik yang diperoleh pada hari ke 5 setelah fertilisasi. Inform consent telah disampaikan sebelumnya kepada pasien program bayi tabung di Klinik Permata Hati. Prosedur dilakukan dengan menggunakan media handling (GMOPS Plus; Vitrolife) embrio diinkubasi selama 1 menit; (7.5% EG (v/v); 7.5% DMSO (v/v)) selama 2-3 menit, (15% EG (v/v); 15% DMSO v/v; 10 mg/ml Ficoll; 0.65 M Sucrosa) selama 30 detik pada suhu ruang sebelum kemudian diletakkan di dalam cryoloop, setelah itu secara cepat cryoloop yang berisi embrio dibenamkan ke dalam nitrogen cair. Sebelum dilakukan embryo transfer (ET), embrio dihangatkan dengan cara two step technique (sucrose 0.25M) selama 2 menit dan selama 3 menit (sucrose 0.125M).Hasil: Sejumlah 97 blastokis divitrifikasi dan dihangatkan (67 pasien), dimana 91 blastokis berhasil ditransfer ke rahim ibu (93.8%). Blastokis yang tidak berhasil selamat dari prosedur penghangatan adalah blastokis dengan kerusakan lebih dari 50%. Diperoleh kehamilan klinis 43.3% sedangkan angka implantasi adalah 37.4%. Sampai saat ini, dilaporkan 20 kelahiran (23 bayi) dari program vitrifikasi menggunakan 0.1Âµl Gama Sleeved cryoloop, sementara 5 kehamilan masih berlangsung. Satu kehamilan dilaporkan gugur pada usia kehamilan yang masih sangat awal, dua keguguran pada usia kehamilan 12 minggu dan satu bayi lahir meninggal karena kelainan kongenital.Kesimpulan: 0.1Âµl Gama Sleeved cryoloop merupakan pilihan untuk digunakan sebagai alat vitrifikasi blastokis. Data awal yang kami sampaikan dan kelahiran bayi dari program tersebut memberikan harapan untuk kesuksesan program simpan beku embrio di klinik Permata Hati RSUP DR Sardjito Yogyakarta.Kata kunci: kriopreservasi, blastokis, vitrifikasiÂ ABSTRACTBackground: Vitrification has been applied succesfully in human embryo using grid, straw and cryoloop. Gama Sleeved is a home made device develop at Permata Hati. We assessed the survival rate of human blastocyst vitrified in 0.1Âµl Gama Sleeved cryoloop as device.Method: Excess good grade human D5 embryos were vitrified, upon a detailed informed consent. Embryos were hold in handling media (GMOPS Plus; Vitrolife) for 1 minute; (7.5% EG (v/v); 7.5% DMSO (v/v)) for 2-3 minutes, (15% EG (v/v); 15% DMSO v/v; 10 mg/ml Ficoll; 0.65 M Sucrosa) for 30 seconds at room temperature before inserted in to the loops, then directly plunged into the liquid nitrogen. Prior to ET, embryos were warmed by two step technique in sucrose 0.25M for 2 min and 0.125M sucrosa for 3 min. Embryos were then cultured.Results: Total of 97 vitrified warmed human blastocyst (67 patients) were used and 91 (93.8%) were transferred. Non-transferred blastocyst (6.2%) has more than 50% lyse. The clinical pregnancy rate was 43.9%. The implantation rate was 37.4%. Currently, 20 deliveries of 23 babies born from vitrified blastocyst using 0.1Âµl Gama Sleeved cryoloop, and another 5 ongoing pregnancy. So far there was 1 early pregnancy loss, 2 miscarriages at 12 weeks pregnancy, and one infant died due to a congenital anomaly.Conclusion: 0.1Âµl Gama Sleeved cryoloop provides an excellent alternative to existing vitrification devices. These initial data and babies delivered from the program have been promising to a vitrification system in our own ART program.Keywords: cryopreservation, blastocyst, vitrification1Permata Hati Infertility Clinic RSUP DR Sardjito, Yogyakarta2Div Reproductive Endocrinology and Fertility OBGYN Medical Faculty Gadjah Mada University, Yogyakarta3Lab. Anatomi Embriologi FKH, Institut Teknologi Pertanian, Bogor4EPRD- Dept. Obstetrics and Gynecology, Monash University, Monash Medical Center,Victoria, Melbourne5Lab. Reproductive Physiology, Jenderal Soedirman University, PurwokertoÂ Correspondence address: + 62 274 518684; fax + 62 274 553575; email: itafauzia@yahoo.com

