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All Journal Majalah Obat Tradisional Jurnal Bioteknologi & Biosains Indonesia (JBBI) Biosaintifika: Journal of Biology & Biology Education BioEksakta : Jurnal Ilmiah Biologi Unsoed Journal of Science and Technology Research for Pharmacy Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Nuraeni Ekowati, Nuraeni
Faculty of Biology, University of Jenderal Soedirman, Purwokerto, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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DAYA ANTIMIKROBA METABOLIT BIOAKTIF JAMUR SHIITAKE (Lentinula edodes (Berk.) Pegler) YANG DIKULTUR PADA TIGA JENIS MEDIUM FERMENTASI Ekowati, Nuraeni; Kasiamdari, Rina Sri; Pusposendjojo, Nursamsi; Soegihardjo, C.J.
Majalah Obat Tradisional Vol 16, No 3 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.742 KB) | DOI: 10.14499/mot-TradMedJ16iss3pp%p

Abstract

Jamur shiitake (Lentinula edodes (Berk.) Pegler) merupakan salah satu jamur yang berpotensi sebagai jamur pangan dan bahan obat (edible and medicinal mushroom). Kultivasi pada medium cair dengan proses fermentasi telah dikembangkan selama beberapa tahun terakhir ini. Empat isolat L. edodes (isolat asal Malang, Cianjur, Lembang dan Yogyakarta) dikultur pada tiga jenis medium fermentasi yaitu (KM: Kauffman Medium; GYMT: Glucose, Yeast extract,  Malt extract, Thiamin; YEMR: Yeast extract,  Malt extract,  Rice bran). Metabolit bioaktif yang diekstrak dari biomassa miselium dan filtrat kultur hasil fermentasi diuji menggunakan mikroba patogen Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218, Candida albicans ATCC 10231,dan  Trichophyton mentagrophytes. Data diameter zona  hambat dianalisis menggunakan analisis ragam, dan dilanjutkan dengan uji Duncan pada tingkat kesalahan 5 %. Hasil penelitian menunjukkan bahwa senyawa bioaktif dalam filtrat kultur maupun biomassa miselium L.edodes yang diekstrak menggunakan kloroform, etil asetat dan air, mampu menghambat S. aureus, E.coli dan C. albicans tetapi tidak mampu menghambat T.mentagrophytes. Isolat L.edodes asal Lembang dan Yogyakarta yang dikultur pada medium Kauffman memberikan hasil zona hambat terbaik (24,97-31,14 mm). Mikroba uji yang paling sensitif terhadap senyawa bioaktif dari L. edodes adalah C. albicans diikuti oleh E.coli, S. aureus dan T. mentagrophytes. Metabolit bioaktif dari L. edodes berpotensi menghambat mikroba patogen dari kelompok bakteri dan khamir tetapi tidak menghambat jamur.  
REVIEW: POTENSI MIKOREMEDIASI LOGAM BERAT Kurniawan, Andri; Ekowati, Nuraeni
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 3, No 1 (2016): June 2016
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.458 KB) | DOI: 10.29122/jbbi.v3i1.21

Abstract

Heavy metals contamination is a main issue which has negative impacts to environment and organisms. Various methods have been developed to reduce such pollutants, including utilization of organisms’ capability in order to minimize the contamination. Mycoremediation is one of remediation process in contaminated environment using fungi and its reduction mechanisms, involving intracellular, as well as extracellular system. There are some species of fungi that are frequently used as remediator agents, for example Aspergillus sp., Fusarium sp., Penicillium sp., Phanerochaete sp., Trichoderma sp. There are some methods that have been used for heavy metal reduction mechanisms such as biosorption, bioaccumulation, bioprecipitation, bioreduction, and bioleaching.Keywords: Mycoremediation, fungi, heavy metal, biosorption, bioaccumulation ABSTRAK Kontaminasi logam berat adalah suatu permasalahan utama yang berdampak negatif bagi lingkungan dan juga makhluk hidup. Berbagai metode telah dikembangkan untuk mereduksi cemaran, termasuk memanfaatkan kemampuan organisme untuk meminimalkan kontaminan tersebut. Mikoredemiasi adalah salah satu proses remediasi cemaran di lingkungan dengan melibatkan fungi beserta mekanisme reduksinya, baik secara intraselular maupun ekstraselular. Beberapa jenis fungi yang sering dijadikan agen remediator antara lain Aspergillus sp., Fusarium sp., Penicillium sp., Phanerochaete sp., Trichoderma sp. Beberapa prinsip yang digunakan untuk menghilangkan logam berat antara lain biosorpsi, bioakumulasi, biopresipitasi, bioreduksi, dan bioleaching. Kata kunci: Mikoremediasi, fungi, logam berat, biosorpsi, bioakumulasi
Growth and Protein Content Establishment of Pleurotus ostreatus on Liquid and Solid Medium Mumpuni, Aris; Ekowati, Nuraeni; Purnomowati, Purnomowati; Purwati, Endang Sri
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11660

