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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Buletin Peternakan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu dan Teknologi Hasil Ternak Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Animal Production : Indonesian Journal of Animal Production Akta Agrosia Jurnal Teknologi Hasil Peternakan Jurnal Agripet Animal Production
Cahyo Budiman
Department Of Animal Production And Technology, Faculty Of Animal Science, Institut Pertanian Bogor, Bogor, 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Veterinary Other

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Structural Homology Modelling, Molecular Docking and Initial Insight into Phenol Degradation Activity of Recombinant Tyrosinase from Lentinula edodes Mushroom: Structure and Phenol Decontamination by Lentinula edodes Tyrosinase David, Viaevanilly; Abdul Rasah, Mohd Nasir; Lindang, Herman Umbau; Mokhtar, Ruzaidi Azli Mohd; Seelan, Jaya Seelan Sathiya; Razali, Rafida; Budiman, Cahyo
Journal of Tropical Life Science Vol. 16 No. 01 (2026)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.16.01.05

Abstract

Tyrosinase is a copper-containing enzyme known for ability to degrade phenolic compounds, making it highly attractive for industrial applications, particularly in wastewater decontamination. While fungal tyrosinase is considered an excellent source for enzyme studies and applications, deeper investigations into its structural properties and phenol degradation activity are still needed. This study aims to elucidate the structural properties of tyrosinase from the mushroom Lentinula edodes (Edo-Tyr) and evaluate its phenol degradation activity. The amino acid sequence of Edo-Tyr was retrieved from GenBank (AB 033993.1) and used to construct three-dimensional models via Robetta, SWISS-MODEL, and Phyre2. The best model obtained from SWISS-MODEL indicated that the structure is predominantly helical, with a Cu2+ ion observed in the active site coordinated by His64, His90, His99, His261, His165, His289, and His290. Structural comparisons revealed similarity between Edo-Tyr and tyrosinase from Bacillus megaterium (Bm-Tyr), with an R.M.S.D of 1.38 Å. Sequence alignment further suggested that His265 serves as the active site in Edo-Tyr, analogous to His208 in Bm-Tyr. Molecular docking analysis demonstrated that the catalytic mechanism of Edo-Tyr likely involves hydroxylation via an electrophilic attack, potentially facilitated by a tyrosine rotation event. Key residues implicated in this process include His64, His265, His90, Pro277, and Asp262. Subsequent in vitro assays using a phenol solution confirmed the recombinant Edo-Tyr's ability to degrade phenol, achieving a 48% reduction in phenol concentration with 0.3 U/µL of tyrosinase. These findings confirm the potential application of Edo-Tyr in bioremediation, specifically for phenol decontamination, highlighting its promise for industrial and environmental applications. 
Co-Authors 'Izzati, Farah Diba Abdul Rasah, Mohd Nasir Adiansyah, Dias Ahmad Furqon Apriantini, Astari Aryanti, Nova Asep Gunawan Awang, Azwan Cece Sumantri David, Viaevanilly Dedi Fardiaz Detiara, Azzahra Dian Herawati Epi Taufik Erlita Sari, Pipit Fajri, Aulia Irhamni Irma Isnafia Arief Isyana Khaerunnisa Isyana Khaerunnisa Jakaria Jakaria Karim, Norzulaiha Abd. Katrin Roosita Lindang, Herman Umbau Listyarini, Kasita Maggy Thenawijaya Mokhtar, Ruzaidi Azli Mohd Muhamad Arifin, Muhamad Muhammad Suhendra, Muhammad Murtini, Devi Nancy Dewi Yuliana Niken Ulupi Noraimah Binti Sulaiman Noraimah Binti Sulaiman Olfa Mega Ratna Sholatia Harahap Razali, Rafida Rodrigues, Kenneth F. Saiful Anwar Salundik . Seelan, Jaya Seelan Sathiya Siti Aminah Sitta Fitri Rahmadhina Soenarno, Moch. Sriduresta Tuti Suryati Yong Soo Kim Zaenal Abidin Zakiah Wulandari