MICROVOLUME OF 0.1ÂµL GAMA SLEEVED CRYOLOOPS FOR BLASTOCYST VITRIFICATION OF ASSISTED REPRODUCTIVE TECHNOLOGY PATIENTS Hanoum, Ita Fauzia; Boediono, Arief; Pangestu, Mulyoto; Haryadi, Dwi; Widad, Shofwal; Dasuki, Djaswadi
JURNAL KESEHATAN REPRODUKSI Vol 2, No 1 (2015)
Publisher : Fakultas Kedokteran, Kesehatan Masyarakat dan Keperawatan UGM

Ita Fauzia Hanoum1,2, Arief Boediono3, Mulyoto Pangestu4,5, Dwi Haryadi1,Shofwal Widad1,2, Djaswadi Dasuki1,2Â ABSTRAKÂ Latar Belakang: Prosedur embrio vitrifikasi menggunakan alat berupa grid, straw atau cryoloop. Gama Sleeved cryoloop dibuat dan dikembangkan di klinik Permata Hati. Untuk itu, dilakukan pengamatan keberhasilan prosedur vitrifikasi menggunakan 0.1Âµl Gama Sleeved cryoloop.Metode: Vitrifikasi dilakukan pada blastokis dengan kualitas baik yang diperoleh pada hari ke 5 setelah fertilisasi. Inform consent telah disampaikan sebelumnya kepada pasien program bayi tabung di Klinik Permata Hati. Prosedur dilakukan dengan menggunakan media handling (GMOPS Plus; Vitrolife) embrio diinkubasi selama 1 menit; (7.5% EG (v/v); 7.5% DMSO (v/v)) selama 2-3 menit, (15% EG (v/v); 15% DMSO v/v; 10 mg/ml Ficoll; 0.65 M Sucrosa) selama 30 detik pada suhu ruang sebelum kemudian diletakkan di dalam cryoloop, setelah itu secara cepat cryoloop yang berisi embrio dibenamkan ke dalam nitrogen cair. Sebelum dilakukan embryo transfer (ET), embrio dihangatkan dengan cara two step technique (sucrose 0.25M) selama 2 menit dan selama 3 menit (sucrose 0.125M).Hasil: Sejumlah 97 blastokis divitrifikasi dan dihangatkan (67 pasien), dimana 91 blastokis berhasil ditransfer ke rahim ibu (93.8%). Blastokis yang tidak berhasil selamat dari prosedur penghangatan adalah blastokis dengan kerusakan lebih dari 50%. Diperoleh kehamilan klinis 43.3% sedangkan angka implantasi adalah 37.4%. Sampai saat ini, dilaporkan 20 kelahiran (23 bayi) dari program vitrifikasi menggunakan 0.1Âµl Gama Sleeved cryoloop, sementara 5 kehamilan masih berlangsung. Satu kehamilan dilaporkan gugur pada usia kehamilan yang masih sangat awal, dua keguguran pada usia kehamilan 12 minggu dan satu bayi lahir meninggal karena kelainan kongenital.Kesimpulan: 0.1Âµl Gama Sleeved cryoloop merupakan pilihan untuk digunakan sebagai alat vitrifikasi blastokis. Data awal yang kami sampaikan