Abstract

Pleurotus ostreatus cultivation is performed using solid medium to harvest fruit body and using liquid medium to harvest mycelia in submerged culture. Modifying nutrients in the medium to increase protein content of the fruitbody and mycelia can be done through addition of nitrogen-containing materials. This study aims to determine: the appropriate composition of the liquid medium for high mycelial growth and protein content; and the exact composition of the solid medium to obtain high fruitbody product and protein content. The method was experimental with completely randomized design (CRD). The treatments were incubation of P. ostreatus on three types of liquid medium and four types of solid medium. The results showed that the optimal liquid medium composition for mycelial growth was Liquid Fermentation Medium 1 (FC1) with 10% corn fluor, and the highest protein content was in Liquid Fermentation Medium 2 (FC2 = 29.76%). While the optimal solid medium composition for fruitbody production was the medium with 3% corn starch supplement (TJ3), and the highest protein content was obtained from the medium without corn starch supplement (TJ0=24.69%). The increase of mycelial and fruitbody weight from the medium with the addition of corn material indicated a prospective in cultivation process, however effort to increase protein content of the fruit body needs further research. Cultivating P. ostreatus in mycelial phase may take shorter incubation time, may be produced in mass production with less space consuming, and higher protein content than that by producing fruitbody.
Effectiveness of Pleurotus ostreatus Extract Through Cytotoxic Test and Apoptosis Mechanism of Cervical Cancer Cells Ekowati, Nuraeni; Mumpuni, Aris; Muljowati, Juni Safitri
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.7546

Abstract

Pleurotus ostreatus is a common mushroom cultivated in Indonesia, and potential properties of bioactive compounds for medicinal mushroom. This study was aimed at obtaining P.ostreatus extract bioactive compounds potential in inhibiting the proliferation of cervical cancer cells (HeLa) and evaluating the HeLa cell proliferation kinetics and HeLa cell death mechanisms. The research was beneficial in making this product can be easily applied in a more controlled industrial scale. Anticancer activity test through a cytotoxic test using the MTT [3- (4,5-dimetiltiazol-2-yl) -2.5-diphenyl tertrazolium bromide], the kinetics proliferation of HeLa cells and HeLa cell death mechanism was performed. Linear regression analysis was used to analyze the data. Ethyl acetate extract of P. ostreatus isolated from Madiun showed the best results with IC 50 = 107.59 g / ml. HeLa cell proliferation kinetics analysis showed that the application of bioactive compounds 100 g / ml resulted in an increase of in death of HeLa cells along with length of incubation time. An important finding was that HeLa cells death by apoptosis was greater than by necrosis. In conclusion, the extracts of P. ostreatus has the potential to inhibit the growth of HeLa cells.
Antioxidant Potential of Ethanol and Ethyl Acetat Extract of Ganoderma sp. Mycelium Ratnaningtyas, Nuniek Ina; Purnomowati, Purnomowati; Purwati, Endang Sri; Septiana, Aisyah Tri; Ekowati, Nuraeni; Supriyadi, Adi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11512

Abstract

Ganoderma sp. Banyumas 1 isolate that reffered as Ganoderma sp. is a new discovered isolate from Banyumas, Central Java, Indonesia expected to have a potential properties of antioxidant of medicinal mushroom. This study aimed to determine the antioxidant potential and the appropriate solvent for it’s extracting from Ganoderma sp. This research result showed that ethyl acetate was able to extract as many as 15.57%, while etanol was only able to extract 3.87% active compounds from dried 28 days old Ganoderma sp. mycelium cultivated in the Mushroom Complete Medium (MCM). Extract of ethyl acetate (non-polar) extraction of mycelium of Ganoderma sp. had a potential character as an antioxidant source and performed a better result than from ethanolic (polar) extraction as shown in the IC50 value. Extract from ethyl acetate extraction had an average IC50 value smaller than  from ethanolic extract (581.80 < 1285.67). Extract from ethyl acetate extraction resulted in a higher amount of phenol than that ethanolic extract 29.23 < 57.67. Inhibition percentage of both extracts at 65% was known to occur at concentration of 1000 ppm for ethyl acetate extract and 2000 ppm for ethanolic extract. An important finding was that ethyl acetate can be used as appropriate solvent for extracting antioxidant compound better than ethanolic. In conclusion, the mycelium extract of Ganoderma sp. extracted with ethyl acetate and ethanol as solvent is potential to be used as a source of natural antioxidants. This research result has benefit in developing potency of local resources as herbal resources.
Effects of Ganoderma lucidum Extract on Diabetic Rats Ratnaningtyas, Nuniek Ina; Hernayanti, Hernayanti; Andarwanti, Suci; Ekowati, Nuraeni; Purwanti, Endang Sri; Sukmawati, Dalia
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (342.999 KB) | DOI: 10.15294/biosaintifika.v10i3.15356

