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All Journal Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Jurnal Kesehatan Masyarakat Jurnal Kesehatan Masyarakat (JKM) CENDEKIA UTAMA Indonesian Journal of Biotechnology Jurnal Farmasi Indonesia Jurnal Biomedika Jurnal Teknologi Hasil Pertanian SULUH: Jurnal Abdimas Jurnal Pengabdian Kesehatan Proceeding Biology Education Conference Jurnal Kesehatan Kusuma Husada Journal of Advanced Nursing and Health Sciences
Agnes Sri Harti
Universitas Kusuma Husada Surakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Education Engineering Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Veterinary Other

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Detection of eae, bfpA, espA Genes on Diarrhoeagenic Strains of Escherichia coli Isolates Harti, Agnes Sri; Iravati, Susi; Asmara, Widya
Indonesian Journal of Biotechnology Vol 11, No 1 (2006)
Publisher : Universitas Gadjah Mada

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Abstract

The Enteropathogenic Escherichia coli (EPEC) is one of pathogenic strain of diarrheagenic E. coli group in children andinfant that occurs in developing countries. The significant virulence factors in pathogenic EPEC are eaeA (E. coli attachingeffacing), bfpA (bundle-forming pilus A) and espA (encoding secreted protein A) genes. The use of DNA probes to detect thevirulence genes in E. coli in Indonesia is not common yet. In this experiment the gene fragments of eae, bfpA, and espA were usedas probes to detect the EPEC among E. coli isolates from stool specimensin of diarrheic children attending Public Health Centersin Yogyakarta. The DNA samples were isolated from 49 diarrheagenic E. coli isolates. The DNA probes of eae, bfpA and espAwere obtained by amplification of DNA fragment of EPEC O126 using PCR technique. Furthermore, those probes were used toidentify the presence of those genes among E. coli isolates using hybridization technique. The results showed that 42 (85.7%)isolates were espA+, 25 isolates (51%) were eaeA+ (EPEC strains). Therefore among 25 isolates of EPEC, 20 isolates (80 %)among EPEC were bfpA+ (typical EPEC strains).Keywords : DNA probe, eae, bfpA, espA, EPEC.
Anti-Tuberculosis Drugs against the Resistance Level of Mycobacterium tuberculosis isolates Sutanto, Yusup Subagio; Sutanto, Magdalena Sutanto; Harti, Agnes Sri
KEMAS: Jurnal Kesehatan Masyarakat Vol 17, No 1 (2021)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v17i1.24158

Abstract

Prolonged MDR-TB therapy can have side effects, namely a correlation between cure rates and changes in bacterial profiles related to resistance to anti-tuberculosis drugs (ATD) which can affect the incidence rate of MTB and MDR-TB in a region. The research objective was to analyze the effectiveness of the type of ATD against the resistance level of Mycobacterium tuberculosis (MTB) isolates with the incidence of tuberculosis (TB) and MDR-TB. The research method used was a retrospective cohort based on tracing medical record data at the Surakarta City Center General Hospital 2016 until 2017 with total sampling. The independent variable of this study was the type of ATD, while the dependent variable was the resistance level of MTB isolates. The characteristics of the most patient respondent suspect TB were male with the level of resistance of MTB isolates to ATD relatively varied. The results of the analysis of different tests showed a p value of 0.000 so that the p value was <0.05, so there was an effect of the type of ATD (Strepttomycin, Isoniazid, Rifampicin and Ethambutol) on the resistance of TB isolates from patients with suspected TB. This is useful to determine the success of TB therapy in terms of mortality and the effectiveness of therapy in TB patients.
The Sensitivity Test of Mycobacterium tuberculosis to Snail Seromucoid and Chitosan in vitro Yusup Subagio Sutanto; Magdalena Sutanto; Agnes Sri Harti; Nony Puspawati
Biomedika Vol 14 No 1 (2021): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v14i1.1128

