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Fermentasi Nira Nipah Skala Pilot Menjadi Bioetanol Menggunakan Saccharomyces Cerevisiae Dengan Penambahan Tween 80 Dan Ergosterol Rafly Rafly; Chairul Chairul; Silvia Reni Yenti
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 3, No 1 (2016): Wisuda Februari Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Ethanol consumption of the world for a variety of uses has increased very significantly in recent years. Therefore it is necessary to alternate sources of raw materials to manufacture bioethanol and bioethanol production can be increased. Nypa sap is one of potential materials to be processed into bioethanol. This research was conducted the pilot scale fermentation of nypa sap, using Tween 80 and ergosterol as suplement to obtain high concentration of bioethanol as well as prevent osmosis in cells. This work is aimed to study of bioethanol pilot scale production from nypa sap, to determine effect of fermentation time to bioethanol and to obtain maximum conditions on the process. And to determine effect of Tween 80 and ergosterol. Fermentation was conducted in 70 L fermentor having variations of fermentation time such as 24; 36; 48; 60; 72; 84 and 96 hours respectively, variations addition of Tween 80 of 250; 375; and 500 ml respectively, and ergosterol of 25; 37,5; and 50 gr. Stirring speed of 200 rpm and temperature of fermentation at room temperature (25 - 300C). Ethanol concentration was analyzed by using Gas Chromatography. Tween 80 and ergosterol effect on the activity of Saccharomyces cerevisiae in converting nypa sap into bioethanol. The process of fermentation maximum conditions indicated in the addition of Tween 80 [375 ml] and ergosterol [37,5 g] at the time of 84 hours having the initial sugar concentration of 258,641 mg/ml. Concentration bioethanol obtained in this condition at 16,602% (v/v) or 131.040 mg/ml.Keywords: Bioethanol, Ergosterol, Nypa sap, saccharomyces cerevisiae, Tween 80
Uji In Vitro Hidroksiapatit Berpori Yang Dilapisi Kitosan Menggunakan Larutan SBF (Simulated Body Fluid) Dengan Variasi Penambahan Kitosan Toni Ardi; Ahmad Fadli; Silvia Reni Yenti
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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One of biological method for hydroxyapatite test is in vitro test, which is testing the material outside the system or not directly on living things. Some ways include using cell culture media, test tubes or SBF (Simulated Body Fluid) solutions. This research aims to determine the effect of variations of chitosan on the results of in vitro test of porous HA coated with chitosan. This research was carried out by synthesis CaO from cockle shells followed by synthesis HA from a mixture of CaO and NH4H2PO4 using low-temperature hydrothermal method. Porous HA is made by mixing HA, potato starch and distilled water using the starch consolidation method. A coating solution is prepared by mixing 0.75%w/v and 1.50%w/v into 2%v/v 100 ml acetic acid. The porous HA is then dipped into the solution for 60 minutes then dried at room temperature for 24 hours. The sample was then immersed in a SBF (Simulated Body Fluid) solution for 28 days. Samples were then analyzed using XRD and SEM analyzes. Apatite layers were formed on the entire surface of the samples based on SEM analysis. Samples have degree of crystallinity of 86.581%-88.067% with crystal diameter of 41.220-42.340 nm. The use of more chitosan as coating reduced degree of crystallinity and crystal diameter. Keywords: chitosan, hydroxyapatite, in vitro test, SBF (Simulated Body Fluid)
Pembuatan Bioetanol Dari Nira Nipah Menggunakan Bakteri Zymomonas Mobilis Dengan Variasi Pemekatan Medium Dan Waktu Fermentasi Wahyu Mey Riswanto; Silvia Reni Yenti; Chairul Chairul
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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The existence of Nypa palm is abundant in Riau province. Nypa can potentially supply biofuels because Its sap has a sugar content of 15-20% which can be converted into bioethanol. Bioethanol is ethanol produced from raw materials containing starch, sugar and cellulose through a process of fermentation and distillation that can be used as an alternative fuel which is environmentally friendly and renewable. To be Able to produce bioethanol from nypa sap in a laboratory scale, it is necessary to study the manufacture of bioethanol from nypa sap through fermentation by using Zymomonas mobilis fermentation medium volume of 2 liters. The objective of the research is to convert the sugar in the nypa sap through fermentation into bioethanol and to observe the effect of concentration