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The Utilization of Native Freshwater Mussel Pilsbryoconcha exilis as Biocontrol of Pathogenic Bacteria Aeromonas hydrophila in Tilapia Aquaculture Rahman Rahman; Filibertus Tantio; Munti Yuhana; Amalia Putri Firdausi; Ranta Sumadi; Adna Sumadikarta
Journal Omni-Akuatika Vol 15, No 2 (2019): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

This research aims to evaluate the capacity of freshwater mussel Pilsbryoconcha exilis as a biocontrol agent to prevent the transmission of Aeromonas hydrophila in tilapia cultivation. Briefly, 10 tilapia fish with average bodyweight 7,88±0,25 g were subjected to four treatments in the 8-liter aquarium with three replications in a randomized design trial. The treatments were M1 (one mussel for a two-liter of water), M2 (two mussels for a two-liter of water), and two control treatments without mussel ( M+ and M-). All treatments, except the M-, then challenged by adding A. hydrophila live culture to obtain a final density of 105 CFU mL-1 into the aquarium for 7 days duration. The final survival rate of fish, the water-total bacterial count, and the blood profile of animals were assessed. The research revealed that there is a significant impact from the presence of freshwater mussel on tilapia cultivation. Generally, the M2 treatment showed better results with a significant different (P<0,05) according to the survival rate of fish (100±0,00%), water-total bacterial count (4,53±0,03 log CFU mL-1), and fish leucocytes (4,30±0,70x104 cell mm-3). Nonetheless, there was no different (P>0,05) effect on fish erythrocytes among the treatments. Therefore, the feeding activity of freshwater mussel in the water column able to deplete pathogenic bacteria abundance and prevent pathogen transmission along with increasing the survival rate of fish.

PROTOZOA EKTOPARASITIK PADA IKAN KOI Cyprinus carpio DI DAERAH SUKABUMI Amalia Putri Firdausi; Rahman Rahman; Respati Mahadhika; Adna Sumadikarta
Jurnal Akuakultur Rawa Indonesia Vol 8, No 1 (2020): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

The aim of this study is to inventory various types of ectoparasites found in koi fish that are reared in the nursery phase at the Sukabumi Freshwater Aquaculture Center (BPAT Sukabumi). Briefly, a total of 30 koi fish were taken randomly, then the scales, fins, and gills were examined and stained either with procedures of AgNO3. The type of parasite was identified and analyzed based on the rate of prevalence, also the intensity and dominance. The infesting parasite found were Trichodina sp, Oodinium sp, and Ichthyophthirius multifiliis. Overall, Trichodina sp is the most dominating parasite with a dominance level, prevalence, and intensity of 57,028%, 80%, and 10,833 respectively.

Ploidy level determination in genetically modified polyploid striped catfish Pangasianodon hypophthalmus Sauvage, 1878 based on the number of nucleoli per cell Muhammad Sami Daryanto; Odang Carman; Dinar Tri Soelistyowati Soelistyowati; Rahman Rahman
Jurnal Iktiologi Indonesia Vol 19 No 1 (2019): February 2019
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v19i1.405