Abstract

Tuberculosis (TB) is an infection caused by M. tuberculosis (MTb) and is transmitted through droplets of phlegm in the air from patients or those suspected of having TB. In general, treatment for TB is done with anti-tuberculosis drugs (ATDs), specifically streptomycin, isoniazid, rifampicin, and ethambutol (SIRE) that takes a long time due to the level of resistance of MTb bacteria. The resistance of MTb triggers ATDs based on natural bioactive compounds. Chitosan as a result of chitin deacetylation can function as an antimicrobial agent because it is polycationic, which is biodegradable, biocompatible, and non-toxic. Snail (Achatina fulica) seromucoid contains antibacterial bioactive compounds, namely glycans, peptides, glycopeptides, achasin protein, and chondroitin sulfate. This study aims at testing the sensitivity of MTb isolates against snail seromucoid and chitosan in vitro. This research applied the experimental research method. MTb isolates were obtained from sputum samples of patients suspected of TB at the Surakarta Regional Public Hospital (RSUD Surakarta). The results of screening for MTb were positive, based on the microscopic examination of MTb using the Ziehl Nelson (ZN) method, the MPT 64 rapid test, and the quick molecular test using the Genexpert method. The research was completed through several stages, including the preparation of a suspension of germs with a concentration of 1 mg/ml or Mc. Farland 0.5-1.0; preparation of the stock solution and working solution (WS); drug sensitivity test (DST) against snail seromucoid; chitosan and ATDs (SIRE) on Lowenstein Jensen (LJ) media; and incubation at 37°C for 3-4 weeks. The results were interpreted on day 28 or day 42. The results have revealed that MTb isolates are 100% resistant to snail seromucoid and 2% chitosan. This study concludes that MTb isolates from suspected TB are resilient to 100% snail seromucoid and 2% chitosan.
PENGARUH MIRROR THERAPY TERHADAP KEKUATAN OTOT EKSTREMITAS PADA PASIEN STROKE DI RSUD dr. MOEWARDI Setiyawan Setiyawan; Pipit Siti Nurlely; Agnes Sri Harti
JKM (Jurnal Kesehatan Masyarakat) Cendekia Utama Vol 6, No 2 (2019): JKM (Jurnal Kesehatan Masyarakat) Cendekia Utama
Publisher : STIKES Cendekia Utama Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (611.38 KB) | DOI: 10.31596/jkm.v6i2.296

Abstract

Manifestasi klinis dari stroke pada umumnya mengalami kelemahan sebagian atau seluruh anggota gerak dari tubuh sehingga pasien tidak mampu melakukan aktivitas karena kelemahan anggota gerak dan membutuhkan latihan untuk mencegah kecacatan. Penatalaksanaan pada stroke adalah latihan rentang gerak sendi yang dilakukan kebanyakan pada fisioterapi. Intervensi yang bisa digunakan untuk peningkatan kekuatan otot dengan mengandalkan ilusi visual pasien dengan menggunakan media cermin yaitu mirror therapy. Penelitian ini bertujuan untuk mengetahui pengaruh mirror therapy terhadap kekuatan otot ekstremitas. Desain penelitian adalah quasy experiment pre post test with control group design. Populasi adalah pasien stroke iskemik dengan teknik consecutive sampling berjumlah 30 responden. Uji analisa menggunakan Wilcoxon Signed Ranks Test dan Mann Whitney-U Test. Hasil analisa Wilcoxon Testmenunjukkan terdapat perbedaan kekuatan otot ekstremitas sebelum dan sesudah diberi mirror therapy dan latihan ROM yaitu pada ekstremitas atas didapatkan nilai p=0,008 kelompok kontrol sedangkan kelompok intervensi p=0,002. Pada ekstremitas bawah didapatkan nilai p=0,083 kelompok kontrol sedangkan kelompok intervensi p=0,003. Uji statistik Mann Whitney pada ekstremitas atas diperoleh nilai p=0,004 sedangkan pada ekstremitas bawah diperoleh nilai p=0,001.Kesimpulan: ada pengaruh mirror therapy terhadap kekuatan otot pada pasien stroke sehingga dapat dipertimbangkan sebagai salah satu tambahan tindakan keperawatan untuk meningkatkan kekuatan otot dan memperbaiki fungsi motorik. Kata kunci: Kekuatan Otot, Mirror Therapy, Stroke
POTENSI CHITO-OLIGOSACCHARIDE (COS) SEBAGAI PREBIOTIK DAN PENGAWET ALAMI DALAM PEMBUATAN TAHU SINBIOTIK Agnes Sri Harti; Anis Nurhidayati; Desi Handayani
Prosiding SNST Fakultas Teknik Vol 1, No 1 (2013): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 4 2013
Publisher : Prosiding SNST Fakultas Teknik

