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All Journal VALENSI AL KAUNIYAH Buletin Plasma Nutfah Livestock and Animal Research Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUSCIENCE Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Journal of Aquaculture and Fish Health Journal of Fish Nutrition
Etyn Yunita
Program Studi Biologi, Fakultas Sains Dan Teknologi, UIN Syarif Hidayatullah Jakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Veterinary

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FERMENTATION OF KEPOK BANANA PEEL-CORN HOMINY MIXED SUBSTRATE FOR DIETARY INCLUSION IN BROILER RATION Anwar, Fadilla; Sriherwanto, Catur; Yunita, Etyn; Suja’i, Imam
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 1 (2016): June 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.093 KB) | DOI: 10.29122/jbbi.v3i1.54

Abstract

To reduce broiler production cost, a study was carried out on utilisation of alternative, less costly feed components, namely kepok banana peel and corn hominy which were mixed and fermented using the fungus Rhizopus oryzae. The fermented substrate was added into commercial feed in order to determine its influence on the poultry’s production performance. This study employed a completely randomized design (CRD) with four treatments: one using 100% pure commercial feed, and the other three commercial feed mixed with the fermented feed component at the levels of 5%, 10% and 15%. All treatments were repeated four times during 25 days growth period. The results showed that supplementation of the fermented ingredient in the broiler ration at the levels of 5%, 10%, or 15% had no significant effect (P>0,05) on the feed consumption, body weight gain, feed conversion ratio, and the final body weight of the animals. Although mixing the fermented feed ingredient up to 15% in the commercial feed promoted the growth of the broilers, the results were not yet comparable to pure commercial feed.Keywords: Corn hominy, broiler, fermentation, kepok banana peel, Rhizopus oryzae AbstrakUntuk mengurangi biaya produksi ayam pedaging, dilakukan pengkajian penggunaan bahan penyusun pakan alternatif yang lebih murah, yaitu kulit pisang kepok dan ampok jagung yang dicampur dan difermentasi menggunakan jamur Rhizopus oryzae. Hasil fermentasi tersebut lalu ditambahkan pada pakan komersial ayam pedaging dengan tujuan mengetahui pengaruhnya terhadap penampilan produksi ayam pedaging. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan empat perlakuan, yaitu satu perlakuan 100% pakan komersial murni, dan tiga perlakuan pakan komersial yang dicampur bahan pakan hasil fermentasi tersebut dengan kadar 5%, 10%, dan 15%. Seluruh perlakukan diulang empat kali selama 25 hari masa pemeliharaan. Hasil penelitian menunjukkan bahwa penambahan bahan pakan terfermentasi ke dalam ransum ayam pedaging pada kadar 5%, 10%, atau 15% tidak memberikan pengaruh nyata (P>0,05) terhadap konsumsi pakan, pertambahan bobot badan, nilai koversi pakan, dan bobot badan akhir hewan. Meskipun penambahan bahan pakan fermentasi tersebut hingga 15% dalam pakan komersial mendukung pertumbuhan ayam pedaging, namun capaian pertumbuhannya belumlah sebaik pakan komersial.Kata kunci: Ampok jagung, ayam pedaging, fermentasi, kulit pisang kepok, Rhizopus oryzae
PERUBAHAN KANDUNGAN ASAM FITAT DAN ASAM AMINO ESENSIAL BAHAN-BAHAN ORGANIK PAKAN YANG DIFERMENTASI RAGI TEMPE Muniroh, Arina; Suja'i, Imam; Wibowo, Aji; Saputra, Henry Kasman Hadi; Yunita, Etyn; Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 1 (2021): June 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (996.834 KB) | DOI: 10.29122/jbbi.v8i1.4743