Abstract

Diabetes mellitus (DM) is a metabolic syndrome which occurs when insulin is insufficiently produced or insulin cannot well serve its function. Diabetes is marked with increase in blood glucose level followed by increase in glycosylated hemoglobin level and decrease in insulin level. This research aims to examining the effect of Ganoderma lucidum extract on the blood glucose, insulin and glycosylated hemoglobin (HbA1c) level of diabetic white rat and determining the most effective dose of extract to be a diabetic agent. This research was experimentally conducted by employing Completely Randomized Design (CRD) with 6 treatments and 4 repetitions. The treatment groups consisted of healthy rats group (K1), rats with diabetes as negative control (K2), diabetic rats with the administration of metformin 45 mg/kg BW as the comparison (K3) and diabetic rats with the administration of G. lucidum extract with dose of 250, 500 and 1000 mg /kg BW (K4, K5 and K6 respectively). Blood glucose level examination was conducted after the alloxan induction with single dose of 125 mg/kg BW by intraperitoneal injection. The results show that mushroom G. lucidum extract administration with dose of 1000 mg/kg BW (K6) is the best dose to be an anti-diabetic agent. The benefit of the research is developing anti-diabetic agent from herbal resources.
Antidiabetic Potentials of Button Mushroom (Agaricus bisporus) on Alloxan-Induced Diabetic Rats Ekowati, Nuraeni; Yuniati, Nilasari Indah; Hernayanti, Hernayanti; Ratnaningtyas, Nuniek Ina
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.458 KB) | DOI: 10.15294/biosaintifika.v10i3.17126

Abstract

Button mushrooms (Agaricus bisporus) is an edible mushroom that is most widely cultivated in the world. It contains bioactive compounds that might provide beneficial effects on diabetes mellitus patient. The study aimed to determine the effects of A. bisporus administration on the blood glucose, and malondyaldehyd (MDA) levels as well as superoxide dismutase (SOD) activity of alloxan-induced diabetic rats. This study was also conducted to determine the secondary metabolites produced by A. bisporus. The method used was experimental methods with Completely Randomized Design. A. bisporus extract at the doses of 250, 500 and 750 mg/kg body weight (BW) per day were orally applied to alloxan-induced diabetic rats for a period of 14 days after the rats became diabetes. The results showed that the extract of A. bisporus could decrease blood glucose, and MDA levels as well as increase SOD activity (p < 0.05). A. bisporus extract 500 mg/kg BW is the most effective dose to be used. Based on Thin Layer Chromatography (TLC) test, it was known that secondary metabolites produced by A. bisporus are flavonoids, alkaloids, terpenoids and saponins. A. bisporus has potential as an antidiabetic through the ability to decrease blood glucose, and MDA levels, as well as increase SOD activity in diabetic rats. This research is able to provide information about the antidiabetic potential of A. bisporus extract so that it can be used as an alternative natural antidiabetic agent and can be applied in the community with ease and in a more controlled industrial scale.
The Cytotoxic and Apoptosis Effects of Chloroform Extracts of Auricularia auricula on Cervical Cancer Cells Hikam, Arif Rahman; Ekowati, Nuraeni; Hernayanti, Hernayanti
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.336 KB) | DOI: 10.15294/biosaintifika.v11i1.15492

Abstract

Auricularia auricula is an edible mushroom cultivated in Indonesia that has been known to have potential properties of bioactive compounds than can be used for medicinal purpose. This study aimed to examine the cytotoxic and apoptosis effect of chloroform extract of A. auricula on cervical cancer cells in vitro. The research design was in vitro experimental research. Cytotoxic tests was using an MTT [3-(4,5-dimetiltiazol-2-yl)-2.5-diphenyl tertrazolium bromide] assay and apoptosis test was using double staining method. Test of bioactive compounds was carried out using GCMS. Cytotoxic effect were analyzed by linear regression and apoptosis test was analyzed descriptively. Chloroform extract of mycelium A. auricula showed the best results with IC50 = 264.87 µg/ml. An important finding obtained after the double staining process was that chloroform extract of A. auricula can induce HeLa cells death by apoptosis. GCMS test results showed that the extracts containing limonene and piperidinone which are the anticancer bioactive compounds. In conclusion, the chloroform extracts of A. auricula has the potential to inhibit the growth of cervical cancer cells. The benefit of this study are expected to provide information about the anticancer potential of extract of A. auricula against cervical cancer cells, thus contributing to the development of alternative anticancer treatments from natural product.
REVIEW: POTENSI MIKOREMEDIASI LOGAM BERAT Kurniawan, Andri; Ekowati, Nuraeni
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 1 (2016): June 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.458 KB) | DOI: 10.29122/jbbi.v3i1.21