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Abstract

Tahu  merupakan  salah  satu  produk  olahan  kedelai  yang  mengandung  protein,  lemak, karbohidrat dan  serat; dibuat melalui proses penggumpalan protein sehingga berbentuk  semi padat.  Tahu  dikenal  sebagai  produk  pangan  yang  tidak  awet  dan  proses  pembuatannya umumnya  dilakukan  secara  konvensional  atau  tradisional  dari  segi  peralatan,  metode  dan pemasarannya.  Oleh  karena  itu  berbagai  upaya  dan  inovasi  dilakukan,  diantaranya mengawetkan  tahu  yang  berfungsi  memperpanjang  masa  simpan. Salah  satu  pengawet  alami adalah Chito-OligoSakarida (COS) yang berasal dari limbah perikanan yaitu kulit udang dan kepiting. COS sebagai senyawa turunan kitosan hasil proses deasetilasi kitin, memiliki ikatan 1,4  glukosamin  yang  bersifat    bersifat  antimikrobia  sehingga  mampu  berfungsi  sebagai pengawet. Hasil penelitian menunjukkan potensi Chito-oligosakarida (COS) sebagai prebiotik dan  pengawet  alami  dalam  produksi  tahu  sinbiotik. Pemanfaatan  COS  secara  terpadu  dalam produksi tahu sinbiotik diharapkan dapat multifungsi yaitu sebagai pengawet, prebiotik alami, antioksidan,    anti  hiperkolesterolemia,  antihiperglikemia,  imunostimulan  dan  adsorben sehingga  tahu  sinbiotik  dapat  berfungsi  sebagai  pangan  fungsional  yang  memberikan  nilai tambah  dari  aspek  teknologi,  ekonomi  maupun  sosial untuk  peningkatan  gizi  seseorang yang pada akhirnya dapat meningkatkan kesejahteraan. Kata kunci : Chito-OligoSakarida (COS), pengawet, prebiotik, tahu sinbiotik
TEMPE BEKATUL KITOSAN SEBAGAI BIOSUPLEMEN PREBIOTIK SEHAT ALAMI BAGI IBU HAMIL Aldila Puji Nugroho; Ika Murni W; Eka Reftiana Z; Agnes Sri Harti
Prosiding SNST Fakultas Teknik Vol 1, No 1 (2013): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 4 2013
Publisher : Prosiding SNST Fakultas Teknik

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Abstract

Kebutuhan  nutrien  bagi  ibu  hamil  berperan  penting  dalam  proses  pembentukan  janin  selama proses  kehamilannya  sehingga  diperlukan  asupan gizi  yang  cukup,  terutama  protein.  Tempe telah  dikenal  sebagai  pangan  fungsional  dengan  bahan  baku  kedelai  kuning  melalui  proses fermentasi jamur Rhizopus sp. Konsep fortifikasi pangan dipakai untuk karakterisasi makanan peningkatan  kesehatan  sebagai  biosuplemen  pangan  fungsional. Oleh  karena  itu  perlu dilakukan  berbagai  upaya  untuk  substitusi  biji  kedelai  kuning  menggunakan  bahan  baku  lain yang  aman,  bergizi  dan  ekonomis  . Tempe  bekatul  kitosan  sebagai  salah  satu  cara  fortifikasi pangan  fermentasi  tempe  yang  berbasis  protein  nabati  dan  diharapkan  dapat  digunakan sebagai  biosuplemen  prebiotik  sehat  alami  khususnya  bagi  ibu  hamil  yang  membutuhkan nutrisi  cukup  dan  seimbang  selama  kehamilan. Hasil  penelitian  menunjukkan penambahan bekatul  :  kedelai  (2:1)  dan Chito-oligosakarida  2%  b/b memberikan  hasil  organoleptis  yang optimum dalam  fermentasi  tempe. Pemanfaatan bekatul dan  kitosan dalam  fermentasi  tempe  sebagai  biosuplemen  prebiotik  alami  yang  bersifat  multifungsi   sehingga dapat  digunakan sebagai pangan fungsional  khususnya peningkatan gizi bagi ibu hamil. Kata kunci : bekatul, biosuplemen, kitosan, tempe.
IbM TEMPE BEKATUL PREBIOTIK Anis Nurhidayati; Agnes Sri Harti; Rahajeng Putriningrum
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 11, No 1 (2014): Prosiding Seminar Nasional XI Biologi
Publisher : Universitas Sebelas Maret