medium of the juice in the fermentation and fermentation time on bioethanol production using Zymomonas mobilis. Through the process of fermentation using Zymomonas mobilis, glucose is converted into ethanol and carbon dioxide. Preparation starter was made with yeast inoculum process Zymomonas mobilis at 10% of the fermentation medium, therefore it can be adaptable and ready for fermentation. Fermentation takes place in batches with a volume of 2 liters of fermentation medium, concentration variations sap by evaporation at 10; 20; 30% (v/v) as well as variations in the fermentation time of 12; 24; 36; 48; 60; 72 and 84 hours. Temperature fermentation at room temperatur is 25 – 30oC. Ethanol concentration was Analyzed by using Gas Chromatography. Optimum conditions of bioethanol production from Nypa Sap were shown having 30% concentrated medium variation, and fermentation hours 60th about 15,164 % (v/v) or 111,689 mg/ml.Keywords: bioethanol, concentrated, nypa Sap, zymomonas mobilis
Pemurnian Bioetanol Hasil Fermentasi Nira Nipah Menggunakan Proses Destilasi-Adsorpsi Menggunakan Adsorben Silica Gel Shintia Oktaviani; Chairul Chairul; Silvia Reni Yenti
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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The rapid rate of world population growth conduces demand and supply of fuel is not balance. So that, energy reserves has been depleted in large numbers. Especially fossil fuels which is a main energy. The government has compiled program and policy for development of bioethanol and biodiesel to resolve energy crisis in indonesia which targeted in 2025 can be approximately 15-20% fuels need to fulfill transportation sector and the national industry. This research aims to produce bioethanol fuel grade-adsorption using distillation methods, determine the effect of the ratio of silica gel: bioethanol and height of the adsorbent in the column for producing bioethanol fuel grade. The raw material is bioethanol from nipa sap fermentation results with 13% ethanol content. Ethanol content obtained not too high previously, so that should be took a process that can be increase the purity of bioethanol with distillation process-adsorption. This research divided into two stages. First stage is nipa sap bioethanol distiled up to 96% concentration and second stage is distillation-adsorption using silica gel adsorbent. Independent variables on this research is ratio of silica gel : bioethanol and height of adsorbent in the adsorption column. The most influence process to review bioethanol purification is distillation-adsorption silica gel with ratio: ethanol 1:2 and a height of adsorbent in the column is 3.5 cm with purity of 99,6% and had been categorized fuel grade bioethanol.Keywords: Silica gel, bioethanol, bioethanol fuel grade, nypa, distillation adsorption process
Adsorption Kinetics Study of Cr (III) Metals Using Activated Natural Zeolite Retno Khimantoro; Sunarno Sunarno; Silvia Reni Yenti
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 1, No 2 (2014): Wisuda Oktober Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Several methods have been developed to reduce and minimize the presence of copper heavy metals that pollute waters, such as by adsorption method, is one of the natural  zeolite material is widely used as an adsorbent. Natural zeolites have potential as adsorption heavy metals in waste water treatment due to adsorption capacity and high selectivity, long-lived, and the numbers are abundant in Indonesia. Research studies of metal adsorption kinetics of Cr  (III) with activated natural zeolite aims to determine a suitable kinetic model for metal adsorption kinetics of Cr  (III) with activated zeolite  adsorption evaluate the metal Cr  (III) and the effect of temperature on the adsorption rate constant. This study begins with the activation of natural zeolite in physics and chemistry and to be continued to Cr  (III) metal adsorption by activated natural zeolite with a variety of contact time and temperature. Results spectrophotometer adsorption measured using atomic absorption (AAS). From the research that has been done shows that a suitable model for the kinetics of adsorption of metal Cr  (III) with zeolite activated following the model of first  order with a value of k= 0,011  min-1 for a temperature variation of 300C, 400C, 500C. From the results obtained conclude that the higher the temperature will be faster the rate of adsorption and the amount of activation energy values obtained in the amount of 296,73 J/mole, which means that  the adsorption process takes place in physics. Keyword: adsorption kinetics, Cr (III) metal, natural zeolites, adsorption .
