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Hanandayu Widwiastuti
Poltekkes Kemenkes Malang
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POTENSI ARANG AKTIF DALAM MENURUNKAN KANDUNGAN LOGAM BERAT TIMBAL PADA KERANG KUPANG: POTENSI ARANG AKTIF DALAM MENURUNKAN KANDUNGAN LOGAM BERAT TIMBAL PADA KERANG KUPANG Oktavianti, Elok; Kesuma, Sandry; Widwiastuti, Hanandayu
JURNAL TEKNOLOGI KONSEPTUAL DESAIN Vol 2 No 2 (2025): JTKD- Maret 2025
Publisher : CV.G-Techsolutions

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1980/jurnalteknologikonseptualdesign.v2i2.75

Abstract

Kupang adalah biota laut tergolong mollusca (bertubuh lunak) jenis kerang-kerangan berprotein yang diminati oleh masyarakat, juga termasuk biota laut yang biasa digunakan sebagai indikator pencemaran karena sifatnya yang hidup menetap pada sedimen perairan. Salah satu bahan pencemar pada biota laut yang berbahaya adalah logam berat timbal (Pb). Penelitian ini bertujuan untuk mengetahui pengaruh perebusan dengan penambahan arang aktif terhadap penurunan kadar cemaran logam berat timbal (Pb) pada kupang di Laut Kraton Kabupaten Pasuruan. Penggunaan arang aktif selama perebusan diharapkan dapat mengurangi cemaran logam berat pada kupang. Analisis pengaruh perebusan dengan variasi penambahan arang aktif 0, 5, dan 10 gr menunjukan hasil bahwa kadar logam Pb pada kupang mengalami penurunan setelah dilakukan perebusan dan penambahan arang aktif. Kadar logam Pb pada kupang segar, kupang rebus dengan variasi penambahan arang aktif 0, 5, dan 10 gr masing-masing mengandung logam Pb rata-rata sebesar 0,399 mg/kg, 0,189 mg/kg, 0,083 mg/kg, 0,155 mg/kg. Kadar logam Pb pada kupang yang paling rendah adalah pada variasi penambahan arang aktif 5 gram saat perebusan. Kadar logam Pb pada air sisa rebusan kupang adalah 0,0117 mg/L, pada air sisa rebusan kupang dengan 5 gram arang aktif tidak terdeteksi, dan air sisa rebusan kupang dengan 10 gram arang aktif 0,0031 mg/L. Maka penambahan 5 gram arang aktif lebih efektif dari 10 gram arang aktif
Preparation of Methanyl Yellow Test Strip Based on Nata De Coco Membrane Immobilized Anthocyanin Extracted from Rosella Flower (Hibiscus Sabdariffa L.) Lestari, Yunda; Widwiastuti, Hanandayu
Jurnal Wiyata Penelitian Sains dan Kesehatan Vol 12, No 1 (2025)
Publisher : LP2M IIK (Lembaga Penelitian dan Pengabdian Masyarakat Institut Ilmu Kesehatan) Bhakti Wiy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56710/wiyata.v12i1.757

Abstract

Methanyl yellow is a synthetic dye used in the textile industry and its use is prohibited in food products. Its abuse is still found in crackers, noodles, tofu, and beverages. Methanyl yellow identification test used color reaction test, thin layer chromatography, paper chromatography, TLC scanner, and UV-Vis spectrophotometry. So, in this study, the methanyl yellow test strip test method was developed using anthocyanin immobilized nata de coco membrane with rosella flower extract. The purpose of this study was to determine the selectivity and sensitivity of the resulting test strips. The test strip consisted of nata de coco membrane and rosella flower extract reagent. The test strip made has an optimum contact time of 10 seconds. In the analysis using FTIR, the functional groups O–H, C–H alkanes, C=O, C=C alkenes, C–O, and C–H alkenes on the test strip indicated anthocyanin compounds. In the test strip which was reacted with methanyl yellow the functional group identified had a weaker absorption than the test strip which had not been reacted. The characterization test includes a detection limit test with detection capability starting at a concentration of 6 ppm and lifetime test results, up to the 21st day the strip test can be used, storage using dark bottles is better than clear bottles. So that the resulting test strips provide good data and can be used for quick measurements.
Pemanfaatan Ekstrak Kulit Ubi Jalar Ungu dalam Deteksi Boraks pada Kerupuk Puli yang dijual di Pasar Ngemplak Tulungagung Lauren, Angelita Rajinia; Asworo, Riska Yudhistia; Widwiastuti, Hanandayu
Primary Journal of Multidisciplinary Research Vol. 1 No. 3 (2025): Primary Journal of Multidisciplinary Research, June 2025
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/pjmr.v1i3.210

