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UJI PERTUMBUHAN DAN DAYA HASIL TANAMAN MUTAN (M2) CABAI RAWIT Anugrah Palupi, Delvia Dipta; Makhziah, Makhziah; Sukendah, Sukendah
Agros Journal of Agriculture Science Vol 26, No 1 (2024): Januari
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v26i1.3995

Abstract

Public demand for cayenne pepper is high. To continue to meet public demand, superior varieties are being assembled to obtain cayenne pepper plants with high yields. Breeding efforts in assembling superior varieties can be carried out using gamma ray irradiation techniques. This research was carried out with the aim of determining whether there were differences in mutant accessions resulting from irradiation compared to plants without radiation in order to determine whether there were accessions with superior characteristics in growth and yield. The research was conducted at UPN "Veteran" East Java, Surabaya. The research was structured using a Randomized Block Design consisting of 29 mutants (M2) accessions and 1 control plant (P) with 3 replications. Tests were carried out on growth parameters including plant height and number of leaves as well as plant yield parameters including number of fruit, fruit length, fruit diameter, weight per fruit, and total weight of fruit per plant. Data were analysed using analysis of variance (ANOVA) and Tukey HSD test. The results of ANOVA and Tukey HSD test showed that there were differences in the 29 mutants accessions with control plants. Accessions with superior characteristics to control plants in 6 parameters were MP1-2, MP2-5, and M2-6.Key-words: Cayenne pepper, Growth, Yield INTISARIPermintaan masyarakat akan cabai rawit tinggi. Untuk terus mencukupi permintaan masyarakat, dilakukan perakitan varietas unggul untuk mendapatkan tanaman cabai rawit dengan daya hasil tinggi. Upaya pemuliaan dalam perakitan varietas unggul dapat dilakukan dengan teknik iradiasi sinar gamma. Penelitian ini dilakukan dengan tujuan untuk mengetahui adanya perbedaan pada aksesi mutan hasil iradiasi yang dibandingkan dengan tanaman tanpa radiasi guna mengetahui adanya aksesi dengan karakter unggul dalam pertumbuhan dan daya hasil. Penelitian dilakukan di Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya. Penelitian disusun dengan Rancangan Acak Kelompok (RAK) terdiri dari 29 aksesi mutan M2 dan 1 tanaman kontrol (P) dengan 3 ulangan dan 1 sampel disetiap ulangannya. Pengujian dilakukan pada parameter pertumbuhan meliputi tinggi tanaman dan jumah daun serta parameter daya hasil tanaman meliputi jumlah buah, panjang buah, diameter buah, berat per buah, dan berat total buah per tanaman. Data dianalisis menggunakan analisis ragam (ANOVA) dan uji lanjut BNJ 5%. Hasil ANOVA dan uji lanjut BNJ 5% menunjukkan terdapat perbedaan pada 29 aksesi mutan M2 dengan tanaman kontrol. Aksesi dengan karakter lebih unggul dari tanaman kontrol pada 6 parameter adalah  MP1-2, MP2-5, dan M2-6.Kata kunci: Cabai Rawit, Daya Hasil, Pertumbuhan
Somatic Embryogenesis Induction in Coffea arabica L. by 2,4-Dichlorophenoxyacetic acid and 6-Furfurylaminopurine Tarigan, Puji Lestari; Sukendah, Sukendah; Dewanti, Felicitas Deru; Pribadi, Nathasya Yusvie; Zulmi, Shinta Nuraini
Agro Bali : Agricultural Journal Vol 7, No 3 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i3.1841

Abstract

Coffee plants typically produce for 5 to 20 years before requiring rejuvenation to maintain sustainable production levels. Tissue culture methods offer a way to efficiently produce coffee seedlings with identical genetics on a large scale, while also protecting against pests and diseases. This research focuses on developing suitable culture media for generating coffee through somatic embryogenesis, as an initial stage in creating synthetic seeds. The coffee leaf explant from young leaves. The materials were MS media, alcohol 70%, 2,4-D, Kinetin, vitamin C, Dithane M-45, Agrept, and aquades. The culture used bottles, tweezers, autoclaves, hot plates, and LAF. The treatment was the concentration of 2,4-D and Kinetin. The treatments were: 1) D0K0=0 mL.L-1 2,4-D + 0 mL.L-1 Kinetin, 2) D1K0=2 mL.L-1 2,4-D + 0 mL.L-1 Kinetin, 3) D2K0=4 mL.L-1 2,4-D + 0 mL.L-1 Kinetin, 4) D0K1=0 mL.L-1 2,4-D + 2 mL.L-1 Kinetin, 5) D0K2=0 mL.L-1 2,4-D + 4 mL.L-1 Kinetin, 6) D1K1= 2 mL.L-1 2,4-D + 2 mL.L-1 Kinetin, 7) D2K2=4 mL.L-1 2,4-D + 4 mL.L-1 Kinetin, 8) D1K2=2 mL.L-1 2,4-D + 4 mL.L-1 Kinetin, and 9) D2K1=4 mL.L-1 2,4-D + 2 mL.L-1 Kinetin. Each treatment is replicated three times, so there are 27 experimental units. The treatment D1K1 results showed that the highest percentage of life calli induction was 74%, fastest callus induction times were at 25 days, then yellowish color and a crumbly texture were the most ideal morphological variables.
PENGARUH MEDIA TANAM HAYATI PADA PENGEMBANGAN PADI LOKAL DENGAN SISTEM TANAM POLYBAG Kurniawati, Aprilia Setya; Sukendah, Sukendah; Makhziah, Makhziah
Agros Journal of Agriculture Science Vol 25, No 1 (2023): edisi JANUARI
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v25i1.2430

