Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

2.338

P-Index

This Author published in this journals

All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Medali Jurnal : Media Dental Intelektual MANDALA of Health Journal of Biomedicine and Translational Research INDONESIAN JOURNAL OF PHARMACY Jurnal Kedokteran Gigi Universitas Padjadjaran Journal Of Vocational Health Studies Jurnal Pengabdian Pada Masyarakat Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Jurnal Health Sains JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Mandala of Health : A Scientific Journal Medical and Health Journal Molekul: Jurnal Ilmiah Kimia

Dwi Utami Anjarwati

Department Of Microbiology, Faculty Of Medicine Universitas Jenderal Soedirman, Purwokerto, Central Java, Indonesia

Author-ID : 7846253

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Education Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

Published : 17 Documents Claim Missing Document

Claim Missing Document

Articles

1 2

IDENTIFIKASI VANCOMYCIN RESISTANT Staphylococcus aureus (VRSA) PADA MEMBRAN STETOSKOP DI RUMAH SAKIT MARGONO SOEKARJO PURWOKERTO Darmawan, Anton Budi; Anjarwati, Dwi Utami
MANDALA of Health Vol 4, No 2 (2010): Mandala Of Health
Publisher : Jurusan Kedokteran FK Unsoed

Show Abstract | Download Original | Original Source | Check in Google Scholar

Vancomycin-resistant Staphylococcus aureus (VRSA) was first discovered in 2002 by a clinician inthe USA. Vancomycin resistance mediated by the van gene which will result in a change in the terminalS.aureus cell wall. Data on epidemiology of VRSA from RSUD Margono Soekarjo (RSMS), Purwokertogenerally were not available. The purpose of the study was identify VRSA on stethoscopes membrane inRSUD Margono Soekarjo (RSMS), Purwokerto. A Laboratory observational study with cross sectionaldesign was conducted to 69 stethoscopes membrane in RSUD Margono Soekarjo (RSMS), Purwokerto.Isolates were taken from stethoscopes membrane for Vancomycin discs testing to detect VRSA. The resultshowed that 10 of 64 isolates with S.aureus positive (15,6%) found on stethoscopes membrane were VRSA.In conclusion, VRSA was found on stethoscopes membrane in RSUD Margono Soekarjo (RSMS),Purwokerto.

IN VITRO EFFECT OF Chloroprocta SP. MAGGOTS SECRETION ON Staphylococcus epidermidis BIOFILM AND THE EXPRESSION LEVEL icaA OF GENE Anjarwati, Dwi Utami; Setiawati, Setiawati; Mujahidah, Mujahidah; Hapsari, Rebriarina; Nuryastuti, Titik
Indonesian Journal of Pharmacy Vol 25 No 2, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Biofilm formation and the expression of icaA gene can be induced by environment conditions that are potentially toxic for bacterial cells. The effect of green flies maggots secretion to biofilm was studied some years ago to investigate in vitro effect of secretion of Chloroprocta sp. maggots on the formation of Staphylococcus epidermidis biofilm (phenotype) and the expression level of icaA gene (genotype) for indicating its mechanism on bacterial biofilm eradication. Microtiter plate biofilm assay was used to measure the effect of Chloroprocta sp. maggots secretion at various concentration on S. epidermidis biofilm. The expression level of icaA gene was performed by Real TimePCR using lightcycler method. The biofilm susceptibility test was done against maggots excretion/secretion using MTT assay. Whereas planktonic susceptibility testing was carried out using Kirby Bauer method. In the presence of maggots secretion at low concentration (5%), biofilm formation of S. epidermidis 734 was induced. In contrast, the expression level of icaA gene in production of maggots excretion/ secretion at concentration of 5% was lower than that of without maggots secretion (1/2 Fold). Eradication of bacterial biofilm was demonstrated after 48h incubation (MD=-0,011;P<0,05), but planktonic cell. In vitro difference effect of the Chloroprocta sp. maggots secretion at low concentration to phenotype and genotype of S. epidermidis biofilm showed that the possibility of maggots secretion ability to eradicate bacterial biofilm was not mainly due to the expression level of icaA gene.

