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All Journal SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Journal of Biomedicine and Translational Research Medica Hospitalia Radiant : Journal of Applied, Social, and Education Studies
Desvita Sari, Desvita
PPDS Mikrobiologi Klinik FK Univ Diponegoro/RSUP Dr. Kariadi
Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Education Health Professions Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Errors in Interpretation of Gram Stain in The First Notification from Positive BACTEC Blood Cultures in Clinical Microbiology Laboratory of Dr. Kariadi Hospital Maryani, Maryani; Sari, Desvita; Wahyutomo, Ridha; Masfiyah, Masfiyah
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 4, No 1 (2012): Januari-Juni 2012
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.067 KB) | DOI: 10.30659/sainsmed.v4i1.381

Abstract

Background: Blood cultures in conjunction with the initial Gram stain of positive cultures have often been considered the “gold standard” for the diagnosis of bacteremia. When blood cultures turn positive, the attending physicians are usually notified immediately about Gram stain findings. However, information on the accuracy of Gram staining is very limited. We examined the error of preliminary blood culture reports provided by a local laboratory in an observational study.Design and Method: This was an observational study with a cross sectional approach. In this study, 369 blood cultures were examined. The positive blood cultures (135 samples) were then examined by Gram stain. Blood cultures handled on Bactec 9050, while the Gram stain was done in standard procedure Gram. Interpretation errors of Gram stain were confirmed by cultures result.Result: During one month (April 2011) we examined 369 blood cultures which 135 are positive (36.5%). Positive blood cultures were misread for 6 (4.4%) of 135 patients, they were two read as gram positive cocci had gram negative organisms by culture which were Acinetobacter baumannii, one read as gram positive bacilli had gram negative bacilli by culture which was Klebsiella pneumoniae. One isolate read as gram negative bacilli had gram positive bacilli which was Bacillus species, while two sample read as gram negative bacilli only had polymicrobial by culture, of these one isolate grew to be Enterobacter aerogenes and Staphylococcus aureus and the other were Escherichia coli and Acinetobacter spp.Conclusion: The overall 4.4% error rate of misinterpreted Gram stains from positive blood culture bottles is relatively high, so laboratory professionals and clinical microbiologist must be aware of the potential types of error that occur (Sains Medika, 4(1):23-29).
Comparison of SARS-CoV-2 Variant Screening and Whole Genome Sequencing at an Indonesian Tertiary Hospitals Hapsari, Rebriarina; Kesumayadi, Irfan; Sari, Desvita; Anjarwati, Dwi Utami; Alfiyuliani, Nesia Hani; Mujahidah, Mujahidah; Sari, Iva Puspita; Hadi, Purnomo
Journal of Biomedicine and Translational Research Vol 9, No 3 (2023): December 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i3.19147

Abstract

Background: The global COVID-19 pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), experienced a surge in cases with the emergence of the Omicron variant. Despite increasing vaccination coverage, Indonesia witnessed peaks in COVID-19 cases. Variant screening and whole genome sequencing (WGS) play a crucial role in identifying SARS-CoV-2 variants and monitoring their spread.Objective: The objective of this study was to compare variant screening results with WGS data, assess the prevalence of subvariants, and analyze their correlation with demographic and cycle threshold (CT) values.Methods: Between November 7th and 18th, 2022, variant screening and WGS were conducted on samples with CT values below 30. Variant screening utilized the mBioCov-19+ VarScreen assay, while WGS was performed on the Oxford Nanopore Technologies (ONT) platform. Bioinformatics analysis was performed using epi2melabs. Demographic data and CT values were analyzed. Results: Out of 89 subjects, all tested positive for the Omicron variant through variant screening. The variant screening identified two subvariants: Omicron BA.2 (64%) and Omicron B.1.1.529.1 (36%). WGS revealed that the XBB subvariant was the most dominant (52.8%), followed by BQ.1 (22.5%) and BA.5 (13.5%). When VarScreen indicated BA.2, the majority of WGS results showed XBB (82.5%), while for B.1.1.529.1, the majority of WGS results were BQ.1 (59.4%), followed by BA.5 (37.5%). XBB was the most prevalent variant in both females and males, while BQ.1 was more dominant in females (80%). No infections were detected among children aged 1-5 years. All variants had CT values below 24.Conclusion: Variant screening provides accurate and quick results for detecting the Omicron variant in laboratories without WGS capacity. However, it is important to continuously update the screening methodology based on the prevailing circulating variants. During the study period, XBB emerged as the predominant subvariant of the Omicron variant.
Characteristics and Risk Factor of Multidrug-Resistant-Organism Infection in the Pediatric Intensive Care Unit of Dr. Kariadi Hospital Semarang Arkhaesi, Nahwa; Supriatna, Moh; Istanti, Yusrina; Sari, Desvita; Azzahra, Safira Rizqi
Medica Hospitalia : Journal of Clinical Medicine Vol. 11 No. 1 (2024): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36408/mhjcm.v11i1.989

