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All Journal Reaktor Eksergi: Chemical Engineering Journal Bulletin of Chemical Reaction Engineering & Catalysis Journal of Mathematical and Fundamental Sciences Journal of Engineering and Technological Sciences JURNAL SELULOSA Journal of Industrial and Information Technology in Agriculture ASEAN Journal of Chemical Engineering Jurnal Teknik Kimia Indonesia Jurnal Riset Teknologi Pencegahan Pencemaran Industri Bulletin of Chemical Reaction Engineering & Catalysis

Setiadi, Tjandra

Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Bandung, Jalan Ganesa No. 10 Bandung 40132,

Author-ID : 444259

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Physics

Published : 32 Documents Claim Missing Document

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PRODUKSI LAKASE DAN POTENSI APLIKASINYA DALAM PROSES PEMUTIHAN PULP (LACASE PRODUCTION AND ITS POTENTIAL IN PULP BLEACHING PROCESS) Hendro Risdianto; Sri H. Suhardi; Wardono Niloperbowo; Tjandra Setiadi
JURNAL SELULOSA Vol 43, No 01 (2008): BERITA SELULOSA
Publisher : Center for Pulp and Paper

This study was focused on production and utilisation of laccase in pulp bleaching process. Laccase was produced by white-rot fungi of Marasmius sp which was immobilised in luffa sponge in a modified immersion bioreactor. The Experiment was conducted to study the effect of variation of immersion periods in the production of laccase. The immersion periods applied in this study were 15 minutes, 12 hours and 24 hour respectively. Lacase was then utilised in pre-treatment of pulp bleaching of Accacia mangium unbleached-pulp. The bleaching process were conducted with and without addition mediator of 2,2'-azinobis 3-ethylbenzthiazoline-6-sulphonic acid (ABTS) which has ability to enhance the delignification process.The results showed that immersion period of 12 hours exhibited the highest maximum laccase activity. Maximum level of laccase attained from culture with 12 hours immersion period was 457.6 U/L. When the immersion period be double ( 24 hours), the level of laccase, however, becoming lower i.e. 281.9 U/L. A 15 minutes immersion period has resulted in a level of laccase of 384.4 U/L. Utilisation of crude enzymes by addition of ABTS mediator with enzyme exposure time 6 hours at temperature of 45oC has increased brightness for 2.8 points. Pretreatment in similar conditions with no ABTS has added showed an increase in brightness for 0.7 point. It demonstrated that the addition of ABTS has improved biobleaching process. The pulp exposed to crude enzymes for 6 days at room temperature has increased the brightness of pulp to 5.3 points. These results indicate that laccase has a potential ability in pulp bleaching process.Keywords: a modified temporary immersion bioreactor, bleaching, brightness\, crude enzyme, laccase, luffa, Marasmius sp., white-rot fungi.INTISARIPenelitian ini ditujukan untuk memproduksi lakase dan menggunakannya dalam proses pemutihan pulp. Produksi lakase dilakukan dengan menggunakan jamur pelapuk putih Marasmius sp. yang diimobilisasi pada bulustru. Bioreaktor yang digunakan adalah bioreaktor imersi berkala termodifikasi. Penelitian produksi lakase dilakukan dengan variasi waktu imersi 15 menit, 12 jam dan 24 jam. Produk berupa ekstrak kasar lakase digunakan sebagai perlakuan awal proses pemutihan pulp Acasia mangium. Pemutihan pulp dilaksanakan dengan dan tanpa mediator 2,2'-azinobis 3-ethylbenzthiazoline-6-sulphonic acid (ABTS) yang berfungsi untuk meningkatkan proses delignifikasi.Hasil penelitian menunjukkan bahwa waktu immersi 12 jam menghasilkan lakase dengan aktivitas paling tinggi yaitu mencapai 457,6 U/L, lebih tinggi dibandingkan waktu immersi 15 menit (348,4 U/L) dan waktu imersi 24 jam (281,9 U/L). Pemutihan pulp menggunakan ekstrak kasar lakase dengan bantuan ABTS selama 6 jam dan suhu 45oC dapat meningkatkan derajat putih sebesar 2,8 poin. Sedangkan pada kondisi yang sama namun tanpa penambahan ABTS hanya dapat meningkatkan derajat putih 0,7 poin. Hal ini menunjukkan bahwa penggunaan ABTS dapat meningkatkan proses pemutihan. Sementara penggunaan ekstrak kasar lakase selama enam hari dapat meningkatkan derajat putih sebesar 5,3 poin. Dengan demikian, lakase memiliki potensi untuk peningkatan derajat putih proses pemutihan pulp yang diharapkan dapat mengurangi kebutuhan bahan kimia pemutih dan mengurangi limbah berbahaya proses pemutihan.Kata kunci: bioreaktor imersi berkala termodifikasi, pemutihan, derajat putih, ekstrak kasar, lakase, bulustru, Marasmus sp., jamur pelapuk putih.