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Abstract

Rice brand of tempeh is one way of food fortification based fermented soy beans , which had been added with rice bran. Dependence on imported soybean production impact to high cost of raw materials and product. The concept of food fortification is used for the characterization of foods biosuplemen health improvement as a functional food . Therefore efforts should be done to substitute the yellow soybean seeds using other raw materials that are safe, nutritious and economical as was as rice bran and Chito - oligosaccharides ( COS ) . One form is a novelty that can be developed as biosuplemen prebiotic prebiotic natural healthy and highly nutritious . Krajan is one of the industrial centers tofu located in Kalurahan Mojosongo , District Jebres , Surakarta, Central Java Province . In this area there are more than 100 entrepreneurs tofu every day on average to process 250 kg of soybeans into tempeh . The UKM partners that will be used for this program are named UKM " Dele Mas " and UKM " Moelyo Santoso ". The purpose of the activities is to apply the COS and rice bran as a preservative and a natural prebiotic in the production of soybean bran on the partners to become a comprehensive program of activities and sustainable . Keywords: Community service program, rice bran, chitosan, tempeh
IbM USAHA KECAP DAN SAUS PREBIOTIK PEMANFAATAN CHITO-OLIOGOSACCHARIDE (COS) SEBAGAI PREBIOTIK DAN PENGAWET PADA KECAP DAN SAUS Agnes Sri Harti; Desy Handayani; Hutari Puji Astuti
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 11, No 1 (2014): Prosiding Seminar Nasional XI Biologi
Publisher : Universitas Sebelas Maret

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Abstract

UKM ketchup " Tomat Lombok " and UKM sauces " Sinar Mulia " is located in Penusupan street RT 04 RW Penusupan Village , District Kagok Slawi , Tegal city , Central Java Province . UKM ketchup " tomato Lombok " owned by Mr. HS Sumarnoto producing 1400 kg per day , whereas UKM sauce " Sinar Mulia " produces 500 kg of sauce per day . Total waste of two UKM to produce solid waste at 60 kg / day and 3 m3 of liquid waste . In generally produce soy sauce and sauce according to market needs and conventional manufacturing process so it is not durable or easily damaged due to microbial contamination in nature . Spoilage microorganisms in soy sauce is generally proteolytic bacteria or molds. Chito - oligosaccharides synthesis process ( COS ) from shrimp or crab shell waste that is relatively easy , simple ,  so that COS can be used as a prebiotic and a natural preservative and prebiotics for the production of ketchup and sauce  in UKM partner. The integrated COS utilization  ketchup and  sauce  is expected to function as a preservative , a natural prebiotic , antioxidant and adsorbent in wastewater treatment, so that  is expected to provide value -added aspects of the technology , economic and social through halal certificates and patents , which in turn can improve well-being . Keywords : UKM , ketchup and sauce, chito-oligosaccharide, prebiotics, preservatives
EFEK SINERGISTIK SINBIOTIK (CHITO-OLIGOSAKARIDA, BEKATUL DAN PROBIOTIK) SEBAGAI IMUNOSTIMULAN DALAM PANGAN FUNGSIONAL Agnes Sri Harti; Dwi Susi Haryati
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 10, No 1 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Universitas Sebelas Maret