The variation in the maximum number of nucleoli per cell in diploid and tetraploid striped catfish Pangasianodon hypophthalmus Sauvage, 1878 in attempts to verify the validity of nucleoli counting as an indirect method for polyploidy identification in fish. The aims of this research is to determine ploidy level of striped catfish based on maximum number of nucleoli per cell. Diploid striped catfish was produced by fertilizing eggs without thermal-shock and tetraploid fish was obtained by fertilizing eggs and applying thermal-shock induction with 4oC for 25 minutes at zygotic age 28 minutes after fertilization prior to first cleavage stage of zygote. The hatching rate of diploid group and tetraploid group were 81,35±0,73% and 3,39±1,78% and survival rate during 15 days rearing were 88,67±5,25% and 83,33±5,73%, respectively. The frequency of one, two, three, and four nucleoli per cell were counted based on each sample observation of 450-550 cells. Cells of diploid individuals had one, and maximum two nucleoli per cell, while tetraploid there were one, two, three, and maximum four nucleoli per cell. Anomaly in the silver-stained appearance at the maximum number of three nucleoli per cell indicated tetraploid individuals as verified by chromosome counting method. Ploidy level determination of striped catfish using the number of nucleoli per cell has a potential for rapid identification. Abstrak Penentuan variasi jumlah maksimum nukleolus per sel pada individu diploid dan tetraploid patin siam Pangasianodon hypophthalmus Sauvage, 1878 dilakukan dalam upaya verifikasi ketepatan penghitungan nukleolus sebagai metode tidak langsung dalam identifikasi ikan poliploid. Penelitian ini bertujuan menentukan tingkat ploidi ikan patin siam berdasarkan jumlah maksimum nukleolus per sel. Diploid patin siam dihasilkan melalui fertilisasi buatan antara telur dan sperma tanpa kejutan suhu sedangkan tetraploid patin siam dihasilkan melalui fertilisasi antara telur dan sperma serta di induksi menggunakan kejutan suhu 4oC dengan durasi kejutan 25 menit pada umur zigot 28 menit setelah fertilisasi sesaat sebelum mitosis I. Persentase penetasan kelompok perlakuan diploid 81,35±0,73% dan kelompok perlakuan tetraploid 3,39±1,78%. Sintasan kelompok perlakuan diploid 88,67±5,25% dan kelompok tetraploid 83,33±5,73% selama 15 hari pemeliharaan. Frekuensi satu, dua, tiga dan empat nukleoli per sel dihitung berdasarkan pengamatan 450-550 sel setiap individu yang diamati. Sel individu diploid memiliki satu dan maksimum dua nukleoli per sel, sedangkan individu tetraploid memiliki satu, dua, tiga, dan maksimum empat nukleoli per sel. Anomali muncul melalui pewarnaan perak nitrat pada jumlah maksimum tiga nukleoli per sel menunjukkan individu tersebut tetraploid yang diverifikasi menggunakan metode penghitungan kromosom. Penentuan ploidi pada ikan patin siam menggunakan penghitungan jumlah nukleolus per sel memiliki potensi dalam identifikasi secara cepat.

Study on antifungal potency of Terminalia cattapa, Piper betle, Psidium guajava, and Andrographis peniculata on the growth of Aphanomyces in vitro Nuryati, Sri; Rahman, .; Taukhid, .
Jurnal Akuakultur Indonesia Vol. 4 No. 2 (2005): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

An effort to prevent aquatic fungi Aphanomyces sp. infection on fish using natural material can be an economically way, easy to find the materials, easy to apply and safe for environment. The antifungal potency and efficacy of scalded-leaf extract of Terminalia cattapa, Piper betle, Psidium guajava and Andrographis peniculata on prevention of Aphanomyces sp. growth in vitro in GYA medium. Scalding was performed in the water at 50°C. Concentration of leaf extracts tested was 0, 10, 20, 40 and 80 g/L. The results of study showed that Terminalia cattapa in a dosage of 40 g/L had the best prevention activity, followed by Piper betle in the same dosage. Psidium guajava and Andrographis peniculata had no prevention activity on growth of Aphanomyces sp. Keywords: antifungal, Terminalia cattapa, Piper betle, Psidium guajava, Andrographis peniculata growth, Aohanomyces sp. ABSTRAK Upaya penanggulangan infeksi cendawan akuatik Aphanomyces sp. pada ikan menggunakan bahan alami dapat menjadi cara yang ekonomis ekonomis, bahan mudah didapat, mudah diterapkan dan aman bagi lingkungan. Potensi antifungi dan efektivitas ekstrak seduh daun ketapang (Terminalia cattapa), sirih (Piper betle), jambu biji (Psidium guajava) dan sambiloto (Andrographis peniculata) terhadap penghambatan pertumbuhan Aphanomyces sp. dilakukan secara in vitro dalam media biakan GYA. Penyeduhan dilakukan menggunakan pelarut air dengan suhu 50°C. Konsentrasi yang diuji adalah 0, 10, 20, 40 dan 80 gr/L untuk masing-masing bahan. Aktivitas penghambatan paling baik terhadap cendawan diperoleh dari ekstrak seduh daun ketapang 40 g/L dan diikuti oleh ekstrak seduh daun sirih dengan konsentrasi yang sama. Jambu biji dan sambiloto tidak menunjukkan aktivitas penghambatan terhadap pertumbuhan Aphanomyces sp. Kata kunci: antifungi, ketapang, sirih, jambu biji, sambiloto dan Aphanomyces sp.