OPTIMISASI PRODUKSI ENZIM LAKASE PADA FERMENTASI KULTUR PADAT MENGGUNAKAN JAMUR PELAPUK PUTIH Marasmius sp. : PENGARUH UKURAN PARTIKEL, KELEMBAPAN, DAN KONSENTRASI Cu Cornelius Damar Hanung; Ronald Osmond; Hendro Risdianto; Sri Harjati Suhardi; Tjandra Setiadi
JURNAL SELULOSA Vol 3, No 02 (2013): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

White rot fungi of Marasmius sp. is a fungus which produce laccase in high activity. Laccase is one of the ligninolityc enzymes that capable to degrade lignin. This ability can be used for the pretreatment of lignocellulosic materials in the bioethanol production. Laccase was produced in flask by batch process using Solid State Fermentation (SSF). The optimisation was conducted by statistically of full factorial design. The particle size, moisture content, and Cu concentration were investigated in this study. Rice straw was used as solid substrate and the glycerol was used as the carbon sources in modified Kirk medium. The results showed that particle size of rice straw did not affect significantly to the enzyme activity. The highest laccase activity of 4.45 IU/g dry weight was obtained at the moisture content of 61% and Cu concentration of 0.1 mM.Keywords: laccase, Marasmius sp., optimisation, rice straw, solid state fermentation ABSTRAKJamur pelapuk putih, Marasmius sp. merupakan jamur yang menghasilkan enzim lakase dengan aktivitas tinggi. Lakase merupakan enzim ligninolitik yang dapat mendegradasi lignin. Kemampuan ini dapat digunakan untuk proses pengolahan awal bahan lignoselulosa pada pembuatan bioetanol. Produksi lakase dilakukan dalam labu dengan modus batch menggunakan fermentasi kultur padat. Optimisasi produksi enzim lakase dengan metode fermentasi padat dilakukan dengan rancangan percobaan faktorial penuh. Pengaruh ukuran partikel, kelembapan, dan konsentrasi Cu diuji dengan medium penyangga jerami dengan menambahkan gliserol dalam medium Kirk termodifikasi sebagai sumber karbon. Penelitian ini menunjukkan bahwa ukuran jerami tidak berpengaruh signifikan terhadap aktivitas enzim. Aktivitas enzim lakase maksimum terjadi pada saat kelembapan 61% dan konsentrasi Cu 0,1 mM dengan aktivitas enzim lakase/berat kering tertinggi mencapai 4,45 IU/g.Kata kunci: lakase, Marasmius sp., optimisasi, jerami, fermentasi kultur padat

Pengaruh Sumber Karbon pada Produksi Lakase dari Jamur Pelapuk Putih Marasmius sp. dalam Fermentasi Kultur Padat Hendro Risdianto; Elis Sofianti; Suraya Suraya; Sri Harjati Suhardi; Tjandra Setiadi
JURNAL SELULOSA Vol 8, No 02 (2018): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

Lakase merupakan salah satu enzim ligninolitik yang memiliki kemampuan mendegradasi lignin. Lakase telah diproduksi menggunakan jamur pelapuk putih Marasmius sp. dalam Fermentasi Kultur Padat (FKP) menggunakan jerami padi sebagai media pertumbuhan. Pengaruh sumber karbon yaitu glukosa, gliserol, dan molase dalam medium produksi lakase digunakan dalam penelitian ini. Konsentrasi 0,5%; 1,0%; dan 2,0% digunakan untuk tiap jenis sumber karbon. Hasil menunjukkan bahwa aktivitas tertinggi lakase diperoleh pada kultivasi hari ke 6-10 dengan masing-masing aktivitas (872,0 U/L (hari ke-6), 1516,67 U/L (hari ke-9) dan 1270,69 U/L (hari ke-10). Aktivitas lakase tertinggi diperoleh pada penggunaan medium gliserol dan molase masing-masing adalah 1422,36 U/L (pada konsentrasi 1%, hari ke-7) dan 113,19 U/L (pada konsentrasi 2%, hari ke-8). Aktivitas tertinggi tersebut sebanding dengan penggunaan medium glukosa. Oleh karena itu, gliserol dan molase dapat digunakan sebagai alternatif sumber karbon untuk produksi lakase dengan fermentasi kultur padat.Kata kunci: glukosa, gliserol, lakase, molase, Marasmius sp., fermentasi kultur padat Influence of Carbon Sources on Laccase Production by White Rot Fungus Marasmius sp. in Solid State FermentationAbstractLaccase is an one of the ligninolytic enzymes that capable to degrade lignin in biomass. Laccase has been produced by white rot fungus Marasmius sp. in Solid State Fermentation (SSF) using rice straw as the solid support media. The influence of carbon sources, i.e. glucose, glycerol and molasses in medium of laccase production were studied in this paper. The concentration of 0.5%, 1.0% and 2.0% were used for each carbon sources. The results showed that the highest lacase activity was obtained within 6-10 days of cultivation. Glucose concentration of 0.5%, 1.0% and 2.0% gave the highest laccase activity were 872.0 U/L (day 6), 1516.67 U/L (day 9) and 1270.69 U/L (day 10) respectively. The highest laccase activity on using glycerol and molasses was 1422.36 U/L (at concentration of 1 % on day 7th) and 1113.19 U/L (at concentration of 2% on day 8th), respectively. This activity was comparable to that of glucose substrate. Therefore, glycerol and molasses gave a potential chance as carbon sources for the strategy on low cost laccase production in solid state fermentation.Keywords: glucose, glycerol, laccase, molasses, Marasmius sp., solid state fermentation.

