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KARAKTERISTIK MORFOLOGI DAN POLA PITA IZOSIM VARIETAS MANGGA (Mangifera sp.) DI KABUPATEN BANYUMAS , Sumarsono; Suparjana, Tata Brata; Purwati, Endang Sri
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Mangga (Mangifera indica) merupakan salah satu tanaman hortikultura yang memiliki nilai ekonomi tinggi. Untuk membedakan antara varietas mangga, penanda genetik yang digunakan karena mereka tidak dipengaruhi oleh faktor lingkungan. Salah satu penanda genetik yang sering digunakan adalah Isozim. Tujuan penelitian ini adalah untuk mengetahui pola bandeng dan variasi genetik beberapa varietas mangga dari Kabupaten Banyumas berdasarkan peroksidase isozim (PER), aspartate aminotransferase (AAT), esterase (EST), asam fosfatase (ACP). Penelitian ini dilakukan di Pabrik Laboratorium Biologi, Biologi Pusat Penelitian Ilmu, IPB, Bogor dari Juni sampai September 2012. Sampling daun dari tujuh varietas mangga dilakukan dengan purposive random sampling. Setelah eletrophoresis dan scoring, data kemudian dianalisis dengan Unweighted Pair-group Method with Arithmetic (UPGMA) under Numerical Taxonomy and Multivariate Sistem (NTSYS) version 2.20i. Hasil penelitian menunjukkan bahwa EST memiliki empat pola pita yang bermigrasi anodally. AAT memiliki lima pola banding dan ACP memiliki lima pola pita yang bermigrasi anodally. PER memiliki lima pola pita yang bermigrasi anodally dan katodally, MG4 (lokal dari indramayu mangga) tidak muncul. PER, AAT, ACP dan ACP menunjukkan pola yang berbeda dari dendogram. Kombinasi dari empat isozim menunjukkan bahwa variasi genetik antara MG4 (mangga lokal indramayu) dan MG5 (mangga lokal dari gedonggincu) adalah 79%, MG1 (lokal dari golek mangga) dan Mg3 (mangga lokal arummanis) adalah 75 %.

Growth and Protein Content Establishment of Pleurotus ostreatus on Liquid and Solid Medium Mumpuni, Aris; Ekowati, Nuraeni; Purnomowati, Purnomowati; Purwati, Endang Sri
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11660

Pleurotus ostreatus cultivation is performed using solid medium to harvest fruit body and using liquid medium to harvest mycelia in submerged culture. Modifying nutrients in the medium to increase protein content of the fruitbody and mycelia can be done through addition of nitrogen-containing materials. This study aims to determine: the appropriate composition of the liquid medium for high mycelial growth and protein content; and the exact composition of the solid medium to obtain high fruitbody product and protein content. The method was experimental with completely randomized design (CRD). The treatments were incubation of P. ostreatus on three types of liquid medium and four types of solid medium. The results showed that the optimal liquid medium composition for mycelial growth was Liquid Fermentation Medium 1 (FC1) with 10% corn fluor, and the highest protein content was in Liquid Fermentation Medium 2 (FC2 = 29.76%). While the optimal solid medium composition for fruitbody production was the medium with 3% corn starch supplement (TJ3), and the highest protein content was obtained from the medium without corn starch supplement (TJ0=24.69%). The increase of mycelial and fruitbody weight from the medium with the addition of corn material indicated a prospective in cultivation process, however effort to increase protein content of the fruit body needs further research. Cultivating P. ostreatus in mycelial phase may take shorter incubation time, may be produced in mass production with less space consuming, and higher protein content than that by producing fruitbody.

Antioxidant Potential of Ethanol and Ethyl Acetat Extract of Ganoderma sp. Mycelium Ratnaningtyas, Nuniek Ina; Purnomowati, Purnomowati; Purwati, Endang Sri; Septiana, Aisyah Tri; Ekowati, Nuraeni; Supriyadi, Adi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11512

Ganoderma sp. Banyumas 1 isolate that reffered as Ganoderma sp. is a new discovered isolate from Banyumas, Central Java, Indonesia expected to have a potential properties of antioxidant of medicinal mushroom. This study aimed to determine the antioxidant potential and the appropriate solvent for itâs extracting from Ganoderma sp. This research result showed that ethyl acetate was able to extract as many as 15.57%, while etanol was only able to extract 3.87%Â active compounds from dried 28 days old Ganoderma sp. mycelium cultivated in the Mushroom Complete Medium (MCM). Extract of ethyl acetate (non-polar) extraction of mycelium of Ganoderma sp. had a potential character as an antioxidant source and performed a better result than from ethanolic (polar) extraction as shown in the IC50 value. Extract from ethyl acetate extraction had an average IC50 value smaller than Â from ethanolic extract (581.80 < 1285.67). Extract from ethyl acetate extraction resulted in a higher amount of phenol than that ethanolic extract 29.23 < 57.67. Inhibition percentage of both extracts at 65% was known to occur at concentration of 1000 ppm for ethyl acetate extract and 2000 ppm for ethanolic extract. An important finding was that ethyl acetate can be used as appropriate solvent for extracting antioxidant compound better than ethanolic. In conclusion, the mycelium extract of Ganoderma sp. extracted with ethyl acetate and ethanol as solvent is potential to be used as a source of natural antioxidants. This research result has benefit in developing potency of local resources as herbal resources.

