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All Journal HAYATI Journal of Biosciences Bioeksperimen: Jurnal Penelitian Biologi Jurnal Ilmu Pangan dan Hasil Pertanian Jurnal Surya Masyarakat Advance Sustainable Science, Engineering and Technology (ASSET) Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Dyah Ayu Widyastuti
Program Studi Biologi Fakultas Biologi UGM Yogyakarta
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Materials Science & Nanotechnology Public Health Other

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Journal : Advance Sustainable Science, Engineering and Technology (ASSET)

 1 
Activity of Moringa oleifera seed ethanolic extract against E. coli Nurafifah, Diah Ayu; Widyastuti, Dyah Ayu; Minarti, Ipah Budi
Advance Sustainable Science, Engineering and Technology Vol 3, No 2 (2021): May-October
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/asset.v3i2.9603

Abstract

Bacterial contamination in our environment is worrying, mainly contamination at drinking waters, vegetables, foods, soil which close to our daily activity. The main contamination in environment is caused by E. coli which simply found easily surround us. So, it leads to bigger problem if not immediately solve. One of possible yet safe compounds to overcome this problem is the use of natural product such as M. oleifera seeds as antibacterial agents. This study want to find out the ability of M. oleifera seed ethanolic extract as antibacterial agent against E. coli. Ethanolic extract of M. oleifera seeds are concentrated into 25%, 50%, and 75%, then treated to E. coli culture under laboratory condition. The inhibitory zone diameter which formed after 24 hours incubation was measured and compared to control with no extract treatment. The result of this study showed that there is inhibitory zone formed in three groups of treatmen (T1-T3), but there is no inhibitory zone formed at control group. The 75% ethanolic extract of M. oleifera seeds (T3) has the wides inhibitory zone diameter among four groups, followed by the 50% extract (T2) and 25% extract (T1) with diameter 15,03 ± 0,55 mm; 11,00 ± 1,32 mm; 7,03 ± 0,90 mm, respectively. All inhibitory zone diameter among groups in this research statistically different with strong inhibitory status at T2 as well as T3, and moderate inhibitory status at T1.
Comparison of Antioxidant Activity of Ethanolic, Methanolic, n-Hexan, and Aqueous Extract of Parkia speciosa Peel based on Half -Maximal Inhibitory Concentration Through Free Radical Inhibition Nurdyansyah, Fafa; Widyastuti, Dyah Ayu
Advance Sustainable Science, Engineering and Technology Vol 2, No 2 (2020): May-October
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/asset.v2i2.7129

Abstract

The objectives of this study was to determine the half maximum inhibitory concentration (IC50) from four types of Parkia speciosa peel extracts (ethanol, methanol, n-hexane, and aqueous)  through DPPH free radical inhibition. First Parkia’s peel extract made by drying the Parkia’s peel that has been sorted, then crushed and mashed with a blender. Parkia’s powder then macerated for 3 replication using each type of solvent and then solvent evaporation was carried out using a rotary vacuum evaporator. The evaporated extract produced then tested for antioxidant activity using the IC50 method and phytochemical screening was performed to analyze the potential content of functional compounds. The results showed that all types of solvents dissolve alkaloid compounds (except water extract), flavonoids, saponins, tannins, and phenols. IC50 values produced from the four types of petai bark extract using methanol, ethanol, water, and n-hexane solvents sequentially were 76.92; 111; 136; and 201 ppm. Methanol extract had the lowest IC50 value of 76.92 ppm which resulted that the methanol extract of petai skin had a strong (active) antioxidant strength compared to others.Keywords: Antioxidant, Exctract, Parkia’s peel, IC50
Co-Authors Andita Ayu Mandasari, Andita Ayu Dewi, Endah Rita Sulistyo fafa nurdyansyah, fafa Firdausi, Chilyatul Herawati, Dheasy Ipah Budi Minarti Kurnia, Muhammad Hardi Lussana Rossita Dewi Luthfiyah, Firya Maryam, Siti Ayu Maulana, Muhammad Iqbal Nurafifah, Diah Ayu Praptining Rahayu