Abstract

Changes in the Contents of Phytic Acid and Essential Amino Acids of Animal Feed Organic Materials Fermented by Tempe Starter The contents of antinutrients and nutrients are important in the selection of feed ingredients, especially for monogastric animals. The aim of this study is to determine the change in the contents of antinutrient phytic acid and nutrient essential amino acid in selected organic materials. Nine organic ingredients of agro-industrial by-products, namely rice bran, coffee skin, cassava bagasse, corn, coconut dreg, soy bean meal, pollard, corn gluten feed (CGF), and copra meal were fermented in solid state using tempeh starter for 48 hours at 30°C. Dense mycelia overgrowing evenly on the top, bottom, and cross-sliced surfaces was observed on rice bran, maize and copra meal substrates. These 3 selected organic materials were then analysed for the content of phytic acid and essential amino acids. The results showed that the lowest decrease (75.80%) in phytic acid content occurred in maize, namely from 18.49 ± 0.41 mg g-1 (before fermentation) to 4.48 ± 0.19 mg g-1 (after fermentation). The highest increase (59%) of total essential amino acids occurred in copra meal, namely from 38,991.89 ± 447.12 mg kg-1 (before fermentation) to 61,816.56 ± 894.24 mg kg-1 (after fermentation). Kandungan antinutrisi dan nutrisi merupakan hal penting dalam pemilihan bahan pakan, terutama untuk hewan monogastrik. Penelitian ini bertujuan mengetahui perubahan kandungan antinutrisi asam fitat dan nutrisi asam amino esensial pada bahan organik tertentu yang terpilih. Sembilan bahan organik yang merupakan hasil samping agroindustri, yakni dedak padi, kulit kopi, onggok, jagung, ampas kelapa, bungkil kedelai (soy bean meal), dedak gandum (pollard), produk samping jagung corn gluten feed (CGF), dan bungkil kopra difermentasi padat menggunakan ragi tempe selama 48 jam pada suhu 30°C. Miselium yang tumbuh subur, padat, dan merata pada permukaan atas, bawah, dan irisan melintang teramati pada dedak padi, jagung, dan bungkil kopra. Tiga bahan organik terpilih ini kemudian dianalisa kandungan asam fitat dan asam amino esensialnya. Hasil menunjukkan bahwa penurunan terbesar (75,80%) kandungan asam fitat terjadi pada jagung, yakni dari 18,49 ± 0,41 mg g-1 (sebelum fermentasi) menjadi 4,48 ± 0,19 mg g-1 (setelah fermentasi). Peningkatan tertinggi (59%) asam amino esensial total terjadi pada bungkil kopra, yakni dari 38.991,89 ± 447,12 mg kg-1 (sebelum fermentasi) menjadi 61.816,56 ± 894,24 mg kg-1 (setelah fermentasi).
FERMENTATION OF KEPOK BANANA PEEL-CORN HOMINY MIXED SUBSTRATE FOR DIETARY INCLUSION IN BROILER RATION Fadilla Anwar; Catur Sriherwanto; Etyn Yunita; Imam Suja’i
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 1 (2016): June 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.093 KB) | DOI: 10.29122/jbbi.v3i1.54

Abstract

To reduce broiler production cost, a study was carried out on utilisation of alternative, less costly feed components, namely kepok banana peel and corn hominy which were mixed and fermented using the fungus Rhizopus oryzae. The fermented substrate was added into commercial feed in order to determine its influence on the poultry’s production performance. This study employed a completely randomized design (CRD) with four treatments: one using 100% pure commercial feed, and the other three commercial feed mixed with the fermented feed component at the levels of 5%, 10% and 15%. All treatments were repeated four times during 25 days growth period. The results showed that supplementation of the fermented ingredient in the broiler ration at the levels of 5%, 10%, or 15% had no significant effect (P>0,05) on the feed consumption, body weight gain, feed conversion ratio, and the final body weight of the animals. Although mixing the fermented feed ingredient up to 15% in the commercial feed promoted the growth of the broilers, the results were not yet comparable to pure commercial feed.Keywords: Corn hominy, broiler, fermentation, kepok banana peel, Rhizopus oryzae AbstrakUntuk mengurangi biaya produksi ayam pedaging, dilakukan pengkajian penggunaan bahan penyusun pakan alternatif yang lebih murah, yaitu kulit pisang kepok dan ampok jagung yang dicampur dan difermentasi menggunakan jamur Rhizopus oryzae. Hasil fermentasi tersebut lalu ditambahkan pada pakan komersial ayam pedaging dengan tujuan mengetahui pengaruhnya terhadap penampilan produksi ayam pedaging. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan empat perlakuan, yaitu satu perlakuan 100% pakan komersial murni, dan tiga perlakuan pakan komersial yang dicampur bahan pakan hasil fermentasi tersebut dengan kadar 5%, 10%, dan 15%. Seluruh perlakukan diulang empat kali selama 25 hari masa pemeliharaan. Hasil penelitian menunjukkan bahwa penambahan bahan pakan terfermentasi ke dalam ransum ayam pedaging pada kadar 5%, 10%, atau 15% tidak memberikan pengaruh nyata (P>0,05) terhadap konsumsi pakan, pertambahan bobot badan, nilai koversi pakan, dan bobot badan akhir hewan. Meskipun penambahan bahan pakan fermentasi tersebut hingga 15% dalam pakan komersial mendukung pertumbuhan ayam pedaging, namun capaian pertumbuhannya belumlah sebaik pakan komersial.Kata kunci: Ampok jagung, ayam pedaging, fermentasi, kulit pisang kepok, Rhizopus oryzae
GROWTH OF CARP (Cyprinus carpio L.) FED WITH RICE BRAN-COCONUT BAGASSE MIXED SUBSTRATE FERMENTED USING Rhizopus oryzae Robby Dzul Umam; Catur Sriherwanto; Etyn Yunita; Imam Suja’i
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 2 (2015): December 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (934.308 KB) | DOI: 10.29122/jbbi.v2i2.513