ANTHRACNOSE DISEASE ON VEGETABLES CROPS IN SERANG VILLAGE, DISTRICT OF KARANGREJA, PURBALINGGA REGENCY Sucianto, Eddy Tri; Abbas, Muachiroh; Purwati, Endang Sri
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.21818

Various vegetables are widely cultivated in Serang Village, District of Karangreja, Purbalingga Regency. This village is located at 1350 above sea level. Vegetable productions in Serang are often constrained by the presence of diseases caused by pathogenic fungi Colletotrichum sp. called anthracnose. However, there was no data regarding anthracnose attack in vegetable crops in Serang. This study aimed to find out the types of vegetable that were attacked by anthracnose disease as well as the intensity of the attack in a vegetable crops in Serang Village, Karangreja District, Purbalingga Regency. Vegetables with anthracnose were purposively selected from 10 different locations. The anthracnose disease was identified based on their symptoms and analyzed descriptively. The results showed that five different vegetable crops suffer from anthracnose, and the intensity of anthracnose ranges from 32% up to 52%. Those intensity percentages indicate that large part of vegetable crops in Serang Village was attacked by anthracnose and possibly cause a significant decrease in vegetable production in that village. Our results provide the first scientific data about anthracnose attack in Serang Village. The data is essential for the management of vegetable crops in Serang Village.

Pemberian Inokulum Fungi Mikoriza Arbuskula (FMA) Campuran terhadap Kemunculan Penyakit Layu Fusarium pada Tanaman Semangka [Citrullus lanatus (Thunb.) Matsum. & Nankai] Berbiji dan Non Biji Febriyana, Riska; Dwiputranto, Uki; Purwati, Endang Sri
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.2006

Watermelon (Citrullus lanatus) is a plant originating from the dry region of North Africa and is now cultivated in almost all regions of the world as a fruit that has high economic value. Efforts to cultivate and breed watermelons become very important related to defense against disease. One of the dominant diseases in watermelon is fusarium wilt disease caused by Fusarium oxysporum. Mycorrhiza is a symbiotic mutualism between certain fungi and higher plants. Arbuscular mycorrhizal fungi (AMF) can be used as an alternative in reducing fusarium wilt effect. The purpose of this research is to know the effect and effective dose of AMF mixture inoculum to reduce Fusarium wilt disease in seeded and seedless watermelon. This research used a completely randomized design (CRD) with different doses of mixed AMF inoculums (0, 5, 10, 15, 20 g) AMF with zeolite/plant carrier medium. The main parameters discussed are the disease intensity and disease incubation period, while the supporting parameters observed were pH, temperature, humidity, and degree of infection. The data obtained were analyzed using Variance Test (F test) with a Standard Error of 5%. The results of this research shows that there is effect of inoculation AMF mixture inoculum to reduce Fusarium wilt disease in seeded and seedless watermelon. The effective dose of AMF mixture to reduce the Fusarium wilt disease in seeded watermelon is inoculation AMF mixture dose 10 g / plant and inoculation AMF mixture dose 15 g/ plant in seedless watermelon.

Genera Jamur Patogen dan Persentase Penyakit Bercak Daun yang ditemukan pada Pertanaman Sawi Hijau (Brassica juncea) di Desa Serang, Kecamatan Karangreja, Purbalingga Hartatik, Nia Sri; Sucianto, Eddy Tri; Purwati, Endang Sri
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3387

Green mustard (Brassica juncea) is an annual or horticultural plant of the Brassica genus. The purpose of this research is to knowledge the type of fungi that cause leaf spot disease on green mustard plants and to know the large percentage of leaf spot diseases caused by fungus on mustard greens in Serang Village, Karangreja District, Purbalingga. This research is used survey method by taking purposive random sampling at two different locations. The type of fungus that causes leaf spot disease on (B. juncea) mustard green can be identified by isolating the part of plants are sick then identified. Identification of fungi is doing by two stages of observation, that is observation macromorphological characters and micromorphological characters. Observation of macromorphological characters includes colony color, colony shape, the edge of colony shape and colony reverse color. Observation of the micromorphology character includes the presence of hyphae, hyphae (insulated or non-insulated), conidia shape, conidia color, additional devices and conidiophores. The next stage to find out the type of fungus that causes leaf spot disease is Koch's Postulate.The results obtained there is one type of disease in green mustard plants. Leaf spot is one of the diseases of mustard greens caused by the fungus Alternaria sp, with a frequency of leaf spot disease as many as 196 times, and the percentage of disease of 51,30%.