dan kelahiran bayi dari program tersebut memberikan harapan untuk kesuksesan program simpan beku embrio di klinik Permata Hati RSUP DR Sardjito Yogyakarta.Kata kunci: kriopreservasi, blastokis, vitrifikasiÂ ABSTRACTBackground: Vitrification has been applied succesfully in human embryo using grid, straw and cryoloop. Gama Sleeved is a home made device develop at Permata Hati. We assessed the survival rate of human blastocyst vitrified in 0.1Âµl Gama Sleeved cryoloop as device.Method: Excess good grade human D5 embryos were vitrified, upon a detailed informed consent. Embryos were hold in handling media (GMOPS Plus; Vitrolife) for 1 minute; (7.5% EG (v/v); 7.5% DMSO (v/v)) for 2-3 minutes, (15% EG (v/v); 15% DMSO v/v; 10 mg/ml Ficoll; 0.65 M Sucrosa) for 30 seconds at room temperature before inserted in to the loops, then directly plunged into the liquid nitrogen. Prior to ET, embryos were warmed by two step technique in sucrose 0.25M for 2 min and 0.125M sucrosa for 3 min. Embryos were then cultured.Results: Total of 97 vitrified warmed human blastocyst (67 patients) were used and 91 (93.8%) were transferred. Non-transferred blastocyst (6.2%) has more than 50% lyse. The clinical pregnancy rate was 43.9%. The implantation rate was 37.4%. Currently, 20 deliveries of 23 babies born from vitrified blastocyst using 0.1Âµl Gama Sleeved cryoloop, and another 5 ongoing pregnancy. So far there was 1 early pregnancy loss, 2 miscarriages at 12 weeks pregnancy, and one infant died due to a congenital anomaly.Conclusion: 0.1Âµl Gama Sleeved cryoloop provides an excellent alternative to existing vitrification devices. These initial data and babies delivered from the program have been promising to a vitrification system in our own ART program.Keywords: cryopreservation, blastocyst, vitrification1Permata Hati Infertility Clinic RSUP DR Sardjito, Yogyakarta2Div Reproductive Endocrinology and Fertility OBGYN Medical Faculty Gadjah Mada University, Yogyakarta3Lab. Anatomi Embriologi FKH, Institut Teknologi Pertanian, Bogor4EPRD- Dept. Obstetrics and Gynecology, Monash University, Monash Medical Center,Victoria, Melbourne5Lab. Reproductive Physiology, Jenderal Soedirman University, PurwokertoÂ Correspondence address: + 62 274 518684; fax + 62 274 553575; email: itafauzia@yahoo.com