Identifikasi Senyawa Asam Lemak Volatil dari Air Limbah Industri Minyak Kelapa Sawit untuk Produksi Polihidroksialkanoat oleh Ralstonia eutropha JMP 134 Martha Aznury; Tjandra Setiadi
JURNAL SELULOSA Vol 11, No 01 (2021): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25269/jsel.v11i01.340

Identification of Volatile Fatty Acids from Palm Oil Mill Effluent for Polyhydroxyalkanoate Production by Ralstonia eutropha JMP 134 Abstract Polyhydroxyalkanoate (PHA) is a bioplastic which is derived from bacterial fermentation. In this study, PHA is produced by utilizing Ralstonia eutropha JMP 134 and volatile fatty acids (VFA) from palm oil industrial wastewater as a precursor. The aim of this research is to study the effect of carbon source, addition time, and VFA concentration on PHA production by fermentation using Ralstonia eutropha JMP 134 in batch. PHA and dry cell weight (DCW) concentrations obtained by adding VFA from palm oil industrial wastewater in batches at 20 and 40 hours were 0.014 g/L.hour, 2.76 g/L and 3.66 g/L, respectively. The results also showed that the time of adding VFA greatly affected cell growth, with the best addition time being after the 20th hour. Keywords: palm oil industrial wastewater, polyhydroxyalkanoate (PHA), batch, Ralstonia eutropha JMP 134, volatile fatty acids (VFA) Abstrak Polihidroksialkanoat (PHA) adalah bioplastik yang diproses melalui proses fermentasi dengan mikroba. Pada penelitian ini, PHA diproduksi dengan menggunakan Ralstonia eutropha JMP 134 dan memanfaatkan asam lemak volatil (ALV) dari air limbah industri minyak kelapa sawit sebagai prekursor. Penelitian ditujukan mempelajari pengaruh sumber karbon, waktu penambahan, dan konsentrasi ALV terhadap poduksi PHA yang difermentasi menggunakan Ralstonia eutropha JMP 134 secara batch. Konsentrasi PHA dan berat kering sel (BKS) yang diperoleh pada penambahan ALV dari air limbah industri kelapa sawit secara batch pada jam ke-20 dan 40 masing-masing bernilai 0,014 g/L.jam, 2,76 g/L dan 3,66 g/L. Hasil penelitian memperlihatkan pula bahwa waktu penambahan ALV sangat mempengaruhi pertumbuhan sel, dengan waktu penambahan yang terbaik adalah setelah pada jam ke-20. Kata kunci: air limbah industri minyak kelapa sawit, polihidroksialkanoat (PHA), batch, Ralstonia eutropha JMP 134, asam lemak volatil (ALV)

Co-Authors Abdul Aziz Abdul Haris Mulyadi Achmad Ali Syamsuriputra Adi Pancoro Agus Jatnika Effendi Al-Aziz, Rd. Habib R. M. T. Andi Trirakhmadi Ardiyan Harimawan Awalina Satya Bambang Veriansyah Bork Ilsemann Choerudin, Choerudin Cornelius Damar Hanung D Desiana Damir Brdjanovic Damir Brdjanovic David Rusdi Dennis Farina Nury Devianto, Hary Dewinka Anugeraheni Diah Meilany Diah Meilany Dian Shofinita Efri Mardawati Elis Sofianti Elis Sofianti Elis Sofianti ESMIRALDA Esmiralda EVELYN EVELYN Gadis Sri Haryani H Haryono Harimawan, Ardiyan Hendro Risdianto Hendro Risdianto Hendro Risdianto Hendro Risdianto Hendro Risdianto Hendro Risdianto Herriyanto Ronny Sondjaja I G B N Makertihartha I.G.B Ngurah Makertihartha Ibnu Maulana Hidayatullah Ilsemann, Bork Indrie Ratnasari Istiqomah, Noviani Arifina Khairul Hadi Burhan M.T.A.P. Kresnowati Made Tri Ari Penia Kresnowati Made Tri Ari Penia Kresnowati Martha Aznury Martha Aznury Mike Reich Mindriany Syafila Muhammad Zulfikar Luthfi Mukti, Rendy Prayatni Soewondo Prayatni Soewondo Pusparizkita, Yustina Metanoia Rd. Habib R. M. T. Al-Aziz Reich, Mike Ronald Osmond Rusdi, David Satya, Awalina Schmahl, Wolfgang Shofinita, Dian Sri H. Suhardi Sri Harjati Suhardi Sri Harjati Suhardi Sri Harjati Suhardi Sri Harjati Suhardi Sri Haryani, Gadis Suhendri Suhendri Suraya Suraya Tan Mellisa Tantra Tantra, Tan Mellisa Thye Yoke Pean Thye Yoke Pean Trimaharika Widarena Wardono Niloperbowo Wita Dewi Sondari Wolfgang Schmahl Yustina Metanoia Pusparizkita

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