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Phytochemical Profile and Antioxidant Activity of Bandotan (Ageratum conyzoides L.) Leaves from East Kalimantan Hajar, Ayu Iradha Umi; Indah, Marwa; Miranda, Esti; Nuraini, Indah; Kusuma, Aldi; Erika, Farah
Hydrogen: Jurnal Kependidikan Kimia Vol. 13 No. 3 (2025): June 2025
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v13i3.16179

Abstract

Ageratum conyzoides L. is a wild plant known to contain secondary metabolites with potential antioxidant activity, such as flavonoids and phenolics. However, the content and activity of active compounds in the leaves of A. conyzoides growing in the Berau region, East Kalimantan, have not been extensively studied. This study aims to evaluate the phytochemical content and antioxidant activity of ethanol extracts from A. conyzoides leaves collected from the Batu Putih area, Berau. The samples, in the form of fresh leaves, were dried and ground before being extracted using the maceration method with 96% ethanol as the solvent. The resulting extract was then subjected to phytochemical screening to identify groups of active compounds and tested for antioxidant activity using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, with absorbance measured at a wavelength of 420 nm. Phytochemical screening results indicated the presence of alkaloids, phenolics, and tannins, while flavonoids, terpenoids, and triterpenoids were not detected. The antioxidant assay showed that the extract exhibited strong activity, with an IC50 value of 61.89 ppm, although it was still higher than that of vitamin C, which had an IC50 value of 26.77 ppm. In conclusion, the ethanol extract of A. conyzoides leaves from Batu Putih has potential as a natural antioxidant source and is worthy of further development in the pharmaceutical field.
Acid Phosphatase and Zinc Tests Are Effective for Semen Examination and Identification to Prove Intercourse Yudianto, Ahmad; Wibowo, Ariyanto; Nuraini, Indah; Aung, Htet Htet
Folia Medica Indonesiana Vol. 56, No. 3
Publisher : Folia Medica Indonesiana

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Abstract

The presence of spermatozoa in vagina is a definite sign of sexual intercourse. However, sometimes microscopic examination does not find spermatozoa or reveals a false negative result. This is influenced by many factors, including the absence of ejaculate in the vaginal canal. In addition, there are other factors such as oligo/azoospermia, vasectomy, degeneration of sperm due to time, incorrect sampling, and improper storage. Therefore, examination of the other components of the ejaculate, ie. the enzyme acid phosphatase, choline and spermin, is important. Compared with spermatozoa, the enzyme phosphatase, choline and spermin have lower evidentiary value because these three components are less specific. However, the level of phosphatase enzyme found in the vagina is much lower than phosphatase enzyme that comes from prostate gland. In this study, as many as 192 samples in the form of patches with sperm/semen stains were tested with acid phosphatase test and zinc test through direct and indirect examination. In the first method, washing was carried out on day 1, day 7, and month 3, and testing was carried out after each washing. In the second method, washing was carried out simultaneously and testing was carried out on day 1, day 7 and month 3. Washing was carried out using 7 types of detergent for each 4 patch samples, and washing using water as control. The results showed very low sensitivity (0.186) and very high specificity (100%). This showed that both tests had high specificity values. Acid phosphatase test specifically showed the presence of the enzyme phosphatase, while zinc test specifically showed the presence of zinc in semen. This phosphohydrolase-phosphatase enzyme is easily degraded due to external factors, including temperature, humidity, and chemicals, ie. the element SDS (Sodium Dodecyl Sulfate) that has the ability to cut enzymes. The weakness of the acid phosphatase test is that this enzyme is easily degraded, either partially or completely, due to external factors, such as temperature, humidity, heat, and the presence of chemicals.
Bedaya Duradasih, Sebuah Ekspresi Budaya pada Masa Sunan Pakubuwana III-IV di Keraton Surakarta Nuraini, Indah; Supriyanti, Supriyanti
Dance and Theatre Review Vol 4, No 2: November 2021
Publisher : Institut Seni Indonesia Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (730.228 KB) | DOI: 10.24821/dtr.v4i2.6452

Abstract

Bedaya Duradasih: A Cultural Expression During the time of Sunan Pakubuwana III-IV in the Surakarta Palace. Bedaya Duradasih is a form of bedaya dance created at the Surakarta Palace. Its creator was Sunan Pakubuwana IV who at that time, still had the title of Kanjeng Gusti Pangeran Adipati Anom. Sunan Pakubuwana IV was a king who had a very significant artistic spirit, so it cannot be denied that artistic and cultural life was very flourishing during his reign. Bedaya Duradasih was one of the cultural expressions at that time. The created expression was inspired by the mood of being infatuated/falling in love with the Madurese princess. Looking at the words Duradasih and Madura can be connected with several opinions regarding the meaning of the word Duradasih. It is said that Duradasih derived from the words Dura and dasih, which mean a woman from Madura. Then it is also mentioned from the words Dara and dasih, which means girl/virgin who brings happiness. Furthermore, it is interpreted from the words Dora and dasih, which means lying for political interests at that time, which ended up with a love affair. From these descriptions, it can be concluded that Bedaya Duradasih reflects one of the cultural expressions.Keywords: bedaya; Surakarta; expression