Effectivity of Musa paradisiaca extract to control Saprolegnia sp. infection on giant gourami larvae Nuryati, Sri; Aulia, Nadia; Rahman, ,
Jurnal Akuakultur Indonesia Vol. 14 No. 2 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Larval stage of giant gourami is a critical period due to fungal infection, such as Saprolegnia sp. infection. There are some plants which have antiseptic compound like banana Musa paradisiaca. This research was aimed to examine the effectiveness of the banana stem extract M. paradisiaca to control Saprolegnia sp. infection on giant gurami larvae through immersion. Eight-day old gorami larvae (at the initial of 0.5±0.03 cm) was reared in an aquarium sized 25×25×25 cm3 at the density of 8 fry/L. Culture media were added banana stem extract at the dose of 0; 0.08; 0.12; and 0.16 g/L during 21 days of rearing period. Challenge test was performed for 14 days by giving Saprolegnia sp. spores at the density of 104 cells/mL and banana stem extract. The treatment dose of 0.16 g/L has showen survival 100% than positive control after the challenge test. Keywords: giant gourami, Musa paradisiaca, Saprolegnia sp., fry ABSTRAK Fase larva ikan gurami merupakan masa kritis terhadap infeksi cendawan, seperti jenis Saprolegnia sp. Beberapa tanaman memiliki daya antiseptik seperti tanaman pisang ambon Musa paradisiaca. Penelitian ini bertujuan untuk menguji efektivitas ekstrak batang pisang ambon M. paradisiaca dalam mengurangi infeksi Saprolegnia sp. pada larva ikan gurami melalui media pemeliharaan. Larva gurami umur delapan hari (panjang larva 0,5+0,03 cm) dipelihara pada akuarium berukuran 25×25×25 cm3 dengan padat tebar 8 ekor/L. Media pemeliharaan diberi ekstrak batang pisang ambon dosis 0; 0,08; 0,12; dan 0,16 g/L selama 21 hari. Uji tantang dilakukan selama 14 hari dengan pemberian spora Saprolegnia sp. kepadatan 104 sel/mL dan ekstrak batang pisang ambon. Perlakuan dosis 0,16 g/L memberikan kelangsungan hidup sebesar 100% yang lebih tinggi dibandingkan perlakuan kontrol positif setelah uji tantang. Kata kunci: giant gourami, Musa paradisiaca, Saprolegnia sp., larva

Infektivitas parasit Ichtyophthirius multifiliis yang disimpan pada suhu rendah Rahman, ,; Sukenda, ,; Nuryati, Sri; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 15 No. 2 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The aim of this study was to evaluate infectivity of Ichtyophthirius multifiliis which caused white spot disease maintained at low temperature without its host. Briefly, the trophont stage of parasites were subjected at control (28 °C) and lower temperature (9 °C) for 14 consecutive days of observation. The rate of survival, and excystment of descendants were examined descriptively at the last day of observation. Here, the infectivity of parasite then performed by means infecting the model fish Poecilia sphenops (black moly) with escaping theronts. The results revealed that the survival rate and excystment rate of parasite were decreased as maintaining period increased. The final rate of survival, and excystment of parasite were 35% and 33,3% respectively. Additionally, the descendants came out with high abnormality which recognized by weak mobility and lower infectivity (50%) compared to the control (80%). Then, it is concluded that, maintaining I. multifiliis at low temperature without its host for 14 consecutive days will decreased the infectivity. Keywords: white spot, obligat parasite, excystment, infectivity ABSTRAK Penelitian ini bertujuan untuk mengevaluasi infektivitas parasit Ichtyophthirius multifiliis penyebab penyakit bintik putih (white spot) yang dipelihara tanpa inang pada suhu rendah. Parasit dengan stadia trophont dipelihara pada suhu ruang (28 °C) dan suhu rendah (9 °C) selama 14 hari. Selama masa pemeliharaan tersebut tingkat kelulusan hidup, dan tingkat eksismen parasit diukur dan dibandingkan secara deskriptif. Hari terakhir pemeliharaan dilakukan uji tantang pada ikan black moly Poecilia sphenops untuk menilai infektivitas parasit. Hasil penelitian memperlihatkan bahwa tingkat kelulusan hidup dan eksismen parasit semakin menurun dengan bertambahnya masa pemeliharaan. Akhir pengamatan kelangsungan hidup, dan nilai eksismen tersebut berturut-turut adalah 35% dan 33%. Parasit yang disimpan pada suhu rendah selama 14 hari memperlihatkan infektivitas yang lebih rendah (50%) dibandingkan dengan perlakuan kontrol (80%). Kesimpulannya, penyimpanan parasit I. multifiliis pada suhu rendah selama 14 hari dapat menurunkan infektivitas parasit pada inang. Kata kunci: bintik putih, parasit obligat, eksismen, infektivitas

