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Acid Phosphatase and Zinc Tests Are Effective for Semen Examination and Identification to Prove Intercourse Yudianto, Ahmad; Wibowo, Ariyanto; Nuraini, Indah; Aung, Htet Htet
Folia Medica Indonesiana Vol. 56, No. 3
Publisher : Folia Medica Indonesiana

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Abstract

The presence of spermatozoa in vagina is a definite sign of sexual intercourse. However, sometimes microscopic examination does not find spermatozoa or reveals a false negative result. This is influenced by many factors, including the absence of ejaculate in the vaginal canal. In addition, there are other factors such as oligo/azoospermia, vasectomy, degeneration of sperm due to time, incorrect sampling, and improper storage. Therefore, examination of the other components of the ejaculate, ie. the enzyme acid phosphatase, choline and spermin, is important. Compared with spermatozoa, the enzyme phosphatase, choline and spermin have lower evidentiary value because these three components are less specific. However, the level of phosphatase enzyme found in the vagina is much lower than phosphatase enzyme that comes from prostate gland. In this study, as many as 192 samples in the form of patches with sperm/semen stains were tested with acid phosphatase test and zinc test through direct and indirect examination. In the first method, washing was carried out on day 1, day 7, and month 3, and testing was carried out after each washing. In the second method, washing was carried out simultaneously and testing was carried out on day 1, day 7 and month 3. Washing was carried out using 7 types of detergent for each 4 patch samples, and washing using water as control. The results showed very low sensitivity (0.186) and very high specificity (100%). This showed that both tests had high specificity values. Acid phosphatase test specifically showed the presence of the enzyme phosphatase, while zinc test specifically showed the presence of zinc in semen. This phosphohydrolase-phosphatase enzyme is easily degraded due to external factors, including temperature, humidity, and chemicals, ie. the element SDS (Sodium Dodecyl Sulfate) that has the ability to cut enzymes. The weakness of the acid phosphatase test is that this enzyme is easily degraded, either partially or completely, due to external factors, such as temperature, humidity, heat, and the presence of chemicals.
Analysis of the Need for Interactive Learning Media Based on Canva Integrated with Wordwall to Increase Student Interest in Learning about Electrolyte and Non-electrolyte Solutions Nuraini, Indah; Muflihah, Muflihah; Hartati, Yuli
Paedagoria : Jurnal Kajian, Penelitian dan Pengembangan Kependidikan Vol 17, No 2 (2026): April
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/paedagoria.v17i2.38362

Abstract

Students’ low interest in learning about electrolyte and non-electrolyte solutions is caused by the abstract nature of the concepts and the limited use of interactive learning media. This study aims to analyze students’ needs regarding the development of interactive learning media based on Canva integrated with Wordwall to enhance learning interest. The study employed a quantitative descriptive design with a sample of 42 twelfth-grade students and one chemistry teacher at SMA Negeri 9 Samarinda, selected through purposive sampling. Data were collected via questionnaires and interviews, then analyzed using descriptive percentage statistics. The results showed that all 21 questionnaire items were valid with very high reliability (Cronbach’s Alpha = 0.939). A total of 84.52% of students needed media capable of visualizing concepts, 80.95% possessed technological readiness, and 76.17% were ready to use interactive media. The findings also indicate a correlation between learning interest, the use of learning media, and the need for interactive media, where media limitations impact suboptimal student engagement. Interview results reinforce the need for media featuring visualization, video, and gamification. Thus, the development of Canva-based media integrated with Wordwall is deemed relevant for enhancing learning interest and student engagement.