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The Effectiveness o f Entomopathogenic Fungi Beauveria bassiana with the Addition of Insect Growth Regulator Lufenuron f or Controlling Bactrocera carambolae Hadi, Mochammad Syamsul; Himawan, Toto; Aini, Luqman Qurata
Journal of Tropical Life Science Vol 3, No 3 (2013)
Publisher : Journal of Tropical Life Science

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The study of the effectiveness of entomopathogenic fungus Beauveria bassiana (Bals) Vuill. with the addition of an insect growth regulator (IGR) lufenuron to control the fruit fly Bactrocera carambolae (Drew and Hancock) (Diptera: Tephritidae) was conducted at the Laboratory of Toxicology and Laboratory of Entomology, Department of Plant Pests and Diseases, Faculty of Agriculture, University of Brawijaya Malang from March 2012 until December 2012. This study examined the effect of lufe-nuron to the development and sporulation of B. bassiana and the effectiveness of the fungus B. bassiana in combination with several concentrations of lufenuron (0.5, 1, 1.5 mL/L) on the mortality of fruit fly larvaB. carambolae. The results showed that the addition of lufenuron at the concentration of 1 mL/L could significantly increase the sporulation of B. bassiana. The addition of B. bassiana at 108 spores/mL combined with lufenuron at the concentration of 1 mL/L applied to compost pupation medium of larva of B. carambolae is significantly able to suppress the formation of the fruit fly pupa and imago of B. carambolae

Pathogenicity of Entomophatogenic Fungi Lecanicillium lecanii Against Predator Insect Menochilus Sexmaculatus Hadi, Mochammad Syamsul; Taufiqurrahman, Achmad Fitriadi; Choliq, Fery Abdul; Istiqomah, Istiqomah; Karindah, Sri
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 2 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.115.63-68

Lecanicillium lecanii is an insect pathogenic fungus that is often used for pest control and has a wide range of hosts. The L. lecanii is capable of infecting several types of host insects including the Order Orthoptera, Hemiptera, Lepidoptera, Thysanoptera and Coleoptera. The extent of this fungus host range was feared to have a negative effect on predator insects Menochilus sexmaculatus. This study aims were to determined the pathogenicity of the fungus L. lecanii against the imago predatory beetle M. sexmaculatus, to know how the predation ability and the number of eggs fecundity of Imago M. sexmaculatus after application L. lecanii. The research was arranged in Randomized Block Design with 4 replications. The conidia density of L. lecanii used were 106, 107, 108, 109 conidia/ml, 1 ml / l of lufenuron insecticide as positive control and sterile distilled water as negative control. The research showed that the mortality percentage of M. sexmaculatus due to L. lecanii application is low and medium. The L. lecanii was not affected for preying ability on imago M. sexmaculatus but gave effect to the number of eggs fecundity of imago M. sexmaculatus.

Pathogenicity of Entomophatogenic Fungi Lecanicillium lecanii Against Predator Insect Menochilus Sexmaculatus Hadi, Mochammad Syamsul; Taufiqurrahman, Achmad Fitriadi; Choliq, Fery Abdul; Istiqomah, Istiqomah; Karindah, Sri
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 2 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.115.63-68

Lecanicillium lecanii is an insect pathogenic fungus that is often used for pest control and has a wide range of hosts. The L. lecanii is capable of infecting several types of host insects including the Order Orthoptera, Hemiptera, Lepidoptera, Thysanoptera and Coleoptera. The extent of this fungus host range was feared to have a negative effect on predator insects Menochilus sexmaculatus. This study aims were to determined the pathogenicity of the fungus L. lecanii against the imago predatory beetle M. sexmaculatus, to know how the predation ability and the number of eggs fecundity of Imago M. sexmaculatus after application L. lecanii. The research was arranged in Randomized Block Design with 4 replications. The conidia density of L. lecanii used were 106, 107, 108, 109 conidia/ml, 1 ml / l of lufenuron insecticide as positive control and sterile distilled water as negative control. The research showed that the mortality percentage of M. sexmaculatus due to L. lecanii application is low and medium. The L. lecanii was not affected for preying ability on imago M. sexmaculatus but gave effect to the number of eggs fecundity of imago M. sexmaculatus.