Abstract

Heavy metals contamination is a main issue which has negative impacts to environment and organisms. Various methods have been developed to reduce such pollutants, including utilization of organisms’ capability in order to minimize the contamination. Mycoremediation is one of remediation process in contaminated environment using fungi and its reduction mechanisms, involving intracellular, as well as extracellular system. There are some species of fungi that are frequently used as remediator agents, for example Aspergillus sp., Fusarium sp., Penicillium sp., Phanerochaete sp., Trichoderma sp. There are some methods that have been used for heavy metal reduction mechanisms such as biosorption, bioaccumulation, bioprecipitation, bioreduction, and bioleaching.Keywords: Mycoremediation, fungi, heavy metal, biosorption, bioaccumulation ABSTRAK Kontaminasi logam berat adalah suatu permasalahan utama yang berdampak negatif bagi lingkungan dan juga makhluk hidup. Berbagai metode telah dikembangkan untuk mereduksi cemaran, termasuk memanfaatkan kemampuan organisme untuk meminimalkan kontaminan tersebut. Mikoredemiasi adalah salah satu proses remediasi cemaran di lingkungan dengan melibatkan fungi beserta mekanisme reduksinya, baik secara intraselular maupun ekstraselular. Beberapa jenis fungi yang sering dijadikan agen remediator antara lain Aspergillus sp., Fusarium sp., Penicillium sp., Phanerochaete sp., Trichoderma sp. Beberapa prinsip yang digunakan untuk menghilangkan logam berat antara lain biosorpsi, bioakumulasi, biopresipitasi, bioreduksi, dan bioleaching. Kata kunci: Mikoremediasi, fungi, logam berat, biosorpsi, bioakumulasi
The Antibacterial Activity of Acanthus ilicifolius L. n-Hexane Fraction warsinah, warsinah; Wijaya, Triyadi Hendra; Ekowati, Nuraeni
Journal of Science and Technology Research for Pharmacy Vol 1 No 2 (2021)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jstrp.v1i2.49615

Abstract

Background: Recently, with the high prevalence of diarrhoea caused by bacterial infection, the usage of antibiotics has increased. Antibiotic overuse might lead to several side effects and resistance, suggesting the development of an alternate antibacterial agent. A mangrove plant, Acanthus Illinois, contained triterpenoid, which has antibacterial properties. Aim: This study aimed to evaluate the antibacterial effect of the Acanthus Illinois n-hexane fraction against Escherichia coli and Shigella dysenteriae. Method: The Acanthus Illinois was fraction using n-Hexane, identification of secondary metabolite compound using GC-MS, and evaluation of antibacterial activity against Escherichia coli and Shigella dysenteriae under paper disc methods. This study was designed using a fully randomized design (CRD) with concentrations of fractions 1%, 2%, and 4%. The GC-MS results were compared to the WILEY 9 library and analysed. A prism graph was used to measure the inhibition zone of antibacterial activity. Result: The n-hexane fraction yield is 3.3% and contains sesquiterpene compounds (trans (beta.)-caryophyllene, alpha humulene, naphthalene decahedron-4A-methyl), terpenoid alcohol (3,7,11,15-tetramethyl -2-hexadecane-1-ol), and fatty acids (hexadecanoic acid methyl ester. Acanthus Illinois n-hexane fraction has antibacterial activity against Escherichia coli and Shigella dysenteriae in a dose-dependent manner. Conclusion: The n-hexane fraction of leaves Acanthus ilicifolius contains sesquiterpene, alcohol terpenoids, and fatty, and has antibacterial activity against Escherichia coli and Shigella dysenteriae
Co-Authors . HERNAYANTI Abd. Rasyid Syamsuri Ade Erma Suryani, Ade Erma Ainillah, Awwaluz Zahroh Mahya Aisyah Tri Septiana Amanda, Safira Amelia, Aurora Pradipta Andarwanti, Suci Apriyani, Tri Rahayu Aris Mumpuni C.J. SOEGIHARDJO Dalia Sukmawati Endang Sri Purwanti Endang Sri Purwati Feryawan, Feryawan hasanah, Amanda rohmatun I Gusti Wayan Murjana Yasa Juni Safitri Muljowati Maharani, Amalia Sofia MU'AFA, RAFID HILMY Mubarrak, Syifa Nuniek Ina Ratnaningtyas Nursamsi Pusposendjojo, Nursamsi Purnomowati Purnomowati, Purnomowati Rahmadianti, Maghfira Rizki Ratnaningtyas, ‪Nuniek Ina Rina Sri Kasiamdari Supriyadi, Adi Triyadi Hendra Wijaya Wardaya, Yasenia Sandra Warsinah Warsinah Yuniati, Nilasari Indah