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All Journal Jurnal SOLMA Bioedusiana: Jurnal Pendidikan Biologi Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika Likhitaprajna: Jurnal Ilmiah Fakultas Keguruan Ilmu Pendidikan Universitas Wisnuwardhana Malang Jurnal Promotif Preventif Afeksi: Jurnal Penelitian dan Evaluasi Pendidikan Konstruktivisme : Jurnal Pendidikan dan Pembelajaran
Dian Puspita Anggraini
Fakultas MIPA Universitas Brawijaya Jalan Veteran Malang 65145
Biochemistry, Genetics & Molecular Biology Education Health Professions Languange, Linguistic, Communication & Media Mathematics Physics Public Health Social Sciences Other

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Determination of Kinetic Parameters and The Effect of Ion Mg2+ Inhibition Into Pectinase Activities. Anggraini, Dian Puspita; Sulistiana, Devita; Agustina, Dwi Kameluh; Ulimaz, Almira
Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika Vol. 4 No. 2 (2020): July
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/e-saintika.v4i2.170

Abstract

The purpose of study was to determine kinetic parameters and inhibitory effect of Mg2+ ions on pectinase. Activity test performed at pH 4,5 temperature 55oC for 50' with 10 mm concentrations of each ion 0-10mm. Galacturonic acid content, as product of pectin substrate hydrolysis, was used as basis for determining activity and analyzed by visible spectrophotometry. At concentrations 2 and 4 mm Mg2+ act as an activator, but at concentrations 6, 8, and 10 mM as inhibitors. The value of KM with and without inhibitors is almost same, that is 0.3145% and 0.3105% but value of Vm from both are different, that is 80,645 μg.ml-1.minute-1 and 62,112 μg.ml-1.minute-1. The conclusion that type of inhibitory pectinase with Mg2+ is a non-competitive inhibition. The inhibition constant value (Ki) is 26,84.
Co-Authors Agustina, Dwi Kameluh Almira Ulimaz Isniawati, Mei Dwi Jauharoh, Sahiba Azzahra Malahayati, Eva Malahayati, Eva Nurul Prayudhi, Luhur Aditya Sholihah, Mar'atus Sodiq, Muhammad Yusuf Fajar Sulistiana, Devita