Efikasi daun sembukan Paederia foetida untuk pencegahan infeksi bakteri Aeromonas hydrophila pada ikan nila Wahjuningrum, Dinamella; Hasanah, Mulyati; Rahman, ,
Jurnal Akuakultur Indonesia Vol. 15 No. 2 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The purpose of this study was to determine the optimum dosage of skunkvine leaves Paederia foetida to prevent infection caused by bacteria Aeromonas hydrophila in tilapia Oreochromis niloticus. This study consisted of five treatments. They were negative control, positive control, and prevention treatment with the dosages of 0.8%, 1% and that 1.2% that consisted that of three replications in each treatment. Addition of skunkvine leaves on feed performed by coating method. Feed was given at satiation with a frequency of three times a day. The results of this study showed that there were significant effect (P<0.05) between the positive control (37.03%) and preventive treatment dosages of 0.8% (88.89%), 1% (74.08%), and 1.2% (74.08%). The optimum dosage for prevention of A. hydrophila infection in tilapia was 0.8%. Keywords: Aeromonas hydrophila, Paederia foetida, tilapia ABSTRAK Tujuan penelitian ini adalah untuk menentukan dosis daun sembukan Paederia foetida yang tepat dalam mencegah infeksi akibat Aeromonas hydrophila pada ikan nila Oreochromis niloticus. Penelitian ini terdiri dari lima perlakuan yaitu kontrol negatif, kontrol positif, perlakuan 0,8%, perlakuan 1%, dan perlakuan 1,2% dengan masing-masing perlakuan terdiri dari tiga ulangan. Metode penambahan sembukan pada pakan dilakukan dengan metode coating. Pakan diberikan secara at satiation dengan frekuensi pemberian sebanyak tiga kali sehari. Pemeliharaan ikan dilakukan selama 20 hari dan pada hari ke-11 dilakukan uji tantang dengan menggunakan bakteri A. hydrophila. Hasil penelitian ini menunjukan bahwa tingkat kelangsungan hidup sebelum uji tantang tidak berbeda nyata (P>0,05), sedangkan setelah uji tantang diperoleh hasil yang berbeda nyata (P<0,05) antara kontrol positif (37,03%) dengan perlakuan 0,8% (88,89%), perlakuan 1% (74,08%), dan perlakuan 1,2% (74,08%). Berdasarkan hasil penelitian disimpulkan bahwa pemberian daun sembukan melalui pakan efektif untuk pencegahan infeksi A. hydrophila pada ikan nila dengan dosis terbaik yaitu 0,8%. Kata kunci: Aeromonas hydrophila, Paederia foetida, ikan nila