Isolasi Kitin Dari Limbah Cangkang Kepiting (Scylla SP) Dengan Variasi Pelarut Pada Proses Bleaching Frederika Mawarni Adilasari Nduru; Drastinawati Drastinawati; Silvia Reni Yenti
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Crab shell is a waste that reach 50-60% of total weight, with chitin content of 20-30%. Chitin is biopolymer commercially used in biochemistry, medicine, textile, agriculture and others. The main aim of this research is to compare the level of lightness and whiteness of chitin produced in the bleaching process with organic and inorganic solvents. The research was started by reducing the size of the crab shell into a powder. Chitin was isolated by deproteination process using 3.5% NaOH and demineralization process using HCl 1N, followed by bleaching process with organic solvents variation of methanol; ethanol; acetone; ethanol:acetone (1:1), inorganic solvents of NaOCl 4%; Ca(OCl)2 3%; H2O2 3%, and the combination of acetone fallowed by NaOCl 0.315%. The result obtained is chitin without bleaching process with a yield of 24.44%, water content of 4%, and ash content of 1.75%. The IR spectrum analyzed before and after bleaching process showed relativity similiar absorption and were not affected by the solvent used in bleaching process. The color of chitin was analyzed using colorimeter. The result is the use of acetone fallowed by NaOCl 0.315% yileding chitin with 100% lighteness, 99.95% whiteness and categorized as white.Keywords: bleaching, crab shell, chitin, lightness, and whiteness.
Pengaruh Kecepatan Rotor Dan Konsentrasi Katalis Pada Pembuatan Biodiesel Dari Cpo Dengan Reaksi Esterifikasi Menggunakan Sentrifugal Kontaktor Gery Azhari Putera; Idral Amri; Silvia Reni Yenti
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Fuel needed for diesel engines in Indonesia each year increased along with the number of industrial machinery and the amount of diesel vehicles engine. With limited oil reserves, it is necessary to look for alternative sources of energy. Centrifugal contactor technology is one of the alternative technology of biodiesel production is very likely to be developed. Therfore, we need a study of biodiesel production using palm oil feedstock and methanol as reagent and sulfuric acid as a catalyst by using the esterification reaction. And this study used centrifugal contractors as the main equipment, which is equipped with a heater, raw material tank, product tank and pumps. Fixed variables selected in this study is the reaction time of 120 minutes, the molar ratio of feed is 9:1, and 60 ° C operating temperature. While the manipulated variable is rotor speed (1200, 1800 and 2400 rpm), the concentration of sulfuric acid catalyst (5%, 10% and 15% v/v methanol). The results of the research that has been done is the characteristics of biodiesel produced in this study, the kinematic viscosity values from 2,41 to 2,51 mm2/s, density 858-863 kg/m3, and acid number 6,30 to 6,66 mg-KOH/g. The composition of the methyl ester obtained is 0,33% behenic metyl ester, 48,87% palmitic methyl ester and 50,80% oleic methyl ester. The best conversion is 56,03% at the optimum operating conditions with a catalyst concentration of sulfuric acid 10% v/v methanol and the rotor speed of 2400 rpm. Correlation of rotor speed on reaction rate constant is k = 0,0002Re(0,1928) . And the relationship of the catalyst concentration the reaction rate constant is k = 0,0055[Q] 0,089.          Keywords: CPO, Centrifugal Contactors, Biodiesel, Esterification Reaction
Fermentasi Nira Nipah Skala 50 Liter Menjadi Bioetanol Menggunakan Saccharomyces Cerevisiae Ade Sri Umaiyah; Chairul Chairul; Silvia Reni Yenti