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Abstract

Chito-oligosakarida (COS) merupakan glikoprotein ikatan 1,4 glukosamin, hasil deasetilasi kitin dari limbah perikanan yang sangat potensial dan berlimpah di Indonesia. COS mempunyai keunikan yaitu bersifat polikationik yang mampu melindungi protein dan menekan laju pertumbuhan bakteri patogen.  Bekatul (rice bran) sebagai hasil samping penggilingan padi, diperoleh dari lapisan luar karyopsis beras; mengandung karbohidrat, protein, mineral, lemak, vitamin B kompleks, inositol, fitat, gama orizanol, fitosterol, tokotrienol, asam amino, asam lemak tak jenuh, nitrilosid ( zat anti kanker) dan dietary fiber . Probiotik merupakan biopreparasi sel hidup atau organisme alami yaitu kelompok BAL (Bakteri Asam Laktat) yang mampu berkolonisasi  sebagai  microflora saluran cerna yang dapat menstimulir proses digestive  dan imunitas. Konsep sinergistik dan sinbiotik (prebiotik dan probiotik) akhir-akhir ini dipakai untuk karakterisasi makanan peningkatan kesehatan sebagai biosuplemen dalam pangan fungsional.  Tujuan penelitian mengetahui efek sinergistik sinbiotik  yaitu Chito-oligosakarida dan bekatul sebagai prebiotik alami dengan probiotik sebagai imunostimulan  dalam pangan fungsional.  Hasil penelitian menunjukkan potensi COS dan bekatul sebagai sumber prebiotik alami dan efek sinergistik sinbiotik (probiotik dan prebiotik) serta biopreparasinya dalam pangan fungsional sebagai biosuplemen yang aman tanpa menimbulkan residu, ekonomis dan multiguna khususnya di bidang kesehatan seperti  food additive, ’antibiotik alternatif’ dan imunostimulan. Kata kunci: Sinergistik, Sinbiotik, Imunostimulan,  Pangan Fungsional
Uji Aktivitas Antibakteri Salep Hidrokarbon Ekstrak Etil Asetat Daun Jengkol (Pithecollobium labatum Benth) terhadap Staphylococcus aureus ATCC® 25923 Ismi Rahmawati; Novia Yucca Tiara; Agnes Sri Harti
Jurnal Farmasi Indonesia Vol 6 No 3 (2009): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3358.742 KB) | DOI: 10.31001/jfi.v6i3.17

Abstract

Jengkol (Pethecollobium labatum Benth) leaf is a traditional medicine used to treat eczema, scabies, wound and ulcer, and the fruit peel is for sore. Jengkol leaf contains saponin, flavonoid, and tannin. The experiment was aimed to know the antibacterial activity of jengkol leaves ethyl acetate extract ointment against Staphylococcus aureus ATCC 25923 infection indicated by recovery time from infection on rabbit’s back with effective basic and concentration. Jengkol leaf’s ethyl acetate extract was then made into hydrocarbon basics ointments with concentrations of 10%, 20%, and 30%. The examination of the ointment consist of stability, homogenity, spreadability, adhesion capacity, protection capability, and viscosity test. The ointments were rubbed once a day on the infection area. The observation was carried out until the infection was healed which is confirmed by the disappearing of pus, drying wound, and when it was inoculated in Vogel Jhonson Agar media there was no growth of bacteria. The result of this experiment showed that the most effective ointment of jengkol leaves extract was ointment with 30% concentration. All the tests taken demonstrated that the ointment of jengkol leaves reached the standard of good ointment.
Co-Authors Aldila Puji N Aldila Puji Nugroho Amalia Agustin Anis Nurhidayati Anis Nurhidayati Bambang Abdul Syukur Desi Handayani Desy Handayani Dian Mudita Dwi Susi Haryati Dyah Yuliana E Estuningsih Eka Nursafitri Eka Reftiana Z Eka Reftiana Z Heni Nur Kusumawati Hutari Puji Astuti Ika Murni W Ika Murni W Intan Sari A Ismi Rahmawati Krisdianto, Muhammad Agung Magdalena Sutanto Martini Listrikawati Nony Puspawati Nora Idiawati Novia Yucca Tiara Pipit Siti Nurlely Rafika Sari Rahajeng Putriningrum Setiyawan Setiyawan Susi Iravati Sutanto, Magdalena Sutanto Utari K Widya Asmara Winda A. K. Yusup Subagio Sutanto Yusup Subagio Sutanto