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All Journal Reaktor Eksergi: Chemical Engineering Journal Journal of Mathematical and Fundamental Sciences Journal of Engineering and Technological Sciences JURNAL SELULOSA Journal of Industrial and Information Technology in Agriculture ASEAN Journal of Chemical Engineering Jurnal Teknik Kimia Indonesia Jurnal Riset Teknologi Pencegahan Pencemaran Industri Bulletin of Chemical Reaction Engineering & Catalysis

Setiadi, Tjandra

Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Bandung, Jalan Ganesa No. 10 Bandung 40132,

Author-ID : 444259

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Physics

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KINETIC STUDY OF OIL PALM EMPTY FRUIT BUNCH ENZYMATIC HYDROLYSIS Diah Meilany; Efri Mardawati; Made Tri Ari Penia Kresnowati; Tjandra Setiadi
Reaktor Volume 17 No. 4 Desember 2017
Publisher : Dept. of Chemical Engineering, Diponegoro University

As lignocellulosic biomass, Oil Palm Empty Fruit Bunch (OPEFB) can be used as the source of xylose that can be further utilized as the raw material for xylitol production. The processing of OPEFB to xylose comprises of pretreatment and hydrolysis that can be performed enzymatically. This process offers the advantages of moderate operation conditions and more environmentally friendly. This article describes the kinetic study of enzymatic hydrolysis process of OPEFB for producing xylose using self-prepared and commercial xylanase enzymes. Despite the possible mass transfer limitation, the Michaelis Menten kinetics was hypothesized. The results indicated that the reaction at pH 5 and 60°C followed the Michaelis Menten kinetics, with Vm of 0.84 g/L-h and Km of 48.5 g/L for the commercial enzyme, and Vm of 0,38 g/L-h and Km of 0,37 g/L for the self-prepared enzyme. The reaction is affected by temperature, with Ea of 8.6 kcal/gmol. The performance of self-prepared xylanase enzyme was not yet as good as the commercial enzyme, Cellic Htec 2. Keywords: enzymatic hydrolysis; kinetics parameter; OPEFB; xylanase; xylose

The Effects of Operational Conditions in Scaling Up of Xylanase Enzyme Production for Xylitol Production Diah Meilany; Dewinka Anugeraheni; Abdul Aziz; Made Tri Ari Penia Kresnowati; Tjandra Setiadi
Reaktor Volume 20 No.1 March 2020
Publisher : Dept. of Chemical Engineering, Diponegoro University

The biological route to produce xylitol from Oil Palm Empty Fruit Bunches (EFBs) comprises of EFBs pretreatment, enzymatic hydrolysis, fermentation, and downstream separation of the produced xylitol. Due to the specificity in the hemicellulose composition of EFBs, a xylanase enzyme that has a high affinity to EFBs is required to hydrolyze the EFBs into xylose. In this research, the influences of aeration, humidity, and mixing in xylanase production were mapped. The xylanase production was performed by Aspergillus fumigatus ITBCCL170 in a solid-state fermentation using a tray fermenter with EFBs as the substrate. The optimal configuration was further scaled up into xylanase production using 1000 g of EFBs as the substrate. The results showed that the highest enzyme activity was 236.3 U/g EFB, obtained from the use of humid air airflow of 0.1 LPM, and mixing was performed once a day. The scaling up resulted in a lower xylanase activity and call for a better design of the fermenter.Keywords: aeration, humidity, mixing, OPEFBs, tray fermenter, xylanase, xylitol

Co-Authors Abdul Aziz Abdul Haris Mulyadi Achmad Ali Syamsuriputra Adi Pancoro Agus Jatnika Effendi Al-Aziz, Rd. Habib R. M. T. Andi Trirakhmadi Awalina Satya Bambang Veriansyah Bork Ilsemann Choerudin, Choerudin Cornelius Damar Hanung D Desiana Damir Brdjanovic Damir Brdjanovic David Rusdi Dennis Farina Nury Dewinka Anugeraheni Diah Meilany Diah Meilany Dian Shofinita Efri Mardawati Elis Sofianti Elis Sofianti Elis Sofianti ESMIRALDA Esmiralda EVELYN EVELYN Gadis Sri Haryani H Haryono Harimawan, Ardiyan Hary Devianto Hendro Risdianto Hendro Risdianto Hendro Risdianto Hendro Risdianto Hendro Risdianto Hendro Risdianto Herriyanto Ronny Sondjaja I G B N Makertihartha I.G.B Ngurah Makertihartha Ibnu Maulana Hidayatullah Ilsemann, Bork Indrie Ratnasari Istiqomah, Noviani Arifina Khairul Hadi Burhan M.T.A.P. Kresnowati Made Tri Ari Penia Kresnowati Made Tri Ari Penia Kresnowati Martha Aznury Martha Aznury Mike Reich Mindriany Syafila Muhammad Zulfikar Luthfi Mukti, Rendy Prayatni Soewondo Prayatni Soewondo Pusparizkita, Yustina Metanoia Rd. Habib R. M. T. Al-Aziz Reich, Mike Ronald Osmond Rusdi, David Satya, Awalina Schmahl, Wolfgang Shofinita, Dian Sri H. Suhardi Sri Harjati Suhardi Sri Harjati Suhardi Sri Harjati Suhardi Sri Harjati Suhardi Sri Haryani, Gadis Suhendri Suhendri Suraya Suraya Tan Mellisa Tantra Tantra, Tan Mellisa Thye Yoke Pean Thye Yoke Pean Trimaharika Widarena Wardono Niloperbowo Wita Dewi Sondari Wolfgang Schmahl Yustina Metanoia Pusparizkita

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