Identifikasi Jamur Penyebab Penyakit pada Tanaman Sawi Putih (Brassica rapa L.) dan Persentase Penyakitnya di Desa Serang Kecamatan Karangreja, Kabupaten Purbalingga Apriliani, Siti Hanifah Nur; Sucianto, Eddy Tri; Purwati, Endang Sri
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 4 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3394

Sawi putih (Brassica rapa L.) adalah sekelompok tumbuhan dari marga Brassica yang dimanfaatkan daunnya sebagai bahan pangan (sayuran), baik segar maupun diolah. Sawi putih termasuk ke dalam tanaman hortikultura yang memiliki nilai ekonomis tinggi. Beberapa spesies dari genus Brassica memiliki kemiripan satu sama lain. Sawi memiliki kandungan nutrisi seperti kalsium, asam folat, dan magnesium serta vitamin A, B, C, E, dan K. Petani di desa Serang memiliki masalah tidak hanya di bidang biaya produksi, tetapi juga pernah mengalami kegagalan panen akibat penyakit yang disebabkan oleh mikroorganisme, sehingga perlu adanya pengendalian untuk meningkatkan produktivitas tanaman sawi putih. Penelitian ini bertujuan untuk mengetahui jenis-jenis penyakit yang disebabkan oleh jamur pada tanaman sawi putih, mengetahui jamur yang menyebabkan penyakit pada tanaman sawi putih serta mengetahui persentase kemunculan jamur penyebab penyakit pada tanaman sawi putih di desa Serang Kecamatan Karangreja, Kabupaten Purbalingga. Penelitian ini menggunakan metode survey dengan pengambilan sampel secara purposive random sampling pada dua lokasi yang berbeda di Desa Serang Kecamatan Karangreja, Kabupaten Purbalingga. Identifikasi penyakit dilakukan dengan melihat tanda serta gejala secara makroskopis, sedangkan jamur penyebab penyakit diamati karakter mikromorfologi dan makromorfologinya. Identifikasi, dilanjutkan dengan Postulat Koch. Hasil penelitian di Desa Serang Kecamatan Karangreja, Kabupaten Purbalingga didapatkan jenis penyakit pada tanaman sawi putih adalah penyakit layu daun dan bercak daun. Penyakit layu daun disebabkan oleh jamur Fusarium sp. dan penyakit bercak daun disebabkan oleh Botryodiplodia sp. Penyakit yang paling banyak muncul pada tanaman sawi putih yaitu penyakit layu daun oleh Fusarium sp dengan frekuensi kemunculan sebanyak 199 kali dan persentase penyakit sebesar 50,95% dan frekuensi kemuncula untuk bercak daun Botryodiplodia sp. sebanyak 70 kali dan persentase penyakit sebesar 17,9%.

Pengaruh Kadar Garam terhadap Karakter Anatomi Daun Kedelai [Glycine max (L.) Merr.] Kultivar Grobogan Setiana, Devi Vira; Juwarno, Juwarno; Purwati, Endang Sri
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 1 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2023.5.1.4735

Kedelai [Glycine max (L.) Merr.] saat ini kebutuhannya semakin meningkat namun tidak diimbangi oleh produksinya. Kegagalan produksi tersebut salah satunya akibat kurangnya lahan. Tujuan penelitian ini adalah untuk mengetahui pengaruh kadar garam terhadap karakter anatomi daun kedelai kultivar Grobogan. Penelitian ini menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL). Parameter yang diamati meliputi tebal epidermis adaksial dan abaksial, tebal kutikula adaksial dan abaksial, tebal mesofil, panjang stomata, lebar stomata, kerapatan stomata, kerapatan trikomata. Data dianalisis dengan analisis ragam (ANOVA), dengan tingkat kesalahan 5%. Perlakuan yang memberikan pengaruh nyata, dilanjutkan dengan uji Beda Nyata Jujur (BNJ) dengan Standar Kesalahan 5%. Hasil penelitian menunjukkan kadar garam 100 mM dapat meningkatkan ukuran tebal epidermis adaksial dan epidermis abaksial. Kadar garam 0 mM meningkatkan panjang stomata abaksial dan kerapatan stomata adaksial.

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