Profile of Biofilm-Producing Staphylococcus epidermidis from Intravenous Catheter Colonisation at Prof. Dr. Margono Soekarjo Hospital Purwokerto Dwi Utami Anjarwati; Wahyu Siswandari; IDSAP Peramiarti
Indonesian Journal of Pharmacy Vol 30 No 1, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Biofilm- producing Staphylococcus epidermidis has evolved to be a significant human pathogen, particularly in the use of medical devices such as an intravenous catheter. Furthermore, biofilm-producing bacteria 10-1000 fold less susceptible to several antimicrobial agents than free-bacteria. This simple survey aimed to describe the profile of biofilm-producing S. epidermidis from intravenous catheter colonization of some patients in surgical and internal medicine wards at the hospital Margono Soekarjo, Purwokerto, and the antibiotics resistance pattern. A vitek® 2 compact (Enseval Medika Prima) was performed to identify the bacterial species and to examine the 73 antibiotics for understanding the resistance pattern automatically. Microtiter plate biofilm assay with crystal violet staining was performed to measure biofilm optical density (OD) for analyzing the biofilm production capabilities. A scanning electron microscopy (SEM) was done to compare the thickness of ultrastructure of biofilm-producing S. epidermidis visually. The present study found that 2 of 8 Gram-positive bacteria (25%) were biofilm-producing S. epidermidis. One of S. epidermidis was moderate whereas the other was high biofilm-producing bacteria. Images of SEM showed that a high biofilm-producing S. epidermidis has a thicker ultrastructure of biofilm than the moderate biofilm-producing, whereas a control, the weak biofilm-producing S. epidermidis ATCC 12228 has the least biofilm. Both of S. epidermidis strains were sensitive to Gentamicin, Moxifloxacin, Quinupristin/Dalfopristin, Linezolid, Vancomycin, Doxycycline, Minocycline, Tetracycline, Tigecycline, and Nitrofurantoin. Furthermore, both S. epidermidis strains were resistant to the other (63) antibiotics. In conclusion, two strains of S. epidermidis in this study have different capabilities to form the biofilm which were showed that high biofilm-producing strain was thicker than moderate biofilm-producing strain by scanning electron microscopy. However, both of them were resistant to the same number of antibiotics.

Efek antibakteri ekstrak larva Chrysomya megacephala terhadap Enterococcus faecalis sebagai alternatif bahan irigasi saluran akarAntibacterial effect of Chrysomya megacephala larva extract on Enterococcus faecalis as a root canal irrigant alternative Rizka Hidayati; Ari Asnani; Muhamad Salman Fareza; Dwi Utami Anjarwati
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 32, No 2 (2020): Agustus 2020
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v32i2.27094

Pendahuluan: Desinfeksi saluran akar dilakukan dengan mengaplikasikan bahan irigasi saluran akar pada perawatan endodontik. Selama ini, natrium hipoklorit (NaOCl) menjadi protokol bahan irigasi saluran akar karena bersifat antimikroba. Penggunaan NaOCl memiliki kekurangan karena kurang adekuat dalam mengeliminasi bakteri persisten dalam saluran akar gigi, seperti Enterococcus faecalis. Upaya pengeliminasian E. faecalis yang dapat dilakukan yakni dengan mengkombinasikan NaOCl dengan bahan irigasi lain atau menggunakan bahan alternatif lain. Salah satu bahan alam yang memiliki efek antibakteri adalah ektrak larva dari lalat hijau (Chrysomya megacephala). Produk eksretori dan sekretori larva instar ke-3 C. megacephala mengandung protease serin yang memiliki efek antibakteri. Tujuan penelitian untuk menganalisis efek antibakteri ekstrak larva C. megacephala dalam menghambat pertumbuhan E. faecalis. Metode: Jenis penelitian eksperimental laboratorium. Ekstrak larva C. megacephala dibuat dengan konsentrasi 0-100%. Efek antibakteri diketahui dengan pengukuran nilai Minimal Inhibitory Concentration (MIC) dan Minimal Bactericidal Concentration (MBC). Nilai MIC diukur dengan metode pengenceran microbroth dalam cawan microtiter 96-well. Nilai MBC diukur dari hasil pengukuran MIC yang di-plating pada media kultur Mueller Hinton Agar (MHA) dengan metode total counting. Hasil: Nilai MIC diperoleh pada ekstrak larva 50% (0,5x106mg/L) (p>0,05) sedangkan nilai MBC tidak ditemukan. Simpulan: Ekstrak larva C. Megacephala tidak memiliki efek yang dapat menghambat pertumbuhan Enterococcus faecalis.Kata kunci: Antibakteri, natrium hipoklorit, Chrysomya megacephala, Enterococcus faecalis. ABSTRACTIntroduction: In endodontic treatment, the root canal is disinfected by applying root canal irrigant. Commonly, sodium hypochlorite (NaOCl) is used as the protocol of the root canal irrigant due to its antimicrobial effects. The usage of NaOCl has limitation because it is inadequate in eliminating persistent bacteria in the root canal, such as Enterococcus faecalis. The effort to eliminate E. faecalis is by combining NaOCl with other irrigant or using an alternative material. One of the natural products with antibacterial effects is the larva extract of greenfly (Chrysomya megacephala). The excretory and secretory products of the third instar of the larva contain serine protease that has been known for the antibacterial effects. The purpose of this study was to analyse the antibacterial effect of Chrysomya megacephala larva extract in inhibiting the E. faecalis bacteria. Methods: Experimental laboratory research was conducted towards an extract of C. megacephala larva, which was prepared with a concentration of 0-100%. The antibacterial effect was determined by measuring the value of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The MIC values were measured by the micro broth dilution method in a 96-well microtiter dish. The MBC value was measured from the results of the MIC measurement plated on the Mueller Hinton Agar (MHA) culture media using the total counting method. Results: The MIC values obtained in 50% larva extract was 0.5x106mg / L (p > 0.05) while the MBC value was not found. Conclusion: Chrysomya megacephala larva extract does not have the effect in inhibiting the Enterococcus faecalis.Keywords: Antibacterial, sodium hypochlorite, Chrysomya megacephala, Enterococcus faecalis.