Abstract

Dedak padi dan ampas kelapa dicampur dengan perbandingan tertentu dan kemudian difermentasi menggunakan Rhizopus oryzae untuk pakan ikan. Uji pemberian pakan lalu dilakukan untuk mengetahui pengaruh pakan terhadap pertumbuhan ikan mas (Cyprinus carpio L.). Dalam penelitian ini digunakan 5 perlakuan: satu perlakuan pakan tanpa fermentasi (pakan komersial 100%), dan empat perlakuan pakan fermentasi substrat campuran bekatul dan ampas kelapa dengan empat perbandingan yang berbeda, yakni 75%:25%, 50%:50%, 25%:75%, dan 0%:100%. Hasil penelitian menunjukkan bahwa pemberian pakan komersial 100% (protein sejati 15,25% dan serat kasar 6,27%) memperlihatkan hasil terbaik terhadap pertumbuhan ikan mas dengan pertambahan bobot badan 2,56 g dan rasio konversi pakan 1,95. Sementara itu pemberian pakan fermentasi (protein sejati berkisar 4,89-9,97% dan serat kasar 22,87-25,70%) hanya menghasilkan pertambahan bobot badan ikan pada kisaran 0,47-0,64 g dengan rasio konversi pakan 2,50-2,64. Dengan demikian pakan fermentasi tersebut mampu mendorong pertumbuhan ikan mas meskipun masih kurang optimal dibandingkan dengan pakan komersial.Kata Kunci: Rhizopus oryzae, Cyprinus carpio, rice bran, coconut bagasse, fermentation  ABSTRACTRice bran and coconut bagasse were mixed and then fermented using Rhizopus oryzae for preparing aquafeed. Subsequent feeding test was carried out to determine the effect on the growth of carps (Cyprinus carpio L). Five feeding treatments were employed, one unfermented feed (commercial feed 100%), and the other four feeds produced by fermentation using substrate mixture of rice bran and coconut pulp in four different ratios, namely 75%:25%, 50%:50%, 25%:75%, and 0%:100%. The results showed that feeding 100% commercial feed (true protein 15.25% and crude fibre 6.27%) showed the best results on the fish growth with body weight gain of 2.56 g and feed conversion ratio of 1.95. Meanwhile, feeding fermented feeds (true protein 4.89-9.97% and crude fiber 22.87-25.70%) only resulted in body weight gain in the range of 0.47 to 0.64 g with feed conversion ratio of 2.50 to 2.64. Thus, the fermented feeds promoted growth in tested carps albeit less optimally than commercial feed.Keywords: Rhizopus oryzae, Cyprinus carpio, dedak, ampas kelapa, fermentasi
GROWTH OF NILE TILAPIA (Oreochormis niloticus) FRY FED WITH COCONUT TESTA-CASSAVA BAGASSE MIXED SUBSTRATE FERMENTED USING Rhizopus oryzae Yudha Wali Pradana; Catur Sriherwanto; Etyn Yunita; Imam Suja’i
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 4 No. 1 (2017): June 2017
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (874.114 KB) | DOI: 10.29122/jbbi.v4i1.1799