Kinerja probiotik Bacillus sp. pada pendederan benih ikan lele Clarias sp. yang diinfeksi Aeromonas hydrophila Sukenda, ,; Rafsyanzani, Muhammad Mufthi; Rahman, ,; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 15 No. 2 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This experiment was conducted to assess performance of Bacillus sp. probiotic on catfish juvenile Clarias sp. infected by Aeromonas hydrophila. The probiotic content in the diets were 0% (K+ and K-), 1%, and 2% in duplicates. This experiment used randomized design with four treatments and two replications. Juveniles with average body weight of 3.22±0.15 g/fish were reared in the 1.5×2.8×0.5 m3 pond with density of 800 fish/pond. Fish were reared for 30 days and fed three times a day at rate 8% of total body weight. At day 31, catfish were challenged by A. hydrophila 0.1 mL (106 cfu/mL). Post infection observation was carried out ten days with density 10 fish/aquaria. The result showed that fish fed diet containing 2% probiotic gave the best probiotic performance with survival rate of catfish 83.33% after challenged, spesific growth rate 5.40%, and 0,75 of feed conversion ratio. The results of the blood profile showed significantly better results in the treatment of probiotics compared to the positive control after challenge test A. hydrophila. Probiotic Bacillus sp. has given as much as 2% on feed provides better performance on catfish juvenile. Keywords: probiotic, Bacillus sp., A. hydrophila, catfish juvenille, growth ABSTRAK Penelitian ini bertujuan untuk menguji kinerja probiotik Bacillus sp. dalam pakan pada pendederan benih ikan lele Clarias sp. yang diinfeksi bakteri Aeromonas hydrophila. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan yaitu kandungan probiotik dalam pakan perlakuan yaitu 0% (K+ dan K-), 1%, dan 2%, masing-masing dengan dua ulangan. Ikan lele yang digunakan memiliki bobot rata-rata 3,22±0,15 g/ekor, dipelihara dalam kolam terpal berukuran 1,5×2,8×0,5 m3 dengan kepadatan 800 ekor/kolam. Ikan dipelihara selama 30 hari dengan frekuensi pemberian pakan tiga kali sehari sebanyak 8% dari bobot tubuh ikan. Hari ke-31 benih lele diinjeksi bakteri A. hydrophila dosis 0,1 mL/ekor dengan kepadatan bakteri 106 cfu/mL. Pemeliharaan setelah diinfeksi dilakukan selama sepuluh hari dengan kepadatan 10 ekor/akuarium. Hasil penelitian menunjukkan ikan yang diberi probiotik 2% memperlihatkan kinerja probiotik terbaik dengan tingkat kelangsungan hidup ikan lele sebesar 83,33% setelah diinfeksi dengan A. hydrophila; laju pertumbuhan harian sebesar 5,40%; dan konversi pakan 0,75. Hasil gambaran darah menunjukkan hasil yang signifikan lebih baik pada perlakuan pemberian probiotik dibandingkan kontrol positif pascauji tantang A. hydrophila. Probiotik Bacillus sp. yang diberikan sebanyak 2% pada pakan memberikan kinerja lebih baik pada pendederan benih ikan lele. Kata kunci: probiotik, Bacillus sp., A. hydrophila, benih lele, pertumbuhan

Efficacy of whole cell vaccine Aeromonas hydrophila on catfish broodstock and it’s offspring resistance againt motile aeromonad septicemia (MAS) Sukenda, ,; Pratiwi, Kiki Amalia; Rahman, ,; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 16 No. 1 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Transfer of maternal immunity by mean of passive immunization is a way to provide protection and durability of antibodies on the offspring. The purpose of this research was to analize effication of Aeromonas hydrophila vaccine on the catfish broodstock to maternal transfer of immunity, and offspring resistance. The average body weight of broodstock used in this study were 650±50 g were kept in pool tarps sized 2×1×0.5 m3. This study used a randomized complete design with two treatments and three replications. Female broodstock were vaccinated using intraperitonial injections at a dose 0.4 mL/kg and control fish were injected with phospate buffered saline (PBS). The observed parameters include hematology of broodstock, mortality, the relative survival rate, and antibody titers. Antibody titer measurements on broodstock, eggs, and offspring. Vaccination on broodstock catfish delivers a significant antibody level (P<0.05) on offspring compared to control catfish with relative survival rate of offspring at 5, 10, and 15 days after hatching were 67.76%, 82.66%, and 71.66% respectively. Keywords: catfish, Aeromonas hydrophila, vaccination, antibody transfer ABSTRAK Transfer kekebalan dari induk kepada benih melalui imunisasi pasif merupakan salah satu cara untuk memberikan proteksi pada benih. Tujuan penelitian ini untuk menguji efikasi vaksin sel utuh Aeromonas hydrophila pada induk ikan nila dalam mentransfer kekebalan spesifik ke benih dan menguji ketahanan benih hasil pemijahan induk yang divaksin. Induk lele yang digunakan pada penelitian ini memiliki bobot rata-rata 650±50 g dipelihara di kolam terpal berukuran 2×1×0,5 m3. Penelitian ini menggunakan rancangan acak lengkap dengan dua perlakuan dan tiga ulangan. Induk betina lele divaksinasi secara intraperitonial dengan dosis 0,4 mL/kg ikan dan induk lele kontrol disuntik dengan phospate buffer saline (PBS). Parameter yang diamati meliputi hematologi induk, mortalitas, tingkat kelangsungan hidup relatif benih, dan titer antibodi. Vaksinasi induk lele memberikan hasil level antibodi yang signifikan (P<0,05) pada induk, telur, dan benih lele dibandingkan perlakuan kontrol dengan tingkat kelangsungan hidup relatif benih umur 5, 10, dan 15 hari pacatetas masing-masing sebesar 67,76; 82,66%; dan 71,66%. Kata kunci: ikan lele, Aeromonas hydrophila, vaksinasi, transfer antibodi