Abstract

BACKGROUND: The Pediatric Intensive Care Unit (PICU) that treats critical patients with various invasive procedures is a potential place for Multidrug Resistant Organism (MDRO) infections. This spesific unit, especially in tertiary care facilities may have higher prevalence of MDRO than non-PICU settings, causes serious disease and mortality. AIMS: To describe the characteristics and risk factor of MDRO infection in the PICU of dr. Kariadi Hospital Semarang, METHOD: Retrospective study in patients aged 1 month - 18 years with growth of germs on culture results during 2021-2022. Demographic data, use of central venous catheters and ventilator, length of stay, duration of antibiotic usage, and history of major surgery were investigated from medical records. The amount of risk expressed by the Odds Ratio (OR).RESULT: From 544 culture examinations, 199 (36.5%) germs grew, consisting of 79 MDRO isolates (39.7%) and 120 non-MDRO isolates (60.3%). Gram-negative bacteria were found in 140 samples (70.4%) with Klebsiella pneumoniae as the most common germ (15.1%) and respiratory tract infection (56.3%) being the most common infection diagnosis. As many as 24 of 30 (80%) isolates that grew Klebsiella pneumoniae were MDRO. Mechanical ventilator use (OR 2.15; 95% CI 1.07 – 4.3; p=0.043) and length of stay in the PICU (OR 2.44; 95% CI 1.21 – 4.96; p=0.018) are risk factors for MDRO infection.CONCLUSION: Klebsiella pneumoniae is the most common germ found from culture isolates and respiratory tract infection is the most common diagnosis. The use of mechanical ventilators and length of stay in the PICU are risk factors for MDRO infection. 
The Effectiveness of Blended Learning in English Speaking Skill for Undergraduate in 4.0 Sari, Desvita; Sofyan, Rudy; Nasution, Ely Hayati
RADIANT: Journal of Applied, Social, and Education Studies Vol. 2 No. 2 (2021): RADIANT Journal of Applied, Social, and Education Studies
Publisher : Politeknik Harapan Bangsa Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52187/rdt.v2i2.51

Abstract

This research deals with the students’ achievement of speaking skills and perception towards Implementing Individual Rotation of blended learning. The writers discuss the effectiveness of blended learning implemented in the pre-test and post-test of English speaking skill achievement of the 2nd semester of English Department students of the University of Sumatera Utara and their perception of it. This research aims to find out how the Individual Rotation Model of blended learning improves students’ English speaking skill achievement using the Learn Social platform and to find out students’ perceptions after implementing blended learning. In conducting this research, the writers apply descriptive with a mixed-method (Creswell & Plano Clark, 2011) analyzing the students’ achievement found in the pre-test and post-test and giving the online questionnaire to assess their perception towards blended learning. The data shows that the student's achievement in the post-test (Mean=87%) was higher than the pre-test (Mean=85%) out of 50 students. According to the data, 90% of the 42 students gave a positive response to blended learning. The result showed Individual Rotation Model improves the English speaking skill achievement especially in the components of speaking skill namely, vocabulary, grammar, and comprehension, and effective to be implemented for undergraduate students in the era of industrial revolution 4.0.
Co-Authors Alfiyuliani, Nesia Hani Azzahra, Safira Rizqi Dwi Utami Anjarwati Kesumayadi, Irfan Maryani Maryani Masfiyah Masfiyah, Masfiyah Mujahidah Mujahidah Nahwa Arkhaesi Nasution, Ely Hayati Purnomo Hadi Rebriarina Hapsari Ridha Wahyutomo Sari, Iva Puspita Sofyan, Rudy Supriatna, Moh Yusrina Istanti