Jumlah Sel Piramidal CA3 Hipokampus Tikus Putih Jantan pada Berbagai Model Stres Kerja Kronik Arjadi, Fitrianto; Soejono, Sri Kadarsih; Maurits, Lientje Setyawati; Pangestu, Mulyoto
Majalah Kedokteran Bandung Vol 46, No 4 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (556.626 KB)

Paparan stres kronik dan berkepanjangan mengakibatkan hilangnya neuron di regioÂ CA3 (cornu ammonis) hipokampus danÂ penurunan kognitif. Tujuan penelitianÂ mengetahui perbedaan jumlah sel piramidal CA3 hipokampus tikus putih jantan pada model stres kerja meliputiÂ Â paradoxical sleep deprivation (PSD), imobilisasi, dan footshock kronik. Metode penelitian adalahÂ post-test only with control group design experimental dengan rancangan acak lengkapÂ menggunakan 24 ekor tikus putih jantan galur Wistar usia 3â€“4 bulan yang dibagi 4 kelompok:Â KI kontrol tanpa perlakuan,Â KII (stres PSD), KIII (stres imobilisasi), dan KIV (stres footshock) dan sel piramidal CA3 hipokampusÂ diwarnai dengan toluidine-blue. Jumlah sel dihitung menggunakanÂ perangkat lunak Image raster v2.1, perbesaran 400x tiap 10 lapangan pandang. Penelitian dilakukanÂ 6 bulan (Aprilâ€“September 2012) di Laboratorium Hewan Coba, Fakultas Kedokteran dan Ilmu Kesehatan Universitas Jenderal Soedirman. Analisis data menggunakan analysis of variance (ANOVA) dengan Post-Hoc least significant difference (LSD) menunjukkanÂ perbedaan jumlah sel piramidal CA3 hipokampus signifikan (p=0,037) pada minimal dua kelompok perlakuan. Uji statistik dengan Post-Hoc LSD menunjukkan perbedaan signifikan jumlah sel piramidal CA3 hipokampus antaraÂ kelompok kontrol (12,9Â±2,47) dan kelompok imobilisasi (9,00Â±1,53) (p<0,05). Simpulan, kelompok imobilisasi kronik memiliki jumlah sel piramidal CA3 hipokampus terendah dibandingkan dengan ketiga kelompok lainnya.Kata kunci:Â Model stres kerja kronik, sel piramidal CA3 hipokampus, tikus putih jantanNumber of CA3 Pyramidal Cell in Male Albino RatÂ Â Hippocampus Exposed to Various Chronic Work Stress ModelsÂ AbstractProlonged and chronic exposure to stress leads to the loss of neurons at the CA3 (cornu ammonis) hippocampus region and spatial memory deficits. The aim of this study was to study the number of CA3 pyramidal cells in albino rats that were exposed to chronic stress of works model consisting of paradoxical sleep deprivation (PSD), immobilization, and foot shock stresses. The method applied was the post-test only method with control group experimental design using completed randomized design (CRD on 24 3â€“4 month old male Wistar rats . The rats were divided into 4 groups: group I (control), group II (PSD stress), group III (immobilization stress), and group IV (footshock stress). The CA3 pyramidal cell hippocampus was stained with toluidine-blue. The number of CA3 pyramidal cell of hippocampus was counted using Image raster v2.1 software at 400x magnification in 10 duplicates for each sample. The study was conducted in six months (Aprilâ€“September 2012) at the Animal Laboratory, Faculty of Medical and Health Sciences, Jenderal Soedirman University. Analysis for the differences in the number of CA3 pyramidal cells was conducted using analysis of variance (ANOVA) with Post-Hoc LSD. The results of the ANOVAÂ showed a p value=0.037, meaning that there was significant difference in at least two groupsof treatment. Further statistical test using Post-Hoc LSD showed a significant differenceÂ between the control group (12.9 Â± 2.47) and the chronic immobillization group (9,00 Â± 1,53) (p<0.05). In conclusion, the chronic immobillization stress group has the lowest average number of hippocampus CA3 pyramidal cells compared to other groups Key words: CA3 pyramidal cell in hippocampus, chronic works stress model, male albino ratsÂ DOI: 10.15395/mkb.v46n4.337

Genealogy of Power in Sustainable Tourism Development in Mina Wisata Technopark Sleman Albizzia, Oktarina; Sakina, Aulia Widya; Pangestu, Mulyoto; Wedadjati, Ratna Sesotya
Jurnal Pemberdayaan Masyarakat: Media Pemikiran dan Dakwah Pembangunan Vol. 6 No. 2 (2022): Jurnal Pemberdayaan Masyarakat
Publisher : UIN Sunan Kalijaga, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/jpm.2022.062-03

This study aims to analyze the parties involved in innovation program and their interests, identifies the parties who benefit and lose, and determine the sustainability of the development of Mina Wisata Technopark Sleman. This research used qualitative methods with Foucaultian’s framework of the genealogy of power or knowledge. The construction of the dynamics of relations and power relations can be seen from the discourse on the power of the actors involved in the management, such as the central and local governments as planners at the executive and driving levels; the company as the main key in the tourism industry; local communities in the development area as program objects; as well as the Lurah and the Village Apparatus as implementation and supervisors of development. The dynamics of developing a mina wisata technopark resulted in the construction that the existence of a management agency was the result of communicative action through a long dialogue process. So, that power or authority can side with the weakest parties to build productive spaces and their networks in order to improve their welfare. Mina wisata technopark must be developed through the active role of the community in making development decisions in an open, democratic, and responsible manner (bottom up planning), trought synergy with all stakeholders.