Abstract

Tanaman padi varietas lokal yang ditanam oleh petani diperkirakan hanya berkisar 10-15% dari jumlah plasma nutfah padi varietas lokal. Pertumbuhan masyarakat yang signifikan menyebabkan kebutuhan produksi pangan (terutama padi) mengalami peningkatan. Salah satu teknologi atau inovasi budidaya padi yang masih jarang digunakan di Indonesia adalah sistem potting/polybag. Penanaman padi lokal dengan sistem polybag membutuhkan media tanam yang tepat karena pertumbuhan padi pada polybag bergantung pada media tanamnya. Penelitian ini bertujuan untuk mengetahui pengaruh media tanam nabati pada pertumbuhan dan hasil dari pengembangan tanaman padi lokal dengan sistem polybag. Penelitian ini menggunakan padi lokal varietas Rojolele yang ditanam dengan menggunakan polybag ukuran 40 x 40 cm. Penelitian ini dilakukan di Stasiun Klimatologi Fakultas Pertanian, Universitas Pembangunan Nasional “Veteran” Gunung Anyar, Surabaya, Jawa Timur, sejak bulan November 2021 sampai Februari 2022. Penelitian ini merupakan percobaan Rancangan Acak Lengkap (RAL) non faktorial, yaitu macam media tanam, meliputi; (1) tanah + arang sekam; (2) tanah + humus bambu; dan (3) tanah + cocopeat yang semua medianya memiliki perbandingan 2:1. Setiap perlakuan diulang tiga kali. Parameter pengamatan meliputi panjang tanaman, jumlah daun, panjang akar, umur berbunga, umur panen, jumlah malai per rumpun, jumlah biji per malai, jumlah biji bernas per malai dan jumlah 100 biji. Penggunaan sistem polybag pada penggembangan budidaya padi lokal dengan macam media tanam memberikan pengaruh nyata terhadap parameter umur berbunga, umur panen dan jumlah biji per malai tanaman padi lokal.
Effect of Benzylaminopurine (BAP) and Coconut Water on the Growth of Bucephalandra sp. in Vitro Septianingsih, Alfira Damayanti; Makhziah, Makhziah; Sukendah, Sukendah
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 13 No. 4 (2024): December 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i4.1013-1022

Abstract

Tissue culture is useful as a way to obtain seeds in large quantities of seedlings in a short time as an effort to preserve Bucephalandra sp. in nature. The use of PGR has problems such as expensive prices and difficult to find, therefore its use can be replaced by natural PGR that are easy to obtain and have relatively cheap prices such as coconut water. The aim of this research was to determine the effect of Benzylaminopurine (BAP) and coconut water on the growth of Bucephalandra sp. in vitro. This research is a 2-factor factorial experiment using a completely randomized design. The factors are concentration of BAP (0; 1; 3; 5 mg/l) and concentration of coconut water (0; 50; 100 ml/l). The results showed Murashige and Skoog (MS) media with the addition of BAP 1 mg/l + coconut water 50 ml/l (4.11 shoots), BAP 5 mg/l + coconut water 50 ml/l (4.33 shoots), and BAP 3 mg/l (6.22 shoots) were able to provide the number of shoots with high results. The addition of coconut water did not affect the growth of Bucephalandra sp. MS media can grow shoots with the best results in measurements of shoot emergence time, number of roots, shoot and root length. Keywords: Aquatic plant, Endemic plant, Plant growth regulators, Tissue culture.
Effects of Differences in Installation Slope on Growth and Yield of Rice (Oryza sativa L.) Hydroponically Y.K, Cica; Sukendah, Sukendah; Makhziah, Makhziah
Agrium Vol 19 No 4 (2022)
Publisher : Faculty of Agriculture, Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/agrium.v19i4.9733

Abstract

Rice (Oryza sativa L.) is a staple food for Indonesian people to fulfill their daily needs. Rice planting is expected to be able to produce high value, but nowadays it is constrained by the increasing condition of the land. Hydroponic cultivation is the right solution because it can be done on a narrow area but the response of plants to the slope of the hydroponic land is not yet known. This study aims to determine the effect of the slope of the hydroponic installation on the growth and yield of hydroponic rice plants. The research was carried out from June to September 2022, at the experimental field of the Faculty of Agriculture, UPN "Veteran" East Java. The experiment used in this study was a Randomized Block Design with 3 replications and each replication had 10 samples. Treatment of the slope of the building consists of 0%, 3%, and 5%. Data were analyzed by the F test and continued with Duncan's multiple distance test with a level of 5%. The results showed that the installation slope of 0% gave the best results for plant and root length.