Abstract

Utilization of agroindustrial byproduct as cheap raw materials for aquafeed was hampered by its poor nutritional value as well as high antinutrition content which could be overcome through fermentation. Coconut testa (CT) and cassava bagasse (CB) were mixed, and fermented using Rhizopus oryzae for preparing aquafeed. Subsequent feeding test was carried out on tilapias (Oreochormis niloticus L.) using 5 feeding treatments: one unfermented feed (commercial feed 100%), and the other four feeds produced by fermentation using substrate mixture of CT and CB in 4 different ratios, namely 100%:0%, 75%:25%, 50%:50%, and 25%:75%. Feeding 100% commercial feed (true protein 22.64% and crude fibre 14.67%) showed the best results on the fish growth with body weight gain of 3.96 g and feed conversion ratio of 8.63. Meanwhile, feeding fermented feeds (true protein 7.96-20.27% and crude fiber 14.14-18.47%) resulted in body weight gain in the range of 2.22 to 2.75 g with feed conversion ratio of 10.89 to 13.62. Thus, the fermented feeds promoted growth in tested tilapias albeit less optimally than commercial feed did.Keywords: Rhizopus oryzae, Oreochromis niloticus, coconut testa, cassava bagasse, fermentation ABSTRAKPenggunaan hasil samping agroindustri sebagai bahan pakan murah ikan terkendala rendahnya nutrisi dan tingginya antinutrisi yang dapat diatasi melalui fermentasi. Dalam penelitian ini, kulit daging buah kelapa (KK) dan onggok singkong (OS) dicampur dengan perbandingan tertentu, lalu difermentasi menggunakan Rhizopus oryzae untuk pakan ikan. Uji pemberian pakan dilakukan terhadap ikan nila (Oreochormis niloticus L.) dengan 5 perlakuan: satu perlakuan pakan tanpa fermentasi (pakan komersial 100%), dan empat perlakuan pakan fermentasi substrat campuran KK dan OS dengan 4 perbandingan yang berbeda, yakni 100%:0%, 75%:25%, 50%:50%, dan 25%:75%. Pemberian pakan komersial 100% (protein sejati 22,64% dan serat kasar 14,67%) memperlihatkan hasil terbaik pada pertumbuhan ikan nila dengan pertambahan bobot badan 3,96 g dan rasio konversi pakan 8,63. Sebaliknya, pemberian pakan fermentasi (protein sejati berkisar 7,96-20,27% dan serat kasar 14,14-18,47%) menghasilkan pertambahan bobot badan ikan pada kisaran 2,22-2,75 g dengan rasio konversi pakan 10,89-13,62. Dengan demikian pakan fermentasi tersebut mendorong pertumbuhan ikan nila namun kurang optimal dibandingkan pakan komersial.Kata Kunci: Rhizopus oryzae, Oreochromis niloticus, kulit daging buah kelapa, onggok, fermentasi
KARAKTERISTIK FISIK PAKAN IKAN APUNG NON-EKSTRUSI YANG DIBUAT MELALUI FERMENTASI Rhizopus oryzae Asep Badru Zaman; Catur Sriherwanto; Etyn Yunita; Imam Suja'i
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1101.663 KB) | DOI: 10.29122/jbbi.v5i1.2793