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 1, No 1 (2014): Wisuda Februari Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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One of the natural resources that can be utilized alternative into bioethanol is palm sap. Preparation stater done by culturing medium Saccharomyces cereviseae as developer on 10% yeast fermentation medium thus able to adapt and be ready to do the fermentation. This study aims to make bioethanol from palm sap 50-liter scale with variations in pH 4,5; 5 and 5,5 and fermentation time 24, 36, 48, 60 and 72 hours.. Batch fermentation process takes place with a stirring speed of 200 rpm and temperature of fermentation at room temperature (25-300C). The optimum fermentation conditions indicated on the initial conditions of pH 4,5 and fermentation time of 48 hours the acquisition of or 9% .  Keywords: Bioethanol, palm sap, fermentation, Saccharomyces cerevisiae
Esterifikasi Gliserol Sebagai Produk Samping Biodiesel Menjadi Triacetin Dengan Menggunakan Katalis Zeolit Alam Pada Variasi Suhu Reaksi Dan Konsentrasi Katalis Dedi Meier Silaban; Zuchra Helwani; Silvia Reni Yenti
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Glycerol is by-product of biodiesel. Production of biodiesel will produce glycerol about 10% of total biodiesel. The excess glycerol able to treatment to a more expensive product, such as triacetin. Triacetin is a triester from glycerol and acetic acid. It is used as good additive and also soluble in biodiesel. Triacetin was produced by esterification of glycerol and acetic acid. The aim of this research was to know the influence of the concentration of catalyst and esterification temperature reaction toward glycerol conversion. Esterification of glycerol was done by activated natural zeolite catalyst. The molar ratio of glycerol and acetic acid was 1:7. The temperature that used was 90oC, 100oC, and 110oC, than the concentration of zeolite catalyst was 2%, 4%, and 6% of acetic acid mass. The highest glycerol conversion was 85,21% that reached in reaction temperature 110oC and catalyst concentration 4% of acetic acid mass. The temperature and catalyst concentration of esterification was directly proportional of the glycerol conversion.Key words: Biodiesel, esterification, glycerol, triacetin, zeolite.
Sintesis Zeolit 4A Dari Abu Limbah Sawit Dengan Variasi Ukuran Partikel Abu Sawit Dan Variasi Volume Natrium Silikat Dengan Natrium Aluminat Lanie Faradina; Fajril Akbar; Silvia Reni Yenti
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 3, No 2 (2016): Wisuda Oktober Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Zeolite defines as hydrated aluminosilicate minerals which have 3 dimension framework structures formed of silica and alumina tetrahedral by sharing all the oxygen atoms and content with balanced metal load ions of alkaline or alkaline earth metals. Zeolite 4A is synthetic zeolite which have Na+ cation in balancing up the negative charge. Purpose of this research to get advantage from palm ash as a source of silica as feed for zeolite 4A and studied the influence of the palm ash size and ratio of reactan volume. The variation size of palm ash is 60,100, 200 mesh and unsifted ash. Sythesis of zeolite 4A doing by mixing sodium silicate and sodium aluminate with variaton of volume at 55:45 and 60:40 ml/ml and rate of agitation at 200 rpm. Then the gel formed will be heated in the oven at 80oC for 8 hours and washed until pH neutral. After washing process, sample will be dried at 120oC for 3 hours. Sythesis product analyzed with IR Spectroscopy and Xray Difraction. The best result was got in variation size 100 mesh and volume at 55:45 ml/ml.Keyword: Palm Ash, Xray Difraction, IR Spectroscopy, Zeolite 4A