Efficacy of Streptococcus agalactiae vaccine strains N3M and N4M in fry tilapia infected by different strains of S. agalactiae Sukenda, Sukenda,; Firmansyah, Arif Lukman; Rahman, Rahman,; Nuryati, Sri; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 17 No. 2 (2018): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Streptococcus agalactiae is a major bacterial streptococcosis disease that infects tilapia. This study aimed to analyze a specific and nonspecific immune system in fry tilapia that has been given with S. agalactiae vaccine from N3M and N4M strain and examine the protective immunity against S. agalactiae N3M, N4M, N17O, NK1, and N14G strains infection. Fry tilapia used in this study has the weight of 7.086±0.948 g and length of 7.443±0.353 cm. The S. agalactiae strains that used were N3M, N4M, N17O, NK1,and N14G. Fry tilapia was vaccinated through intraperitoneal injection method with 0.1 mL per fish of N3M and N4M vaccines. Fish reared in aquarium sizing of 60×30×50 cm3 with a density of 10 fishes aquarium-1. Two weeks after vaccination, fry tilapia was tested with 0.1 mL fish-1 of each N3M, N4M, N17O, NK1, and N14G strains through intraperitoneal injection method. Antibody level measured with indirect enzym-linked immunosorbent assay (ELISA) method. The result of antibody level in vaccinated fish after vaccination of N3M, N4M vaccine, N3M and N4M control were 0.767; 0.743; 0.587; and 0.544, respectively. Relative percent survival in N3M vaccinated fish after challenged with N3M and N4M was 87.50% dan 64.70%, respectively, otherwise in N4M vaccinated fish was 62.50% dan 76.47%, respectively. N3M and N4M vaccine strain have better protection as only if it tested with similar bacteria strain. Keywords: formalin-killed cell, tilapia, protection, Streptococcus agalactiae, strains ABSTRAK Streptococcus agalactiae merupakan bakteri utama penyakit streptococcosis yang menginfeksi ikan nila. Tujuan penelitian ini adalah menganalisis sistem imun spesifik dan nonspesifik pada benih ikan nila yang diberi vaksin S. agalactiae strain N3M dan N4M serta mengkaji imunitas protektif terhadap infeksi S. agalactiae strain N3M, N4M, N17O, NK1, dan N14G. Benih ikan nila yang digunakan memiliki bobot 7,086±0,948 g dan panjang 7,443±0,353 cm. Bakteri yang digunakan adalah S. agalactiae strain N3M, N4M, N17O, NK1,danN14G. Benih ikan nila divaksinasi menggunakan metode injeksi pada bagian intraperitoneal sebanyak 0,1 mL ekor-1 vaksin N3M dan N4M. Pemeliharaan dilakukan pada akuarium berukuran 60×30×50 cm3 dengan kepadatan 10 ekor akuarium-1. Dua minggu setelah vaksinasi benih ikan diuji tantang dengan strain bakteri N3M, N4M, N17O, NK1, danN14G menggunakan metode injeksi pada bagian intraperitoneal dengan dosis 0,1 mL ekor-1. Level antibodi diukur dengan metode indirect enzym-linked immunosorbent assay (ELISA). Hasil menunjukkan level antibodi ikan setelah vaksinasi pada perlakuan vaksin N3M, N4M, kontrol N3M, dan kontrol N4M berturut-turut 0,767; 0,743; 0,587; dan 0,544. Kelangsungan hidup relatif ikan yang divaksin N3M setelah diuji tantang dengan strain N3M dan N4M berturut-turut 87,50% dan 64,70%, sedangkan pada ikan yang divaksin N4M berturut-turut 62,50% dan 76,47%. Vaksin strain N3M dan N4M memiliki proteksi lebih baik jika diuji tantang dengan strain bakteri yang sama. Kata kunci: formalin-killed cell, Streptococcus agalactiae, strain, proteksi, nila

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