Enhancing Reproductive Health and Sexually Transmitted Infection Prevention Awareness through Community Engagement Mustifah, Etty Farida; Ujianti, Irena; Sukarya, Wawang Setiawan; Pangestu, Mulyoto
Sanus Medical Journal Vol. 6 No. 2 (2024)
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/sanus.v6i2.18202

ntroduction The Thousand Islands, a popular tourist destination in DKI Jakarta Province, is facing a new challenge, namely the increasing concern about the transmission of Sexually Transmitted Infections (STIs) triggered by the increase in tourism visits. In response to this issue, a community service activity was carried out by the Faculty of Medicine at UHAMKA. Objective The aim of this activity is to raise awareness and knowledge in the community, particularly among educators in the Thousand Islands, about the dangers of and prevention against STIs and the importance of Reproductive Health Education. Method The partners in this community service activity are teachers in the Thousand Islands. A total of 30 teachers were involved in this training. The methods used involve training and counseling that incorporate direct training and open discussions. Data were collected through observations and field notes. Results The results of this activity include an increase in understanding and skills of teachers concerning Reproductive Health Education and STI prevention, which they can apply in educating students. Additionally, it was found that community awareness about the dangers of STIs also increased as a direct result of this activity. Conclusion The conclusion that can be derived from this activity is that appropriate education and training on reproductive health and the prevention of STIs for the community, specifically educators, can be an effective strategy in controlling STI transmission in tourist areas like the Thousand Islands. This activity also helps to strengthen the local health system in dealing with the challenge of STI transmission.

Terapi TB Paru Kasus Baru MTB Not Detected Disertai Peningkatan Fungsi Hati: Laporan Kasus Apriliana, Dian; Putri, Dewi Novita; Pangestu, Mulyoto
Sanus Medical Journal Vol. 6 No. 2 (2024)
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/sanus.v6i2.18201

Tuberculosis (TB) is caused by Mycobacterium tuberculosis and can infect various organs, including the lungs and extrapulmonary. Standard treatment uses anti-TB drugs (OAT) such as rifampicin, isoniazid, ethambutol, and pyrazinamide, which can cause hepatotoxic side effects or Drug-Induced Liver Injury (DILI). This study aims to analyze the incidence of DILI in TB patients receiving OAT FDC therapy. The method used is a case study on a 24-year-old patient who experienced nausea, vomiting, and jaundice after receiving FDC therapy. Laboratory examinations showed increased ALT and AST, indicating DILI. Data were analyzed with periodic monitoring of liver enzymes and clinical changes. The results showed that temporary cessation of OAT and administration of hepatoprotective curcuma supplements were effective in improving liver function. This study suggests routine liver enzyme examination in patients receiving OAT therapy to prevent DILI. Alternative OAT therapy can be considered in patients with severe DILI. Tuberkulosis (TBC) disebabkan oleh Mycobacterium tuberculosis dan dapat menginfeksi berbagai organ, termasuk paru dan ekstra paru. Pengobatan standar menggunakan obat anti-TBC (OAT) seperti rifampisin, isoniazid, ethambutol, dan pyrazinamid, yang dapat menyebabkan efek samping hepatotoksik atau Drug-Induced Liver Injury (DILI). Penelitian ini bertujuan untuk menganalisis kejadian DILI pada pasien TBC yang menerima terapi OAT FDC. Metode yang digunakan adalah studi kasus pada pasien berusia 24 tahun yang mengalami mual, muntah, dan ikterus setelah menerima terapi FDC. Pemeriksaan laboratorium menunjukkan peningkatan ALT dan AST, yang mengindikasikan DILI. Data dianalisis dengan pemantauan berkala enzim hati dan perubahan klinis. Hasil menunjukkan bahwa penghentian sementara OAT dan pemberian suplemen hepatoprotektor curcuma efektif dalam perbaikan fungsi hati. Penelitian ini menyarankan pemeriksaan rutin enzim hati pada pasien yang menerima terapi OAT untuk mencegah DILI. Alternatif terapi OAT dapat dipertimbangkan pada pasien dengan DILI berat.