Abstract

Physical Characteristics of Non-Extruded Floating Fish Feed Produced through Rhizopus oryzae Fermentation ABSTRACTTo reduce the feed production cost of floating fish feed, an innovative method which is cheaper, easier, and simpler compared to the use of extruder machine is needed to provide buoyancy to fish feed. This research was conducted to determine the physical characteristics of floating fish feed prepared through fermention using Rhizopus oryzae on the mixed substrate consisting of commercial sinking fish feed, cassava bagasse, and Lemna minor. The resulting fermented feed was subsequently compared with commercial sinking fish feed in terms of its water stability, absorption, and floatability. The water stability curves of both feed types showed a similar pattern for 20 hours. Both decreased dramatically in the first 0.5 hours, from 100% to 81% for the fermented feed, and to 83% for the commercial feed. The shape of a 40-minute water absorption curve for both feed types was very similar. The fermented feed absorbed water nearly 4 times its initial dry weight, while commercial feed up to about twice as much as its initial dry weight. For three hours, both fermented and commercial floating pellets stayed afloat 100% without sinking. Thus, fermentation using R. oryzae deserves further development as an alternative way to provide buoyancy to fish feed.Keywords: floatability, floating feed, sinking feed, water absorption, water stability ABSTRAKUntuk menekan biaya produksi pakan ikan apung, diperlukan inovasi pengapungan pakan ikan yang lebih murah, mudah, dan sederhana dibandingkan dengan penggunaan mesin ekstruder. Penelitian ini dilakukan untuk mengetahui karakteristik fisik pakan apung hasil fermentasi menggunakan kapang Rhizopus oryzae pada substrat campuran pakan tenggelam komersial, onggok, dan Lemna minor. Pakan fermentasi ini lalu dibandingkan dengan pakan apung komersial dalam hal stabilitas dalam air, absorbsi air, dan daya apung. Stabilitas dalam air kedua jenis pakan memiliki pola yang mirip selama 20 jam. Stabilitas keduanya menurun drastis pada 0,5 jam pertama, dari 100% ke 81% untuk pakan apung fermentasi, dan ke 83% untuk pakan apung komersial. Bentuk kurva absorpsi air selama 40 menit untuk kedua jenis pakan sangatlah mirip. Pakan apung fermentasi menyerap air hampir 4 kali bobot kering awalnya, sedangkan pakan apung komersial hingga sekitar 2 kali bobot kering awalnya. Selama tiga jam, baik pakan apung fermentasi maupun komersial mengapung 100% tanpa tenggelam. Jadi, fermentasi menggunakan R. oryzae layak dikembangkan lebih lanjut sebagai cara pengapungan alternatif pakan ikan.Kata Kunci: daya apung, daya serap air, stabilitas dalam air, pakan apung, pakan tenggelam
Diversity of Quantitative and Qualitative Characters of Rice Grain from Riau Province, Indonesia Nurul Hidayatun; Devi Ramadyanti; Dodin Koswanuddin; Etyn Yunita
Buletin Plasma Nutfah Vol 27, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v27n2.2021.p125-132

Abstract

Rice is grown in almost all regions in Indonesia, including Riau Province, as a staple food. Although rice is the most widely grown food crop in comparison to other crops, rice production from this region has not been able to meet the needs of the Riau population. To meet the needs of rice, the people of Riau still have to bring it from other regions. Riau Province has a large collection of diverse rice germplasm for food self-sufficiency. Several rice accessions from the area have been collected and conserved in the Genebank of the Indonesian Agency for Agricultural Research and Development (IAARD), located at Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD). This study was intended to assess the diversity of rice from Riau Province based on the grain characteristics. A total of 25 rice accessions from Riau which were conserved in the IAARD-ICABIOGRAD Genebank were examined. Grain length, shape, color, and other grain-related characters were observed following the international standard descriptors for rice by IRRI and IBPGR. While the thousand-grain weight was measured using the standardized operational procedure from ISTA. The grain diversity was analyzed using the NTSYS-pc software version 2.2. The average of length, width, and thickness of the unhulled paddy was measured at 7.9, 3.0, and 1.9 mm, respectively. The weight of 1,000 grains ranged from 20.37 to 25.67 with an average of 23.02 g. Most of the accessions had a brown furrow or spot on straw background lemma-palea, with a straw apiculus a medium-sized sterile lemma, and were awnless. The majority of the rice grain was medium size, measuring 5.24 mm in length and 2.24 mm in width. White grain makes up the majority of the accessions. Accessions from Riau can be divided into five groups based on the seven characteristics of rice grains. Those groupings were unrelated to the district from which the accessions were obtained, illustrating the beyond boundaries of germplasm distribution and transfer of germplasm within this district.
Kandungan Pb Pada Daun Angsana (Pterocarpus indicus) dan Rumput Gajah Mini (Axonopus.Sp)Di Jalan Protokol Kota Tangerang Siti Nihayatul Inayah; Thamzil Las; Etyn Yunita
Jurnal Kimia Valensi Jurnal Valensi Volume 2, No.1, November 2010
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.29 KB) | DOI: 10.15408/jkv.v2i1.233