Detection of icaAD Gene of Biofilm-Producing Staphylococcus aureus Carriage Isolates Obtained from Health Care Workers and Healthy Communities in Banyumas, Indonesia Gembong Satria Mahardhika; Metta Ayu Susanti; Lantip Rujito; Dwi Utami Anjarwati
Journal of Biomedicine and Translational Research Vol 6, No 1 (2020): April 2020
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v6i1.6135

Background: Asymptomatic biofilm-producing Staphylococcus aureus carriage play a pivotal role as a reservoir pathogen and increase the transmission rate in hospital as well as in healthy community. Biofilm- producing S. aureus which is regulated by the the ica AD gene reduce the antimicrobial ability in eliminating the pathogen. Objective: The aim of this study was to detect the icaAD gene of biofilm-producing Staphylococcus aureus carriage isolates obtained from healthcare workers and healthy Community in Banyumas, Indonesia.Methods: This descriptive observational study enrolled 60 healthcare workers and 60 healthy communities in Banyumas district. Antibiotic susceptibility test was using disc diffusion according to Clinical laboratory Standard Institute (CLSI) 2019. Biofilm-producing ability identified by using microtiter plate biofilm assay and the positivity of icaAD gene was performed by using PCR method.Results: The results showed that one of 60 healthcare workers (0,017%) showed MRSA, four of 60 healthcare workers (0,07%) were MSSA and 2 samples from community (0,03%) were MSSA. Total of 7 samples underwent biofilm examination, one sample was moderate biofilm, two samples were weak biofilm, and four samples were no biofilm. It was known that three biofilm-producing S.aureus were positive ica A/D gene.Conclusion: The ica A/D gene was found positive in both biofilm-producing MRSA and MSSA strain from both healthcare workers group and the healthy communities group. The presence of icaAD genes in both strains shows the potential for antibiotic resistance in these strains regulated by different mechanisms.

Peningkatan Penggunaan Antibiotik Secara Bijak Melalui Intervensi Alih Pengetahuan Untuk Merubah Persepsi Masyarakat di Kabupaten Banyumas Dwi Utami Anjarwati; Fajar Wahyu Pribadi; Idsap Peramiarti
Jurnal Pengabdian Pada Masyarakat Vol 2 No 2 (2017)
Publisher : Universitas Mathla'ul Anwar Banten

IMPROVED USE OF ANTIBIOTIC BY USING TRANSFER OF KNOWLEDGE INTERVENTIONS TO CHANGE THE COMMUNITY PERCEPTION IN BANYUMAS DISTRICT. The use of antibiotics without indication by the medical community and the community became the trigger factor of increasing the incidence of antibiotic resistance in Indonesia. Meanwhile by 20150 antibiotic resistance is predictedto be the leading cause of death in the world. Community devotion was aimed at improving the understanding of society so that that is a change of perception about the use of antibiotic wisely. The target respondents of devotion consists of member of the family welfare empowerment group (PKK). A seriaes of activities undertaken were pre and post test to measure respondents' understanding of antibiotic use before and after intervention in the transfer of knowledge. The questionnaire used previously tested its validity by Pearson product moment test and its reliability by Alpha-Cronbach. The Sharpening of material obtained in knowledge transfer was done with role play and discussion. The result of T test analysis to score of quesionnaire before and after knowledge transfer showed significant p value (p = 0,049). The conclusion was that the transfer of knowledge can change the perception of PKK members about the wise use of antibiotics.