Patient Satisfaction and Compliance in One-Day Care Chemotherapy: A Study at Dr. Moewardi Hospital Hadi, Cahyono; Isa, Muzakar; Sutrisna, Em; Pangestu, Mulyoto
JMMR (Jurnal Medicoeticolegal dan Manajemen Rumah Sakit) Vol. 14 No. 1 (2025): April 2025
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/jmmr.v14i1.531

The purpose of this study is to ascertain how satisfied and compliant cancer patients are with Dr. Moewardi Hospital's one-day care chemotherapy treatments, as well as how these factors relate to age, gender, education, and cancer type. Methods of research: This study used a descriptive method with a quantitative approach and correlational design. One hundred individuals who satisfied the inclusion and exclusion criteria made up the study's sample. A questionnaire was employed as the data collection method. According to the study's findings, 53% of chemotherapy patients were very satisfied, 37% were satisfied, and 10% were fairly satisfied when the five primary quality characteristics of tangibles, responsiveness, assurance, empathy, and reliability, were used to gauge their degree of satisfaction. 99% of cancer patients have complied with one-day care chemotherapy services. There is a noteworthy and favorable correlation between cancer patients' satisfaction and compliance with one-day care chemotherapy services. The main findings in this study indicate that most one-day care chemotherapy patients at Dr. Moewardi Hospital were very satisfied with the services provided, with a satisfaction level reaching 53%, and 99% of patients showed high compliance with the chemotherapy schedule. In addition, a significant and positive relationship was found between the level of satisfaction and patient compliance. Based on these findings, recommendations for further research are to explore non-demographic factors, such as socioeconomic status and family support, which may affect patient satisfaction and compliance, as well as to expand the study sample to obtain more comprehensive results.

Exploring the potential of Holothuria atra extract in modulating fasting triglyceride index and obesity: In silico, in vitro and in vivo studies Al’asyi, Assyu’ara; Ujianti, Irena; Nadika, Rara; Zahirah, Zahirah; Faizin, Bimo J.; Afifah, Devana A.; Farhan, Randhiva; Rahmadina, Salsabila P.; Lubis, Raisya ZN.; Ghaisani, Sakinah; Arkananta, Kiflan; Lakshmi, Bety S.; Pangestu, Mulyoto
Narra J Vol. 5 No. 3 (2025): December 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i3.2839

Obesity remains a major global health challenge and is strongly associated with metabolic disorders, particularly cardiovascular disease. This has fueled growing interest in natural interventions that regulate lipid metabolism as potential strategies to mitigate associated risks. Among these, Holothuria atra has emerged as a candidate due to its diverse bioactive compounds, though its mechanisms of action and therapeutic efficacy remain inadequately characterized. The aim of this study was to evaluate the combined effects of H. atra extract (HAE) and exercise on metabolic regulation, with the goal of determining whether their synergistic use enhances obesity management by targeting multiple metabolic pathways. Specimens of H. atra were collected from Tablolong Beach, Indonesia, and ethanol extracts were prepared. An in-silico analysis was performed to assess drug-likeness, quantitative structure-activity relationship (QSAR) properties, and network pharmacology. In vitro test using human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) underwent adipogenic differentiation with or without HAE treatment. This study used male Sprague-Dawley rats that were fed either a control or high-fat diet and further subdivided into groups receiving extract supplementation, swimming exercise, or a combination of both for six weeks. Liquid chromatography-mass spectrometry (LC-MS) analysis identified 6-gingerol and sarcostin as principal bioactive compounds, both of which fulfilled drug-likeness criteria. In silico analyses implicated peroxisome proliferator-activated receptor gamma (PPAR-γ) signaling as a major molecular target. In vitro tests found that HAE attenuated adipogenic differentiation of hUC-MSCs. Both HAE and exercise significantly reduced triglycerides, glucose, and the triglyceride–glucose index in rats fed a high-fat diet. The combination of HAE and exercise produced the greatest improvements, with significant reductions in glucose (p<0.01) , triglycerides (p<0.001), and the triglyceride–glucose index (p<0.001) compared to the high-fat diet control group. Collectively, these findings suggest that HAE, particularly when combined with regular exercise, improves lipid metabolism and may serve as a promising complementary strategy for obesity management.

Title

Found 8 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 8 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 8 Documents
Search