Abstract

Telah dilakukan penelitian untuk mengetahui akumulasi Pb pada daun Angsana (Pterocarpus indicus) dan rumput Gajah Mini (Axonopus.Sp) yang terletak dibeberapa jalan protokol Kota Tangerang. Lokasi pengambilan sampel dilakukan di Sembilan lokasi utama Kota Tangerang dan satu di lokasi permukiman. Pengambilan sampel dilakukan pada bulan Maret dan April 2009. Sampel dianalisa menggunakan Spektroskopi Serapan Atom (SSA) melalui metode destruksi basah. Hasil yang didapat dari penelitian ini adalah kandungan Pb pada daun Angsana (2.04 – 7.30 μg/g pada bulan Maret 2009 ; 1.12 – 7.61 μg/g pada bulan April 2009) dan rumput Gajah Mini (2.12 – 12.38 μg/g pada bulan Maret 2009 ; 5.89 – 10.32 μg/g pada bulan April 2009). Secara umum dapat disimpulkan bahwa Pterocarpus indicus dan Axonopus.Sp mampu mengakumulasi Pb pada kisaran 1.12-12.38 μg/g. Kandungan Pb pada daun Angsana dan rumput Gajah Mini hasil penelitian tidak mencapai 1000 ppm (μg/g). Hal ini berarti kandungan Pb pada daun Angsana dan rumput Gajah Mini di Kota Tangerang belum melampaui ambang batas toksisitasnya terhadap tanaman.
Fitoplankton sebagai Bioindikator Saprobitas Perairan di Situ Bulakan Kota Tangerang Sinta Ramadhania Putri Maresi; Priyanti Priyanti; Etyn Yunita
Al-Kauniyah: Jurnal Biologi Vol 8, No 2 (2015): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (455.839 KB) | DOI: 10.15408/kauniyah.v8i2.2697

Abstract

Industry, homes activites, and aquaculture around situ or lake could contaminate and affect the water quality. This study aimed to determine the diversity of phytoplankton and water pollution level in Situ Bulakan Kota Tangerang based on saprobite index. The research was conducted on December 2014 until March 2015 at Situ Bulakan Kota Tangerang. Observations were made based on 4 points sampling with three repetitions for one day that could represented existing condition. Sampling sites in this study were at inlet flow, middle, outlet, and alleged utilized areas such as floating fish net. The result showed  26 species of phytoplankton from four classes, namely 9 species of Cyanophyceae, 4 species of Bacillariophyceae, 11 species of Chlorophyceae, and 2 species of Euglenophyceae with moderate diversity index (H' = 1.77─2.05). Monoraphidium sp. was a species dominated in Situ Bulakan Kota Tangerang which was found 215 individuals. So it can be said as saprobite bioindicator. Saprobite index in Situ Bulakan Kota Tangerang showed moderate to severe contaminated with α-mesosaprobic (0.14).
Pengaruh Pemberian Nannochloropsis oculata terhadap Kadar Total Kolesterol dan Berat Badan Tikus Sprague Dawley yang Mengalami Hypercholesterolemia Diini Fithriani; Etyn Yunita; Ajeng Kurniasari; Fauzan Martha
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 2 (2019): Desember 2019
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.419 KB) | DOI: 10.15578/jpbkp.v14i2.357