Peningkatan Penggunaan Antibiotik Bijak pada Kelompok Pemberdayaan Wanita di Daerah Perkotaan di Kabupaten Banyumas Melalui Tindakan Intervensi KAP (Knowledge, Attitude, Practice) Dwi Utami Anjarwati; Rahmawati Wulan Sari; Diyah Woro Dwi Lestari
Jurnal Pengabdian Pada Masyarakat Vol 4 No 2 (2019)
Publisher : Universitas Mathla'ul Anwar Banten

INCREASING OF THE USE OF WISE ANTIBIOTIC IN WOMAN'S EMPOWERMENT GROUP IN THE URBAN AREA OF BANYUMAS REGENCY BY PROVIDING INTERVENTION OF KAP (KNOWLEDGE, ATTITUDE, PRACTICE). Unwise use of antibiotic behavior in the community contributes significantly to the increase in the incidence of antibiotic resistance. Antibiotic resistance has become a major health problem throughout the world that must receive attention both in hospitals and in the community. The purpose of this community service is as an effort to increase the use of wise antibiotics in women's empowerment groups in urban areas in Banyumas Regency by intervening Knowledge, Attitude, Practice (KAP). The target audience was an urban community group in Banyumas regency, namely all members of the Rambutan women's empowerment group located at Jl. Kalijaga, Berkoh, Purwokerto. The activities carried out for KAP intervention were knowledge transfer, and educational video included antibiotics, the wise use of antibiotics, and the dangers of antibiotic resistance. The results of the KAP intervention were measured by questionnaires containing statements from aspects of Knowledge, attitude, practice before and after KAP intervention activities. Each statement in the questionnaire was assessed with a Likert scale and tested for its validity and reliability. Data were analyzed descriptively while testing the results of questionnaires I and II were carried out by paired T-Tests. Data that can be analyzed are 39 respondents. The results of the questionnaire analysis showed an increase in KAP about antibiotic use before and after KAP interventions in women's empowerment groups in urban areas of Banyumas Regency. The article concluded that KAP intervention actions could achieve an increase in the use of wise antibiotics in women's empowerment groups in urban areas in Banyumas Regency.

Antibiotic resistance of biofilm-producing bacteria from sepsis patients in Prof. Dr. Margono Soekarjo Hospital, Purwokerto, Central Java Rani Afifah Nur Hestiyani; Wahyu Siswandari; Vitasari Indriani; I Dewa Sang Ayu Putu Peramiarti; Dwi Utami Anjarwati; Hermin Prihartini
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 54, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19106/JMedSci005402202207

Sepsis is a life-threatening organ dysfunction induced by the body's response to infection and is a significant cause of critical illness and death in hospitals. Bacteria are the most common pathogens that cause sepsis, and their ability to form biofilms increases their resistance to antibiotics. As a result of the failure of antibiotic administration therapy, the severity and pain of sepsis worsen. The study used a descriptive research design to determine the antibiotic resistance pattern of biofilm-producing bacteria from clinical isolates of sepsis patients. Using the BacT/Blood Culture System Alert, all patients suspected of sepsis in the intensive care unit of Prof. Dr. Margono Soekarjo General Hospital Purwokerto were examined for blood cultures between March and July 2018. These were then identified and tested for antibiotic resistance with the Vitek 2 Compact. Biofilm formation was detected utilizing the microtiter plate assay method, and the data were analyzed using a frequency distribution table. The results obtained 12 bacterial isolates, with Escherichia coli (41.67%), Staphylococcus haemolyticus (33.33%), Klebsiella pneumoniae ssp pneumoniae, Enterobacter cloacae complex, and Acinetobacter baumannii complex (8.33%) as the most common bacteria. All gram-negative bacteria (more than 80%) were resistant to ampicillin, cefoxitin, ceftazidime, ceftriaxone, aztreonam, and trimethoprim but were sensitive to meropenem (100%). Gram-positive bacteria were resistant to cefoxitin, benzylpenicillin, oxacillin, ciprofloxacin, erythromycin, and clindamycin (100% each). However, they were sensitive to tigecycline, nitrofuran, quinupristin, linezolid, vancomycin, and tetracycline (100% each). Gram-negative bacteria formed 50% biofilms, and 50% did not, whereas gram-positive bacteria produced 100% biofilms. In conclusion, bacteria clinical isolates of septic patients from Prof. Dr. Margono Soekarjo General Hospital Purwokerto are multiresistant to more than six types of antibiotics and produce weak to moderate biofilms, which can promote antibiotic resistance.