Abstract

Method validation is one of the quality assurance processes that must be carried out on a developed Method Anthocyanins in purple sweet potato peel can be used as a reagent in a test kit for method development to detect borax. Borax is a substance prohibited as a food additive, yet it is still commonly found in food products such as kerupuk puli (a type of Indonesian cracker). Therefore, it is necessary to validate the test kit method using purple sweet potato peel extract to determine the borax content in kerupuk puli sold at Ngemplak Market, Tulungagung. The research method used was experimental, involving organoleptic testing, creation of a color comparator, method validation, and both qualitative and quantitative testing of borax in kerupuk puli. Organoleptic results from 5 samples indicated the possible presence of borax, characterized by a crispy texture and a bitter taste. The color comparator produced showed that purple sweet potato peel extract can detect borax, indicated by different color changes corresponding to each variation in borax concentration. The method validation results met the required criteria, including linearity with an R² of 0.996; Limit of Detection (LoD) of 0.028%; Limit of Quantification (LoQ) of 0.095%; accuracy of 101.04%; and Relative Standard Deviation (RSD) of 1.8%. The kerupuk puli samples tested using this method showed the presence of borax at a concentration of 0.0002%.
Preparation of Methanyl Yellow Test Strip Based on Nata De Coco Membrane Immobilized Anthocyanin Extracted from Rosella Flower (Hibiscus Sabdariffa L.) Lestari, Yunda; Widwiastuti, Hanandayu
Jurnal Wiyata Penelitian Sains dan Kesehatan Vol 12 No 1 (2025)
Publisher : LP2M IIK (Lembaga Penelitian dan Pengabdian Masyarakat Institut Ilmu Kesehatan) Bhakti Wiy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56710/wiyata.v12i1.757

Abstract

Methanyl yellow is a synthetic dye used in the textile industry and its use is prohibited in food products. Its abuse is still found in crackers, noodles, tofu, and beverages. Methanyl yellow identification test used color reaction test, thin layer chromatography, paper chromatography, TLC scanner, and UV-Vis spectrophotometry. So, in this study, the methanyl yellow test strip test method was developed using anthocyanin immobilized nata de coco membrane with rosella flower extract. The purpose of this study was to determine the selectivity and sensitivity of the resulting test strips. The test strip consisted of nata de coco membrane and rosella flower extract reagent. The test strip made has an optimum contact time of 10 seconds. In the analysis using FTIR, the functional groups O–H, C–H alkanes, C=O, C=C alkenes, C–O, and C–H alkenes on the test strip indicated anthocyanin compounds. In the test strip which was reacted with methanyl yellow the functional group identified had a weaker absorption than the test strip which had not been reacted. The characterization test includes a detection limit test with detection capability starting at a concentration of 6 ppm and lifetime test results, up to the 21st day the strip test can be used, storage using dark bottles is better than clear bottles. So that the resulting test strips provide good data and can be used for quick measurements.
Sintesis Nanopartikel Perak Dengan Menggunakan Bioreduktor Ekstrak Kulit Buah Sirsak (Annora muricate L.) Untuk Identifikasi Logam Berat Merkuri pada Whitening Body Lotion yang Dijual di E-Commerce Riska Yudhistiia Asworo; Raheny, Nafrila; Widwiastuti, Hanandayu; Sabila, Nurma
JURNAL ILMIAH FARMASI SIMPLISIA Vol. 5 No. 2 (2025): Desember 2025
Publisher : Jurusan Farmasi, Politeknik Kesehatan Kementerian Kesehatan Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/jifs.v5i2.896

Abstract

Penggunaan merkuri sebagai pemutih masih banyak ditemukan pada whitening body lotion yang tidak memiliki izin edar. Identifikasi merkuri dapat dilakukan secara kolorimetri menggunakan Nanopartikel Perak (AgNPs). AgNPs dapat disintesis dengan menggunakan bioreduktor dari metabolit sekunder yang ada pada ekstrak kulit buah sirsak. Pada pembentukan AgNPs perbandingan volume mempengaruhi hasil nanopartikel yang terbentuk. Variasi perbandingan volume yang digunakan pada penelitian ini (ekstrak kulit buah sirsak: larutan AgNO3 2 mM) antara lain 1:20; 1:30; dan 1:40 (v:v). Karakterisasi AgNPs yang terbentuk pada berbagai variasi komposisi dilakukan menggunakan Spektrofotometer UV-Vis.  Nilai panjang gelombang maksimum 400-450 nm menandakan AgNPs telah terbentuk. Hasil  AgNPs dengan absorbansi optimum yang didapatkan selanjutnya dikarakterisasi menggunakan Particle Size Analyzer (PSA). AgNPs pada kondisi optimum  digunakan untuk identifikasi merkuri pada  sampel whitening body lotion. Dari penelitian ini didapatkan semua variasi komposisi AgNPs dapat terbentuk AgNPs dengan komposisi paling optimum pada 1:20 dengan ukuran partikel 77,12 nm. Pengujian sampel secara kolorimetri dengan AgNPs menunjukkan 1 dari 5 sampel positif merkuri. Sampel positif pada sampel E dengan adanya perubahan warna dari cokelat menjadi putih keruh, semakin lama memudar menjadi tidak berwarna. Uji pembanding dengan menggunakan kalium iodida juga menunjukkan hasil positif  merkuri pada sampel E.
Co-Authors Abriyanti Dewi Akhmad Sabarudin Ani Mulyasuryani Aswaro, Riska Yudistia Dewi, Abriyanti Elok Widayanti Hanun, I’sta Suraya Indah Cahyaning Wulan Lauren, Angelita Rajinia Liffany, Shelsabila Dhea Niken Cahyaning Wulandari Nurma Sabila Oktavianti, Elok Permatasari, Silvi Diaz Raheny, Nafrila Rany Adelina Riska Yudhistia Asworo Riska Yudhistiia Asworo Sandry Kesuma Wulandari, Niken Cahyaning Yudhistia Asworo, Riska Yunda Lestari, Yunda Yustina Suhandini Tjahjaningsih