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian Nannochloropsis oculata dengan dosis bertingkat terhadap kadar kolesterol total tikus yang dibuat hypercholesterolemia dan pengaruhnya terhadap perubahan berat tikus. Penelitian ini bersifat eksperimental menggunakan hewan percobaan tikus Wistar (Sprague Dawley) jantan.  Sampel terdiri dari 25 ekor tikus  Sprague Dawley yang secara acak dibagi menjadi menjadi 5 kelompok. Kelompok A adalah kelompok kontrol negatif yang diberi pakan standar, kelompok B adalah kontrol positif yang diberi pakan hiperkolesterol, dan  kelompok  C, D dan  E sebagai kelompok perlakuan yang diberikan pakan hiperkolesterol dan pemberian N. oculata dengan dosis 150 mg/ kg bb (kelompok C), 300 mg/ kg bb (kelompok D), dan 600 mg/ kg bb (kelompok E).  Hasil penelitian menunjukkan bahwa N. oculata memberikan pengaruh positif terhadap penurunan total kolesterol. Semakin tinggi dosis mikroalga yang diberikan maka semakin tinggi penurunan total kolesterol dalam darah. Total penurunan kolesterol pada dosis 150 mg/kg bb adalah 21%, sedangkan pada dosis 300 mg/kg bb adalah 34% dan pada dosis 600 mg/kg bb adalah 42%. Pada kontrol positif mengalami  penurunan 12%, sedangkan kontrol negatif meningkat 3%.  Selain itu, diperoleh hasil bahwa terdapat interaksi periode waktu dan perlakuan terhadap kadar kolesterol. Hasil uji in vivo ini menunjukkan bahwa mikroalga N. oculata berpotensi untuk dikembangkan sebagai suplemen penurun kolesterol dengan dosis efektif 600 mg/kg bb. Pemberian N.oculata  pada dosis 150 mg-600 mg/kg bb selama 28 hari dapat menurunkan jumlah pakan yang dikonsumsi namun jumlahnya belum secara signifikan mempengaruhi berat badan tikus. The Effect of Nannochloropsis oculata on the Decrease in Total Cholesterol Levels and Body Weight  of Sprague Dawley Rats with Hypercholesterolimia AbstractThe aim of this research was to study the effect of Nannochloropsis oculata on the total cholesterol level of hypercholesterolemia rat and the changes of rat weight. This experimental study was conducted using male Wistar (Sprague Dawley) rat. The samples consisted of 25 Sprague Dawley rats that were randomly divided into 5 groups. Group A was a negative control group (standard fed), group B was a positive control (fed with hypercholesterolemia) and the C, D and E groups were the treatment groups given with hypercholesterol feed and N. oculata which was administered orally at a dose of 150 mg/kg bw (group C), 300 mg/kg wb (group D), and 600 mg/kg bw (group E). The results showed that microalgae N. oculata gave a positive effect on the total reduction of cholesterol. The higher the dose of the given microalgae the lower total cholesterol in the rat blood. The total decreased of cholesterol at dose of 150 mg/kg bw was 21%, while at dose of 300 mg/kg bw was 34%, at dose of 600 mg/kg bw was 42%, and in positive control was 12%. However, the negative control increased the cholesterol level at 3%. Statistically, the results showed that there was an interaction between the treatment and the day periode of treatment on the cholesterol blood level. This in vivo test showed that N. oculata is potential as a functional food for reducing cholesterol level with an effective dose of 600 mg/kg bw. Giving N. oculata at a dose of 150 mg-600 mg/kg bw for 28 days can reduce the amount of feed consumed but the amount has not significantly affected the body weight of rat.
Co-Authors Ajeng Kurniasari Aji Wibowo Aldri Frinaldi Asep Badru Zaman Azizah Mei Widyastuti Bimo Saputro Catur Sriherwanto Catur Sriherwanto Catur Sriherwanto Catur Sriherwanto Catur Sriherwanto Catur Sriherwanto Catur Sriherwanto Catur Sriherwanto, Catur Dasumiati Dasumiati Deska Ulul Muta’al Devi Ramadyanti Dewi Elfidasari Diini Fithriani Dodin Koswanuddin Fadilla Anwar Fadilla Anwar, Fadilla Fauzan Martha Firsoni Firsoni, Firsoni Hanny Hafiar Hardani, Shintia Nugrahini Wahyu Imam Suja'i Imam Suja'i Imam Suja'i Imam Suja'i Imam Suja’i, Imam Imam Suja’i Imam Suja’i Imam Suja’i Imam Suja’i Imam Suja’i Irawan Sugoro Irzal Irda Jatmiko, Eko Lili Sholichah Liza Nurohmah Mirjani Adila, Mirjani Muniroh, Arina Nadhilah Sabila Ghaisani Nina Meilisza Norma Sulistianingsih Nurul Hidayatun Priyanti Priyanti Rina Hirnawati Robby Dzul Umam Robby Dzul Umam, Robby Saputra, Henry Kasmanhadi Sinta Ramadhania Putri Maresi Siti Murniasih Siti Nihayatul Inayah Suja'i, Imam Suja’i, Imam Sujai, Imam Sukarman Sulistianingsih, Norma Thamzil Las Thamzil Laz, Thamzil Yudha Wali Pradana Yudha Wali Pradana, Yudha Wali Zaman, Asep Badru Zaman, Asep Badru