The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in Banyumas Metta Ayu Susanti; Gembong Satria Mahardhika; Lantip Rujito; Anton Budhi Darmawan; Dwi Utami Anjarwati
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 3, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss3.art7

Background: Methicillin-Resistant Staphylococcus aureus (MRSA) has become a major pathogene and its incidence continues to increase in various countries. The resistance to methicillin is caused by the mecA gene that encodes the expression of Penicillin Binding Protein 2a (PBP2a) so it has a low affinity for beta-lactam antibiotics. One of the factors that can increase the incidence of MRSA is inappropriate antibiotics use. Objective: This study is to identify presence of the mecA gene in MRSA and to compare histories of inappropriate antibiotic uses of healthcare workers and communities in Banyumas.Methods: This was a cross-sectional study conducting 120 samples from nasal swabs. Isolates of MRSA tested by bacteriological examinations and PCR of the mecA genes. Histories of antibiotic uses were assessed by using questionnaires and then analysed descriptively by using Fisher Exact test (SPPS, version 20).Result: One sample of S. aureus from the microbiology examination (0.83%) was an MRSA although there was no a mecA gene identified by using the PCR method. 66.1% of the healthcare workers and 88,3% of the communities used antibiotics inappropriately. The results showed that there was a statistically significant difference in the histories of irrational uses of antibiotics (p?0.05).Conclusion: A number of the communities who used inappropriate antibiotics was higher than the health workers. Furthermore, one MRSA strain with a negative mecA gene was found in the health worker group.

Relationship Between Esbls Production And Virulence Factors Of Fima And Papc Gene In Uropathogenic Escherichia Coli Isolation From Private Hospital Ade Hasan Basri; Anang Kurniawan; Joko Setyono; Dwi Utami Anjarwati
Jurnal Health Sains Vol. 3 No. 12 (2022): Jurnal Health Sains
Publisher : Syntax Corporation Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/jhs.v3i12.695

Escherichia coli is one of the opportunistic pathogenic that occupies the highest position causing the incidence of UTI. Fimbriae, particularly type 1 and P fimbriae, are the most commonly implicated bacterial cell surface virulence factors. The production of ESBL and virulence factors in E.coli bacteria causes chronicity, persistence, and recurrence of infections that cause high morbidity and mortality. Therefore, this study was conducted to explain the relationship between ESBL production and its virulence factors in E.coli bacteria. The design of this research is analytically observational with a cross-sectional approach conducted from March to May 2021. A total of 40 E. coli strains were isolated and collected from urine samples of UTI patients who were admitted to the hospital. in a private Hospital in Banyumas Region in Central Java, Indonesia. The HiChrome ESBL Agar Base media was used to screen for ESBL-Producing E. coli. Identification of firmA and papC genes was performed by using the PCR method. All urine samples diagnosed with UTI were examined for ESBL production. As many as 25% of E.coli were ESBL-production. All isolates showing positive E.coli ESBL results were then analyzed for fimA and papC genes using the PCR method. The results obtained 100% fimA gene and 80% papC gene. The conclusion is that there is a strong relationship between ESBL production with fimA and papC genes.

Co-Authors Abdi Rahman Nursyamsi Ade Hasan Basri Agus Fitrianto, Agus Agustina, Nenden Nursyamsi Alfiyuliani, Nesia Hani Anang Kurniawan Annisa Permata Dinda Anton Budhi Darmawan Anton Budi Darmawan, Anton Budi Ari Asnani Bagus, Zahran Uzla Attaqi Desvita Sari, Desvita Diyah Woro Dwi Lestari Eman Sutrisna Fajar Wahyu Pribadi Fanni Kusuma Djati Fareza, Muhamad Salman Gembong Satria Mahardhika Gembong Satria Mahardhika Hermin Prihartini I Dewa Sang Ayu Putu Peramiarti IDSAP Peramiarti Idsap Peramiarti Joko Setyono Joko Setyono Kesumayadi, Irfan Kurniawan, Anang Lantip Rujito Maulena, Ufik Metta Ayu Susanti Metta Ayu Susanti Meylida Ichsyani Mujahidah Mujahidah Mujahidah, Mujahidah Naufalin, Rafa Purnomo Hadi Qodri Santosa, Qodri Rahmawati Wulan Sari Rahmawati Wulansari Rani Afifah Nur Histiyani Rebriarina Hapsari Rizka Hidayati Sari, Iva Puspita Setiawati, Setiawati Thianti Sylviningrum Titik Nuryastuti Vitasari Indriani Wahyu Siswandari

Title

Found 17 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 17 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 17 Documents
Search