The Effectiveness of Three Strains of Beauveria bassiana (Balsamo) Vuillemin Isolate with Different Densities and Tobacco Leaf Extract on Plutella xylostella Linnaeus (Lepidoptera: Plutellidae) Hadi, Mochammad Syamsul; Siringoringo, Intan; Widjayanti, Tita; Setiawan, Yogo
Plantropica: Journal of Agricultural Science Vol. 8 No. 1 (2023)
Publisher : Department of Agronomy, Faculty of Agriculture, Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/10.21776/ub.jpt.2023.008.1.9

This study aims to determine the pathogenicity and feeding inhibition of three B. bassiana isolate strains with different densities and concentrations of EDT (Tobacco Leaf Extract) in controlling P. xylostella. Research conducted in the Lab. Brenjonk Biological Agency and Lab. BPTPH Pasuruan from October to December 2022. This study used a Randomized Block Design (RAK) to examine the mortality and feeding inhibition of B. basiana and EDT strain isolates against P. xylostella. And probit analysis to find out the LC50 value and LT50 value. In this study, there were 17 treatments consisting of 15 larvae in each treatment and 4 repetitions were carried out. The results showed that the highest mortality percentage of P. xylostella was found in B. bassiana isolates of Bojonegoro and Jatisari strains at densities of B. bassiana 108 and 1010. The lowest LC50 value of B. bassiana isolates was the Bojonegoro strain and the EDT LC50 value was 43.79 ml/lt. Meanwhile, the lowest LT50 value was found in the EDT treatment at 28.40 hours. Based on the test results, it was also known that B. bassiana and EDT had potent feeding inhibition against P. xylostella. However, the use of EDT in controlling P. xylostella was less effective and efficient than the application of B. bassiana.

Exploration of Symbiont Bacteria Potentially Degrading Chlorpyrifos Insecticide in the Digestive Tract of Beet Armyworm (Spodoptera exigua) in Kediri Regency Widjayanti, Tita; Hadi, Mochammad Syamsul; Tantri, Zalfa Meiska
Plantropica: Journal of Agricultural Science Vol. 8 No. 2 (2023)
Publisher : Department of Agronomy, Faculty of Agriculture, Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpt.2023.008.2.10

Spodoptera exigua Hubner (Lepidoptera: Noctuidae) is an important pest on shallot crops and cosmopolitan with a fast life cycle. In controlling S. exigua farmers use chemical insecticides such as chlorpyrifos. Intensive application of insecticides causes resistance of S. exigua. Resistance can occur due to several factors, one of which is biological factors. In biological factors, there is a symbiosis between pests and beneficial bacteria called symbiont bacteria. It is suspected that symbiont bacteria symbiotic with S. exigua can degrade chlorpyrifos. The research was conducted from December 2022 to May 2023. The research began with a site survey and sampling in three sub-districts in Kediri Regency, namely Plemahan District, Kepung District, and Badas District. Furthermore, samples of S. exigua were studied at the Pesticide Toxicology Laboratory, Brawijaya University. The research conducted includes (1) exploration, isolation, and purification of symbiont bacteria in the digestive tract of S. exigua, (2) bioassay test of symbiont bacteria that have the potential to degrade chlorpyrifos, and (3) characterization of bacteria that have the potential to degrade chlorpyrifos. The results of the exploration of symbiont bacteria in the digestive tract of S. exigua obtained as many as 45 isolates of bacteria capable of growing in NA + chlorpyrifos media. Furthermore, from bioassay testing, 14 isolates of bacteria that have the potential to degrade chlorpyrifos insects. The characterization results showed that bacterial isolates that have the potential to degrade chlorpyrifos insecticides come from the genera Pantoea, Erwinia, and Coryneform.

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