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All Journal Journal of Dentistry Indonesia ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Scientific Dental Journal Makara Journal of Health Research Sriwijaya Journal of Dentistry Odonto dental journal
Erik Idrus
Department Of Oral Biology, Faculty Of Dentistry, Universitas Indonesia, Indonesia
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Journal : Scientific Dental Journal

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Strawberry Extract’s Effects on Enterococcus faecalis and Porphyromonas gingivalis Biofilms in vitro Armelia Sari Widyarman; Stephanie Brigitta Widjaja; Erik Idrus
Scientific Dental Journal Vol. 1 No. 1 (2017): September
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v1i1.1911

Abstract

Background: Enterococcus faecalis (E. faecalis) and Porphyromonas gingivalis (P. gingivalis) are oral bacteria related to root canal infection and periodontal disease pathogenesis. Strawberries (Fragaria x ananassa) fruit are rich in vitamins and minerals, have antibacterial and antioxidant effects. Objective: This study investigated the inhibition effect of strawberry extract on monospecies and multispecies E. faecalis and P. gingivalis bacteria grown as biofilms in vitro. Methods: This study used E. faecalis ATCC 29212 and P. gingivalis ATCC 33277. It analyzed the effect of strawberry extract on bacteria biofilm formation using a biofilm assay on microplate wells. Five concentrations of strawberry extracts were used (100%, 50%, 25%, 12.5%, and 6.25%), and the inhibition effect was observed after a 1h, 3h, 6h, and 24h incubation period. Biofilms without the strawberry extract were used as the negative controls, and crystal violet and safranin (0.5%w/v) were used to count the biofilm mass. The biofilms grown on microplates were counted using an ELISA reader at 450 nm after 200 mL of 90% ethanol was added to attract the absorbed stain. The strawberry extract inhibition effectiveness on the biofilm formation of each bacterium tested was analyzed using one-way Anova, where p<0.05 was defined as a significant difference. Result: The strawberry extract inhibited the tested monospecies and multispecies bacteria biofilm formation. The optimal strawberry extract concentration for the inhibition of either monospecies biofilms was 100%. However, the optimal incubation time for the strawberry extract to inhibit the multispecies biofilm formation was 24h, which was the study’s biofilm maturity phase. Conclusions: The 100% strawberry extract concentration inhibited the formation of both the monospecies and multispecies E. faecalis and P. gingivalis biofilms. Future studies are needed to evaluate the potential of strawberry extract as an alternative dental therapy.
Co-Authors Amir, Lisa Rinanda Anton Rahardjo Ardiman, Karenina Raihani Amalia Bramma Kiswanjaya Cunningham, Paul J. Dewi Fatma Suniarti Endang Winiati Bachtiar Hartanti, Pratiwi Dwi Hartanto, Willy Hery Widijanto Julie Nicholson LESTARI, WIDYA Lestari, Widya Lubis, Hilda Fitria Maharani, Diah A. Mandasari, Masita Prasetyo, Sri Redjeki Sammy Fajar Nugraha Schäfer, Fred Sri Angky Soekanto Sri Angky Soekanto Stephanie Brigitta Widjaja Subarnbhesaj, Ajiravudh Widyarman, Armelia Sari Yuniardini Septorini Wimardhani